TRPM8 Recombinant Monoclonal Antibody

說明書

中文名稱：

TRPM8重組抗體
貨號：

CSB-RA186773A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA186773A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA186773A0HU TRPM8重組單克隆抗體是針對瞬時受體電位通道M8亞型（TRPM8）開發的高特異性科研工具。該抗體采用重組技術制備，具有嚴格的批次一致性和低交叉反應性，經ELISA和免疫組化（IHC）實驗驗證，在IHC應用中推薦使用1:50-1:200的稀釋比例，能清晰定位目標蛋白在組織切片中的表達分布。TRPM8作為溫度敏感型離子通道，主要參與冷覺感知和溫度調控，其功能與痛覺傳導、炎癥反應及腫瘤微環境調控等過程密切相關。本產品適用于神經生物學、痛覺信號通路和溫度感受機制的基礎研究，可支持石蠟包埋或冰凍切片的組織染色分析，為探究TRPM8在病理生理模型中的表達變化提供可靠檢測手段。通過優化驗證的實驗方案，該抗體在多種樣本中均表現出高靈敏度和低背景信號，滿足科研領域對靶標蛋白精準檢測的需求。
Uniprot No.：

Q7Z2W7
基因名：

TRPM8
別名：

Transient receptor potential cation channel subfamily M member 8 (Long transient receptor potential channel 6) (LTrpC-6) (LTrpC6) (Transient receptor potential p8) (Trp-p8), TRPM8, LTRPC6 TRPP8
反應種屬：

Human
免疫原：

A synthesized peptide derived from human TRPM8
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

