Phospho-PXN (Y118) Recombinant Monoclonal Antibody

說明書

中文名稱：

磷酸化-PXN (Y118)重組抗體
貨號：

CSB-RA019112A118phHU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in Hela whole cell lysate,A549 whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or EGF)
All lanes Phospho-PXN antibody at 1.43μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68 KDa
Observed band size: 68 KDa
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

Phospho-PXN (Y118) Recombinant Monoclonal Antibody（CSB-RA019112A118phHU）是一種高特異性抗體，靶向樁蛋白（Paxillin）在Y118位點(diǎn)的磷酸化修飾形式。PXN作為細(xì)胞黏著斑的關(guān)鍵支架蛋白，通過整合細(xì)胞外基質(zhì)信號與細(xì)胞內(nèi)肌動蛋白骨架重組，調(diào)控細(xì)胞遷移、增殖及信號轉(zhuǎn)導(dǎo)等過程。其Y118位點(diǎn)的磷酸化修飾與整合素介導(dǎo)的信號通路活化密切相關(guān)，在腫瘤細(xì)胞侵襲、血管生成及胚胎發(fā)育等研究中具有重要價值。本抗體采用重組單克隆技術(shù)生產(chǎn)，經(jīng)ELISA和Western Blot（WB）嚴(yán)格驗(yàn)證，在WB實(shí)驗(yàn)中推薦使用1:500至1:5000的稀釋度，可清晰識別內(nèi)源性磷酸化PXN蛋白。該產(chǎn)品適用于研究細(xì)胞黏附動力學(xué)、腫瘤微環(huán)境調(diào)控、細(xì)胞運(yùn)動相關(guān)信號通路機(jī)制等基礎(chǔ)科研領(lǐng)域，特別在探究酪氨酸激酶信號對細(xì)胞遷移影響的實(shí)驗(yàn)中表現(xiàn)優(yōu)異。通過精準(zhǔn)檢測Y118磷酸化水平，可為細(xì)胞力學(xué)響應(yīng)、癌癥轉(zhuǎn)移機(jī)制及藥物靶點(diǎn)篩選提供可靠工具，僅限科學(xué)研究使用。
Uniprot No.：

P49023
基因名：

PXN
別名：

FLJ16691 antibody; FLJ23042 antibody; Paired box protein Pax 1 antibody; PAX 1 antibody; PAX1 antibody; PAXI_HUMAN antibody; Paxillin alpha antibody; Paxillin antibody; PXN antibody; PXN protein antibody
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from Human Phospho-PXN (Y118)
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

