Recombinant Avian infectious bronchitis virus Spike glycoprotein (S), partial

中文名稱：

Recombinant Avian infectious bronchitis virus Spike glycoprotein(S) ,partial
貨號：

CSB-YP319267ARN
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Avian infectious bronchitis virus Spike glycoprotein(S) ,partial
貨號：

CSB-EP319267ARN
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Avian infectious bronchitis virus Spike glycoprotein(S) ,partial
貨號：

CSB-EP319267ARN-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Avian infectious bronchitis virus Spike glycoprotein(S) ,partial
貨號：

CSB-BP319267ARN
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Avian infectious bronchitis virus Spike glycoprotein(S) ,partial
貨號：

CSB-MP319267ARN
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

S
Uniprot No.：

P11223
別名：

S; 2; Spike glycoprotein; S glycoprotein; E2; Peplomer protein) [Cleaved into: Spike protein S1; Spike protein S2; Spike protein S2']
種屬：

Avian infectious bronchitis virus (strain Beaudette) (IBV)
蛋白長度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

attaches the virion to the host cell membrane by interacting with sialic acids, initiating the infection.; mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.; Acts as a viral fusion peptide after S2 cleavage occurring upon virus endocytosis.
基因功能參考文獻(xiàn)：
1. The observation that IBV Beaudette has the additional tropism for growth on Vero cells also invokes the possibility of generating IBV vaccines produced from cultured cells rather than by the use of embryonated eggs. The regions of the IBV Beaudette S glycoprotein involved in the determination of extended cellular tropism were identified in this study. PMID: 30021894
2. In this study, the authors used bioinformatics tools to predict N-linked glycosylation sites and to analyze their distribution in infectious bronchitis virus strains and variants. Among these sites, 8 sites were confirmed in the S protein extracted from partially purified virus particles by proteomics approaches. PMID: 29035787
3. Infectious bronchitis virus (IBV) S1 amino acid sequences showing > or = 95.8% amino acid identity to the S1 of the challenge strain differed in their ability at conferring protection. PMID: 25055623
4. S2 domain of spike protein does not contain an additional independent receptor-binding site. PMID: 24041648
5. Critical amino acids for receptor attachment of spike protein are within the N-terminal residues 19-69. PMID: 24314633
6. Binding of the infectious bronchitis virus spike protein to host cells was in all experiments sialic acid dependent. PMID: 23896748
7. Two isolates of infectious bronchitis virus from outbreaks in 2008 and 2010 in broiler chickens in India were isolated and characterized. Reverse transcription PCR-RFLP of the entire S1 gene revealed that these isolates belong to two different genotypes. PMID: 22573006
8. The homology of nucleotide and amino acid sequences of the S1 gene among seven isolates was 81.7%-99.7% and 79.0%-99.4%, respectively. These strains were genetically different from vaccine strains and closely related to large numbers of Chinese strains. PMID: 22017046
9. Data indicate that strains within the 793/B serotype have > or =96% nucleotide identity within the whole S1 part of the spike protein gene. PMID: 15763735
10. Mutations and recombination events could contribute to the genetic diversity of the Italian isolates. PMID: 17043759
11. Analysis of the nucleotide and deduced amino acid sequences revealed that the eight strains from New Zealand are genetically related and share greater than 82.8% nucleotide and 79% amino acid homology within the S1 region. PMID: 18712588
12. Taken together, these results suggest that proteolysis at a novel XXXR/S motif in the S2 subunit might be a common mechanism for the entry of CoV into cells. PMID: 19553314
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亞細(xì)胞定位：

[Spike protein S2]: Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein.; [Spike protein S1]: Virion membrane; Peripheral membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Peripheral membrane protein.
蛋白家族：

Gammacoronaviruses spike protein family
數(shù)據(jù)庫鏈接：

KEGG: vg:1489741