IDO1 Recombinant Monoclonal Antibody

說明書

中文名稱：

IDO1重組抗體
貨號：

CSB-RA215054A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA215054A0HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

IDO1重組單克隆抗體（產品編號：CSB-RA215054A0HU）是針對吲哚胺2,3-雙加氧酶1（IDO1）開發的高特異性科研用抗體。IDO1是色氨酸代謝通路中的關鍵限速酶，通過催化色氨酸分解為犬尿氨酸，參與調節免疫耐受微環境，在腫瘤免疫逃逸、自身免疫性疾病及慢性感染等病理過程中發揮重要作用。本抗體經ELISA和免疫組化（IHC）雙重驗證，在IHC應用中推薦使用1:50-1:200稀釋比例，能夠精準識別石蠟包埋組織切片中IDO1蛋白的表達定位，染色結果背景清晰、特異性顯著。其重組單克隆特性保證了批次間的高度一致性，適用于研究IDO1在腫瘤微環境中的分布特征、評估免疫檢查點調控機制，以及探索靶向IDO1的免疫聯合治療策略等相關基礎研究。該產品為深入解析IDO1介導的免疫抑制機制及其實驗室級檢測需求提供了可靠工具。
Uniprot No.：

P14902
基因名：

IDO1
別名：

Indoleamine 2,3-dioxygenase 1 (IDO-1) (EC 1.13.11.52) (Indoleamine-pyrrole 2,3-dioxygenase), IDO1, IDO INDO
反應種屬：

Human
免疫原：

A synthesized peptide derived from human INDO
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

