DNM2 Recombinant Monoclonal Antibody

說明書

中文名稱：

DNM2重組抗體
貨號：

CSB-RA877222A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: HL60 whole cell lysate, Rat brain tissue
All lanes: DNM2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 99, 98 kDa
Observed band size: 99 kDa

Immunofluorescence staining of Hela Cells with CSB-RA877222A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Overlay histogram showing Hela cells stained with CSB-RA877222A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1*10⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*10⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA877222A0HU DNM2重組單克隆抗體是針對動力蛋白2（Dynamin 2）研發的高特異性科研工具，該靶標蛋白在膜運輸、內吞作用及細胞骨架重組等關鍵生物學過程中發揮重要作用。本產品經多種技術平臺驗證，適用于ELISA、蛋白質印跡（WB）、免疫熒光（IF）及流式細胞術（FC）等實驗，其中WB檢測可識別目標蛋白并呈現清晰條帶，推薦使用1:500-1:5000稀釋度；IF和FC實驗分別在1:20-1:200范圍內展現優異的亞細胞定位分析和表面標記檢測能力。抗體經過抗原親和純化及嚴格的免疫原特異性驗證，確保不同批次間的高重復性，適用于神經肌肉疾病機制、腫瘤細胞膜轉運研究以及囊泡運輸相關的基礎科研領域。科研人員可借助該抗體深入探索DNM2在神經退行性疾病、肌營養不良癥或癌細胞侵襲轉移中的分子調控網絡，為相關疾病的分子機制解析提供可靠實驗支持。
Uniprot No.：

P50570
基因名：

DNM2
別名：

Dynamin-2 (EC 3.6.5.5), DNM2, DYN2
反應種屬：

Human, Rat
免疫原：

A synthesized peptide derived from human Dynamin 2
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

