Phospho-Histone H3.3 (T3) Recombinant Monoclonal Antibody

說明書

中文名稱：

磷酸化-組蛋白 H3.3 (T3)重組抗體
貨號：

CSB-RA010109A03phHU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in:Hela whole cell lysate,293 whole cell lysate,NIH/3T3 whole cell lysate
All lanes:Phospho-Histone H3 (T3) antibody at 1.41μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 KDa
Observed band size: 16 KDa

Immunocytochemistry analysis of CSB-RA010109A03phHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Overlay histogram showing Hela cells stained with CSB-RA010109A03phHU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA010109A03phHU是一款特異性識別組蛋白H3.3第3位蘇氨酸磷酸化修飾（p-T3）的高靈敏度抗體。該抗體靶向的H3.3-T3磷酸化是染色體濃縮和有絲分裂過程中的關鍵表觀遺傳標記，廣泛參與細胞周期調控、染色質動態重塑及基因組穩定性維持等生物學過程。經嚴格驗證，本抗體適用于多種實驗場景，包括ELISA檢測磷酸化修飾水平、Western Blot分析蛋白表達、免疫細胞化學（ICC）定位亞細胞分布以及流式細胞術（FC）定量細胞群體中磷酸化信號動態變化，推薦工作濃度分別為WB 1:500-1:5000及ICC/FC 1:50-1:500。其重組單克隆特性確保批次間一致性，適用于細胞周期研究、表觀遺傳調控機制解析、腫瘤細胞增殖與分化相關實驗，尤其在同步化細胞模型或藥物干預研究中，可精準檢測有絲分裂期特異性標記，為細胞生物學與分子機制研究提供可靠工具。本產品為科研用途設計，不用于臨床診斷。
Uniprot No.：

P84243
基因名：

H3F3A
別名：

H3 histone family 3A antibody; H3 histone family 3B antibody; H3 histone; family 3B (H3.3B) antibody; H3.3 antibody; H3.3A antibody; H3.3B antibody; H33_HUMAN antibody; H3F3 antibody; H3F3A antibody; H3f3b antibody; Histone H3.3 antibody; Histone H3.3Q antibody; Histone H3.A antibody; Histone H3.B antibody; MGC87782 antibody; MGC87783 antibody
反應種屬：

