FOS Recombinant Monoclonal Antibody

說明書

中文名稱：

FOS重組抗體
貨號：

CSB-RA008790A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate
All lanes: c-FOS antibody at 0.81μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 41, 29, 37 KDa
Observed band size: 62 KDa

IHC image of CSB-RA008790A0HU diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-RA008790A0HU diluted at 1:81 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-RA008790A0HU at 1:27, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Overlay histogram showing Hela cells stained with CSB-RA008790A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

FOS Recombinant Monoclonal Antibody（Code: CSB-RA008790A0HU）是靶向原癌基因FOS的高特異性抗體。FOS蛋白作為AP-1轉錄因子復合體的核心成員，廣泛參與細胞增殖、分化及應激響應等關鍵生物學過程，其異常表達與多種病理機制密切相關。該抗體通過重組技術制備，經ELISA、免疫印跡（WB）、免疫組化（IHC）、免疫熒光（IF）及流式細胞分析（FC）等多平臺驗證，表現出優異的一致性。實驗推薦稀釋比例為：WB 1:500-1:5000，IHC 1:50-1:200，IF 1:20-1:200，能夠精準識別天然構象的FOS蛋白，并在多種樣本類型中呈現低背景和高信噪比。其應用涵蓋腫瘤發生機制研究、細胞周期調控分析以及基因表達信號通路探索等領域，適用于體外培養細胞、組織切片等實驗體系，為分子生物學及疾病相關研究提供可靠工具。本產品嚴格質量控制，確保批次間穩定性，適用于科研級實驗需求。
Uniprot No.：

P01100
基因名：

FOS
別名：

Activator protein 1 antibody; AP 1 antibody; C FOS antibody; Cellular oncogene c fos antibody; Cellular oncogene fos antibody; FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody; FBJ murine osteosarcoma viral oncogene homolog antibody; FBJ murine osteosarcoma viral v fos oncogene homolog antibody; FBJ Osteosarcoma Virus antibody; FOS antibody; FOS protein antibody; FOS_HUMAN antibody; G0 G1 switch regulatory protein 7 antibody; G0/G1 switch regulatory protein 7 antibody; G0S7 antibody; Oncogene FOS antibody; p55 antibody; proto oncogene c Fos antibody; Proto oncogene protein c fos antibody; Proto-oncogene c-Fos antibody; v fos FBJ murine osteosarcoma viral oncogene homolog antibody
反應種屬：

Human, Mouse
免疫原：

A synthesized peptide derived from human FOS
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