1D8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:20-1:200
FC	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis. Regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane.
基因功能參考文獻：
1. DNM2 is a substrate for CDK1-dependent phosphorylation, which plays an important role in the regulation of human sperm acrosomal exocytosis. PMID: 29044420
2. Data found DNM2 highly expressed compared in both forms of ALL and associated with poor prognosis and with tumor cell proliferation. Also, Ikaros directly binds the DNM2 promoter and suppresses its expression. PMID: 27885263
3. a new AHI-1-BCR-ABL-DNM2 protein complex was uncovered, which regulates leukemic properties of these cells through a unique mechanism of cellular endocytosis and ROS-mediated autophagy. Thus, targeting this complex may facilitate eradication of LSCs for curative therapies PMID: 28366933
4. dynamin-2, as a tetramer, might help to establish hemi-fusion and stabilizes the pore during HIV-1 fusion. PMID: 28076788
5. Data suggest DNM2/RRAGB- (or DNM2/RRAGC-)dependent endocytosis of extracellular amino acids (AAs) plays critical role in mTORC1 transport/activation; recruitment of mTORC1 from cytoplasm to lysosome is suppressed by DNM2 inhibition; AA deprivation appears to be main cause of mTORC1 inactivation via DNM2 inhibition. (RHEB = Ras homolog enriched in brain; DNM2 = dynamin II; RRAG = Ras-related GTP binding protein) PMID: 28808055
6. We demonstrate that the dynamin2 effect on T cell adhesion does not involve integrin affinity regulation but instead relies on its ability to modulate integrin valency. PMID: 28273099
7. Thus, dynamin2 contributes to bladder cancer invasion by controlling invadopodia formation in bladder cancer cells and may prove a valuable therapeutic target. PMID: 27771248
8. Study showed that dynamin 2 and cortactin participate in the formation of F-actin bundles, which stabilize filopodia in migrating cancer cells. PMID: 27572123
9. Data demonstrate that dynamin II is required for the E2:ERalpha signaling of physiological functions and uncovers a role for autophagy in the control of ERalpha turnover. PMID: 27009360
10. This study reported the clinical characteristics, molecular diagnosis strategy, and DNM2 gene mutations of four Chinese Han patients with centronuclear myopathy. PMID: 26908122
11. Results demonstrate that overexpression of human DNM2 mRNAs, containing different disease-related mutations, cause a continuum of pathological features in zebrafish similarly to what observed in human centronuclear myopathies and neuropathies. PMID: 26842864
12. uncover a link between the dynamin 2 function and JNK signaling which leads to AP-1 induction PMID: 26475677
13. results provide evidence for a novel Arf6 activation mechanism by Dyn2 through EFA6B and EFA6D in CME in a manner dependent upon the GTPase activity of Dyn2 PMID: 26503427
14. We conclude that DNM2 is a novel negative regulator of NO production in mouse collecting ducts. PMID: 26791826
15. In marked contrast to invadopodia, this degradation does not require the action of Src kinase, Cdc42 or Dyn2. Rather, inhibition of Dyn2 causes a marked upregulation of stromal matrix degradation PMID: 25982272
16. exome sequencing family study reveals that autosomal dominant spastic paraplegia linked to a GTPase-effector domain mutation of dynamin 2 PMID: 26517984
17. This study demonistrated that DNM2 mutation releated to Centronuclear myopathy. PMID: 25957634
18. Dynamin 2 deletion in beta cells caused glucose intolerance and reduced the 2d phase of glucose-stimulated insulin secretion. Dynamin 2 regulates insulin secretory capacity and dynamics in vivo via a mechanism depending on CME and F-actin remodeling. PMID: 26413867
19. this study described for the first time the clinical, pathological and genetic features of DNM2-related CNM in Chinese patients. PMID: 25501959
20. Data indicate that dynamin 2 (Dyn2) interacts with Connexin 26 (Cx26) in a yeast two-hybrid screen, and co-localizes in mammalian cells. PMID: 25263585
21. Dynamin 2 is recruited as dimers during endocytic membrane scission at the neck of a clathrin-coated pit. PMID: 25232009
22. Thus dynamin that was recently found to control late stages of myoblast fusion also controls late stages of macrophage fusion, revealing an intriguing conserved mechanistic motif shared by diverse cell-cell fusion processes. PMID: 25336256
23. FGF21 has a role in promoting endothelial cell angiogenesis through a dynamin-2 and Rab5 dependent pathway PMID: 24848261
24. Inhibition of Dyn2 prevents cell invasiveness in androgen-responsive and -refractory PCA models, supporting the potential benefit of Dyn2 to serve as a therapeutic target for advanced prostate cancer . PMID: 24402972
25. These results reveal that girdin regulates selective clathrin-mediated endocytosis via a mechanism involving dynamin 2, but not by operating as a cargo-specific adaptor. PMID: 25061227
26. Upon overexpression of dynamin-2 mutants or depletion of clathrin heavy chain, flotillins are permanently trapped in endosomes. PMID: 24809731
