ILF3 Recombinant Monoclonal Antibody

說明書

中文名稱：

ILF3重組抗體
貨號：

CSB-RA289890A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA289890A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA289890A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of Hela Cells with CSB-RA289890A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
其他：

產品詳情
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靶點詳情

產品詳情

產品描述：

ILF3 Recombinant Monoclonal Antibody（CSB-RA289890A0HU）是一款針對人源ILF3蛋白研制的高特異性抗體。ILF3（Interleukin enhancer-binding factor 3）是一種多功能核酸結合蛋白，參與RNA穩定性調控、先天免疫應答及細胞周期進程，在病毒感染應答和腫瘤微環境調控中發揮重要作用。該抗體通過重組表達技術制備，經ELISA、免疫組化（IHC）和免疫熒光（IF）嚴格驗證，在IHC實驗中推薦1:50-1:200稀釋度可清晰顯示組織樣本中ILF3的亞細胞定位，IF實驗在1:20-1:200范圍內呈現高信噪比信號，適用于細胞核與細胞質內靶蛋白的可視化分析。其優異的批次一致性為RNA代謝研究、宿主-病原體互作機制探索以及癌癥相關信號通路分析提供可靠工具，尤其適用于探究ILF3在DNA損傷修復、病毒復制抑制或腫瘤免疫逃逸中的分子機制。本產品適用于體外實驗模型中的蛋白表達檢測，為基因調控網絡研究和疾病相關分子標記物發現提供技術支持。
Uniprot No.：

Q12906
基因名：

ILF3
別名：

Interleukin enhancer-binding factor 3 (Double-stranded RNA-binding protein 76) (DRBP76) (M-phase phosphoprotein 4) (MPP4) (Nuclear factor associated with dsRNA) (NFAR) (Nuclear factor of activated T-cells 90 kDa) (NF-AT-90) (Translational control protein 80) (TCP80), ILF3, DRBF MPHOSPH4 NF90
反應種屬：

Human
免疫原：

A synthesized peptide derived from human ILF3
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