9E5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity. Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection.
基因功能參考文獻：
1. Interference SNHG1 could inhibit the differentiation of Treg cells by promoting miR-448 expression and reducing IDO level, thereby impeding the immune escape of breast cancer . PMID: 29886172
2. We discuss how small-molecule inhibitors of the tryptophan (Trp) catabolic enzyme indoleamine 2,3-dioxygenase (IDO) represent a vanguard of new immunometabolic adjuvants to safely enhance the efficacy of cancer immunotherapy, radiotherapy, or 'immunogenic' chemotherapy by leveraging responses to tumor neoantigens PMID: 29413421
3. The role of IDO1-IDO2-AHR pathway in the TLR4-induced tolerogenic phenotype in human dendritic cells has been reported. PMID: 28256612
4. High coexpression of cytoplasmic IDO1/COX2 was found to be an independent predictor of poor outcome in colorectal cancer patients. PMID: 29853736
5. Report the crystal structures of IDO1 in complex with its substrate, Trp, an inhibitor, epacadostat, and/or an effector, indole ethanol (IDE). The data reveal structural features of the active site (Sa) critical for substrate activation; in addition, they disclose a new inhibitor-binding mode and a distinct small molecule binding site (Si). PMID: 29167421
6. IDO decreased glycolysis and glutaminolysis by activating GCN2K, resulting in activation of AMPactivated protein kinase. PMID: 29693118
7. this review highlights the role of indoleamine-2,3-dioxygenase in normal and pathological pregnancies PMID: 29154462
8. Data suggest that IDO1 is constitutively expressed in insulin-secreting cells from donors without diabetes; IDO1 appears to be down-regulated in insulin-containing beta-cells from double autoantibody-positive donors and donors with recent-onset type 1 diabetes; this study was conducted on donor tissues obtained from cadavers. PMID: 29945890
9. Twenty-nine percent (n = 2/7) of the PD-L1 positive poorly differentiated thyroid carcinomas also co-expressed IDO1 PMID: 29372535
10. In non-ST segment elevation myocardial infarction, the tolerogenic mechanism of the immune response related to IDO production by activated monocytes derived dendritic cells is altered, supporting their role in T-cell dysregulation. PMID: 29278387
11. These data suggest that the expression of immunosuppressive molecules, including PD-1 ligands and IDO1, by macrophage/microglia may be involved in immune evasion of lymphoma cells. PMID: 29998979
12. Our data demonstrate that high intratumoral IDO1 mRNA levels correlate with a poor glioblastoma (GBM) patient prognosis. It also confirms the positive correlation between increased GBM IDO1 levels and human-infiltrating T cells PMID: 28751450
13. Among the 89 patients, CD274, LAG3, and IDO1 expressions in TIICs were observed in 68.6% (61 cases), 13.5% (12), and 28.1% (25) of patients, respectively. CD274, CTLA4, and IDO1 were expressed in tumor cells of 24.7% (22 cases), 4.5% (4), and 72.0% (64) of patients, respectively. PMID: 29520442
14. Multivariate analysis indicated indoleamine 2,3-dioxygenase (IDO) expression as independent prognostic factors in overall survival (OS). PMID: 29848687
15. these findings suggest that IDO1 promoter methylation regulates anti-immune responses in breast cancer subtypes and could be used as a predictive biomarker for IDO1 inhibitor-based immunotherapy. PMID: 28264810
16. main biological and actionable difference between OSCC from NSND and SD lies in the immune microenvironment, suggesting a higher clinical benefit of PD-L1 and IDO1 inhibition in OSCC from NSND PMID: 28460011
17. An IDO1 inhibitor, epacadostat also demonstrated promising activity in combination with the PD-1 checkpoint inhibitors in other solid tumors, including melanoma, urothelial carcinoma, renal cell carcinoma, and non-small cell lung cancer PMID: 28760910
18. This first-in-human phase I study investigated the maximum tolerated dose, safety, pharmacokinetics, pharmacodynamics, and antitumor activity of epacadostat (INCB024360), a potent and selective inhibitor of IDO1 PMID: 28053021
19. Fumaric acid esters inhibit both IDO expression and enzymatic activity leading to a modulation of tryptophan degradation. PMID: 27376248
20. Our results highlight the role of COX-2 in constitutive IDO1 expression by human tumors and substantiate the use of COX-2 inhibitors to improve the efficacy of cancer immunotherapy, by reducing constitutive IDO1 expression, which contributes to the lack of T-cell infiltration in "cold" tumors, which fail to respond to immunotherapy PMID: 28765120
21. this data we have identified the structure of two possible compounds that may be even more potent pharmacological repressors of IDO-1. PMID: 28735627
22. Prognostic value of IDO in acute myeloid leukemia. PMID: 26762931
23. These findings indicate that IDO1 has the potential to participate in or contribute to the formation of new capillaries, supporting the applicability of IDO1-targeting molecular therapy in lung cancer. PMID: 28498425
24. induces PD-L1 expression by melanoma cells PMID: 27121174
25. PD-L1, IDO-1, and B7-H4 are differentially expressed in human lung carcinomas and show limited co-expression. While PD-L1 and IDO-1 are associated with increased tumor-infiltrating lymphoycte and IFN-gamma stimulation, B7-H4 is not. PMID: 27440266
26. Data show that indole 23-dioxygenase (IDO) is variably expressed by tumor-infiltrating immune cells or reactive cells rather than lymphoma cells in diffuse large-cell lymphoma (DLBCL). PMID: 26727587
