TLR5 Recombinant Monoclonal Antibody

說明書

中文名稱：

TLR5重組抗體
貨號：

CSB-RA545516A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: THP-1 whole cell lysate, RAW264.7 whole cell lysate, U-251 whole cell lysate, K562 whole cell lysate
All lanes: TLR5 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 98 kDa
Observed band size: 98 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

TLR5 Recombinant Monoclonal Antibody（Code: CSB-RA545516A0HU）是一款針對人Toll樣受體5（TLR5）開發的高特異性抗體。TLR5是先天免疫系統中重要的模式識別受體，主要識別細菌鞭毛蛋白并激活NF-κB等信號通路，在宿主防御、炎癥調控及免疫穩態維持中發揮關鍵作用。本抗體經ELISA和Western Blot（WB）嚴格驗證，在WB實驗中展現優異性能，推薦使用稀釋度為1:500至1:5000，能夠有效識別天然及重組TLR5蛋白，實驗結果顯示其特異性結合能力穩定，背景信號低。該產品適用于研究TLR5在病原體識別、免疫細胞活化以及炎癥相關疾病（如腸道炎癥、感染性疾?。┲械姆肿訖C制，也可用于探索TLR5與腫瘤微環境調控或自身免疫性疾病關聯性的基礎研究。作為科研工具，該抗體為體外實驗（如免疫印跡分析、蛋白互作研究）及免疫檢測體系的建立提供了可靠支持，但不適用于臨床診斷或治療用途。
Uniprot No.：

O60602
基因名：

TLR5
別名：

Toll-like receptor 5 (Toll/interleukin-1 receptor-like protein 3), TLR5, TIL3
反應種屬：

Human
免疫原：

A synthesized peptide derived from human TLR5
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