2G7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonist menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. In prostate cancer cells, shows strong inward rectification and high calcium selectivity in contrast to its behavior in normal cells which is characterized by outward rectification and poor cationic selectivity. Plays a role in prostate cancer cell migration. Isoform 2 and isoform 3 negatively regulate menthol- and cold-induced channel activity by stabilizing the closed state of the channel.
基因功能參考文獻：
1. Study shows the cloning and characterization of N-terminal truncated TRPM8 isoforms in prostate epithelial cells and tumors. These isoforms exhibit likely 4TM domain structure which is distinct from the 6-TM of the TRP channel archetype. MTRPM8 isoforms act as the ER Ca2+-release channels not only in epidermis but also in prostate epithelial cells. PMID: 29678654
2. The present data suggest a possible theoretical foundation for the anti-inflammatory role of TRPM8 activation, providing an experimental basis that could contribute to the advancement of cooling therapy for trauma patients. PMID: 28332601
3. Study shows that TRPM8 expression is significantly up-regulated in pancreatic cancer. PMID: 29860264
4. Data show evidence of recent local ambient temperature adaptation on the cation channel TRPM8. PMID: 29723195
5. TRPM8 levels may be elevated in basal breast cancers, however, TRPM8 expression appears to be lost in many breast cancer cell lines PMID: 29496495
6. In this study, we identify non-channel cytoplasmic small TRPM8 isoforms as putative regulator of PCa cell death PMID: 27074561
7. Praziquantel is a relatively potent and selective partial agonist of the mammalian and avian cold and menthol receptor TRPM8. PMID: 29054683
8. The TRPM8 transient receptor potential channel. PMID: 28693898
9. our results indicate that enhanced TRPM8 hydrolysis in prostate cancer could present an adaptation mechanism, sustained via bypassing testosterone-induced rapid Ca2+ uptake through TRPM8, thus, diminishing the rates of apoptosis PMID: 28039451
10. Suggest a novel, pore-independent molecular mechanism by which endogenous TRPM8 expression inhibits Rap1 GTPase and thus plays a critical role in the behavior of vascular endothelial cells by inhibiting migration. PMID: 28550110
11. in this study, the glycosylation status of the TRPM8 channel was shown to affect cell proliferation, cell migration, and calcium uptake. TRPM8 expressed in the membrane of the Panc-1 pancreatic tumoral cell line is non-glycosylated, whereas human embryonic kidney cells transfected with human TRPM8 overexpress a glycosylated protein. PMID: 28857015
12. This study demonstrates that TRPM8 (cold receptor) is present in nasal epithelium. When it is activated by moderate cooling at 24 degrees C or menthol, TRPM8 induces the secretion of mucin. This study determines that TRPM8 channels are important regulators of mucin production. Therefore, TRPM8 antagonists could be used for the treatment of refractory rhinitis. PMID: 28767579
13. This study suggests that the TRPM8 sensor is a key determinant of germ cell fate under hypothermic stimulation. PMID: 27317670
14. TRPM8 SNPs are significantly associated with the risk of COPD in the Chinese Han population. PMID: 27789940
15. findings demonstrate that both the eTRPM8 expression level and the modalities of cold-stimulation serve as important determinants of the keratinocyte fate and act via fine tuning of [ATP] and [O2*] PMID: 27014839
16. TRPM8 activation is likely to occur when cigarettes contain more than 50 micrograms of menthol. PMID: 28238800
17. This review outlines our current understanding on the role of TRPM8 in occurrence and development of different kinds of tumor and also includes discussion about the regulation of TRPM8 during carcinogenesis as well as therapeutic potential of targeting TRPM8 in tumor, which may be utilized for a potential pharmacological use as a target for anti-cancer therapy.[review] PMID: 26803314
18. The effects of specific agents on TRPV1 and TRPM8 channels are intricately interrelated. PMID: 26645885
19. The data of this study provided evidence for decreased activity and expression level of TRPM8 in the presence of methylglyoxal. PMID: 26629685
20. Study used a combination of molecular modeling and experimental techniques to examine the structure of the TRPM8 transmembrane and pore helix region including the conducting conformation of the selectivity filter PMID: 26536261
21. Translational and clinical investigation to exploit TRPM8 as a molecular biomarker and therapeutic target is expected to make a positive impact on precision medicine in pancreatic cancer and other malignant diseases. PMID: 27038374
22. Several TRPM8 variants appeared to be poorly expressed in transfected HEK293 cells compared with non-mutated TRPM8. PMID: 26330615
23. The GC variant of the TRPM8: c.750G > C (rs11562975) polymorphism is associated with cold-induced airway hyperresponsiveness in patients with bronchial asthma. PMID: 26272603
24. we found that the androgen-mediated TRPM8 channel activity is negatively regulated by the androgen receptor protein PMID: 25980497
25. TRPM8 underlines the important physiologic role of the nose in temperature regulation, both in patients with allergy and those without allergy. Isometric contraction studies demonstrate the role of TRPM8 in regulating nasal patency and airway resistance. PMID: 26163239
26. These results are the first to show that menthol directly binds to the TRPM8 sensing domain. PMID: 26653082
27. Genetic polymorphisms in TRPM8 genes modify individual susceptibility to metabolic syndrome in a Turkish population. PMID: 25967713
28. developed a new functional knockout mouse strain by deleting the pore domain of TRPM8 and demonstrated that eTRPM8 knockout impairs adaptation of the epidermis to low temperatures PMID: 26080404
29. Antibodies against TRPM8 are not found in scleroderma patient sera, suggesting that the abnormal cold sensitivity and associated abnormal vascular reactivity in scleroderma patients is not the result of an immune process targeting TRPM8. PMID: 26242276
30. TRPM8 and TRPA1 assays were inhibited by probenecid. PMID: 24975826
31. Polymorphisms L250L and S419N correlate with anthropometric parameters (body mass index and waist and hip circumferences). PMID: 25348565
32. Results suggests that the aberrantly expressed TRPM8 channel may contribute to pancreatic tumorigenesis by preventing oncogene-induced senescence. PMID: 22555807
33. activation of TRPM8 inhibits UV-B-induced PGE2 production in keratinocytes PMID: 24580132
34. Over-expression of TRPM8 is associated with urothelial carcinoma of bladder. PMID: 25128062
35. The results indicate that the TRPM8 channel is physically associated with testosterone and suggest that, in addition to a genomic role, testosterone plays a role in direct regulation of the TRPM8 channel function. PMID: 25480783
36. This review focus is on TRPV1, TRPA1, and TRPM8 channels in inflammation, energy redirection, and water retention and their role in chronic inflammatory diseases. [review] PMID: 24871046
37. Report novel TRP channel-associated factors that modulate TRPM8 activity. PMID: 25559186
38. Identified two regions within the initial N terminus of TRPM8 that contribute differently to channel activity and proper folding and assembly. Deletion or substitution of the first 40 residues yielded channels with augmented responses to cold and menthol. PMID: 24917670
39. Data indicate that transient receptor potential melastatin 8 (TRPM8) modulates the migration of pancreatic ductal adenocarcinoma (PDA) cells. PMID: 24658318
40. our study provides a mechanistic insight into how TRPM8 promotes the hypoxic growth adaptation of cancer cells via its promotion of RACK1-mediated stabilization of HIF-1alpha PMID: 25065497
41. Data indicate that upregulation of TRPM cation channel TRPM8 promoted epithelial-mesenchymal transition of breast cancer cells via activating c-akt protein/glycogen synthase kinase 3 beta (GSK-3beta) pathway. PMID: 24903376
42. A moderately potent (IC50 = 103 nM), selective TRPM8 antagonist. PMID: 25125580
43. The subjects with homozygous genotype GG of TRPM8 had a lower cold sensation and adequate response to local skin cooling in respect to thermoregulation--decrease in respiratory heat loss and increase in the lipid metabolism PMID: 25272712
44. Human white adipocytes express the cold receptor TRPM8 which activation induces UCP1 expression, mitochondrial activation and heat production. PMID: 24342393
45. The cold receptor transient receptor potential melastatin 8 (TRPM8) undergoes a complex modulation by clinical concentrations of VAs in dorsal root ganglion neurons and HEK-293 cells heterologously expressing TRPM8. PMID: 23943870
46. In uveal melanoma cells, TRPV1, TRPA1 and TRPM8 gene expression were identified. PMID: 24084605
47. Knockout of TRPM8 protein leads to a decrease in morphine-induced cold analgesia. PMID: 23911290
48. our data suggest that TRPM8 induces UCP2 to trigger metabolic transformation, whereas TRPA1 induces autophagy during adverse conditions, and the combination of both genes contributes directly to an invasive phenotype in lung cancer. PMID: 24037916
49. TRPM8 is activated by camphor in a concentration- and temperature-dependent manner. PMID: 23828908
50. Rhinovirus infection causes upregulation of neuronal TRPM8 expression mediated directly by replicating virus. PMID: 24002057
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亞細胞定位：

Cell membrane; Multi-pass membrane protein. Membrane raft. Endoplasmic reticulum membrane.
蛋白家族：

Transient receptor (TC 1.A.4) family, LTrpC subfamily, TRPM8 sub-subfamily
組織特異性：

Expressed in prostate. Also expressed in prostate tumors and in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
數據庫鏈接：

HGNC: 17961

OMIM: 606678

KEGG: hsa:79054

STRING: 9606.ENSP00000323926

UniGene: Hs.366053

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