GRIA2 Recombinant Monoclonal Antibody

說明書

中文名稱：

GRIA2重組抗體
貨號：

CSB-RA009899A0HU
規(guī)格：

￥1320
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA009899A0HU GRIA2重組單克隆抗體是針對谷氨酸受體AMPA亞基2（GRIA2）開發(fā)的科研用檢測工具。該抗體特異性識別GRIA2蛋白，該靶點(diǎn)作為中樞神經(jīng)系統(tǒng)興奮性突觸傳遞的核心調(diào)控分子，在突觸可塑性形成及認(rèn)知功能中發(fā)揮重要作用，其表達(dá)異常與阿爾茨海默病、癲癇等神經(jīng)疾病密切相關(guān)。經(jīng)ELISA方法驗(yàn)證，本產(chǎn)品具有高親和力和批次穩(wěn)定性，可精準(zhǔn)識別天然構(gòu)象及重組表達(dá)的GRIA2蛋白，適用于體外實(shí)驗(yàn)體系中靶蛋白的定性與半定量分析。推薦應(yīng)用于神經(jīng)元培養(yǎng)模型、腦組織樣本的受體定位研究，以及神經(jīng)退行性疾病分子機(jī)制相關(guān)的蛋白質(zhì)相互作用分析，為探索GRIA2在突觸信號轉(zhuǎn)導(dǎo)、神經(jīng)網(wǎng)絡(luò)調(diào)控及病理狀態(tài)下的功能變化提供可靠檢測支持。本產(chǎn)品需配合標(biāo)準(zhǔn)實(shí)驗(yàn)方案使用，建議通過預(yù)實(shí)驗(yàn)優(yōu)化檢測條件。
Uniprot No.：

P42262
基因名：

GRIA2
別名：

AMPA 2 antibody; AMPA selective glutamate receptor 2 antibody; AMPA-selective glutamate receptor 2 antibody; AMPA2 antibody; GluA2 antibody; GLUR 2 antibody; GLUR B antibody; GluR K2 antibody; GluR-2 antibody; GluR-B antibody; GluR-K2 antibody; GLUR2 antibody; GLURB antibody; Glutamate receptor 2 antibody; Glutamate receptor ionotropic AMPA 2 antibody; Glutamate receptor ionotropic antibody; Gria2 antibody; GRIA2_HUMAN antibody; HBGR2 antibody
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from human GRIA2
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