10D8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life.
基因功能參考文獻：
1. SNPs associated with genetically determined high activity of TLR5 among patients with CD and genetically determined high IL-12 and IL-18 levels among patients with UC. PMID: 28139755
2. results suggest that Hsp90 inhibitors suppress TLR5 surface expression and activation of NF-kappaB in THP-1 cells in response to TLR5 ligand, and these inhibitory effects may be associated with the possible mechanisms by which Hsp90 inhibitors suppress myeloid leukemia. PMID: 29651431
3. this study signifies that TLR5 adaptor molecules are necessary for the proper production of cytokines, chemokines and pro-labour mediators after TLR ligation PMID: 28844021
4. the newly found long TLR5 transcripts may be involved in the negative regulation of TLR5 expression and function. PMID: 28948171
5. It was observed that the TLR5 polymorphisms rs5744168, rs2072493, and rs5744174 are less frequent in Indian Tamils, suggesting that the TLR5 gene remains conserved, which may be due to the genetic selection pressure to withstand prevailing endemic infectious diseases. We observed that these polymorphisms also failed to confer significant risk to develop chronic H. pylori infections and its associated clinical phenotypes PMID: 28742407
6. we identified significant interactions between TLR5 rs1640827, rs17163737 and Helicobacter pylori infection. This results provide valuable insights into the TLR5 and Helicobacter pylori infection involved in gastric carcinogenesis, and this may have important implications in personalized prevention of GC. PMID: 28404962
7. TLR 5, 7, and 9 expression patterns differed between HPV-positive and -negative oropharyngeal squamous cell carcinoma patients. In HPV-positive tumors the expression of TLR 5 and 7 correlated with tumor recurrence. PMID: 28856441
8. These results suggest that HMGB1-modulated TLR5 signaling is responsible for pain hypersensitivity. PMID: 27760316
9. data show that TLR-5 and TLR-9 are susceptible genes to lupus nephritis (LN) and that their expression is dysregulated in LN patients' kidneys, supporting a role of these mediators in the pathogenesis of LN. PMID: 28763101
10. These results indicate that in Chinese genetic variation of TLR5 may be not a determinant of susceptibility to hepatitis B virus-related diseases but may play a role in development of hepatitis B virus-related severe liver diseases. PMID: 28543911
11. Distinctive Recognition of Flagellin by Human and Mouse Toll-Like Receptor 5 PMID: 27391968
12. This study independently confirms the association of TLR5 c.1174C>T with protection against death in melioidosis, identifies lower bacteremia, IL-10 and TNF-alpha production in carriers of the variant with melioidosis. PMID: 28475641
13. Study demonstrated that toll-like receptor 5 expression and functional activity as measured by interleukin 6 are modulated by hormones PMID: 27651177
14. findings suggest that TLR5 is functionally expressed in the SG and responds to its cognate ligand flagellin PMID: 27665969
15. that TLR5 is involved in the pathogenesis and dissemination of esophageal adenocarcinoma through as-yet-uncharacterized mechanisms PMID: 27392931
16. Ligands for TLR1/2 or TLR5 may provide critical stimuli able to sustain the growth and the malignant phenotype of MCL cells. PMID: 27123851
17. The distribution of the TLR 5 genotypes did not differ significantly between bronchopulmonary dysplasia patients and controls. PMID: 27457283
18. Both TLR 5 and 7 are expressed in salivary adenoid cystic carcinoma on the cell membranes as well as in cytoplasm. PMID: 26888781
19. The results of this study demonstrated that TLR5 rs5744174 polymorphism may have no impact on the stroke risk, gene expression and inflammatory cytokines, but may influence the HDL-C serum level of IS patients in Chinese Han population. PMID: 27262705
20. Letter: TLR5 mRNA was not altered in peripheral mononuclear cells from patients with ankylosing spondylitis or rheumatoid arthritis. PMID: 26996117
21. These results indicate that in response to Pseudomonas aeruginosa or flagellin, EGFR associates with and tyrosine phosphorylates MUC1-CT in primary normal human bronchial epithelial cells, leading to increased MUC1-CT association with TLR5 PMID: 26645913
22. Study elucidated the relationship between TLR5 and caveolin-1 at the transcriptional and translational levels using human cells, results suggest that caveolin-1 is a crucial regulator for maintaining and controlling TLR5 expression. PMID: 26615831
23. Study showed that MyD88 Toll/interleukin-1 receptor (TIR) domain interacted with TLR5TIR but not with TLR6TIR. The solubility of both TLR5TIR and TLR6TIR were influenced by its binding partner MyD88TIR. Moreover, TLR5TIR exhibited increased solubility. PMID: 26548862
24. TLR-5 was more abundant in OSCC than in CSCC samples. PMID: 25047824
25. genetic polymorphism is not associated with duodenal ulcer PMID: 25995217
26. p53-dependent amplification of transcriptional response to TLR5 activation required expression of NFkappaB subunit p65 and was mediated by several molecular mechanisms PMID: 26220208
27. TLR5 variants R392X and N592S showed significant association with UC patients but TLR 1, 2, 3, 6 variants did not show any association. PMID: 25789623
28. Studies indicate that toll-like receptor 5 (TLR5) specifically interacts with flagellin, and is useful as an analytical recognition element for low concentrations of flagellin. PMID: 24881544
29. review of recent research on the interactions between TLR5, adaptive immunity and the microbiota; discussion of how variation in the TLR5 gene may alter these interactions to impact host inflammatory phenotypes in humans PMID: 25284610
30. Establishing bacterial flagellin-recognizing toll-like receptor 5 (TLR5) as a novel link between gut microbiota composition, adipose tissue inflammation, and obesity. PMID: 25611816
31. Flagellin activated TLR5 protein in non-small cell lung cancer cell lines and initiate downstream signaling pathways. PMID: 25603867
32. mediates cytokine production in response to the Toxoplasma gondii-derived profilin PMID: 24861338
33. Association of TLR5 Gene Polymorphisms in Ulcerative Colitis Patients of North India and Their Role in Cytokine Homeostasis. PMID: 25789623
34. Study demonstrates that TLR5 recognition of commensal microbiota regulates systemic tumor-promoting inflammation and, subsequently, extramucosal malignant progression. PMID: 25533336
35. A family was heterozygous for risk variants of the genes encoding NOD2 and TLR5 and homozygous carriers of PTPN2 risk alleles in Crohn disease. PMID: 24901824
36. Genetic polymorphisms in TLR5 might be associated with decreased susceptibility of Graves' disease in Chinese Cantonese females. PMID: 24830583
37. results reveal broad expression patterns of TLR5 and TLR7 in the lung and that the expression is decreased in severe asthma. PMID: 24447081
38. TLR5 activation by flagellin may elicit chemoresistance in Multiple myeloma patients who have suffered from recurrent bacterial infections. PMID: 24709011
39. Sle1 transgene perturbs the action of peripheral tolerance checkpoints operative on antinuclear antigen B cells in both the antibody-forming cell and germinal center pathways in a cell autonomous fashion. PMID: 19828626
40. TLR5(rs5744105)variant was associated with altered immune responses in very low birth weight infants (elevated WBC counts)during bacterial infections. PMID: 23867959
41. Frequent SNP's in the flagellin receptor TLR5 and the TLR downstream effector molecules MyD88 and TIRAP that are associated with altered survival in a large cohort of Caucasian patients with colorectal cancer PMID: 24154872
42. Low expression of TLR5 was associated with non-small cell lung cancer. PMID: 24549739
43. Authors suggest that TLR5 is involved in host defense against Aspergillus fumigatus, and that the recipient TLR5-Stop SNP represents a risk factor for the development of invasive aspergillosis after allogeneic stem cell transplantation. PMID: 23862689
44. suggest a role for abnormal expression of TLR5 in the pathogenesis of esophageal adenocarcinoma and suggest importance of altered microbiome in the pathogenesis of complications of Barrett's esophagus PMID: 24221343
45. exposure of human T cells to flagellin leads to a transient TLR5-induced increase in the sensitivity of these cells to subsequent TCR activation. PMID: 24128895
46. TLR5 signaling enhances the proliferation of human allogeneic CD40-activated B cell induced CD4hiCD25+ regulatory T cells. PMID: 23844139
47. These results indicate that polyethylenimine is a new TLR5 agonist with potential application in offering protection for patients receiving radiotherapy or in radiation-related accidents. PMID: 23104900
48. A non-synonymous coding variant (L616F) in the TLR5 gene is potentially associated with Crohn's disease and influences responses to bacterial flagellin. PMID: 23593463
49. Our data demonstrate a novel role for TLR-5 in rheumatoid arthritis angiogenesis; thus, TLR-5 may be a promising new target for its treatment. PMID: 23666857
50. Data indicate the biological activity of a TLR5:flagellin complex with a 2:2 stoichiometry. PMID: 23624387
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相關疾?。?/div>
Systemic lupus erythematosus 1 (SLEB1)
亞細胞定位：

Cell membrane; Single-pass type I membrane protein.
蛋白家族：

Toll-like receptor family
組織特異性：

Highly expressed on the basolateral surface of intestinal epithelia. Expressed also in other cells such as lung epithelial cells.
數據庫鏈接：

HGNC: 11851

OMIM: 109100

KEGG: hsa:7100

STRING: 9606.ENSP00000340089

UniGene: Hs.604542

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