Recombinant Bacillus subtilis NAD-specific glutamate dehydrogenase (rocG)

中文名稱：

枯草芽孢桿菌rocG重組蛋白
貨號：

CSB-YP336603BRJ
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

枯草芽孢桿菌rocG重組蛋白
貨號：

CSB-EP336603BRJ
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

枯草芽孢桿菌rocG重組蛋白
貨號：

CSB-EP336603BRJ-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

枯草芽孢桿菌rocG重組蛋白
貨號：

CSB-BP336603BRJ
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

枯草芽孢桿菌rocG重組蛋白
貨號：

CSB-MP336603BRJ
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

rocG
Uniprot No.：

P39633
別名：

rocG; gudA; yweB; BSU37790; ipa-75dCatabolic NAD-specific glutamate dehydrogenase RocG; NAD-GDH; EC 1.4.1.2; Glutamate dehydrogenase; GlutDH; Trigger enzyme RocG
種屬：

Bacillus subtilis (strain 168)
蛋白長度：

full length protein
表達區域：

1-424
氨基酸序列

MSAKQVSKDE EKEALNLFLS TQTIIKEALR KLGYPGDMYE LMKEPQRMLT VRIPVKMDNG SVKVFTGYRS QHNDAVGPTK GGVRFHPEVN EEEVKALSIW MTLKCGIANL PYGGGKGGII CDPRTMSFGE LERLSRGYVR AISQIVGPTK DIPAPDVYTN SQIMAWMMDE YSRLREFDSP GFITGKPLVL GGSQGRETAT AQGVTICIEE AVKKKGIKLQ NARIIIQGFG NAGSFLAKFM HDAGAKVIGI SDANGGLYNP DGLDIPYLLD KRDSFGMVTN LFTDVITNEE LLEKDCDILV PAAISNQITA KNAHNIQASI VVEAANGPTT IDATKILNER GVLLVPDILA SAGGVTVSYF EWVQNNQGYY WSEEEVAEKL RSVMVSSFET IYQTAATHKV DMRLAAYMTG IRKSAEASRF RGWV
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Devoted to catabolic function of glutamate (and other amino acids of the glutamate family) utilization as sole nitrogen source. It is not involved in anabolic function of glutamate biosynthesis since B.subtilis possesses only one route of glutamate biosynthesis from ammonia, catalyzed by glutamate synthase. RocG is unable to utilize glutamate or glutamine as sole carbon source and to synthesize glutamate, but it is involved in the utilization of arginine, and proline as carbon or nitrogen source. The catabolic RocG is essential for controlling gltAB expression via an inhibitory interactions with the transcriptional regulator GltC in response to the availability of sugars.
基因功能參考文獻：
1. The authors conclude that RocG glutamate dehydrogenase activity affects the expression of the sigma(W) regulon, by pathways that are yet unclear. PMID: 22178969
2. Glutamate dehydrogenase cloned from the rocG gene was characterized for thermostability, oligomerization, and substrate specificity. Random mutagenesis produced an enzyme with missense mutation that enhanced thermostability and k(cat). PMID: 16244435
3. Regulated expression of HPrK/P does not affect carbon catabolite repression of rocG PMID: 16684115
4. The genetic and biochemical data presented here show that the glutamate dehydrogenase RocG is able to bind to and concomitantly inactivate the activator protein GltC. PMID: 17608797
蛋白家族：

Glu/Leu/Phe/Val dehydrogenases family
數據庫鏈接：

KEGG: bsu:BSU37790

STRING: 224308.Bsubs1_010100020411