HNRNPC Recombinant Monoclonal Antibody
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中文名稱：HNRNPC重組抗體
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貨號(hào)：CSB-RA010605A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, JK whole cell lysate, Raji whole cell lysate, MCF7 whole cell lysate
All lanes: HNRNPC antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 33, 36, 28 kDa
Observed band size: 42 kDa -
IHC image of CSB-RA010605A0HU diluted at 1:300 and staining in paraffin-embedded human braintissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody -
IHC image of CSB-RA010605A0HU diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody -
IHC image of CSB-RA010605A0HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody -
Immunofluorescence staining of Hela cell with CSB-RA010605A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Immunofluorescence staining of HepG2 cell with CSB-RA010605A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing MCF7 cells stained with CSB-RA010605A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was Rabit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. -
Immunoprecipitating HNRNPC in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA010605A0HU ?in?Hela?whole?cell?lysate.? Lane 2:CSB-RA010605A0HU（3μg）+ Hela whole cell lysate（500μg）
Lane 3: Hela whole cell lysate(20μg)
For western blotting, Goat polyclonal to rabbit IgG antibody was used as the secondary antibody (1/50000)
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CSB-RA010610A0HU HNRNPC重組單克隆抗體是針對(duì)異質(zhì)核核糖核蛋白C（HNRNPC）研發(fā)的高特異性檢測(cè)工具。該蛋白作為RNA結(jié)合蛋白家族成員，廣泛參與mRNA前體剪接、轉(zhuǎn)錄后調(diào)控及RNA穩(wěn)定性維持等關(guān)鍵生物學(xué)過程，其異常表達(dá)與腫瘤發(fā)生、神經(jīng)退行性疾病等病理機(jī)制密切相關(guān)。本抗體經(jīng)ELISA、免疫印跡、免疫組化、免疫熒光、流式細(xì)胞術(shù)及免疫沉淀等多平臺(tái)嚴(yán)格驗(yàn)證，在不同實(shí)驗(yàn)體系中均展現(xiàn)出優(yōu)異的靶標(biāo)識(shí)別能力：免疫印跡推薦使用1:500-1:5000稀釋度，可清晰識(shí)別目標(biāo)蛋白；免疫組化（1:50-1:200）能準(zhǔn)確顯示組織樣本中的蛋白定位；免疫熒光（1:20-1:200）適用于亞細(xì)胞水平可視化分析；免疫沉淀（1:200-1:1000）可有效富集目標(biāo)分子進(jìn)行互作研究。該產(chǎn)品適用于探索RNA代謝調(diào)控、基因表達(dá)網(wǎng)絡(luò)及疾病相關(guān)分子機(jī)制等科研領(lǐng)域，特別是在腫瘤異常RNA加工、神經(jīng)退行性病變的轉(zhuǎn)錄后調(diào)控等研究中具有重要應(yīng)用價(jià)值。嚴(yán)格的生產(chǎn)質(zhì)控確保批次間高一致性，為復(fù)雜實(shí)驗(yàn)體系提供穩(wěn)定可靠的支持。
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Uniprot No.：
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基因名：HNRNPC
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別名：C1 antibody; C2 antibody; Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody; Heterogeneous nuclear ribonucleoprotein C antibody; Heterogeneous nuclear ribonucleoproteins C1/C2 antibody; HNRNP antibody; hnRNP C1 / hnRNP C2 antibody; hnRNP C1/C2 antibody; Hnrnpc antibody; HNRPC antibody; HNRPC_HUMAN antibody; MGC104306 antibody; MGC105117 antibody; MGC117353 antibody; MGC131677 antibody; Nuclear ribonucleoprotein particle C1 protein antibody; Nuclear ribonucleoprotein particle C2 protein antibody; SNRPC antibody
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反應(yīng)種屬：Human
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免疫原：A synthesized peptide derived from human HNRNPC
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號(hào)：9G1
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF, FC, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:200-1:500 IF 1:20-1:200 IP 1:200-1:1000 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing.
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基因功能參考文獻(xiàn)：
- APP levels then decrease progressively as a function of age in close relationship with the gradual normalization of FMRP and hnRNP C levels. PMID: 26048669
- Endoplasmic reticulum resident chaperone GRP78, mitochondrial protein Prohibitin and heterogeneous nuclear ribonucleoprotein hnRNPC (C1/C2) have been shown to interact with viral RNA. Hence it is proposed that these are the principle candidates governing endoplasmic reticulum stress-induced apoptosis in JEV infection. PMID: 28102850
- Our results indicate that a balance between lncRNA SNHG1 and hnRNPC regulates p53 activity and p53-dependent apoptosis upon doxorubicin treatment, and further indicate that a change in lncRNA subcellular localization under specific circumstances is biologically significant. PMID: 28264987
- These data suggest an interplay between CELF2 and hnRNP C as the mechanistic basis for activation-dependent alternative splicing of TRAF3 exon 8. PMID: 28031331
- These data indicate that hnRNPC1/C2 binds to both DNA and RNA and influences both gene expression and RNA splicing, but these actions do not appear to be linked through 1,25(OH)2D-mediated induction of transcription. PMID: 27672039
- These findings suggest that hnRNP C1/C2 is involved in dengue virus replication at the stage of viral RNA synthesis. PMID: 25890165
- HnRNP C, YB-1 and hnRNP L coordinately enhance skipping of human MUSK exon 10 to generate a Wnt-insensitive MuSK isoform. PMID: 25354590
- Our results suggested that hnRNP C1 controls HPV16 late gene expression. PMID: 25878250
- m(6)A-switch-regulated HNRNPC-binding activities affect the abundance as well as alternative splicing of target mRNAs, demonstrating the regulatory role of m(6)A-switches on gene expression and RNA maturation PMID: 25719671
- PTBP1 and hnRNP C repress exon 3 inclusion, and that downregulation of PTBP1 inhibited BIM-mediated apoptosis. PMID: 24743263
