IKBKB Recombinant Monoclonal Antibody

說明書

中文名稱：

IKBKB重組抗體
貨號：

CSB-RA256500A0HU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in: K562 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate
All lanes: IKK beta antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87 kDa
Observed band size: 87 kDa

IHC image of CSB-RA256500A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of Hela Cells with CSB-RA256500A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA256500A0HU IKBKB重組單克隆抗體是針對NF-κB信號通路關(guān)鍵激酶IKKβ（Inhibitor of nuclear factor kappa-B kinase subunit beta）開發(fā)的高特異性科研試劑。該抗體經(jīng)ELISA、WB、IHC、IF等多種實驗平臺嚴(yán)格驗證，在蛋白免疫印跡中推薦使用1:500-1:5000稀釋比例，免疫組化建議1:50-1:200，免疫熒光實驗適用1:20-1:200濃度范圍，實驗數(shù)據(jù)顯示其能精準(zhǔn)識別內(nèi)源性IKBKB蛋白，具有出色的批間一致性和低交叉反應(yīng)性。IKBKB作為調(diào)控NF-κB活化的核心激酶，在炎癥反應(yīng)、細(xì)胞凋亡和腫瘤發(fā)生等生物學(xué)過程中發(fā)揮重要作用，本產(chǎn)品可廣泛應(yīng)用于該信號通路的機制研究、疾病相關(guān)蛋白表達(dá)水平檢測以及亞細(xì)胞定位分析等基礎(chǔ)科研領(lǐng)域，特別適用于癌癥生物學(xué)、免疫調(diào)控及相關(guān)病理模型的實驗體系探索。
Uniprot No.：

O14920
基因名：

IKBKB
別名：

Inhibitor of nuclear factor kappa-B kinase subunit beta (I-kappa-B-kinase beta) (IKK-B) (IKK-beta) (IkBKB) (EC 2.7.11.10) (I-kappa-B kinase 2) (IKK2) (Nuclear factor NF-kappa-B inhibitor kinase beta) (NFKBIKB), IKBKB, IKKB
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from human IKK beta
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