27. dynamin2 recruitment is regulated by actin polymerization PMID: 24891602
28. Results indicate that hSNF5 affects both the stability and the activity of DNM2, uncovering an unexpected role of hSNF5 in modulating endocytosis, and open new perspectives in understanding the role of hSNF5 in tumour genesis. PMID: 23851497
29. results demonstrate a regulatory relationship between DNM2, miR-199a, and HIF, with implications in cancer metastasis PMID: 24706848
30. A mutation associated with centronuclear myopathy enhances the size and stability of dynamin 2 complexes. PMID: 24016602
31. These findings provide new evidence for the participation of the autolysosome in hepatocyte lipid droplet metabolism and demonstrate a novel role for dynamin2 in the function and maturation of an autophagic compartment. PMID: 24145164
32. Dual role of BAR domain-containing proteins in regulating vesicle release catalyzed by the GTPase, dynamin-2. PMID: 23861397
33. We identify dynamin and the EAP-binding alpha-adaptin appendage domain of the AP2 adaptor as switches in a regulated, multistep maturation process and provide direct evidence for a molecular checkpoint in clathrin mediated endocytosis. PMID: 23891661
34. The first homozygous mutation in the DNM2 protein p.Phe379Val, in three consanguineous patients with a lethal congenital syndrome, is reported. PMID: 23092955
35. Three novel DNM2 mutations in an Italy cohort of centronuclear myopathy patients. PMID: 23394783
36. This mutation (D614N) within the DNM2 gene in a large centronuclear myopathy family with a late age of overt clinical manifestation caused profound changes in DNM2 localization and impaired proper organization of myofibers and skeletal muscle function. PMID: 23374900
37. New onset strabismus and a history of a myopathy should consider this entity in the differential diagnosis that could be confirmed by a muscle biopsy and mutational analysis. PMID: 22924779
38. study provides evidence the large GTPase Dyn2 regulates the small GTPase Rac1 to potentiate invasive migration of pancreatic tumor cells; Dyn2 plays an essential role in regulating Rac1-mediated pancreatic tumor cell migration through modulation of the Rac1 activator Vav1 via a direct interaction PMID: 23537630
39. investigation of dynamin 2 expression in cervix of patients with suspected cervical intraepithelial neoplasia; evaluation of dynamin 2 expression as biological marker in diagnosis/staging/grading of cervical intraepithelial neoplasia PMID: 22959143
40. Dynamin II function is required for EGF-mediated Stat3 activation but not Erk1/2 phosphorylation PMID: 22574813
41. clinicopathological features of DNM2 centronuclear myopathy are rather homogeneous and can be distinguished from the features of non-DNM2 centronuclear myopathy PMID: 22613877
42. Caveolin-1 and dynamin-2 are essential for removal of the complement C5b-9 complex via endocytosis. PMID: 22528500
43. Defects in membrane trafficking due to DNM2 mutations potentially represent a common pathological mechanism in CNM and CMT. PMID: 22396310
44. DNM2 mutations cause autosomal dominant centronuclear myopathy or autosomal dominant (AD) Charcot-Marie-Tooth (CMT) disease; one large Czech family with 15 members affected with an AD CMT phenotype of extraordinary variability is reported. PMID: 22091729
45. this study identifies Dyn2 as an effector that mediates PDGFRalpha-SHP-2-induced glioma tumor growth and invasion PMID: 21996738
46. tumor cells overexpressing Dyn2 protruded lamellipodia at twice the rate, migrated faster (180%) and farther (2.5-fold greater distance) on glass and through transwell chambers PMID: 21841817
47. The alteration of microtubules by dynamin2 depletion reduced the length and the speed of the actin comet. PMID: 22174845
48. The mild functional defects are suggestive of differences between CMT and CNM disease-causing dynamin 2 mutants and suggest that a slight impairment in clathrin-mediated pathways may accumulate over time to foster the respective human diseases. PMID: 22096584
49. In summary, these results suggested that dynamin 2 might play a role in HIV-1 Env-mediated cell-cell fusion. PMID: 21338326
50. The deletion of PRD domain of dynamin 2 resulted in the impairment of both the localization and the abscission of daughter cells. PMID: 21150131
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相關疾病：

Myopathy, centronuclear, 1 (CNM1); Lethal congenital contracture syndrome 5 (LCCS5); Charcot-Marie-Tooth disease, dominant, intermediate type, B (CMTDIB); Charcot-Marie-Tooth disease 2M (CMT2M)
亞細胞定位：

Cytoplasm. Cytoplasm, cytoskeleton. Cell junction. Membrane, clathrin-coated pit. Cell junction, synapse, postsynaptic density. Cell junction, synapse. Midbody. Cell projection, phagocytic cup. Cytoplasmic vesicle, phagosome membrane; Peripheral membrane protein.
蛋白家族：

TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family
組織特異性：

Ubiquitously expressed.
數據庫鏈接：

HGNC: 2974

OMIM: 160150

KEGG: hsa:1785

STRING: 9606.ENSP00000347890

UniGene: Hs.211463

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