4C7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA
Protocols：

ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system. It plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting.
基因功能參考文獻(xiàn)：
1. This review showed that the mutation GR1A protein associate with Obsessive-compulsive disorder. PMID: 28608743
2. Measured the expression of GRIA2 and GABRA1 in patients with methamphetamine-use disorder. Also examined whether miR-181a down-regulates GRIA2 and GABRA1 in a cell-based assay. We further examined the effects of chronic methamphetamine exposure on the expression of miR-181a, GRIA2 and GABRA1. The results demonstrated that serum GRIA2 is higher in patients with methamphetamine-use disorder than in healthy controls. PMID: 27767084
3. This study demonstrated that a significant decrease in the protein level of GluN2A in major depression disorder. PMID: 27661418
4. both the intracellular C-terminal domain (CTD) and the loop region between the M1 and M2 helices move during activation and the CTD is detached from the membrane PMID: 27313205
5. The results of this study suggest that neurons in hypothalamic hamartoma may bear Ca(2+) -permeable AMPA receptors(GluA2 ) due to dislocation of ADAR2 PMID: 28195308
6. A transient positive feedback mechanism between AMPAR and stargazin has implications for information processing in the brain, because it should allow activity-dependent facilitation of excitatory synaptic transmission through a postsynaptic mechanism. PMID: 26744192
7. The GluR2 subunit of the AMPA receptor is involved in cell migration and calcium signaling. PMID: 26311781
8. RAB39B selectively regulates GluA2 trafficking to determine synaptic AMPAR composition PMID: 25784538
9. GRIA2*CCC polymorphism is genetic risk marker for paranoid schizophrenia in Russians.Low risk genetic markers of paranoid schizophrenia were revealed: in Tatars-GRIA2*T/T (rs43025506) of GRIA2 gene and GRIA2*CCT in Russians. PMID: 25842862
10. GRIA2 is a useful marker for distinguishing solitary fibrous tumour from most mimics PMID: 24456377
11. a link between neurodegenerative processes and deficient RNA editing of the GluA2 Q/R site. PMID: 24679603
12. Statistical analysis showed no association between migraine and the GRIA2 and GRIA4 polymorphisms investigated. PMID: 24512576
13. analysis of changes in receptor kinetics with the R628E charge-inverting mutation in the "linker" region of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor PMID: 24550387
14. We show that excitatory cortical-patterned neurons derived from multiple human pluripotent stem cell lines exhibit native-like maturation changes in AMPAR composition such that there is an increase in the expression of GluA2(R) subunits. PMID: 24623784
15. The data potentially suggest a lack of epistatic interaction between GRIA2 and GRIA4 variants regarding clinical outcomes in patients with major depressive disorder. PMID: 23613500
16. The ionotrophic glutamate receptors AMPA2 and AMPA3 were decreased in hippocampus in patient with multiple sclerosis. PMID: 23595422
17. ADAR2 expression level reflects editing activity at the GluA2 Q/R site; although the edited GluA2 pre-mRNA is readily spliced, the unedited GluA2 pre-mRNA is also spliced and transported to the cytoplasm when ADAR2 expression is low. PMID: 22366356
18. Did not observed significant association between GRIA2 polymorphisms and clinical improvement in patients with Major depressive disorder.But rs4302506 and rs4403097 single nucleotide polymorphisms could be associated with age of onset of the disease. PMID: 22057216
19. We demonstrated that the expression of GRIA2 among the differentially expressed genes provides better prognosis of patients with advanced serous papillary ovarian adenocarcinoma. PMID: 22644307
20. The balance of expression of nerve growth factor (NGF) and that of its receptors shifts toward cell death mechanisms during the progression of Alzheimer disease. PMID: 21397006
21. the GluR2 gene is embedded into an open chromatin configuration in glioma cells and expression of GluR2 is controlled by REST and Sp1 PMID: 21948504
22. SNPs within GRIA2 may not be associated with the development and treatment outcomes in BD PMID: 22122651
23. significant association GRIA2 polymorphisms was found with the diagnosis of schizophrenia. PMID: 22094384
24. We conclude that polymorphisms in the GluR2 gene (GRIA2) are not a major contributory factor in the pathogenesis of ALS. PMID: 20409611
25. Data show that CALM influences the cell surface level of the AMPA receptor subunit GluR2. PMID: 21221849
26. Gain-of-function glutamate receptor interacting protein 1 variants alter GluA2 recycling and surface distribution in patients with autism PMID: 21383172
27. Transsynaptic signaling mediated by the extracellular domain of GluR2 regulates the stability of presynaptic terminals. PMID: 21173224
28. [review] The importance of mGlu2/3 glutamate receptors during normal central nervous system development is likely to play an important mechanistic role in the neurodevelopmental hypothesis of schizophrenia. PMID: 19933774
29. Post-transcriptional editing of this subunit at the Q/R/N site controls calcium permeability PMID: 11860506
30. role of interaction with N-ethylmaleimide-sensitive factor in regulation of AMPA receptors by brain-derived neurotrophic factor PMID: 12130635
31. Low levels of GluR2 mRNA in motoneurons of ALS did not differ from the control group, implying that selective reduction of the GluR2 subunit cannot be a mechanism of AMPA receptor-mediated neurotoxicity in ALS PMID: 12694394
32. the Q/R site of GluRs editing is regulated in a regional manner and the GluR2 Q/R site editing is critically regulated by ADAR2 in human brain PMID: 12859334
33. GluR2, a subunit of a ligand-gated cation channel, is up-regulated in leiomyomata relative to myometrium by 15- to 30-fold at the protein and mRNA level and is localized in endothelial cells PMID: 14630051
34. There is a defect in the editing of the mRNA encoding the GluR2 subunit of glutamate AMPA receptors in the spinal motor neurons of individuals affected by amyotrophic lateral sclerosis PMID: 14985749
35. R/G editing status: the relative amount of edited GLUR2 mRNA was increased in epileptic hippocampi, whereas no changes were found in neocortical tissues. PMID: 15006707
36. Control of GLUR2 translation was mediated by a sequence containing a 34-42 nucleotide imperfect GU repeat predicted to form secondary structure in vivo. This translation suppression domain is included in some rat and human GluR2 transcripts in vivo PMID: 15071096
37. GluR2 was localized to the perikarya and proximal dendrites of BLA neurons; dense labeling was also present over the pyramidal cell layer of hippocampal subfields CA1 and CA3 PMID: 16045445
38. Circumstance data have indicated that the GluR2 subunits dictate Ca2+/Zn2+ permeability of AMPA receptor channels and gate injurious Ca2+/Zn2+ signals in vulnerable neurons. PMID: 16215279
39. Laser capture microdissection combined with quantitative PCR was used to examine the expression of AMPA (GRIA1-4) and NMDA (GRIN1, 2A and 2B) subunit mRNA levels in Layer II/III and Layer V pyramidal cells PMID: 17942280
40. Of the three AMPA genes analyzed here, only GRIA3 seems to be involved in the pathogenesis of schizophrenia, but only in females. PMID: 18163426
41. utilized reverse-transcription polymerase chain reaction and BbvI digestion to demonstrate that neural progenitor cells contain Q/R-unedited GluR2, and differentiated cells contain Q/R-edited GluR2 subunits PMID: 18403631
42. the GluR4 subunit exhibits a different correlation between receptor activation and ligand-binding domain cleft closure than does GluR2 PMID: 19102704
43. These findings indicate that the absence of the GluR2 subunit favours malignancy in gliomas PMID: 19558602
44. This dimeric structure provides a mechanism for how the ATDs can drive receptor assembly and subtype-restricted composition. PMID: 19651138
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亞細(xì)胞定位：

Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic density membrane; Multi-pass membrane protein.
蛋白家族：

Glutamate-gated ion channel (TC 1.A.10.1) family, GRIA2 subfamily
數(shù)據(jù)庫鏈接：

HGNC: 4572

OMIM: 138247

KEGG: hsa:2891

STRING: 9606.ENSP00000264426

UniGene: Hs.32763

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