3E1
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death. Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus.
基因功能參考文獻(xiàn)：
1. The results demonstrate that the inhibition of miR-16 renders resistance to paclitaxel in vitro and in vivo by targeting IKBKB via NF-kappa B signaling pathway. PMID: 29935185
2. Expression of TAp63, IKKbeta and XBP1s is also increased in livers of obese patients with liver steatosis. PMID: 28480888
3. IKK2 and NF-kappaB are related to poor prognosis and are potential predictors of response to platinum-based chemotherapy in high grade serous carcinoma PMID: 29254797
4. These studies identify functional EREs in the IKKB promoter and identify IKKB as an ERalpha and NSC35446.HCl-regulated gene, and they suggest that NF-kappaB and IKKB, which were previously linked to antiestrogen resistance, are targets for NSC35446.HCl in reversing antiestrogen resistance. PMID: 28808806
5. Curcumin suppressed CXCL5 expression by direct inhibition of IKKbeta phosphorylation, and inhibition of p38 MAPK via induction of negative regulator MKP-1. PMID: 27538525
6. This study demonstrated that nerve injury-induced Csf1 upregulation was ameliorated indicating that IKK/NF-kappaBeta-dependent SGC activation induced Csf1 expression in sensory neurons. PMID: 28722693
7. Findings uncover a novel function for IKKbeta/mHTTx1 interactions in regulating IL-34 production, and implicate a role for IL-34 in non-cell-autonomous, microglial-dependent neurodegeneration in HD. PMID: 28973132
8. HOTAIR operates the action of IKKalpha, IKKbeta, IKKgamma in liver cancer stem cells PMID: 27367027
9. These results suggested that APN ameliorated endothelial inflammation and IR through ROS/IKKbeta pathway. PMID: 27639126
10. p300-dependent histone H3 acetylation and C/EBPbeta-regulated IKKbeta expression contribute to thrombin-induced IL-8/CXCL8 expression in human lung epithelial cells. PMID: 28428115
11. EGFR/PI3K/Akt/mTOR/IKK-beta/NF-kappaB signaling promotes head and neck cancer progression. PMID: 26895469
12. our results illustrate a new direction in nitrosourea treatment, and reveal that the combination of ROS-inducing IKKbeta inhibitors with nitrosoureas can be potentially exploited for melanoma therapy PMID: 28107677
13. Smad7 expression in necrotizing enterocolitis macrophages interrupts TGF-beta signaling and promotes NF-kappaB-mediated inflammatory signaling in these cells through increased expression of IKK-beta PMID: 26859364
14. High IKBKB expression is associated with prostate cancer. PMID: 27577074
15. Akt2, Erk2, and IKK1/2 phosphorylate Bcl3, converting Bcl3 into a transcriptional coregulator by facilitating its recruitment to DNA. PMID: 28689659
16. Rare variants in IKBKB are associated with decreased waist-to-hip ratio in European-Americans. PMID: 26757982
17. pVHL mediates K63-linked ubiquitination of IKKbeta, which plays a role in the regulation of IKK/NF-kappaB signalling. PMID: 27693634
18. our results demonstrate that miR-200b, a transcriptional target of NF-kappaB, suppresses breast cancer cell growth and migration, and NF-kappaB activation, through downregulation of IKBKB, indicating that miR-200b has potential as a therapeutic target in breast cancer patients. PMID: 26433127
19. The present research concluded that aspirin suppressed prostate cancer cell invasion by reducing MMP-9 activity and uPA expression through decreasing of IKK-beta-mediated NF-kappaB activation, indicating that the ability of aspirin to inhibit cell invasion might be useful in the chemoprevention of metastatic prostate cancer. PMID: 28278500
20. miR-429 is involved in regulation of the NF-kappaB pathway by targeting IKKbeta and functions as a tumor suppressor in cervical carcinogenesis. PMID: 27883176
21. TLR signaling led to lower expression of LRRC14. PMID: 27426725
22. results uncover a previously unidentified role of IKKbeta in regulating glycolysis, sensing low-glutamine-induced metabolic stress, and promoting cellular adaptation to nutrient availability. PMID: 27585591
23. data clearly demonstrate that KLHL21 negatively regulates TNFalpha-activated NF-kappaB signaling via targeting IKKbeta, providing new insight into the mechanisms underlying NF-kappaB regulation in cells. PMID: 27387502
24. Cis- and trans-gnetin H suppress cytokine response in LPS-stimulated THP-1 cells by preventing activation of key signaling molecules, IKK-beta, IkappaB alpha, and p65, involved in the NF-kappaB pathway. PMID: 27196294
25. The neuroprotective effects of celastrol and its analogues may be related to IKKbeta inhibition. PMID: 27931154
26. Survivin overexpression activates NFkappaB p65, which is important in the acquisition and maintenance of the oncogenic characteristics of esophageal squamous cell carcinoma. PMID: 26718331
27. Over-expressed IKK-Beta inhibits cell apoptosis in laryngeal squamous cell carcinoma. PMID: 26914121
28. DAT stabilized IkBa by inhibiting the phosphorylation of Ika by the IkB kinase (IKK) complex. DAT induced proteasomal degradation of TRAF6, and DAT suppressed IKKb-phosphorylation through downregulation of TRAF6 PMID: 26647777
29. IFIT5 promotes SeV-induced IKK phosphorylation and NF-kappaB activation by regulating the recruitment of IKK to TAK1. PMID: 26334375
30. Down-regulation of IKBKB expression and NFkappaB signaling in microglia/macrophages infiltrating glioblastoma correlates with defective expression of immune/inflammatory genes and M2 polarization that may result in the global impairment of anti-tumor immune responses in glioblastoma. PMID: 26427514
31. Combining bortezomib with IKK inhibitor is effective in treating ovarian cancer. PMID: 26267322
32. MyD88s is positively regulated by IKKbeta and CREB and negatively regulated by ERK1/2 signaling pathways. PMID: 26669856
33. IKK-beta suppresses GLI1 ubiquitination. PMID: 26603838
34. we demonstrate that one miR-497, is a likely negative regulator of IKKbeta PMID: 26020802
35. In cells with functional KEAP1, RTA 405 increased NRF2 levels, but not IKKb or BCL2 levels, and did not increase cell proliferation or survival. PMID: 26301506
36. findings indicate that the IKBKB and POLB SNPs confer no genetic predisposition to SLE risk in this Chinese Han population PMID: 26167925
37. Demonstrate the essential role of EGFR/Akt/IkappaBbeta/NF-kappaB pathway in the inhibitory effect of PA-MSHA on invasion and metastasis of HCC through suppressing EMT. PMID: 25066210
38. identified IKK-beta as a kinase capable of phosphorylating threonine 3 in N-terminal hungtingtin fragments PMID: 26106822
39. The activation of NF-kappaB induced translocation of AMAP1 to cytoplasm from cell membrane and nucleus, which resulted in augmented interaction of AMAP1 and IKKbeta PMID: 24865276
40. High IKBKB expression is associated with inflammation in heart Valve Diseases. PMID: 25630970
41. These studies not only reinforce the significance of maintaining a homeostatic balance of eNOS and IKKbeta within the cell system that regulates NO production, but they also confirm that the IKKbeta-Hsp90 interaction is favored in a high-glucose environment, leading to impairment of the eNOS-Hsp90 interaction, which contributes to endothelial dysfunction and vascular complications in diabetes. PMID: 25652452
42. IKKbeta regulates endothelial thrombomodulin in a Klf2-dependent manner PMID: 25039491
43. Studies indicate that transcription factor NF-kappaB plays a key role in numerous physiological processes, and its activation is tightly controlled by a kinase complex, IkappaB kinase (IKK). PMID: 25432706
44. Suppression of PKK expression by RNA interference inhibits phosphorylation of IKKalpha and IKKbeta as well as activation of NF-kappaB in human cancer cell lines; thus, PKK regulates NF-kappaB activation by modulating activation of IKKalpha and IKKbeta. PMID: 25096806
45. IKBKB-rs3747811AT single nucleotide polymorphism was associated with a significantly increased risk of developing wheezing. PMID: 25326706
46. IKKbeta is an IRF5 kinase that instigates inflammation PMID: 25326420
47. IKKbeta activates two "master" transcription factors of the innate immune system, IRF5 and NF-kappaB PMID: 25326418
48. RTK-mediated Tyr phosphorylation of IKKbeta has the potential to directly regulate NFkappaB transcriptional activation. PMID: 24386391
49. Expression of IKBKB gene reduced the cisplain sensitivity of A549 cells. PMID: 24854552
50. Gain-of function or knockdown of miR-200c in leiomyoma smooth muscle cells (LSMC) regulated IL8 mRNA and protein expression through direct targeting of IKBKB and alteration of NF-kB activity. PMID: 24755559
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相關(guān)疾病：

Immunodeficiency 15 (IMD15)
亞細(xì)胞定位：

Cytoplasm. Nucleus. Membrane raft.
蛋白家族：

Protein kinase superfamily, Ser/Thr protein kinase family, I-kappa-B kinase subfamily
組織特異性：

Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
數(shù)據(jù)庫鏈接：

HGNC: 5960

OMIM: 603258

KEGG: hsa:3551

STRING: 9606.ENSP00000430684

UniGene: Hs.597664

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