TPT1 Recombinant Monoclonal Antibody

說明書

中文名稱：

TPT1重組抗體
貨號：

CSB-RA214107A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse liver tissue
All lanes: TPT1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 20, 16 kDa
Observed band size: 25 kDa

IHC image of CSB-RA214107A0HU diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of SY5Y Cells with CSB-RA214107A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunoprecipitating TCTP in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA214107A0HU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA214107A0HU(2μg)+ Hela whole cell lysate(500μg)
Lane 3: Hela whole cell lysate (10μg)
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA214107A0HU TPT1重組單克隆抗體是針對人源TPT1蛋白設計的高特異性科研工具。TPT1（腫瘤蛋白翻譯調控因子1）作為高度保守的胞質蛋白，在細胞增殖調控、凋亡抑制及腫瘤發生中發揮重要作用，其異常表達與多種惡性腫瘤進展相關。本抗體采用重組技術制備，具有優異的批間一致性和低交叉反應性，經嚴格驗證可在多種實驗體系中穩定工作：Western Blot可清晰識別內源性TPT1蛋白，免疫組化能精準定位石蠟切片中的抗原表位，免疫熒光可清晰顯示細胞質內分布特征，免疫沉淀實驗可有效捕獲天然構象的TPT1及其互作蛋白復合物。推薦工作濃度覆蓋WB 1:500-1:5000、IHC 1:50-1:200、IF 1:20-1:200、IP 1:200-1:1000，適用于癌癥機制研究、藥物靶點篩選、細胞周期調控分析等基礎科研領域，特別在腫瘤細胞模型構建、蛋白相互作用網絡解析及分子病理學研究中展現優異性能，為探索TPT1在腫瘤微環境中的生物學功能提供可靠檢測工具。
Uniprot No.：

P13693
基因名：

TPT1
別名：

Translationally-controlled tumor protein (TCTP) (Fortilin) (Histamine-releasing factor) (HRF) (p23), TPT1
反應種屬：

Human, Mouse
免疫原：

A synthesized peptide derived from human TCTP
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