14C10
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
基因功能參考文獻：
1. Findings iindicate a human bone tumour defined by mutations of FOS and FOSB. PMID: 29858576
2. gammadelta T cells suppressed iDCs osteoclastogenesis by downregulation of the RANK/cFos/ATP6V0D2 signaling pathway. PMID: 30066839
3. Mutant cellular AP-1 proteins promote expression of a subset of Epstein-Barr virus late genes in the absence of lytic viral DNA replication. PMID: 30021895
4. Low c-fos expression is associated with Oral Squamous Cell Carcinoma. PMID: 29582647
5. Study demonstrated that c-Fos was highly expressed in most of ovarian epithelial carcinoma cases and was significantly correlated with Lewis y. Also, the results revealed that c-Fos interacted with the FUT1 promoter. Silencing of c-Fos prevented TGF-beta1-induced Lewis y expression. PMID: 29130097
6. These findings indicate that the c-Fos/miR-22/MDC1 axis plays a relevant role in DNA repair in terminally differentiated cells, which may facilitate our understanding of molecular mechanism underlying the downregulating DNA repair in differentiated cells. PMID: 28637007
7. our results strongly suggest a novel role of c-Fos as a regulator of epithelial-mesenchymal transition and cancer stem cell(CSC) reprogramming in Head and neck squamous cell carcinoma (HNSCC)cells, which may hold potential as a CSC-directed therapeutic approach to improve HNSCC treatment PMID: 27965308
8. High c-fos expression is associated with malignant glioma. PMID: 27602752
9. Immunohistochemistry was employed to analyze cFos, cJun and CD147 expression in 41 UCB cases and 34 noncancerous human bladder tissues. PMID: 28358415
10. data enforced the evidence that knockdown of c-Fos inhibited cell proliferation, migration, and invasion, and promoted the apoptosis of OS cells accompanied by altered expression of Wnt2 and Fzd9 PMID: 28665975
11. These findings demonstrate an essential role for the ERK pathway together with c-JUN and c-FOS in the differentiation activity of LukS-PV. PMID: 27102414
12. novel function of KDM2B in the negative regulation of cell proliferation by assembling an E3 ligase to targeting c-Fos protein degradation that is antagonized by mitogenic stimulations PMID: 26725323
13. NF-Y Binding Site Architecture Defines a C-Fos Targeted Promoter Class PMID: 27517874
14. c-fos underexpression is associated with Myelodysplastic Syndrome. PMID: 27513856
15. miR-101 is downregulated in bladder cancer cells and has an inhibitory role in the regulation of bladder cancer cell proliferation and invasion via directly targeting c-FOS. PMID: 27485165
16. We found that c-jun or c-fos was significantly associated with lymph node metastasis, and coexpression of c-jun/c-fos, or c-jun/c-fos/p53 were significantly associated with lymph node metastasis, poor differentiation and clinical stage. PMID: 27558649
17. CRAC channel blockade also suppressed Oxo-M-induced c-fos and interleukin-2 expression PMID: 27474128
18. The results indicate that 17beta-estradiol-induced endometrial stromal cell invasion is dependent on c-fos-mediated MMP-9 expression. PMID: 26917263
19. FOS is a downstream effector of high glucose stimulation in peritoneal mesothelial cells that contributes to TGF-beta1 production. PMID: 26018137
20. VEGF-induced endothelial migration is mediated primarily by induction of JunB whereas the promotion of endothelial proliferation by VEGF is mediated by JunB-independent AP-1 family members. PMID: 26860974
21. c-Fos can protect against HDAC3 neurotoxicity. PMID: 25592718
22. These results indicate that IL-17A enhances COX2 expression and PGE2 production via the p38/c-Fos and JNK/c-Jun signalling pathways in NP cells to mediate intervertebral disc inflammation. PMID: 26988982
23. the results of this study suggest that FOS is among the candidate genes of schizophrenia and that changes in the expression of c-Fos protein may contribute to molecular mechanisms of schizophrenia-related alterations in synaptic plasticity. PMID: 25706621
24. Increased c-Fos expression is through TRPM3-mediated stimulation of the c-Fos promoter. PMID: 26493679
25. A novel AP-1 binding site at -1363 bp of the human TF promoter region was identified. PMID: 26631725
26. Simultaneous high expression of ID1 and c-Jun or c-Fos was correlated with poor survival in esophageal squamous cell carcinoma patients. PMID: 26858249
27. miR-146a has a role in targeting Fos expression in human cardiac cells PMID: 26112171
