Recombinant DNA-directed RNA polymerase II subunit RPB1 (RPB1)

中文名稱：

Recombinant DNA-directed RNA polymerase II subunit RPB1(RPB1)
貨號：

CSB-YP018327EPO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant DNA-directed RNA polymerase II subunit RPB1(RPB1)
貨號：

CSB-EP018327EPO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant DNA-directed RNA polymerase II subunit RPB1(RPB1)
貨號：

CSB-EP018327EPO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant DNA-directed RNA polymerase II subunit RPB1(RPB1)
貨號：

CSB-BP018327EPO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant DNA-directed RNA polymerase II subunit RPB1(RPB1)
貨號：

CSB-MP018327EPO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

RPB1
Uniprot No.：

P28364
別名：

RPB1; DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; EC 2.7.7.6; DNA-directed RNA polymerase III largest subunit; Fragment
種屬：

Euplotes octocarinatus
蛋白長度：

full length protein
表達區域：

1-478
氨基酸序列

MSRGTIYTES TMDFQKVRKI QFGLLDPKEI QAMSVVQVEN EKIYDNGIPT DGGINDLRMG TMEKAMRCST CQGDSKECPG HFGHIELAQP VFHIGFIDLV KKILKCVCFN CNKLLITDKH DKYSALKRVK DPKLKLNKVY KVCKDIKVCG KADRKSETYT EGSGQKQPRL RKTGLKIKAE FPIDEDDPST NDNKRDLSAS ECLKILGRIS PDDCKFLGFD MVLARPEWLI ISRLPVAPPP VRPSVCMGSN IRQEDDLTHQ YQQILKANNQ LRKHLSTANH IINENYQLLQ FYCATLIDNE QAGQMVSRHK SGGKAIKAIR ARLKGKEGRL RGNLMGKRVD FSARTVITCD PTLDLDQLGV PRSIAENITI PEVVTPQNID EMRKLVINGP NKWPGAKYIK GEGGKMIDLS YAKTTETFID YGYVIERHLK NDDFVLFNRQ PSLHKMSIMG HRVKVLPYST FRLNLSVTAP YNADFDGS
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.
亞細胞定位：

Nucleus.
蛋白家族：

RNA polymerase beta' chain family