S1PR1 Recombinant Monoclonal Antibody

說明書

中文名稱：

S1PR1重組抗體
貨號：

CSB-RA923827A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, U251 whole cell lysate
All lanes: EDG1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA923827A0HU S1PR1重組單克隆抗體是針對鞘氨醇-1-磷酸受體1（S1PR1）研發的高特異性科研工具。該靶點作為G蛋白偶聯受體家族成員，通過介導鞘氨醇-1-磷酸信號參與細胞遷移調控、血管新生及免疫穩態維持，在炎癥反應、腫瘤微環境調控等病理生理過程中發揮重要作用。本產品采用重組表達技術制備，經ELISA和Western Blot嚴格驗證，在1:500至1:5000稀釋范圍內展現出優異的檢測靈敏度和靶標特異性，能夠清晰識別內源性表達的S1PR1蛋白。其穩定可靠的性能特別適用于研究受體介導的信號轉導機制、免疫細胞趨化運動實驗、血管生成模型構建等基礎研究領域，同時可為相關藥物靶點的分子機制探索提供技術支持。該抗體通過標準化生產工藝實現嚴格的批次間一致性，確保實驗數據的可重復性，滿足科研工作中對蛋白定位、表達水平分析等多種實驗需求。
Uniprot No.：

P21453
基因名：

S1PR1
別名：

S1PR1; CHEDG1; EDG1; Sphingosine 1-phosphate receptor 1; S1P receptor 1; S1P1; Endothelial differentiation G-protein coupled receptor 1; Sphingosine 1-phosphate receptor Edg-1; S1P receptor Edg-1; CD antigen CD363
反應種屬：

Human
免疫原：

A synthesized peptide derived from human EDG1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

