TOMM22 Recombinant Monoclonal Antibody

說明書

中文名稱：

TOMM22重組抗體
貨號：

CSB-RA277640A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, L02 whole cell lysate, HepF2 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
All lanes: TOMM22 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 18 kDa

IHC image of CSB-RA277640A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

產品描述：

CSB-RA277640A0HU TOMM22重組單克隆抗體是針對線粒體外膜轉位酶復合體22（TOMM22）靶點開發的高特異性科研工具。該抗體識別的TOMM22蛋白是線粒體外膜轉位酶（TOM）復合物的核心組分，在介導核編碼蛋白跨線粒體膜運輸過程中起關鍵作用，其功能異常與細胞能量代謝失調及程序性死亡調控密切相關。經嚴格驗證，本產品在Western Blot實驗中可檢測到單一清晰目標條帶，免疫組化實驗顯示其在線粒體膜結構的特異性定位，推薦使用稀釋度為WB 1:500-1:5000、IHC 1:50-1:200，同時在ELISA平臺中展現優異抗原結合能力。適用于研究線粒體蛋白質轉運機制、細胞能量代謝動態平衡及凋亡信號通路調控等基礎生命科學領域，可為線粒體相關疾病模型的分子機制探索、藥物靶點篩選提供可靠檢測支持。該重組單克隆抗體具有批次穩定性高、交叉反應性低的特點，滿足細胞生物學、分子病理學等研究中對線粒體功能檢測的技術需求。
Uniprot No.：

Q9NS69
基因名：

TOMM22
別名：

Mitochondrial import receptor subunit TOM22 homolog (hTom22) (1C9-2) (Translocase of outer membrane 22 kDa subunit homolog), TOMM22, TOM22
反應種屬：

Human
免疫原：

A synthesized peptide derived from human TOMM22
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

7C11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
基因功能參考文獻：
1. A directed proteomic approach discovered the novel interaction of BKCa with Tom22, a component of the mitochondrion outer membrane import system, and the adenine nucleotide translocator (ANT). PMID: 27592226
2. Tom22 is essential for metabolic conversion, as its knockdown by small interfering RNA (siRNA) completely ablated progesterone conversion in both steroidogenic mouse Leydig MA-10 and human adrenal NCI cells. PMID: 26787839
3. Blockade of mitochondrial protein import triggers the recruitment of PARK2, by PINK1, to the TOMM machinery. PMID: 24149440
4. Studies indicate that PUMA and TOMM22 as potential targets for miRNA-BART viral transcripts. PMID: 22178394
5. The interaction with the cytosolic domain of Tom22 helps Bax to acquire a conformation able to interact with the outer mitochondrial membrane. PMID: 22198199
6. the cytosolic domains of Tom20 and Tom22 function to maintain their substrate preproteins unfolded and prevent them from aggregating on the mitochondrial surface PMID: 14699115
7. We have identified TOM22, a component of the translocase of the outer mitochondrial membrane (TOM), as a mitochondrial receptor of Bax. PMID: 17096026
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亞細胞定位：

Mitochondrion outer membrane; Single-pass membrane protein.
蛋白家族：

Tom22 family
組織特異性：

Ubiquitous.
數據庫鏈接：

HGNC: 18002

OMIM: 607046

KEGG: hsa:56993

STRING: 9606.ENSP00000216034

UniGene: Hs.595072

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