PCYT1A Recombinant Monoclonal Antibody
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中文名稱：PCYT1A重組抗體
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貨號：CSB-RA213218A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: PCYT1A antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa -
Immunofluorescence staining of Hela Cells with CSB-RA213218A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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其他：
產品詳情
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產品描述：CSB-RA213218A0HU是針對PCYT1A靶點研發的重組單克隆抗體，經多種實驗驗證具有優異性能。PCYT1A（磷酸膽堿胞苷酰轉移酶1A）是催化磷脂酰膽堿合成的限速酶，參與細胞膜形成、脂質代謝及信號轉導等基礎生物學過程。本抗體經ELISA驗證可特異性識別天然及重組PCYT1A蛋白，在Western Blot實驗中能清晰檢測內源性PCYT1A表達，推薦使用1:500-1:5000稀釋比例；免疫熒光實驗顯示其在細胞質中呈現特異性定位信號，推薦1:20-1:200稀釋范圍。該抗體采用重組表達技術制備，具有高親和力與批次間一致性，適用于探究PCYT1A在脂代謝調控、細胞膜動態平衡及相關疾病模型中的作用機制，可廣泛應用于生物化學、細胞生物學等領域的基礎研究，包括但不限于肝臟脂質穩態研究、神經細胞膜功能分析以及代謝通路相互作用探索等實驗場景。
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Uniprot No.：
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基因名：PCYT1A
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別名：Choline-phosphate cytidylyltransferase A (EC 2.7.7.15) (CCT-alpha) (CTP:phosphocholine cytidylyltransferase A) (CCT A) (CT A) (Phosphorylcholine transferase A), PCYT1A, CTPCT PCYT1
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反應種屬：Human
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免疫原：A synthesized peptide derived from human PCYT1A
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：4F2
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IF 1:20-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis.
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基因功能參考文獻：
- There is no correlation between single PCYT1A rs712012 and PCYT1A rs7639752 polymorphisms and the incidence of intrauterine fetal death. PMID: 28509322
- PCYT1A mutations were identified in patients with isolated retinal dystrophy without any skeletal involvement from two Italian families. PMID: 28272537
- CCT contributes to phospholipid compositional homeostasis. [Review] PMID: 26165797
- PCYT1A-generated phosphatidylcholine has a role in the normal function of white adipose tissue and insulin action PMID: 24889630
- We report loss-of-function mutations in PCYT1A as the cause of spondylometaphyseal dysplasia with cone-rod dystrophy. PMID: 24387990
- Mutations in PCYT1A cause spondylometaphyseal dysplasia with cone-rod dystrophy PMID: 24387991
- N-Methylaspartate induced nitric oxide synthase activation and nuclear factor-kB subunit p65 nuclear translocation in A549 cells were responsible for decreased CTP:phosphocholine cytidylyltransferase A expression PMID: 20661636
- Analyses showed genotype effects of PCYT1A genes on spina bifida risk, but did not show evidence of gene-nutrient. interactions. PMID: 17184542
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相關疾病：Spondylometaphyseal dysplasia with cone-rod dystrophy (SMDCRD)
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亞細胞定位：Cytoplasm, cytosol. Membrane; Peripheral membrane protein. Endoplasmic reticulum. Nucleus.
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蛋白家族：Cytidylyltransferase family
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組織特異性：Brain, placenta, liver, fetal and adult lung.
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數據庫鏈接：
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