EIF5A Recombinant Monoclonal Antibody

說明書

中文名稱：

EIF5A重組抗體
貨號：

CSB-RA932542A0HU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in: A2780 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate
All lanes: EIF5A antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 17, 21 kDa
Observed band size: 18 kDa

IHC image of CSB-RA932542A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of Hela Cells with CSB-RA932542A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Overlay histogram showing Jurkat cells stained with CSB-RA932542A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1*10⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*10⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA932542A0HU EIF5A重組單克隆抗體是針對真核翻譯起始因子5A（EIF5A）開發(fā)的高特異性科研工具。EIF5A作為高度保守的翻譯調(diào)控蛋白，通過獨特的羥腐胺賴氨酸修飾參與核糖體功能調(diào)控，在細胞增殖、凋亡及應(yīng)激反應(yīng)中發(fā)揮關(guān)鍵作用，其異常表達與腫瘤發(fā)生、病毒復(fù)制及神經(jīng)退行性疾病密切相關(guān)。本抗體經(jīng)ELISA、Western Blot、免疫組化（IHC）、免疫熒光（IF）和流式細胞術(shù)（FC）多平臺驗證，推薦稀釋梯度覆蓋WB 1:500-1:5000、IHC 1:50-1:200、IF/FC 1:20-1:200，可精準識別內(nèi)源性EIF5A蛋白。在實驗中能夠清晰呈現(xiàn)不同細胞系及組織樣本中EIF5A的蛋白表達定位，適用于細胞周期調(diào)控機制研究、腫瘤微環(huán)境分析及神經(jīng)細胞功能探索等領(lǐng)域。采用重組蛋白免疫技術(shù)制備，具有批次間高穩(wěn)定性和低交叉反應(yīng)性特征，為研究EIF5A在蛋白質(zhì)翻譯調(diào)控、細胞應(yīng)激響應(yīng)及疾病分子機制中的功能提供了可靠檢測工具。
Uniprot No.：

P63241
基因名：

EIF5A
別名：

Eukaryotic translation initiation factor 5A-1 (eIF-5A-1) (eIF-5A1) (Eukaryotic initiation factor 5A isoform 1) (eIF-5A) (Rev-binding factor) (eIF-4D), EIF5A
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from human eIF5A
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

