MAOB Recombinant Monoclonal Antibody

中文名稱：

MAOB重組抗體
貨號：

CSB-RA999377A0HU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in: L02 whole cell lysate, HepG2 whole cell lysate
All lanes: Monoamine Oxidase B Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 59, 47 kDa
Observed band size: 59 kDa

IHC image of CSB-RA999377A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA999377A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產(chǎn)品詳情
產(chǎn)品評價
引用文獻(xiàn)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA999377A0HU MAOB重組單克隆抗體是針對單胺氧化酶B（MAOB）研發(fā)的高特異性檢測工具，適用于多種實(shí)驗(yàn)場景。該抗體通過重組技術(shù)制備，具有優(yōu)異的批間一致性，經(jīng)嚴(yán)格驗(yàn)證可在ELISA、蛋白質(zhì)印跡（WB）及免疫組化（IHC）等多種平臺穩(wěn)定應(yīng)用。在蛋白質(zhì)印跡實(shí)驗(yàn)中，抗體在1:500至1:5000稀釋范圍內(nèi)可清晰識別內(nèi)源性MAOB蛋白，呈現(xiàn)單一特異性條帶；在免疫組化分析中，1:50至1:200稀釋比例下能精準(zhǔn)定位石蠟包埋組織切片中的靶蛋白表達(dá)。MAOB作為線粒體酶參與神經(jīng)遞質(zhì)代謝調(diào)控，與神經(jīng)退行性疾病及情緒調(diào)節(jié)密切相關(guān)。本產(chǎn)品可為神經(jīng)生物學(xué)研究提供可靠支持，尤其適用于帕金森病機(jī)制探索、抗抑郁藥物體外評價及腦組織/細(xì)胞模型中MAOB表達(dá)水平檢測等科研方向，為相關(guān)領(lǐng)域的蛋白功能研究和病理機(jī)制解析提供優(yōu)質(zhì)實(shí)驗(yàn)材料。
Uniprot No.：

P27338
基因名：

MAOB
別名：

Amine oxidase [flavin-containing] B (EC 1.4.3.4) (Monoamine oxidase type B) (MAO-B), MAOB
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from human Monoamine Oxidase B
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

9B6
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文獻(xiàn)

Effects of latroeggtoxin-VI on dopamine and α-synuclein in PC12 cells and the implications for Parkinson's disease D Yu,Biological research,2024

