PGAM1 Recombinant Monoclonal Antibody
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中文名稱：PGAM1重組抗體
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貨號：CSB-RA935337A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate
All lanes: PGAM1 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 29 kDa
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其他：
產品詳情
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產品描述：CSB-RA935337A0HU PGAM1重組單克隆抗體是針對磷酸甘油酸變位酶1（PGAM1）開發的高特異性科研試劑。該靶標作為糖酵解通路關鍵酶，在細胞能量代謝和生物合成過程中發揮重要作用，其表達異常與多種病理過程密切相關。本產品采用重組技術制備，經嚴格驗證可在Western Blot實驗中穩定識別天然及重組PGAM1蛋白，檢測結果呈現清晰單一條帶，推薦使用稀釋范圍為1:500至1:5000，同時適用于ELISA平臺檢測。其高靈敏度和低背景特性使其成為研究腫瘤代謝重編程、細胞增殖調控以及氧化應激機制的有效工具，特別適用于體外培養細胞模型的蛋白表達分析、代謝通路研究及相關小分子抑制劑的藥效評價等基礎科研領域。該抗體具備優異的批次間穩定性，為科研工作者探索PGAM1在疾病發生發展中的分子機制提供可靠保障。
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Uniprot No.：
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基因名：
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別名：Phosphoglycerate mutase 1 (EC 5.4.2.11) (EC 5.4.2.4) (BPG-dependent PGAM 1) (Phosphoglycerate mutase isozyme B) (PGAM-B), PGAM1, PGAMA
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反應種屬：Human, Mouse, Rat
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免疫原：A synthesized peptide derived from human PGAM1
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：9A5
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.
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基因功能參考文獻：
- High PGAM1 expression is associated with Pancreatic Ductal Adenocarcinoma Metastasis. PMID: 29386088
- conformation and dynamics of the C-terminal region in human phosphoglycerate mutase 1 PMID: 28748916
- When BPGM is knocked out, 1,3-BPG can directly phosphorylate PGAM1. In this case, PGAM1 phosphorylation and activity are decreased, but nevertheless sufficient to maintain normal glycolytic flux and cellular growth rate. PMID: 28805803
- study provided the first evidence revealing a non-metabolic function of PGAM1 in promoting cell migration, and gained new insights into the role of PGAM1 in cancer progression. PMID: 27991922
- Data show that tyrosine 26 phosphorylation enhances the binding of phosphoglycerate mutase 1 (PGAM1) to its substrates through generating electrostatic environment and structural features that are advantageous to the binding. PMID: 28076845
- PGAM1 in promoting homologous recombination repair in tumor cell lines. Suggest potential therapeutic opportunity for PGAM1 inhibitors in combination with PARP inhibitors in cancer treatment. PMID: 28122957
- PGAM1 may be associated with the grade of glioma and be involved in the biological behavior of glioma cells. PGAM1 might be a novel therapeutic target in glioma. PMID: 27572934
- PGAM1 correlates with spermatogenic dysfunction and affects the function of cell proliferation, apoptosis and migration. PMID: 25701843
- Our finding showed that PGAM1 might serve as a promising therapeutic target for UBC. PMID: 26655504
- PGAM1 is highly expressed in clear cell renal cell carcinoma and correlated with clinicalpathological features, which may contribute to tumor formation and progression. PMID: 26464696
- PGAM is acetylated at lysines 100/106/113/138 in its central region, and a member of the Sirtuin family (class III deacetylase), SIRT2, is responsible for its deacetylation. PMID: 25195573
- Tyrosine26 phosphorylation represents an additional acute mechanism underlying phosphoglycerate mutase 1 upregulation. PMID: 23653202
- Phosphoglycerate mutase 1 (PGAM1) contributes to biosynthesis regulation by controlling intracellular levels of its substrate, 3-phosphoglycerate (3-PG), and product, 2-phosphoglycerate (2-PG). PMID: 23153533
- PGAM1 deacetylation and activity are directly controlled by Sirt1. PMID: 22157007
- histidine-phosphorylated PGAM1 correlated with expression of PKM2 in cancer cell lines; decreased pyruvate kinase activity in PKM2-expressing cells allows PEP-dependent histidine phosphorylation of PGAM1 and may provide an alternate glycolytic pathway PMID: 20847263
- Our studies suggested that PGAM1 plays an important role in hepatocarcinogenesis PMID: 20403181
- Results identified phosphoglycerate mutase 1 that showed elevated levels of protein carbonyls in inferior parietal lobule (IPL) from subjects with early stage-Alzheimer's disease. PMID: 19686046
- Cloning, purification, crystallization and preliminary crystallographic analysis of human phosphoglycerate mutase PMID: 15388943
- Phosphoglycerate mutase may have a role in preventing non-spherocytic anemia [case report] PMID: 15710582
- We report two patients in whom phosphoglycerate mutase (PGAM) deficiency was associated with the triad of exercise-induced cramps, recurrent myoglobinuria, and tubular aggregates in the muscle biopsy. PMID: 16881065
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蛋白家族：Phosphoglycerate mutase family, BPG-dependent PGAM subfamily
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組織特異性：Expressed in the liver and brain. Not found in the muscle.
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數據庫鏈接：
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