- down-regulation of MALAT-1 expression compromised the cytoplasmic translocation of hnRNP C in the G2/M phase and resulted in G2/M arrest PMID: 23973260
- Heterogeneous nuclear ribonucleoprotein C is a key regulator of BRCA gene expression and homologous recombination-based DNA repair. PMID: 23585894
- By preventing U2AF65 binding to Alu elements, hnRNP C plays a critical role as a genome-wide sentinel protecting the transcriptome. PMID: 23374342
- The data indicated that hnRNPC controls the aggressiveness of glioblastoma cells through the regulation of PDCD4. Silencing of hnRNPC lowered miR-21 levels, in turn increasing the expression of PDCD4, suppressing Akt and p70S6K activation. PMID: 22907752
- p27kip1 upregulated by hnRNPC1/2 antagonizes CagA-mediated pathogenesis. PMID: 22404445
- study shows mechanism by which Pol II transcripts are classified according to length; heterotetramer of hnRNP C1/C2 measures the length of the transcripts like a molecular ruler, by binding to unstructured RNA regions longer than 200 to 300 nucleotides PMID: 22461616
- One of the identified proteins, hnRNPC, was found to interact with small hepatitis delta virus antigen in vitro and in vivo in human liver cells. PMID: 21774814
- Studies indicated that DDX21, HNRNPC, and RCC2 were isolated from Ku86 multicomponent complex in response to DNA damage. PMID: 20873769
- HNRNP C recognizes uridine tracts with a defined long-range spacing consistent with HNRNP particle organization. PMID: 20601959
- FMRP represses translation by recruiting APP mRNA to processing bodies, whereas hnRNP C promotes APP translation by displacing FMRP, thereby relieving the translational block. PMID: 20473314
- The authors report here that the 5' end of poliovirus negative-strand RNA is capable of interacting with endogenous hnRNP C, as well as with poliovirus nonstructural proteins. PMID: 20164237
- The authors propose that hnRNP C interacts with poliovirus RNA and replication proteins to increase the efficiency of viral genomic RNA synthesis. PMID: 20189623
- phosphorylation of heterogeneous nuclear ribonucleoprotein C1/C2 in response to physiologic levels of hydrogen peroxide in endothelial cells PMID: 11877401
- nuclear ribonucleoproteins C1 and C2 are part of the RNP complex that forms on XIAP IRES, the cellular levels of hnRNPC1 and -C2 parallel the activity of XIAP IRES and the overexpression of hnRNPC1-C2 specifically enhanced translation of XIAP IRES PMID: 12482981
- hnRNP C, via internal ribosomal entry site binding, modulates translation of c-myc mRNA in a cell cycle phase-dependent manner PMID: 12509468
- the acidic C-terminal domain of hnRNP-C1/C2 could be a regulatory domain and may play an important role in the regulation of mRNA binding by hnRNP-C1/C2. PMID: 12564933
- a novel nuclear export is activated by the ROCK signaling pathway to exclude hnRNP C1/C2 from nucleus, by which the compartmentalization of specific hnRNP components is disturbed in apoptotic cells PMID: 15494373
- CK1alpha-mediated phosphorylation modulates the mRNA binding ability of hnRNP-C PMID: 15687492
- findings suggest that hnRNP C1 has a role in positive-strand RNA synthesis in poliovirus-infected cells, possibly at the level of initiation PMID: 15731220
- upstream element in human papillomavirus type 16 interacted specifically with CstF-64, hnRNP C1/C2 & polypyrimidine tract binding protein, suggesting these factors were enhancing or regulating polyadenylation at the HPV-16 early polyadenylation signal PMID: 15767428
- Direct in vivo interaction of hnRNPC with the urokinase receptor mRNA 3'UTR was demonstrated. PMID: 16010978
- Results describe the purification of a locus control region-associated remodeling complex consisting of heterogeneous nuclear ribonucleoprotein C1/C2, SWI/SNF, and MeCP1 as a single homogeneous complex. PMID: 16217013
- These results demonstrate that hnRNP C1/C2 is involved in maintenance of cellular homeostasis besides cellular differentiation and proliferation. PMID: 16960656
- in addition to its RNA-processing functions, hnRNP C1/C2 may be a key determinant of the temporal patterns of VDRE occupancy PMID: 17071612
- regulation of IRES-mediated translation by hnRNP C1/C2 and Unr might be important in mitosis PMID: 17159903
- A novel cis-element in the 5' coding region of p53 mRNA and its interaction with heterogeneous nuclear ribonucleoprotein (hnRNP)C1/C2, is described. PMID: 18296503
- Intracellular NS1 protein of dengue virus interacts with hnRNP C1/C2. PMID: 18471994
- The RNA binding domain of heterogeneous nuclear ribonuclear protein C binds and stabilizes urokinase-type plasminogen activator receptor (uPAR) mRNA, attributing the key role of this domain in hnRNPC-mediated uPAR regulation in lung epithelial cells. PMID: 18494499
- The H(2)O(2) responsive pre-mRNA binding protein hnRNP-C is up-regulated in atherosclerosis. PMID: 18508286
- anti-hnRNP C1/C2 antibody hampered splicing of SMN1 exon 7, but did not affect splicing of SMN2 exon 7 PMID: 19628962
- endogenous hnRNP C and PTEN interact and co-localize within the nucleus PMID: 19740742
- This protein has been found differentially expressed in the Wernicke's Area from patients with schizophrenia. PMID: 19405953
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亞細(xì)胞定位：Nucleus. Note=Component of ribonucleosomes.
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蛋白家族：RRM HNRPC family, RALY subfamily
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