Recombinant Bovine E3 ubiquitin-protein ligase TRIM21 (TRIM21)

中文名稱：

牛TRIM21重組蛋白
貨號：

CSB-YP768741BO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

牛TRIM21重組蛋白
貨號：

CSB-EP768741BO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

牛TRIM21重組蛋白
貨號：

CSB-EP768741BO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

牛TRIM21重組蛋白
貨號：

CSB-BP768741BO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

牛TRIM21重組蛋白
貨號：

CSB-MP768741BO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

TRIM21
Uniprot No.：

Q7YRV4
別名：

TRIM21; E3 ubiquitin-protein ligase TRIM21; EC 2.3.2.27; 52 kDa Ro protein; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; RING-type E3 ubiquitin transferase TRIM21; Ro(SS-A); Sjoegren syndrome type A antigen; SS-A; Tripartite motif-containing protein 21
種屬：

Bos taurus (Bovine)
蛋白長度：

full length protein
表達區域：

1-469
氨基酸序列

MASAVPLTMM WEEVTCSICL DPMVEPMSIE CGHSFCQECI SEVGKEGGSV CPVCRRHFLL QNLRPNRQVA NMVDNLRKIS QGAKESPHGE LCVVHREKIH LFCEEDGKAL CWVCSQSQKH RDHPMVPIEE AAQEYQEKLQ VALNKLRHKQ ELAEKLELDI AMKKASWKGK VEAHKLRIHE EFVRQKNFLA EEEQKQLQKL EEEERQQLRT LGDTEARLAQ QIQALQELIA ELERRRRGSA LELLQEVKSV LERSESWNLK ELDVASTDLR NVFYVPGIKK MLRTCGVHIT LDPQTANPWL ILSENRRQVR LANTRQEVPE NEERFDSYPM VLGAQRFDSG KVYWEVDVTG KEAWDLGVCR DNVRRKGQFL LNSDTGFWII WLWNKQKYEA GTCPQTPLHL QVPPNRIGIF LDYEASTVSF YNITDHASLI YTFSECAFAG PLRPFFNPGF NDRGTNSAPL ILCPLKTGW
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1 and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of IRF3, hence attenuating type I interferon (IFN)-dependent immune responses. Represses the innate antiviral response by facilitating the formation of the NMI-IFI35 complex through 'Lys-63'-linked ubiquitination of NMI.
基因功能參考文獻：
1. The present studies describe the expression cloning of this gene product from a bovine brain microvascular cDNA library. PMID: 14534436
亞細胞定位：

Cytoplasm. Cytoplasmic vesicle, autophagosome. Nucleus. Cytoplasm, P-body.
蛋白家族：

TRIM/RBCC family
數據庫鏈接：

KEGG: bta:359715

UniGene: Bt.106989