10A9
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200
IP	1:200-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Involved in calcium binding and microtubule stabilization.
基因功能參考文獻：
1. Study findings suggested that TCTP promotes colorectal cancer metastasis through regulating the behaviors of HMGB1 and the downstream activation of the NF-kappaB signaling pathway. PMID: 30066846
2. The biological effects of Na,K-ATPase and TCTP interaction are reviewed. PMID: 29867020
3. The cells knocked down for TCTP show diminished invasiveness and migration under TGF-beta1 treatment. TGF-beta1 dependent TCTP expression is required for epithelial to mesenchymal transition in cells. PMID: 28958626
4. that TPT1 knockdown altered the BECN1 interactome, a representative MTOR-independent pathway, to stimulate autophagosome formation PMID: 28409693
5. Transfection with TCTP-Lipid modified antisense oligonucleotides led to rapid and prolonged internalization via macropinocytosis, TCTP downregulation and significant decreased cell viability in in castration-resistant prostate cancer cells. PMID: 28472637
6. extracellular TCTP has a role as a promoter of colorectal cancer progression and liver metastases via Cdc42/JNK/MMP9 activation PMID: 27367023
7. The structure of human HRF (hHRF) was solved in 2 new crystal forms, one at the highest resolution (1.4A) yet reported. The high resolution hHRF structure reveals a disulphide-linked dimer, in which the 2 molecules are closely associated and provides a model for the role of HRF in mast cell activation. PMID: 29216544
8. analyzed the interactome of TCTP in HeLa cells by coimmunoprecipitation (IP) and mass spectrometry (MS). Results not only reveal a large number of TCTP-associated proteins that possess pleiotropic functions, but also provide novel insights into the molecular mechanisms of TCTP in tumorigenesis PMID: 27607350
9. Fortilin directly interacts with the cytoplasmic domain of IRE1alpha, inhibits both kinase and endoribonuclease (RNase) activities of this stress sensor, and protects cells against apoptotic cell death at both cellular and whole animal levels. PMID: 28550308
10. These findings provide novel data on the role and regulation of TCTP in skeletal muscle in vivo. PMID: 27813490
11. TCTP may thus contribute to the development of anti-cancer drug resistance. PMID: 28143584
12. The findings suggest that the synergistic actions of Histamine Releasing Factor and Histamine Releasing Factor-reactive IgE may play important roles in the pathogenesis of Chronic Urticaria. PMID: 28219065
13. Downregulation of the expression of TCTP inhibits the migration and invasion of glioma cells. PMID: 26328748
14. These data may suggest an involvement of translationally controlled tumor protein in normal physiology and carcinogenesis. PMID: 26425551
15. High HRF expression is associated with non-Hodgkin lymphomas. PMID: 25363345
16. Results demonstrate that TCTP mRNA translation is regulated by signalling through the PI3-K/Akt/mTORC1 pathway in neoplasms. PMID: 25936523
17. High phospho-TCTP expression levels are associated with aggressive breast cancer. PMID: 25779659
18. TCTP binds to hemin and functions as a "buffer protein", controlling cellular homeostasis. PMID: 25396429
19. analysis of how TCTP-PTD penetrates A549 human lung cancer cell membranes and promotes protein internalization PMID: 25423047
20. Data indicate that Tanshinone IIA (Tan-IIA)T upregulated the protein expression of CHOP and Bax, but downregulated the protein expression of BiP, TCTP, Mcl-1 and Bcl-xL. PMID: 25270224
21. Targeting Hsp27 interaction with TCTP, a partner protein identified in a genetic screen, increases therapy sensitivity, and could decrease or abolish toxicity to normal cells. PMID: 25277244
22. TCTP is functionally implicated in the progression of NF1-associated tumors. PMID: 25092287
23. osteoclastogenic activity of translationally-controlled tumor protein (TCTP) with reciprocal repression of p21 PMID: 25263704
24. Interaction of translationally controlled tumor protein with Apaf-1 is involved in the development of chemoresistance in HeLa cells. PMID: 24606760
25. High TCTP expression is associated with glioma. PMID: 24311645
26. Plasmodium falciparum TCTP showed a lower B cell proliferative effect than human TCTP. PMID: 24465583
27. Cofilin-1 expression positively correlates with the tumor grade, and p23 expression correlates with the endoplasmic reticulum positive status. PMID: 24106833
28. TCTP is a novel mediator of endothelial prosurvival and growth in patients with pulmonary arterial hypertension. PMID: 24657995
29. observations indicate that TCTP enhances Pim-3 stability to simultaneously promote and prevent cell-cycle progression and apoptosis, respectively PMID: 24165482
30. TCTP may have a role in prostate cancer development PMID: 23894469
31. Data indicate that translationally controlled tumor protein (TCTP) expression in glioma was an independent prognostic factor, which might be a potential diagnostic and therapeutic target of glioma. PMID: 23749504
32. TCTP binds to the beta domain of VHL through competition with HIF1alpha, which promotes VHL degradation by the ubiquitin-proteasome system and HIF1alpha stability. PMID: 23387829
33. human miR-27b regulates the expression of the TCTP tumor protein, and circulating miR-27b may be useful as a biomarker for oral cancer research. PMID: 22902387
34. Demonstrate the presence of TCTP in human cornea, and suggest a potential role in the pathogenesis of herpes virus keratitis. PMID: 22853445
35. Results imply that TCTP might be a mediator of PRL-3-promoted proliferation, migration and invasion of human colon cancer cells. PMID: 22340241
36. Data suggest that TCTP may play a critical role in maintaining genomic integrity in response to DNA-damaging agents. PMID: 22451927
37. CHD1L/TCTP/Cdc25C/Cdk1 molecular pathway causes the malignant transformation of hepatocytes with the phenotypes of accelerated mitotic progression and the production of aneuploidy PMID: 21953552
38. The TCTP is a survival factor that protects cancer cells from oxidative stress-induced cell-death. PMID: 21801721
39. These results identify TCTP-bearing nanovesicles as a novel component of the paracrine apoptotic program of potential importance in vascular repair. PMID: 20966960
40. The Chfr-TCTP interaction was stable throughout the cell cycle, but it could be diminished by the complete depolymerization of the microtubules. PMID: 18504434
41. Knockdown of TCTP inhibited proliferation, migration, and invasion activities of LoVo cells in vitro and in vivo. PMID: 19621893
42. Data suggest a kind of feedback regulation between TCTP and VDR to regulate a variety of (Ca(2+) dependent) cellular effects and in this way further underscore the physiological relevance of the observed physical interaction beteen TCTP and VDR. PMID: 19815065
43. The mRNA of the translationally controlled tumor protein P23/TCTP is a highly structured RNA, which activates the dsRNA-dependent protein kinase PKR (TCTP PROTEIN) PMID: 11991642
44. Physical and functional interaction between myeloid cell leukemia 1 protein (MCL1) and this protein PMID: 12149273
45. Human TCTP was identified as a putative Plk-interacting clone by a two-hybrid screen. Plk phosphorylates TCTP on two serine residues in vitro and cofractionates with the majority of kinase activity toward TCTP in mitotic cell lysates. PMID: 12167714
46. genetic regulation in tumor reversion PMID: 12399545
47. results do not support the hypothesis that IgE(mn+) induces a late allergic reaction (LAR), but do not exclude the alternative hypothesis that HRFs are released during a LAR and contribute to asthma severity PMID: 12487227
48. Patients with Multiple drug allergy syndrome and more than 1/3 with hypersensitivity to a single antibacterial drug showed the presence of circulating histamine-releasing factors. Such factors might play a role in drug-induced adverse reactions. PMID: 12876410
49. HRF p23 can stimulate nonimmune epithelium such as bronchial epithelium PMID: 12948934
50. Human recombinant histamine-releasing factor can partially inhibit the secretion of IL-2 and IL-13 protein in a dose-dependent manner from both purified peripheral blood T cells and Jurkat T cells. PMID: 14500674
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亞細胞定位：

Cytoplasm.
蛋白家族：

TCTP family
組織特異性：

Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC
數據庫鏈接：

HGNC: 12022

OMIM: 600763

KEGG: hsa:7178

STRING: 9606.ENSP00000431872

UniGene: Hs.374596

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