FNTB Recombinant Monoclonal Antibody
-
中文名稱：FNTB重組抗體
-
貨號：CSB-RA267691A0HU
-
規(guī)格：￥1320
-
圖片：
-
Western Blot
Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Rat brain tissue
All lanes: FNTB antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49, 44 kDa
Observed band size: 49 kDa -
IHC image of CSB-RA267691A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. -
Immunoprecipitating FNTB in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA267691A0HU in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA267691A0HU(2μg)+ K562 whole cell lysate(500μg)
Lane 3: K562 whole cell lysate (10μg)
-
-
其他：
產(chǎn)品詳情
-
產(chǎn)品描述：CSB-RA267691A0HU FNTB重組單克隆抗體是針對法尼基轉(zhuǎn)移酶β亞基（FNTB）研發(fā)的高特異性檢測工具。FNTB作為法尼基轉(zhuǎn)移酶復(fù)合體的關(guān)鍵催化亞基，通過介導(dǎo)Ras等蛋白的法尼基化修飾參與細胞增殖與信號轉(zhuǎn)導(dǎo)調(diào)控，其異常表達與多種腫瘤發(fā)生發(fā)展密切相關(guān)。本產(chǎn)品經(jīng)多種平臺嚴格驗證：在Western Blot中可清晰識別天然及重組FNTB蛋白，呈現(xiàn)單一特異性條帶；免疫組化實驗顯示其在組織切片中具有精準的亞細胞定位能力；免疫共沉淀驗證證實其能高效富集內(nèi)源性FNTB及其互作蛋白。推薦使用稀釋度覆蓋WB（1:500-5000）、IHC（1:50-200）及IP（1:200-1000）等多種實驗體系，適用于癌癥分子機制研究、蛋白質(zhì)翻譯后修飾分析以及靶向法尼基化通路藥物的體外篩選等科研場景。該抗體具有批次穩(wěn)定性高、交叉反應(yīng)性低的特點，為探索FNTB在細胞信號網(wǎng)絡(luò)中的功能及其病理學(xué)意義提供了可靠工具。
-
Uniprot No.：
-
基因名：FNTB
-
別名：Protein farnesyltransferase subunit beta (FTase-beta) (EC 2.5.1.58) (CAAX farnesyltransferase subunit beta) (Ras proteins prenyltransferase subunit beta), FNTB
-
反應(yīng)種屬：Human, Rat
-
免疫原：A synthesized peptide derived from human FNTB
-
免疫原種屬：Homo sapiens (Human)
-
標(biāo)記方式：Non-conjugated
-
克隆類型：Monoclonal
-
抗體亞型：Rabbit IgG
-
純化方式：Affinity-chromatography
-
克隆號：5H4
-
濃度：It differs from different batches. Please contact us to confirm it.
-
保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
-
產(chǎn)品提供形式：Liquid
-
應(yīng)用范圍：ELISA, WB, IHC, IP
-
推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:50-1:200 IP 1:200-1:1000 -
Protocols：
-
儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
-
貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
-
用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
-
功能：Essential subunit of the farnesyltransferase complex. Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X.
-
蛋白家族：Protein prenyltransferase subunit beta family
-
數(shù)據(jù)庫鏈接：
Most popular with customers
-
-
YWHAB Recombinant Monoclonal Antibody
Applications: ELISA, WB, IHC, IF, FC
Species Reactivity: Human, Mouse, Rat
-
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody
Applications: ELISA, WB, IHC
Species Reactivity: Human
-
-
-
-
-