4F2
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis. Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury.
基因功能參考文獻：
1. Overexpression of S1PR1 promoted subcutaneous xenograft growth and pulmonary metastases and enhanced expression of epithelial-to-mesenchymal transition markers. PMID: 30453284
2. Knockdown of BATF3 in HL cell lines revealed that BATF3 contributed to the transcriptional programme of primary HRS cells, including the upregulation of S1PR1. PMID: 28878352
3. Data suggest that sphingosine-1-phosphate receptor 1 (S1P1) exhibits a inhibitory effect on kidney ischemia/reperfusion injury-inducedepithelial to mesenchymal transition (EMT) in the kidney by affecting the PI3K/Akt pathway. PMID: 29901713
4. Extracellular alpha-synuclein induces sphingosine S1P1R uncoupled from inhibitory G-protein leaving beta-arrestin signal intact. PMID: 28300069
5. These results demonstrated that ApoM protein mass were clearly higher in the NSCLC tissues than in non-small cell lung cancer (NSCLC) tissues. Overexpression of ApoM could promote NSCLC cell proliferation and invasion in vitro and tumor growth in vivo, which might be via upregulating S1PR1 and activating the ERK1/2 and PI3K/AKT signaling pathways. PMID: 29750961
6. We conclude that S1P attenuates the invasion of C643 cells by activating S1P2 and the Rho-ROCK pathway, by decreasing calpain activity, and by decreasing the expression, secretion and activity of MMP2 and, to a lesser extent, MMP9. Our results thus unveil a novel function for the S1P2 receptor in attenuating thyroid cancer cell invasion. PMID: 29734379
7. Elevated S1PR1 expression and STAT3 activation are also observed in human NB cells with acquired resistance to etoposide. We show in vitro and in human NB xenograft models that treatment with FTY720, an FDA-approved drug and antagonist of S1PR1, dramatically sensitizes drug-resistant cells to etoposide. PMID: 28716816
8. this review discusses sphingosine-1-phosphate receptor as a novel therapeutic target in ulcerative colitis PMID: 27494562
9. Astrocytic S1PR controls astrocyte neurotoxicity and monocyte recruitment and activation. PMID: 28167760
10. High S1PR1 expression is associated with anti-neutrophil cytoplasmic antibody-associated vasculitis. PMID: 28206609
11. Low S1PR1 expression is associated with Coxsackievirus B3-induced myocarditis. PMID: 28986246
12. These findings are consistent with a model in which signalling via S1P and S1PR1 are integral components in the response of lymphatic endothelial cells to the stimulus provided by fluid flow. PMID: 27974574
13. S1PR1/3 silencing alters proliferation, adhesion, viability and lateral motility in estrogen receptor-negative MCF-7 and estrogen receptor-positive MDA-MB-231 breast cancer cells. PMID: 28982858
14. Activated S1PR1 signaling regulates the production of cellular adhesion molecules by inhibiting NF- kappaB activation via a beta-arrestin2-dependent manner PMID: 28399143
15. Model recombinant HDL (rHDL) particles formed in vitro with S1P incorporated into the particle initiated the internalization of S1PR1, whereas rHDL without supplemented S1P did not, suggesting that S1P transported in HDL can selectively activate S1PR1. PMID: 27881715
16. Blocking of S1PR activity and inhibition of S1P synthesis led to decreased expression of fibrosis and tissue remodeling-related proteins in primary cultures of orbital fibroblasts derived from patients with GO. PMID: 28492873
17. a lipid molecule repeatedly entered the receptor between the extracellular ends of TM1 and TM7, providing important insights into the pathway of ligand entry into the S1PR1 . PMID: 28370663
18. Mature human thymocytes rely on S1P-R1 to migrate toward S1P. Taken in the context of murine work demonstrating that S1P is required for thymocyte egress to the periphery. PMID: 27056271
19. ApoM-bound sphingosine-1-phosphate regulates adhesion molecule abundance, leukocyte-endothelial adhesion, and endothelial barrier function via sphingosine-1-phosphate receptor1. PMID: 27879252
20. The S1P1,3 activation results in Akt phosphorylation and subsequent activation of eNOS via phosphorylation at serine(1177) and dephosphorylation at threonine(495). PMID: 27282481
21. HDL-associated ApoM is anti-apoptotic by delivering sphingosine 1-phosphate to S1P1 and S1P3 receptors on vascular endothelium. PMID: 28179022
22. The present high prevalence of S1PR1 overexpression warrants the consideration of testicular diffuse large B-cell lymphoma as a distinct disease subtype and suggests the potential of the S1P/S1PR1 axis as a therapeutic target. PMID: 27061580
23. S1PR1 inhibits the apoptosis of human myeloid leukemia cells and promotes their proliferation. PMID: 27572094
24. These results suggest that S1P signaling plays a role in cell proliferation and drug resistance in multiple myeloma, and targeting this pathway will provide a new therapeutic direction for multiple myelomamanagement. PMID: 27785703
25. Sphingosine 1-phosphate-induced IL-8 gene expression is mainly regulated via S1PR(1), and its secretion is regulated through S1PR(2) receptor subtype. PMID: 26321412