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.
基因功能參考文獻(xiàn)：
1. Our observations provide new information on the genotype-phenotype relations of MAOA/B and EFHC2 genes involved in the contiguous deletions of Norrie disease.Based on the case of our observation, contiguous deletion with only one of the MAO genes (MAOB) may not cause psychomotor disability, and deletion of EFHC2may not contribute to epilepsy. PMID: 29321361
2. This study shows that MAO-B levels are increased not only in astrocytes but also in pyramidal neurons in Alzheimer disease brain and also suggests that MAO-B regulates Abeta production in neurons via gamma-secretase PMID: 28764767
3. The findings may suggest that MAO-B is an important sensor in iron-stressed neuronal cells. PMID: 28685208
4. brain protein levels of MAOB are normal or elevated in the three parkinsonian conditions-with MAOB increase generally associated with elevations in levels of astrocyte markers. Brain MAOA concentrations were, somewhat surprisingly, not decreased in Parkinson's disease, Progressive Supranuclear palsy or multiple system atrophy with the exception of the atrophic putamen in MSA, despite loss of dopamine neurons. PMID: 29050386
5. MAOB gene variants are contributing to the etiology of ADHD in the Indo-Caucasoid population PMID: 27341797
6. This study suggests that MAOB increases ASD risk in males, possibly through its sex-specific regulatory effect on 5-HT metabolism and behavior. PMID: 27381555
7. These findings suggest an association of platelet MAO-B activity, but a lack of association of MAOB rs1799836 and MAOA-uVNTR, with selected psychotic symptoms in ethnically homogenous veterans with PTSD. PMID: 27112218
8. No significant association was found between severe agitation and MAOA uVNTR or MAOB rs1799836 polymorphism, revealing that these individual polymorphisms in MAO genes are not related to severe agitation in subjects with schizophrenia and conduct disorder. Higher platelet MAO-B activity was found in subjects with severe agitation vs. non-agitated subjects. Platelet MAO-B activity was not associated with MAOB rs1799836 PMID: 26851573
9. we performed a meta-analysis in the Chinese population to provide comprehensive data on the association between the MAOB polymorphism and Parkinson's disease. Overall, no significant association was found between the MAOB A644G polymorphism and Parkinson's disease risk in the Chinese population. PMID: 27421021
10. inhibition of MAO-B using selegiline reversed the cigarette smoke-induced oxidative stress and inflammation in bronchial epithelial cells. PMID: 28108387
11. Monoamine oxidase B levels are highly expressed in gliomas. PMID: 26689994
12. The results of this meta-analysis suggest that people in the Asian population with the MAOB intron 13 A allele have an increased risk of Parkinson disease, especially when combined with the COMT LL genotype. PMID: 26000819
13. Report isolation of MOA-B inhibitors from Vernonia cinerea. PMID: 25857233
14. MAOB A644G polymorphism was associated with Parkinson Disease risk and a subgroup analyses by ethnicity and gender also found significant relationships between this polymorphism and PD risk. PMID: 24658549
15. Data (including data from molecular dynamics simulation studies) suggest that the protonation state of the active site residue Lys296 in monoamine oxidase B does not have influence on the hydride transfer reaction in the metabolism of dopamine. PMID: 25220264
16. Polymorphisms of COMT and MAO-B genes has a unique characteristics among Chinese patients with Parkinson's disease. They may be related with differences in drug response in such patients. PMID: 25636089
17. There is significantly decreased expression of MAO-A and MAO-B in cancerous tissues when compared with adjacent noncancerous tissues. PMID: 25389533
18. The results of this study suggested that polymorphisms in MAOB may increase the risk of wearing-off and dyskinesias. PMID: 25034874
19. study revealed increased platelet MAO-B activity in patients with alcohol dependence; suggests increased platelet MAO-B activity might be used as independent peripheral indicator of alcohol dependence PMID: 25035107
20. We found significant negative correlations regarding the expression of the genes COMT, MAOB, DRD4, DRD5 and FOS, indicating that increased schizotypy coincides with higher levels of dopaminergic dysregulation on the mRNA-level. PMID: 24630741
21. Our results confirmed that SNPs in DRD4 and COMT, but not DBH or MAOB are associated with autism and ADHD in small, ethnically homogeneous groups of non-related male Caucasians. PMID: 24210742
22. genetic variants of COMT, MAOB and DRD2 loci modulate susceptibility to PD in South Indian subjects PMID: 24772965
23. Data indicate that in vitro MAO-B inhibitory activity by synthesized (coumarin-3-yl)carbamates is comparable with that of the selegiline. PMID: 23474901
24. The present data suggest that pharmacokinetic or pharmacodynamic factors other than the investigated genetic variants of the MAOB and COMT enzymes seem to determine the response to levodopa in the Iranian PD patients. PMID: 23093014
25. data may suggest that the MAOB and COMT genetic polymorphisms do not play any role in the pathogenesis of Parkinson's disease in Iranians PMID: 23319194
26. The rs1799836 SNP did not appear to correlated with attentional bias for facial expressions. PMID: 23054588
27. results provide additional evidence that GABRB3 and MAOB/NDP gene regions might constitute risk factors for hallucinations and delusions in schizophrenia. PMID: 22414661
28. G/A dimorphism in intron 13 sequence creates splicing enhancer thus stimulating intron 13 removal efficiency PMID: 22974659
29. [review] While MAO-B primarily serves in the catabolism of 2-phenylethylamine and contributes to the degradation of other trace amines and dopamine, MAO-A has high affinity for serotonin and norepinephrine. PMID: 21971001
30. Mao-B platelet protein level may serve as a biomarker for age-related dementia, especially AD. PMID: 22270014
31. [review] A better understanding of the transcriptional regulation of MAO B may help explain the differential tissue-specific expression of the two isoenzymes and provide insights into the molecular basis of the disorders associated with MAO dysfunction. PMID: 21359973
32. Results suggest MAOB is a susceptibility gene for schizophrenia in Han Chinese. PMID: 21978760
33. results demonstrate that the bipartite cavity structure in MAO B plays an important role in substrate and inhibitor recognition to distinguish its specificities from those of MAO A PMID: 21978362
34. Nitrogen kinetic isotope effects for the oxidation of benzylamine and (1,1-(2)H(2))benzylamine by recombinant human monoamine oxidase B show that cleavage of the CH bond is not concerted with rehybridization of the nitrogen atom. PMID: 21786798
35. mutation in gene encoding the monoamine degradation enzyme monoamine oxidase B was found in SCZ. PMID: 20479760
36. COMT rs4680 and MAOB rs1799836 polymorphisms may increase susceptibility to Parkinson's disease risk among Japanese PMID: 21781348
37. In early stage Alzheimer disease, discrete MAO-B reduction reflects the capacity of the population of MAO-B-positive progenitor cells to adapt to the neurodegenerative process. PMID: 20648649
38. a monoamine-oxidase epigenetic regulation is involved in effects of smoking PMID: 19956754
39. Children with different subtypes of ADHD had significantly lower platelet MAO-B activity than control children. PMID: 20022119
40. Data show that lower p-MAO-B associated with increased inattention scores (Conners' teacher-rating scale) and lower l-MR associated with increased score for oppositional-defiant disorder. PMID: 19863190
41. first evidence that MAOB polymorphisms influence levels of negative emotionality in healthy human volunteers, may lead to a better understanding of personality traits and susceptibility to developing psychiatric disorders such as major depression PMID: 19657584
42. Inflammation of the dental pulp was accompanied by a significant decrease in MAO labeling, MAO B (but not MAO A) activity and the increase in SSAO activity. PMID: 19789505
43. X ray structure to 3 A resolution PMID: 11753429
44. Association of monoamine oxidase A gene polymorphism with Alzheimer's disease and Lewy body variant. PMID: 12098640
45. a strong gender difference exists with respect to the modifying effect of MAO-B genotype on the smoking association with parkinson disease PMID: 12428723
46. MAO-B polymorphisms are associated with smoking behaviour PMID: 12563176
47. There was no interaction of smoking and the G allele and risk of Parkinson disease. PMID: 12815741
48. molecular models and binding sites of inhibitors PMID: 12825788
49. MAO B gene expression is selectively induced by a decreased Sp3/Sp1 ratio and reduced DNA methylation PMID: 12855685
50. The 1.7-A structure of the reversible isatin-MAO-B complex has been determined; it forms a basis for the interpretation of the enzyme's structure when bound to either reversible or irreversible inhibitors. PMID: 12913124
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亞細(xì)胞定位：

Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.
蛋白家族：

Flavin monoamine oxidase family
數(shù)據(jù)庫鏈接：

HGNC: 6834

OMIM: 309860

KEGG: hsa:4129

STRING: 9606.ENSP00000367309

UniGene: Hs.654473

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