27. High IDO1 expression is associated with hepatocellular carcinoma. PMID: 26895379
28. Epacadostat significantly decreases Treg proliferation induced by IDO production from IFN-gamma plus LPS matured human DCs, although the Treg phenotype does not change PMID: 27192116
29. Inhibition of TOR serine-threonine kinases (mTOR) strongly induced indoleamine 23-dioxygenase 1 (IDO1) expression and activity, corroborating its ability to recruit Treg cells in the tumor microenvironment. PMID: 27174915
30. up-regulation induces immunosuppression and may enhance the risk of hepatitis C virus and hepatitis B virus induced hepatocellular carcinoma [review] PMID: 28428708
31. MALAT1-overexpressed MSCs promoted M2 macrophage polarization and this effect was mediated by MALAT1-induced IDO expression, suggesting that MALAT1 may enhance the immunosuppressive properties of MSCs in vivo. PMID: 28176360
32. this study demonstrated that the downregulation of IDO expression on the endothelial cells of the villous stroma was associated with preeclampsia PMID: 28131097
33. The data suggest that in Puumala infection, the mechanism responsible for the suppressive effect of IDO is not metabolic control of effector cells but rather the signaling mediated by tryptophan breakdown products, such as kynurenine. PMID: 28057727
34. These results show IDO is upregulated with RSV infection and this upregulation likely participates with IFN-gamma in inhibition of virus replication and suppression of some host cell responses to infection. PMID: 28963880
35. IDO role in cancer immune responses [review] PMID: 26517538
36. This study demonstrated that IDO-1 was elevated in the Solid Component and Cyst Fluid of Human Adamantinomatous Craniopharyngioma. PMID: 28859336
37. The Binding Mode of N-Hydroxyamidines to Indoleamine 2,3-Dioxygenase 1 PMID: 28731684
38. Fourier transform infrared (FTIR) and nanosecond time-resolved optical spectroscopy to hIDO1 variants with modified heme pocket structures to identify important amino acid residues that stabilize the substrate in the active site. PMID: 28189796
39. Thus, our study provides novel insights into the interaction between a small molecule inhibitor INCB14943 and IDO1 protein. The structural information will facilitate future IDO1 inhibitor design. PMID: 28412361
40. The aim of the present study was to clarify the effect of IDO1induced macrophages on the growth of endometrial stromal cells in endometriosis. IDO1 educated-macrophages may facilitate the survival of retrograde endometrial tissues, and be involved in the pathogenesis of endometriosis. PMID: 28260094
41. Our results identified FGL2, GAL, SEMA4D, SEMA7A, and IDO1 as new candidate genes that could be involved in MSCs-mediated immunomodulation. FGL2, GAL, SEMA4D, SEMA7A, and IDO1 genes appeared to be differentially transcribed in the different MSC populations. Moreover, these genes were not similarly modulated following MSCs-exposure to inflammatory signals PMID: 28336906
42. IDO mediated conversion of FOXP3 -T cells to Tregs predominantly occurs in children with inflammatory bowel disease. PMID: 28337881
43. The study confirmed that high IDO expression in pancreatic adenocarcinoma was related to poor prognosis of patients. These findings provided evidence that IDO was involved in pancreatic adenocarcinoma progression and might serve as a relevant therapeutic target. PMID: 28303855
44. ver-expression of CTLA4 and IDO1 was significantly associated with biochemical recurrence. Our results provide clues on the mechanisms of tumor development and point to potential biomarkers for early detection and treatment for prostate cancer in young men. PMID: 28027300
45. IDO1 mRNA expression in cervical cancer PMID: 27761872
46. Structural Study of a Flexible Active Site Loop in Human Indoleamine 2,3-Dioxygenase and Its Functional Implications. PMID: 27112409
47. IDO gene expression is a feature of aggressive non-muscle-invasive urothelial cell bladder carcinoma, suggesting a potential immunosuppressive role of IDO PMID: 28314306
48. Data show that in the absence of indoleamine 2,3-dioxygenase (IDO) inhibition, fatty acid oxidation increased along with increased activity of carnitine palmitoyltransferase I (CPT1). PMID: 27667153
49. High IDO1 expression is associated with cervical cancer. PMID: 27106797
50. PSG stimulated IDO activity under the conditions of induction of the monocytes by interferon-gamma. PMID: 27595833
顯示更多

收起更多
亞細胞定位：

Cytoplasm, cytosol.
蛋白家族：

Indoleamine 2,3-dioxygenase family
組織特異性：

Expressed in mature dendritic cells located in lymphoid organs (including lymph nodes, spleen, tonsils, Peyers's patches, the gut lamina propria, and the thymic medulla), in some epithelial cells of the female genital tract, as well as in endothelial cell
數據庫鏈接：

HGNC: 6059

OMIM: 147435

KEGG: hsa:3620

STRING: 9606.ENSP00000430505

UniGene: Hs.840

Most popular with customers

YWHAG Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
YWHAB Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF, FC

Species Reactivity: Human, Mouse, Rat
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
XPNPEP1 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
XDH Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WT1 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WFDC2 Recombinant Monoclonal Antibody

Applications: ELISA, WB, FC

Species Reactivity: Human
WDR5 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human