28. The translocation causes truncation of the FOS protein, with loss of the transactivation domain, which is thereby a novel mechanism involved in tumorigenesis. PMID: 26173738
29. ERK1 and ERK2 regulated the expression of c-Fos and c-Jun proteins in human cervical cancer cells. PMID: 25647783
30. O-GlcNAcylation of MLL5beta at T440 residue is critical for MLL5 recruitment to the HPV16/18-long control region through its interaction with AP-1. PMID: 25670814
31. The RNA binding complexes NF45-NF90 and NF45-NF110 associate dynamically with the c-fos gene and function as transcriptional coactivators. PMID: 26381409
32. Data show that interleukin-1 receptor type 2 (IL1R2) forms a complex with c-Fos proto-oncogene protein and activates the interleukin-6 (IL-6) and vascular endothelial growth factor A (VEGF-A) promoters. PMID: 26209639
33. Data indicate that deregulation of transcription factor AP-1 and microRNA-21-mediated axis led to an enhanced cell growth in hepatocellular carcinoma (HCC). PMID: 25544773
34. These results establish c-Fos homodimers as a novel form of the AP-1 complex that may be an autonomous transcription factor in c-Fos-overexpressing tissues and could contribute to tumor development. PMID: 26303532
35. Endoplasmic reticulum stress activates the hepatic AP-1 complex via MAPK-dependent signaling pathways. PMID: 25077945
36. co-expression of c-Fos or Fra1 was able to cooperate with TAp73 in potentiating cellular growth, similarly to c-Jun. These data together suggest that TAp73 plays a vital role in activation of AP-1 target genes via direct binding to c-Jun PMID: 26018080
37. The light-induced FOS response in melanopsin expressing HEK-293 cells is correlated with melanopsin quantity and dependent on light duration and irradiance. PMID: 24909488
38. c-Fos promotes the progression of viral transcription from early to late stages and accelerates viral lytic replication upon sustained ORF45-ERK-RSK activation during the Kaposi's Sarcoma-Associated Herpesvirus lytic life cycle. PMID: 25903346
39. By targeting the proto-oncogene Fos, miR-101 is involved in G1-to-S phase transition in cervical cancer cells in vitro. PMID: 24987920
40. Data suggest that p38 MAP kinase regulates c-Fos/cellular oncogene fos mRNA stability/decay by affecting state of phosphorylation of ELAVL1/HuR (Hu antigen R). PMID: 25588078
41. CDK12 plays an important role in cotranscriptional processing of c-FOS transcripts PMID: 25384976
42. We found significant negative correlations regarding the expression of the genes COMT, MAOB, DRD4, DRD5 and FOS, indicating that increased schizotypy coincides with higher levels of dopaminergic dysregulation on the mRNA-level. PMID: 24630741
43. results support the proposal that cooperative signaling of both NF-kappaB and AP1 (via p38alpha) amplifies STIM1 expression in ECs and, thereby, contributes to the lung vascular hyperpermeability response during sepsis PMID: 25016017
44. SMAR1 has a role in repressing c-Fos-mediated HPV18 E6 transcription through alteration of chromatin histone deacetylation PMID: 25157104
45. This study indicates that increased expression of c-Fos, p-c-Jun, members of AP-1 transcriptional factor and p-JNK is associated with neuronal degeneration in the ganglion cell layer of retinas in diabetic patients. PMID: 24073601
46. S100A4, FOS and CXCR4, playing a major role in tumor progression and metastasis, are downregulated by sorafenib. PMID: 24378831
47. the IL-1beta/p38/AP-1(c-fos)/MMP2 & MMP9 pathway play an important role in metastasis in gastric adenocarcinoma PMID: 24479681
48. the distinct requirement of NF-kappaB for mouse and human c-fos regulation PMID: 24386331
49. c-Fos, a well known AP-1 transcription factor, has emerged as a unique protein with the capacity to associate to specific enzymes of the pathway of synthesis of phospholipids at the endoplasmic reticulum and activate their synthesis. (Review) PMID: 24886961
50. Inflammation mediators act through c-Fos to increase VEGF production in peritoneal mesothelium. PMID: 23760290
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亞細胞定位：

Nucleus. Endoplasmic reticulum. Cytoplasm, cytosol. Note=In quiescent cells, present in very small amounts in the cytosol. Following induction of cell growth, first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum requires dephosphorylation at Tyr-10 and Tyr-30.
蛋白家族：

BZIP family, Fos subfamily
數據庫鏈接：

HGNC: 3796

OMIM: 164810

KEGG: hsa:2353

STRING: 9606.ENSP00000306245

UniGene: Hs.25647

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