Human, Mouse
免疫原：

A synthesized peptide
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

28H4
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, ICC, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
ICC	1:50-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
基因功能參考文獻：
1. We conclude that the Clival GCT is genetically defined by somatic mutation in the H3F3A gene, linking it to the GCT of long bones. PMID: 29609578
2. overexpression of H3F3A, encoding H3.3, is associated with lung cancer progression and promotes lung cancer cell migration by activating metastasis-related genes PMID: 27694942
3. we describe the presence of the mutation p.K27M of H3F3A (H3.3K27M) in two tumours of young patients with classical histopathology of ganglioglioma PMID: 27219822
4. H3F3 mutations are sensitive and specific markers of giant cell tumors of the bone and chondroblastomas PMID: 28059095
5. H3F3A is the most frequently mutated giant cell tumor of bone driver gene. H3F3A mutations are not present in atypical giant cell tumor of bone. PMID: 28545165
6. H3F3A mutational testing may be a useful adjunct to differentiate giant cell tumor of bone from giant cell-rich sarcoma. PMID: 28899740
7. Report H3F3A/B mutations in cell tumors of bone, chondroblastomas, and aneurysmal bone cysts. PMID: 28882701
8. Data suggest that H3K9ac (histone 3 lysine 9 acetylation) serves as a substrate for direct binding of the SEC (super elongation complex) to chromatin; at select gene promoters, H3K9ac loss or SEC depletion appears to reduce gene expression. PMID: 28717009
9. We determined the incidence of H3.3 G34 mutations in primary malignant bone tumors as assessed by genotype and H3.3 G34W immunostaining PMID: 28505000
10. The kinase activity of Aurora B on serine 31 of histone H3.3 was biochemically confirmed with nucleosomal substrates in vitro. PMID: 28137420
11. This study showed that heterozygous K27M mutations in H3F3A (n = 4) or HIST1H3B (n = 3) across all primary, contiguous, and metastatic tumor sites in all Diffuse intrinsic pontine glioma. PMID: 26727948
12. Study examined the relationship of K27M mutations in the distinct histone H3 variants (i.e. HIST1H3B and H3F3A) with specific pontine glioma biology PMID: 26399631
13. study found spinal high-grade gliomas in children and adults frequently harbor H3F3A (K27M) mutations PMID: 26231952
14. H3F3A and H3F3B mutation analysis appears to be a highly specific, although less sensitive, diagnostic tool for the distinction of GCTB and chondroblastoma from other giant cell-containing tumors. PMID: 26457357
15. we describe three interesting cases of paediatric glial and glioneuronal tumours harbouring both BRAF V600E and H3F3A K27M mutations. PMID: 25389051
16. our observations further extend the knowledge of H3F3A mutation and its location in pediatric glioblastomas PMID: 25479829
17. The CENP-A/histone H3.3 nucleosome forms an unexpectedly stable structure and allows the binding of the essential centromeric protein, CENP-C, which is ectopically mislocalized in the chromosomes of CENP-A overexpressing tumor cells. PMID: 25408271
18. On the basis of our findings, H3F3A p.Gly34 Trp or p.Gly34 Leu mutations are not a frequent event in CGCL. PMID: 25442495
19. These results suggest that immunohistochemical detection of H3.3 K27M is a sensitive and specific surrogate for the H3F3A K27M mutation and defines a prognostically poor subset of pediatric glioblastomas. PMID: 25200322
20. this study identifies an H3.3K36me3-specific reader and a regulator of intron retention and reveals that BS69 connects histone H3.3K36me3 to regulated RNA splicing, providing significant, important insights into chromatin regulation of pre-mRNA processing. PMID: 25263594
21. The mutually exclusive associations of HDAC1/p300, p300/histone, and HDAC1/histone on chromatin contribute to the dynamic regulation of histone acetylation. PMID: 24722339
22. Loss of H3.3 from pericentromeric heterochromatin upon DAXX or PML depletion suggests that the targeting of H3.3 to PML-NBs is implicated in pericentromeric heterochromatin organization. PMID: 24200965
23. These data suggest that adult brainstem gliomas differ from adult supratentorial gliomas. In particular, histone genes HIST1H3B (K27M) ) mutations are frequent in adult brainstem gliomas. PMID: 24242757
24. H3F3A K27M mutation is associated with thalamic gliomas. PMID: 24285547
25. The results of this study indicate that H3F3A K27M mutant GBMs show decreased H3K27me3 that may be of both diagnostic and biological relevance. PMID: 23414300
26. H3F3A exon 2 mutation analyzed in solid tumors from 1351 South Korean patients PMID: 23758177
27. A remarkable picture of tumor type specificity for histone H3.3 driver alterations emerges, indicating that histone H3.3 residues, mutations and genes have distinct functions. PMID: 24162739
28. Reduced H3K27me3 and/or DNA hypomethylation are the major driving forces of activated gene expression in K27M mutant pediatric high-grade gliomas. PMID: 24183680
29. All reported H3.3 mutations identified in human tumors have been in the H3F3A gene leading to single codon changes within the N-terminal tail of the H3.3 protein. [Review] PMID: 24229707
30. This study suggested that none of H3.3 G34R mutated tumors presented primitive neuroectodermal tumors of central nervous system and pediatric glioblastomas. PMID: 23354654
31. diffuse intrinsic pontine gliomas containing K27M mutation display lower overall amounts of H3 with trimethylated lysine 27(H3K27me3);H3K27M inhibits enzymatic activity of Polycomb repressive complex 2 through interaction with the EZH2 subunit; propose a model where aberrant epigenetic silencing through H3K27M-mediated inhibition of PRC2 activity promotes gliomagenesis PMID: 23539183
32. Low frequency of H3.3 mutations in myelodysplastic syndromes patients. PMID: 23660862
33. indicate that H3.3K27M mutation reprograms epigenetic landscape and gene expression, which may drive tumorigenesis PMID: 23603901
34. H3F3A K27M mutations occur exclusively in pediatric diffuse high-grade astrocytomas PMID: 23429371
35. Somatic mutation of H3F3A, a chromatin remodeling gene, is rare in acute leukemias and non-Hodgkin lymphoma. PMID: 23116151
36. K27M mutation in H3.3 is universally associated with short survival in diffuse intrinsic pontine gliomas, while patients wild-type for H3.3 show improved survival. PMID: 22661320
37. demonstrate that the two H3F3A mutations give rise to glioblastomas in separate anatomic compartments, with differential regulation of transcription factors OLIG1, OLIG2, and FOXG1, possibly reflecting different cellular origins PMID: 23079654
38. recurrent mutations in a regulatory histone in humans; data suggest that defects of the chromatin architecture underlie paediatric and young adult GBM pathogenesis PMID: 22286061
39. discussion of the importance of H3.3 deposition as a salvage pathway to maintain chromatin integrity PMID: 22195966
40. Part of multiple H3.3-specific histone chaperone complexes PMID: 21047901
41. Studies indicate that H3.3 accomplishes a surprising variety of cellular and developmental processes. PMID: 20153629
42. Histone H3 lysine 4 methylation disrupts binding of nucleosome remodeling and deacetylase (NuRD) repressor complex PMID: 11850414
43. analysis of histone posttranslational modifications on H3.1 and H3.3 PMID: 17052464
44. Data reveal that TPA activates transcription of TBX2 through activating MSK1, which leads to an increase in phosphorylated histone H3 and the recruitment of Sp1 to the TBX2 gene. PMID: 19633291
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相關疾?。?/div>
Glioma (GLM)
亞細胞定位：

Nucleus. Chromosome.
蛋白家族：

Histone H3 family
數據庫鏈接：

HGNC: 4764

OMIM: 137800

KEGG: hsa:3020

STRING: 9606.ENSP00000355778

UniGene: Hs.180877

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