Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) Recombinant Monoclonal Antibody
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中文名稱：磷酸化-MAPK8/MAPK9/MAPK10 (T183/T183/T221)重組抗體
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貨號：CSB-RA013466A183phHU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in 293 whole cell lysate(treated with EGF or not)
All lanes Phospho-MAPK8/MAPK9/MAPK10 antibody at 1.65μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46,54 KDa
Observed band size: 46,54 KDa -
Immunocytochemistry analysis of CSB-RA013466A183phHU diluted at 1:165 and staining in Hela cells(treated with 100ng/ml EGF for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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其他：
產品詳情
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產品描述：該重組單克隆抗體靶向MAPK8/JNK1、MAPK9/JNK2和MAPK10/JNK3在Thr183/Thr183/Thr221位點的磷酸化修飾，可特異性識別JNK家族活化形式。JNK信號通路在細胞應激響應、增殖調控及凋亡過程中發揮核心作用，其磷酸化激活與神經退行性疾病、腫瘤發生等病理機制密切相關。經嚴格驗證，該抗體在Western Blot中可檢測多種細胞裂解液樣本，呈現單一明確條帶；在免疫組化實驗中能清晰顯示石蠟包埋組織的亞細胞定位信號，且背景干擾低。推薦應用于體外實驗中JNK信號通路的激活狀態檢測，如氧化應激模型構建、藥物靶點篩選或基因功能研究中信號轉導機制解析。采用重組抗體技術生產，確保批次間高一致性和穩定性，適用于需要定量分析的ELISA檢測體系及高分辨率的免疫印跡實驗，為細胞信號轉導研究提供可靠工具。
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Uniprot No.：
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反應種屬：Human
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免疫原：A synthesized peptide derived from Human Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：1A9
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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