5E1
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200
FC	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
基因功能參考文獻：
1. EIF5A1 is a vital regulator of epithelial ovarian cancer proliferation and progression. PMID: 29428664
2. Molecular structure of the exportin Xpo4 in complex with RanGTP and the hypusine-containing translation factor eIF5A has been reported. PMID: 27306458
3. the depletion of eIF5A leads to endoplasmic reticulum stress, an unfolded protein response and up-regulation of chaperone expression in HeLa cells. PMID: 27180817
4. Findings indicate that eIF5A-PEAK1-YAP signaling contributes to PDAC development by regulating an STF program associated with increased tumorigenicity. PMID: 28381547
5. eIF5A1 isoform A has a role in mitochondrial function. PMID: 27414022
6. eIF5A-2 protein was highly expressed in gastric cancer tissues PMID: 26282002
7. Upregulation of translation initiation factor 5a observed in thalassemia is a novel finding and plays a protective role toward cell survival under oxidative stress. PMID: 26403856
8. Our results suggest that functional, hypusinated eIF5A is necessary for HIF-1alpha expression during hypoxia and that eIF5A is an attractive target for cancer therapy. PMID: 26773503
9. findings also implicate the eIF5A/RhoA/ROCK module as a potential new therapeutic target to treat metastatic PDAC cells PMID: 26483550
10. eIF5A has been shown to regulate a number of gene products specifically, termed the eIF5A regulon, and its role in translating proline-rich sequences has recently been identified. PMID: 25979826
11. eIF5A proteins utilize PEAK1 as a downstream effector to drive pancreatic ductal adenocarcinoma (PDAC) pathogenesis. PMID: 25261239
12. Mature eIF5A (hypusinated form) is not involved in the autophagic pathway. PMID: 25218134
13. The findings that eIF5A and EF-P are important for specific cellular processes and play a role in the relief of ribosome stalling caused by specific amino acid sequences PMID: 24402910
14. Data suggest a regulatory mechanism for the pro-apoptotic protein eukaryotic translation initiation factor 5A1 (eIF5A1) in which its level is possibly modulated by NF-kappaB in lung cells. PMID: 24491565
15. Data indicate that the signal of EIF5A2, MYCN, and MCL1 genes is decreased in hydroxyurea (HU) and gemcitabine (GEM) treated UACC-1598 ovarian cancer cell line. PMID: 23991020
16. mature eIF5A controls a translational network of cancer-driving genes, termed the eIF5A regulon, at the levels of mRNA abundance and translation PMID: 24220243
17. The expression of eIF5A-2 was up-regulated following EMT phenotype changes in A549 cells, which correlated with enhanced tumor invasion and metastatic capabilities. PMID: 23733422
18. Altered expression of Hsc70 and eIF5A-1 may cause defects in nucleocytoplasmic transport and play a role in esophageal carcinogenesis. PMID: 23539416
19. Overexpression of EIF5A is associated with early-onset colorectal cancer. PMID: 23322277
20. acetylation regulates the subcellular localization of eIF5A2 PMID: 23132580
21. a novel role for miR-331-3p and miR-642-5p in the control of prostate cancer cell growth via the regulation of DOHH expression and eIF5A activity. PMID: 22908221
22. identified PCAF as the major cellular acetyltransferase of eIF5A, and HDAC6 and SIRT2 as its major deacetylases. Inhibition of the deacetylases or impaired hypusination increased acetylation of eIF5A, leading to nuclear accumulation. PMID: 22771473
23. HIV-1 Rev cofactors Sam68, eIF5A, hRIP, and DDX3 also function in the translation of HIV-1 RNA. PMID: 21360055
24. hybrid exercise increases expression of eukaryotic translation initiation factor 5A (EIFSA), peroxisomal biogenesis factor 6 (PEX6) and histone cluster 1 H4 (HIST1H4), compared with electrical stimulation alone PMID: 21778671
25. findings suggested that overexpression of eIF5A-2 correlates with local invasion of NSCLC, and might serve as an adverse prognostic marker of survival for stage I NSCLC patients PMID: 20830705
26. loss of eIF5A activity by this SSAT1-mediated acetylation confirms the strict structural requirement for the hypusine side chain and suggests a possible regulation of eIF5A by hypusine acetylation/deacetylation PMID: 20942800
27. High eIF5A expression is associated with hepatocellular carcinoma. PMID: 19998337
28. These observations collectively indicate that unhypusinated eIF5A1 plays a central role in the regulation of apoptosis. PMID: 20232312
29. These data support the importance of eIF5A and hypusine formation in HIV-1 gene expression. PMID: 19825182
30. Heat stress-induced loss of eukaryotic initiation factor 5A (eIF-5A) in a human pancreatic cancer cell line PMID: 11870779
31. Subcellular distribution of eIF-5A by indirect immunofluorescent staining and by direct visualization of green fluorescent protein tagged eIF-5A (GFP-eIF5A) PMID: 12210765
32. Patients having a higher eIF-5A protein expression showed a relatively poorer survival suggesting the use of eIF-5A as prognostic marker in lung adenocarcinoma. PMID: 12687616
33. EIF-5A expression inhibition by antisense oligodeoxynucleotides significantly enhanced the stimulating effect of GM-CSF on cell growth. Therefore, the eIF-5A accumulation played important roles in the apoptosis induced by UP inhibitors. PMID: 12894223
34. eIF5A may be a regulator of p53, and syntenin might regulate p53 by balancing the regulation of eIF5A signaling to p53 for apoptosis PMID: 15371445
35. These findings suggest that the failure to detect eIF5A-2 protein even in eIF5A-2 mRNA positive cells is, at least in part, due to inefficient translation. PMID: 16519677
36. Recombinant human eIF-5A was crystallized by the hanging-drop vapor diffusion method PMID: 16522190
37. molecular model for the human eIF5A protein based on the crystal structure of the eIF5A from Leishmania brasiliensis PMID: 16842744
38. data suggest that eIF5A mediates important cellular processes like cell viability and senescence through its effects on the stability of certain mRNAs PMID: 16987817
39. analysis of differences in global protein expression in BCR-ABL-positive K562 cells treated with or without imatinib revealed down-regulation of eIF5A; hypusination inhibitors exerted an antiproliferative effect PMID: 17008552
40. These findings collectively indicate that unhypusinated eIF5A may have pro-apoptotic functions and that eIF5A is rapidly translocated to the nucleus following the induction of apoptotic cell death PMID: 17187778
41. analysis of the deoxyhypusine hydroxylase-eukaryotic translation initiation factor (eIF5A) interaction PMID: 17213197
42. Mutational analyses of human eIF5A-1--identification of amino acid residues critical for eIF5A activity and hypusine modification PMID: 18067580
43. Results suggest that the stability of eIF5A may have an important role in determining the fate of the particular cell type after severe heat stress. PMID: 19160416
44. These findings provide strong evidence that the hypusine modification of eIF5A dictates its localization in the cytoplasmic compartment where it is required for protein synthesis. PMID: 19379712
45. Eukaryotic initiation factor 5A (eIF5A) (eIF-4D, eIF-5A) stimulates ribosomal peptidyltransferase activity, transport of HIV-1-mRNAs and binds exportins 1 and 4. Contains hypusine at lys 50. Human EIF5A1 and EIF5A2 encode two isoforms: eIF5AI and eIF5AII. PMID: 11161802
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亞細胞定位：

Cytoplasm. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus, nuclear pore complex. Note=Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
蛋白家族：

EIF-5A family
組織特異性：

Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).
數(shù)據(jù)庫鏈接：

HGNC: 3300

OMIM: 600187

KEGG: hsa:1984

STRING: 9606.ENSP00000336702

UniGene: Hs.104825

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