1F11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
基因功能參考文獻(xiàn)：
1. Data suggest that FGFR3 with mutation found in patients with SADDAN (but not FGFR3 with mutation found in patients with TDII) affects cytoskeleton organization in chondrocytes by inducing tyrosine hyperphosphorylation of paxillin. (FGFR3 = fibroblast growth factor receptor 3; SADDAN = Severe Achondroplasia with Developmental Delay and Acanthosis Nigricans; TDII = Thanatophoric Dysplasia type II) PMID: 29242050
2. overexpression of Paxillin significantly decreased tumor volume of colorectal cancer; miR-24 was overexpressed in natural killer cells and inhibited paxillin expression. PMID: 29494963
3. XIST positively regulated PXN levels by sponging miR-137 in vitro and in vivo. Collectively, our study provided the evidence for the cross-talk between XIST, miR-137, and PXN, shedding light on the therapy for non-small cell lung cancer . PMID: 29337100
4. we herein demonstrated that frequent overexpression of PXN in cervical cancer was associated with advanced tumor stage, poor differentiation and metastasis, and poor predictive outcomes PMID: 29318915
5. Kindlin supports platelet GPIIB IIIA activation by interacting with paxillin. PMID: 28954813
6. we collected paraffin specimens from 85 GBM patients and evaluated the expression pattern of paxillin. Notably, we found that discernable paxillin signals were detected in 67 out of 85 samples. Given that leading edge is critical for cancer cell migration, we propose that NA treatment may be developed into a potential therapy for malignant glioma PMID: 28656206
7. Pxn binding to the CD103 cytoplasmic tail triggers alphaEbeta7 integrin outside-in signaling that promotes CD8(+) T-cell migratory behavior and effector functions. PMID: 29021139
8. REVIEW of the functions of paxillin in pathological conditions, particularly in cell migration PMID: 28214467
9. This study showed that the PXN plays as an oncogene in glioma progression and suggests a new potential biotarget for therapy PMID: 27637748
10. The MBNL3 splicing factor promotes hepatocellular carcinoma by increasing paxillin expression through the alternative splicing of lncRNA-PXN-AS1. PMID: 28553938
11. These data suggest that paxillin appears to influence major cell functions in a diverse range of prostate and breast cancer models. The responsiveness of cells to environmental factors such as HGF or BME may be influenced by paxillin status, although this seems to be dependent on cell type PMID: 28739717
12. The role of paxillin in the aging process of skin cells is reviewed. PMID: 27708212
13. interactions between Cat-1 and its binding partner paxillin are necessary to ensure sufficient Akt activation so that cancer cells are able to grow under anchorage-independent conditions PMID: 28100775
14. The present findings demonstrated that the anticancer effect of docetaxel induces the apoptosis of prostate cancer via the suppression of the cofilin1 and paxillin signaling pathways, which will assist in setting a stage for the clinical treatment of prostate cancer. PMID: 27035282
15. Results showed that the positive rate of PXN was significantly higher in the colorectal adenocarcinoma samples and correlated with TNM stage, distant metastasis and recurrence additionally to cetuximab resistance. PMID: 26530439
16. blockade of GD3-mediated growth signaling pathways by siRNAs might be a novel and promising therapeutic strategy against malignant melanomas, provided signaling molecules such as p130Cas and paxillin are significantly expressed in individual cases. PMID: 27068854
17. These findings suggest that PXN expression has potential use as a novel biomarker of laryngeal squamous cell carcinoma patients and may serve as an independent predictive factor for prognosis. PMID: 26464671
18. Mode of Action of Functionally Important Regions in the Intrinsically Disordered Paxillin PMID: 26928467
19. Paxillin was expressed at significantly higher levels in colorectal cancer tissues and might serve as a potential prognostic indicator in patients with colorectal cancer PMID: 26159303
20. these data suggested that miR-145 plays a pivotal role in colon cancer through inhibiting cell proliferation migration and invasion, and miR-145 may serve as a tumor suppressor by targeting paxillin gene. PMID: 25973017
21. In colorectal cancers PXN was positively correlated with Bcl-2, pBcl-2-S87, and MMP2 expression. PXN promotes Bcl-2 phosphorylation at Serine 87 via ERK activation, increases xenograft tumor formation, and associates with poor patient outcome. PMID: 25826088
22. Bcl-2 stabilization by paxillin confers 5-fluorouracil resistance in colorectal cancer. PMID: 25323586