26. multiple lines of evidence demonstrate that S1PR1 signaling sets the sensitivity of pDC amplification of IFN responses, thereby blunting pathogenic immune responses PMID: 26787880
27. The results indicate that sphingosine 1-phosphate is involved in the migration of precursor T-cell lymphoblastic leukemia-lymphoma blasts in vitro, which is dependent on S1P1 expression. PMID: 26824863
28. Overexpression of miR148a or inhibition of S1PR1 suppressed SKOV3 cell migration and invasion, while restoration of S1PR1 expression reversed the suppressive effect of miR148a upregulation on SKOV3 cell migration and invasion. PMID: 26004261
29. the ability of ApoM(+)HDL to act as a biased agonist on S1P1 inhibits vascular inflammation, which may partially explain the cardiovascular protective functions of HDL. PMID: 26268607
30. S1P receptor modulation reduces leukocyte migration across the endothelial barrier PMID: 26197437
31. SGPL1 ratio correlated with increased cellular sphingosine-1-phosphate (S1P), and S1P correlated with drug resistance (IC50). PMID: 26493335
32. The role of sphingosine-1-phosphate in the production of immature double-negative thymocytes in experimental and chronic Chagas disease is reported. PMID: 25330249
33. Within lymphoid follicles, the S1P1 distribution pattern is complementary to that of CCR7/CXCR4, with strong immunoreactivity tightly restricted to mantle zone B cells. This may play a role in B-cell migration toward the S1p-enriched follicle center. PMID: 26282174
34. Ponesimod, a selective S1P1 receptor modulator, causes small, dose-dependent QT prolongation. PMID: 25287214
35. rapid reduction of endothelial cell surface expression of S1PR1 subsequent to Y143 phosphorylation is a crucial mechanism of modulating S1PR1 signaling, and hence the endothelial barrier repair function of S1P. PMID: 25588843
36. Sphingosine-1-phosphate promotes extravillous trophoblast cell invasion by activating MEK/ERK/MMP-2 signaling pathways via S1P/S1PR1 axis activation. PMID: 25188412
37. both S1PR1 and S1PR2 play a pivotal role in hyperglycemia-induced EC dysfunction and endothelial injury by reducing and enhancing the production of oxidative stress. PMID: 25673082
38. S1PR1 expression was associated with unfavourable clinicopathological features and the expression of several anti-apoptosis/proliferation-related markers in urothelial carcinoma. PMID: 26238015
39. Data suggest an SNP in S1PR1 (rs41287280) is associated with protection against coronary artery disease; some SNPs in S1PR1 have the effect that endocytosis is resistant to S1PR1 antagonists (fingolimod) or S1PR1 agonists (sphingosine 1-phosphate). PMID: 25293589
40. Activation of the S1PR1-PLC-IP3 R-Ca(2+) -Rac1 pathway governs the low-dose S1P-enhanced endothelial barrier integrity. PMID: 25557733
41. S1P interacts with its receptors on neutrophils, resulting in NADPH oxidase activation by the PI3K-Akt cell signaling pathway and induction of the neutrophil respiratory burst. PMID: 25872153
42. S1P1 is widely expressed across tissues and, when activated, has broad functions in the immune, vascular and nervous systems. [Review] PMID: 25498971
43. Our data demonstrate that neuronal cell death evoked by ceramide is regulated by PARP/PAR/AIF and by S1P receptor signalling. PMID: 24420784
44. Expression of functional S1PR1 is reduced by B cell receptor signaling and increased by inhibition of PIK3CD but not SYK or BTK in chronic lymphocytic leukemia cells. PMID: 25632006
45. The data demonstrates that S1P1 expression is differentially modulated in grey matter and white matter lesions in multiple sclerosis. PMID: 23551178
46. S1PR1 was a target of miR-125b-1-3p in the placenta. Overexpression of S1PR1 could reverse the inhibitory effect of miR-125b-1-3p on the invasion of trophoblast cells. Abnormal expression of miR-125b-1-3p might contribute to the etiology of preeclampsia. PMID: 25251470
47. Study demonstrates a stark contrast between the consequences of S1PR1 signaling in Treg cells in the periphery versus tumors. PMID: 24630990
48. Aberrant S1PR1 expression in colorectal cancer was associated with metachronous liver metastasis and worse survival outcome PMID: 24972972
49. Data show that sphingosine kinase SphK1 and sphingosine-1-phosphate (S1P) receptors S1P1, S1P2, S1P3, and S1P5 were expressed from primary, up to recurrent and secondary glioblastomas, with sphingosine kinase SphK2 levels were highest in primary tumors. PMID: 24903384
50. study demonstrates loss of S1P and sphingosine kinase activity early in AD pathogenesis, and prior to AD diagnosis. Our findings establish a rationale for further exploring S1P receptor pharmacology in the context of AD therapy. PMID: 24456642
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亞細胞定位：

Cell membrane; Multi-pass membrane protein. Endosome. Membrane raft. Note=Recruited to caveolin-enriched plasma membrane microdomains in response to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine. Ligand binding leads to receptor internalization.
蛋白家族：

G-protein coupled receptor 1 family
組織特異性：

Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
數據庫鏈接：

HGNC: 3165

OMIM: 601974

KEGG: hsa:1901

STRING: 9606.ENSP00000305416

UniGene: Hs.154210

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