MME Recombinant Monoclonal Antibody

說明書

中文名稱：

MME重組抗體
貨號：

CSB-RA187479A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA187479A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA187479A0HU MME重組單克隆抗體是針對膜金屬內肽酶（MME/CD10/NEP）研發的高特異性檢測工具，經嚴格驗證適用于ELISA和免疫組化（IHC）實驗，其中IHC推薦使用稀釋比為1:50-1:200。該抗體靶向的MME蛋白作為重要的細胞表面鋅金屬蛋白酶，參與調控神經肽、炎癥因子及生長因子的活性，在淋巴細胞分化、上皮屏障功能維持及腫瘤微環境調控中發揮關鍵作用。實驗數據顯示，該抗體在多種組織樣本中呈現清晰的膜定位染色效果，尤其在腎臟近端小管、腸道上皮及淋巴組織中表現出優異的目標蛋白識別能力，且在重復實驗中保持批次間高穩定性。本產品適用于基礎科研中MME蛋白表達模式分析、腫瘤微環境研究（如乳腺癌、腎癌等實體瘤相關研究）以及免疫細胞分化機制探索，為研究者提供可靠的實驗工具支持。所有實驗數據均通過陽性和陰性對照驗證，確保結果的準確性和可重復性。
Uniprot No.：

P08473
基因名：

MME
別名：

Neprilysin (EC 3.4.24.11) (Atriopeptidase) (Common acute lymphocytic leukemia antigen) (CALLA) (Enkephalinase) (Neutral endopeptidase 24.11) (NEP) (Neutral endopeptidase) (Skin fibroblast elastase) (SFE) (CD antigen CD10), MME, EPN
反應種屬：

Human
免疫原：

A synthesized peptide derived from human CD10
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