7H8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs. As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response. Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs.
基因功能參考文獻：
1. DNA- and RNA-binding protein NF90/ILF3/NF110 associates with chromatin to hierarchically regulate transcription factors that promote proliferation and suppress differentiation. PMID: 29590119
2. Reprot a coordinated regulation of circRNA biogenesis and function by NF90/NF110 in viral infection. PMID: 28625552
3. The NF90 regulates PARP1 mRNA stability in hepatocellular carcinoma cells, and NF90 is a potential target to inhibit PARP1 activity. PMID: 28487110
4. Variants in ILF3 gene is associated with thrombosis. PMID: 26908601
5. these data establish miR-590-5p as an anti-onco-miR that inhibits colorectal cancer angiogenesis and metastasis through a new mechanism involving NF90/VEGFA signaling axis. PMID: 27735951
6. NF90 exerts its antiviral activity by antagonizing the inhibitory role of NS1 on PKR phosphorylation PMID: 27423063
7. These data suggest that, like ADAR2, underlying sequences in dsRNA may influence how NF90 recognizes its target RNAs PMID: 26712564
8. The NF90 C-terminus is involved in conformational changes in the protein after RNA binding. PMID: 26795062
9. Taken together, our discovery of the function of TCP80 and RHA in regulating p53 IRES and p53 induction following DNA damage provides a better understanding of the mechanisms that regulate IRES-mediated p53 translation in response to genotoxic stress. PMID: 26273641
10. data suggest that lncRNA SALNR modulates cellular senescence at least partly through changing NF90 activity PMID: 26491010
11. Data indicate the role of cyclin-dependent kinase inhibitor 1A (p21) and interleukin enhancer binding factor 3 (ILF3) pathway in mediating the anti-leukemic activity of sodium dichloroacetate (DCA) in oncoprotein p53null leukemic cells. PMID: 25544776
12. Suppression of NF90 caused a decrease in the half-life of cyclin E1 mRNA PMID: 25399696
13. the role of the human NF90 protein isoforms in DNA and RNA virus replication PMID: 25447144
14. NF90 exerts antiviral activity through regulation of PKR phosphorylation and stress granules in infected cells. PMID: 24623135
15. The function of NF90 in posttranscriptional gene regulation and microRNA biogenesis. [Review] PMID: 23965975
16. Data indicate that knockdown of interleukin enhancer-binding factor 3 (ILF3) leads to the increased levels of mature urokinase-type plasminogen activator (uPA) mRNA-targeting miRNAs miR-193a, miR-193b and miR-181a. PMID: 22986534
17. contributes to maintaining low levels of senescence-associated secretory phenotype factors in non-senescent cells PMID: 23117626
18. The unique C-terminal region of ILF3/NF110 is important for promoting survivin expression and for high affinity binding to YM155. PMID: 22442257
19. high expression of ERCC1, XPB and ILF3 was observed in human epithelial ovarian cancer PMID: 21971700
20. These data suggest that DRBP76, via its association with VP35, exerts an anti-Ebola virus function. PMID: 21987769
21. The protein polymorphism of Ilf3/NF90 and the various subcellular localizations of their isoforms may partially explain the various functions previously reported for these proteins. PMID: 21811582
22. associations of rs2569512 of ILF3 to myocardial infarction among individuals stratified by the absence or presence of hypertension, diabetes mellitus and chronic kidney disease PMID: 21347509
23. Production of HIV particles is regulated by altering sub-cellular localization and dynamics of Rev induced by double-strand RNA binding protein. PMID: 21364984
24. NF90 binds the dengue virus RNA 3' terminus and is a positive regulator of dengue virus. PMID: 21386893
25. The relationship between rs6929846 of BTN2A1 or rs2569512 of ILF3 and myocardial infarction is influenced by the serum concentrations of high density lipoprotein cholesterol and low density lipoprotein cholesterol, respectively. PMID: 21468600
26. Data suggest that physiologic DRBP76 expression, isoform distribution and subcellular localization are profoundly altered upon malignant transformation. PMID: 20668518
27. Nuclear export of this protein is required for interleukin-2 mRNA stabilization. PMID: 12504009
28. Results describe the expression of four isoforms of nuclear factor 90, including NF110, by cDNA cloning and mass spectrometric analysis of proteins isolated from human cells PMID: 12946349
29. nuclear export of ILF3 is mediated by exportin-5 and minihelix-containing RNA PMID: 14570900
30. the multiple cellular functions, i.e., translation control, interleukin-2 enhancer binding, or cell division, of ILF3 are fulfilled by alternatively spliced isoforms. PMID: 14654356
31. NF90 and NF110 engage RNA differentially and translocate from the nucleus to the cytoplasm in mitosis PMID: 15811368
32. ADAR1 has the potential both to change information content through editing of mRNA and to regulate gene expression through interacting with NF90 PMID: 16055709
33. The human Nuclear Factor 90 c-terminal variant protein encoded by ILF3 interacts with Rev to inhibit Rev-mediated RNA transport. PMID: 17125513
34. Our results show that perturbation Tat/TAR RNA interaction by the dsRNA binding nuclear factor 90 protein is sufficient to inhibit transcriptional activation of HIV-1. PMID: 17565699
35. DRBP76/NF90 isoform facilitates VEGF expression by promoting VEGF mRNA loading onto polysomes PMID: 18039850
36. NFARs exert influence on mRNA trafficking and the modulation of translation rates and may constitute an innate immune translational surveillance mechanism important in host defense countermeasures against virus infection PMID: 18337511
37. MKP-1 upregulation by oxidative stress is potently influenced by increased mRNA stability and translation, mediated at least in part by the RNA-binding proteins HuR and NF90. PMID: 18490444
38. ILF-3, which has been known to regulate IL-2 expression in T cells, up-regulates synoviolin expression with GABPalpha in rheumatoid synovial cells PMID: 19116932
39. NF90 inhibits influenza virus replication during the early phase of infection through direct interaction with viral NP. PMID: 19494010
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亞細胞定位：

Nucleus, nucleolus. Cytoplasm. Nucleus.
組織特異性：

Ubiquitous.
數據庫鏈接：

HGNC: 6038

OMIM: 603182

KEGG: hsa:3609

STRING: 9606.ENSP00000404121

UniGene: Hs.465885

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