Recombinant Arabidopsis thaliana Wee1-like protein kinase (WEE1)

中文名稱：

擬南芥WEE1重組蛋白
貨號：

CSB-YP811852DOA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

擬南芥WEE1重組蛋白
貨號：

CSB-EP811852DOA
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

擬南芥WEE1重組蛋白
貨號：

CSB-EP811852DOA-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

擬南芥WEE1重組蛋白
貨號：

CSB-BP811852DOA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

擬南芥WEE1重組蛋白
貨號：

CSB-MP811852DOA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

WEE1
Uniprot No.：

Q8L4H0
別名：

WEE1; At1g02970; F22D16.3; Wee1-like protein kinase; EC 2.7.10.2; Wee1-At
種屬：

Arabidopsis thaliana (Mouse-ear cress)
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-500
氨基酸序列

MFEKNGRTLL AKRKTQGTIK TRASKKIRKM EGTLERHSLL QFGQLSKISF ENRPSSNVAS SAFQGLLDSD SSELRNQLGS ADSDANCGEK DFILSQDFFC TPDYITPDNQ NLMSGLDISK DHSPCPRSPV KLNTVKSKRC RQESFTGNHS NSTWSSKHRV DEQENDDIDT DEVMGDKLQA NQTERTGYVS QAAVALRCRA MPPPCLKNPY VLNQSETATD PFGHQRSKCA SFLPVSTSGD GLSRYLTDFH EIRQIGAGHF SRVFKVLKRM DGCLYAVKHS TRKLYLDSER RKAMMEVQAL AALGFHENIV GYYSSWFENE QLYIQLELCD HSLSALPKKS SLKVSEREIL VIMHQIAKAL HFVHEKGIAH LDVKPDNIYI KNGVCKLGDF GCATRLDKSL PVEEGDARYM PQEILNEDYE HLDKVDIFSL GVTVYELIKG SPLTESRNQS LNIKEGKLPL LPGHSLQLQQ LLKTMMDRDP KRRPSARELL DHPMFDRIRG
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Cell cycle regulatory kinase that is not rate-limiting for cycle progression under normal growth conditions. Transcriptionally activated upon DNA stress or damage in an ATR- or ATM-dependent manner. Once activated, inhibits plant growth by arresting dividing cells in the G2 phase before proceeding into mitosis. Down-regulates CDKA-1 and CDKD-2 by tyrosine phosphorylation. May target principally CDKA-1.
基因功能參考文獻(xiàn)：
1. the identification of a second member of the RNase H2 complex, RNase H2B, being able to complement the root growth phenotype of WEE1(KO) plants, is reported. PMID: 25875879
2. The plant WEE1 kinase acquired an indirect developmental function that is important for meristem maintenance upon replication stress. PMID: 21498679
3. The WEE1 gene is not rate-limiting for cell cycle progression under normal growth conditions but is a critical target of the ATR-ATM signaling cascades that inhibit the cell cycle upon activation of the DNA integrity checkpoints. PMID: 17209125
亞細(xì)胞定位：

Nucleus.
蛋白家族：

Protein kinase superfamily, Ser/Thr protein kinase family, WEE1 subfamily
組織特異性：

Expressed in shoot apex, vasculatures tissues of roots and leaves, and developing flowers.
數(shù)據(jù)庫鏈接：

KEGG: ath:AT1G02970

STRING: 3702.AT1G02970.1

UniGene: At.42570