10G11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
基因功能參考文獻：
1. showed CD10 positivity in fibroblast-like stromal cells and fibrous material PMID: 29791034
2. these data indicated under cigarette smoke condensate treatment; losing of membrane p120ctn could upregulate surface NEP protein level and thus facilitate BEAS-2B cell migration. PMID: 30249887
3. High expression of CD10 is associated with lymph node invasion in colorectal cancer. PMID: 29653092
4. We observed an increase of VCAM-1 urine levels in diabetic nephropathy-basal patients compared to diabetic controls and an increase of urinary neprilysin in DN-treated patients with persistent albuminuria PMID: 29854824
5. Degradation of tropoelastin and skin elastin by neprilysin PMID: 29196110
6. Xanthorrhizol (Xan) reduced 4-hydroxynonenal (HNE) levels on NEP proteins and preserved enzymatic activities of neprilysin (NEP) in HNE- or oligomeric Abeta42-treated cells. Xan reduced Abeta42 accumulation and protected neurones against oligomeric Abeta42-induced neurotoxicity through preservation of NEP activities PMID: 29330223
7. These findings support the role of the stromal CD10 expression in breast cancer progression and dissemination, and suggest a relationship with cancer stem cells. PMID: 29306324
8. Aberrant CD10 and BCL6 expression defines a subset of MCLs with higher mean Ki-67 index and higher prevalence of MUM1 expression PMID: 28628241
9. Our study firstly confirmed the association of MME miRNA binding site polymorphism with the risk of LOAD. However, the association results warrant further validation. PMID: 28294061
10. the Ras signaling pathway is involved in HIV-1 Tat-induced changes in ZO-1 and NEP. PMID: 28553432
11. In this meta-analysis of the Han Chinese population, neprilysin confers genetic susceptibility to Alzheimer's disease in Han Chinese populations. PMID: 26362309
12. High CD10 expression is associated with lymphoma in Waldeyer ring. PMID: 27616053
13. Rare Variants in MME, Encoding Metalloprotease Neprilysin, Are Linked to Late-Onset Autosomal-Dominant Axonal Polyneuropathies PMID: 27588448
14. CD10 down expression in follicular lymphoma correlates with gastrointestinal lesion involving the stomach and large intestine PMID: 27513891
15. High CD10 expression is associated with Basal cell carcinoma of the skin. PMID: 27039776
16. the dynamic behavior of human NEP and NEP2 proteins was monitored by conducting molecular dynamics (MD) simulations. PMID: 26846903
17. This study reveals some unusual findings in otherwise classical disease entity, like absence of palpable spleen, presence of lymphadenopathy, normal or elevated leukocyte counts, expression of CD10, which at times could be diagnostically challenging. PMID: 26609034
18. Case Report: endometrial mixed carcinoma with the neuroendocrine component expressing CD10 that showed a long survival. PMID: 26830028
19. CD10 and Bcl2 expression in tumor cells could give convincing diagnostic value to distinguish squamous cell carcinoma from seborrheic keratosis PMID: 26573127
20. The peptide qf-Abeta(12-18)C (sequence VHHQKLVC) was cleaved by multiple Abeta-degrading enzymes, including NEP, ACE, and ECE-1, whereas the redesigned peptide qf-Abeta(12-16)AAC (sequence VHHQKAAC) was sensitive to NEP and ACE only. PMID: 27096746
21. Loss-of-function MME mutations are the most frequent cause of adult-onset autosomal-recessive Charcot-Marie-Tooth disease type 2 in Japan. PMID: 26991897
22. CD10 positivity is luminal/membranous in most benign apocrine breast lesions, the staining being nonuniversal and sometimes focal; analogous staining in apocrine malignancies seems rarer in DCIS and even rarer in invasive apocrine carcinomas, but atypical cytoplasmic positivity may also occur PMID: 26562027
23. These findings indicate that CD10 may promote tumor progression by regulating the expression profiles of genes related to cell proliferation, angiogenesis, and resistance to apoptosis. PMID: 26881775
24. CD10 gene expression plays a role in the pathogenesis of diffuse large B-cell lymphoma. PMID: 26414904
25. High tumoral CD10 expression correlates with aggressive histology in patients with malignant pleural mesothelioma. PMID: 25608772
26. In stage I lung adenocarcinoma, tumoral CD10 correlated with high-grade histology and was an independent predictor of recurrence in intermediate-grade tumors. PMID: 26141216
27. CD10 strongly labelled only the gastrointestinal cells, with a well-defined apical membrane signal PMID: 24754336
28. High CD10 expression is associated with Phylloides Tumors. PMID: 25921112
29. The expression of neprilysin is increased in glioma cells following 5-HT2C activation. PMID: 25452160
30. CD10 expression is related to a distinct gene expression signature in mantle cell lymphoma cases, but is without clinical or biological implications. PMID: 26124315
31. Correlation between CD10 stromal expression and disease-free survival rate in breast cancer patients. PMID: 23575921
32. E-cadherin and CD10 in endometrial lesions is not correlated, but reduced expression of both molecules could be critical for progression of endometrial carcinoma PMID: 25282623
33. Evaulate CD10 and mucin expression in relation to microsatellite instability/mismatch repair proteins in 47 cases of small bowel adenocarcinoma. PMID: 25759539
34. This meta-analysis indicates that rs3736187 (A/G) polymorphisms may be a potential beneficial single nucleotide polymorphism (SNP), which are associated with a decreased risk in Alzheimer disease. PMID: 25125048
35. Follicular lymphoma CD10(pos) follicular helper T cells specifically display an IL-4(hi)IFN-gamma(lo) cytokine profile and encompass the malignant B-cell-supportive follicular helper T cells subset. PMID: 25733581
36. Letter: suggest that CD10 is not a useful marker to differentiate seminoma from non-seminomatous germ cell tumours. PMID: 25713420
37. This study gives substantial proof to the various models/research papers explaining the role of CD10 in breast cancer pathogenesis. PMID: 25308002
38. Immunohistochemical distinction of renal cell carcinoma from other carcinomas with clear-cell histomorphology: utility of CD10 and CA-125 in addition to PAX-2, PAX-8, RCCma, and adipophilin. PMID: 25279712
39. Serum CD10 levels might serve as a useful marker of synchronous and metachronous liver metastasis in colorectal cancer. PMID: 24972738
40. PKCepsilon activation may have therapeutic efficacy for AD by reducing neurotoxic Abeta accumulation as well as having direct anti-apoptotic and synaptogenic effects PMID: 24848988
41. High CD10 expression is associated with squamous cell carcinoma. PMID: 24895167
42. MME expression level was differentially altered in Crohn disease and ulcerative colitis patients. PMID: 23827863
43. the recombinant brain-targeted neprilysin, ASN12, may be an effective treatment for AD and warrant further investigation in clinical trials. PMID: 24825898
44. Expression of CD10 is associated with therapeutic resistance and cancer stem cell-like properties of head and neck squamous cell carcinoma. PMID: 24874475
45. A direct up-regulation of stroma fibroblast MME expression under hypoxia might contribute to enhanced aggressiveness of hypoxic cancers. PMID: 24460801
46. Data indicate an important, previously neglected, role of NEP for regulation of luminal factors in the epididymis and suggest a novel role for CNP/guanylyl cyclase B in the epididymal epithelium. PMID: 24099862
47. only seminomas and intratubular germ cell neoplasia, the precursors of germ cell tumours, express CD10 PMID: 23857215
48. study suggests that the severity of periodontal disease may be associated with the expression of metalloendopeptidase genes, including NEP, ECE1 and ADAM17, in the buccal mucosal epithelium. PMID: 23360525
49. CD10 might be involved in the development of endometriosis due to its influence on CD44-dependent cell adhesion. PMID: 23653392
50. Overexpression of membrane metalloendopeptidase inhibits substance P stimulation of cholangiocarcinoma growth. PMID: 24603459
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相關疾病：

Charcot-Marie-Tooth disease 2T (CMT2T); Spinocerebellar ataxia 43 (SCA43)
亞細胞定位：

Cell membrane; Single-pass type II membrane protein.
蛋白家族：

Peptidase M13 family
數據庫鏈接：

HGNC: 7154

OMIM: 120520

KEGG: hsa:4311

STRING: 9606.ENSP00000353679

UniGene: Hs.307734

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