23. Fascin-1 and paxillin were expressed in 58% and 43% of infiltrating duct carcinoma cases. There was a significant correlation between fascin-1 and paxillin expression and tumor grade, clinical stage, lymph-node metastasis grade, and HER2 expression. PMID: 26349603
24. Suggest paxillin up-regulation and phosphorylation as an important mechanism of vascular remodelling underlying pulmonary hypertension. PMID: 25231004
25. During early cell spreading, DLC1 is preferentially localized at the inner/mature adhesions whereas phosphorylated paxillin occupies the outer/nascent focal adhesions. In addition, DLC1 downregulates paxillin turnover PMID: 25448629
26. The expression levels of Wnt5a, p-JNK1 and p-paxillin in tumor tissues were correlated with each other. PMID: 24395444
27. LPS-induced paxillin phosphorylation at Y31 and Y118 was mediated by c-Abl tyrosine kinase PMID: 25795725
28. This chapter outlines recent advances in understanding how paxillin regulates both steroid and growth factor signaling, focusing on the conserved nature of its actions from a frog germ cell to a human cancer cell. PMID: 25182764
29. Study provides evidence that phosphorylation of PXN is required for cisplatin resistance in lung cancer cells. PMID: 24096476
30. High PXN expression is associated with oral cavity squamous cell carcinoma. PMID: 24894864
31. paxillin may promote cell proliferation and inhibit apoptosis in SW480 cells. Paxillin may be a potential metastasis predictor, and an independent prognosis factor of recurrence. PMID: 24451945
32. MEKK2 induces paxillin ubiquitylation in breast cancer cells and this function requires both paxillin LD1 motif and MEKK2 kinase activity. PMID: 25190348
33. Paxillin knockdown increases capillary endothelial cell migration and invasiveness, and thereby enhances microvessel ingrowth, by suppressing NRP2 expression. PMID: 24522185
34. Through HDAC6-dependent regulation of the microtubule cytoskeleton, paxillin regulates both Golgi organelle integrity and polarized cell invasion. PMID: 25070956
35. PXN plays an important role in tumor progression and may be used as a potential prognostic indicator in gastric cancer PMID: 24180516
36. Future studies investigating these hypotheses on TG-2-paxillin relationships are necessary in order to address this fundamental process in cell matrix adhesion signaling. PMID: 24193434
37. The data suggests that mutant PXN variants play a prominent role in mitochondrial dynamics with direct implications on lung cancer progression. PMID: 23792636
38. In aneuploid tumors, EZH2 expression and paxillin expression correlate with more aggressive phenotype of breast cancer. PMID: 24344012
39. ezrin and paxillin may have roles in aggressive tumor features and invasiveness in urothelial bladder tumors PMID: 21868260
40. The paxillin plays vital roles in cell motility through regulation of focal adhesion dynamics. PMID: 22481092
41. Lysophosphatidic acid (LPA) induces both time and dose-dependent tyrosine phosphorylation of paxillin and focal adhesion kinase. PMID: 24061591
42. Paxillin signaling contributed to tumor growth and vasculogenic mimicry of gallbladder carcinomas. PMID: 23588386
43. Mutation of paxillin serine 250 prevents its phosphorylation by SLK in vitro and results in impaired migration in vivo as evidenced by an accumulation of phospho-FAK-Tyr397 and altered FA turnover rates. PMID: 23128389
44. Data indicate that lasp-2 interacts with the focal adhesion proteins vinculin and paxillin. PMID: 23389630
45. overexpression of PXN induced by suppression of miR-137 promotes tumor progression and metastasis and could serve as an independent prognostic indicator in colorectal cancer patients. PMID: 23275153
46. Paxillin is critical for integrating physical cues from the ECM with chemical motility signals by spatially constraining where cells form motile processes, and thereby regulates directional migration. PMID: 23076140
47. Fascin-1, ezrin and paxillin contribute to the malignant progression and are predictors of clinical prognosis in laryngeal squamous cell carcinoma. PMID: 23209815
48. Paxillin is a new regulator protein of pulmonary arterial smooth muscle cell growth. PMID: 22959909
49. phosphorylation of GIT1 on serine 46 by PKD3 represents a molecular switch by which GIT1 localization, paxillin trafficking, and cellular protrusive activity are regulated. PMID: 22893698
50. Findings suggest that the MLK3-JNK-paxillin signaling axis may represent a potential prognostic marker in breast cancer metastasis. PMID: 22700880
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亞細(xì)胞定位：

Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex.
蛋白家族：

Paxillin family
數(shù)據(jù)庫鏈接：

HGNC: 9718

OMIM: 602505

KEGG: hsa:5829

STRING: 9606.ENSP00000228307

UniGene: Hs.446336

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