E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
RACK1A; ARCA; At1g18080; T10F20.9; T10O22.6; Receptor for activated C kinase 1A; Guanine nucleotide-binding protein subunit beta-like protein A; WD-40 repeat auxin-dependent protein ARCA
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產品提供形式:
Lyophilized powder
Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet :
Please contact us to get it.
產品評價
靶點詳情
功能:
Major component of the RACK1 regulatory proteins that play a role in multiple signal transduction pathways. Involved in multiple hormone responses and developmental processes. MAPK cascade scaffolding protein involved in the protease IV and ArgC signaling pathway but not the flg22 pathway.
基因功能參考文獻:
the genes responsible for two additional suppressor mutants of acl5-1 designated sac53-d and sac56-d encode a receptor for activated C kinase 1 (RACK1) homolog and a ribosomal protein L4 (RPL4), respectively PMID: 25625317
In total 97 proteins from dehydration, salt stress, ribosomal and photosynthesis pathways are found to potentially interact with RACK1A. PMID: 23435172
RACK1 ensures the accumulation and processing of some miRNA precursors, directly interacts with SERRATE and is part of an AGO1 complex PMID: 23941160
RACK1A protein can form complexes with the gamma subunit of the signal transducing heterotrimeric G protein. PMID: 22068106
RACK1 may be required for normal production of 60S and 80S ribosomes and that its action in these processes may be regulated by abscissic acid. [RACK1A] PMID: 21098678
RACK1A (At1g18080) is involved in multiple signal transduction pathways. PMID: 16829549
RACK1A (At1g18080) is positively regulated by other RACK1 genes. Loss of function causes defect in plant development. PMID: 18947417
RACK1 genes act redundantly to negatively regulate abscisic acid responses during seed germination and early seedling development. PMID: 19584117
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亞細胞定位:
Cytoplasm. Nucleus. Note=Detected in the cytoplasm and nucleus when interacting with NUDT7.
蛋白家族:
WD repeat G protein beta family, Ribosomal protein RACK1 subfamily