DDX5 Recombinant Monoclonal Antibody

說明書

中文名稱：

DDX5重組抗體
貨號：

CSB-RA231179A0HU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in: HepG2 whole cell lysate, HT-29 whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, Mouse brain tissue
All lanes: DDX5 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 70, 61 kDa
Observed band size: 70 kDa

IHC image of CSB-RA231179A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of HepG2 Cells with CSB-RA231179A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Overlay histogram showing Hela cells stained with CSB-RA231179A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1*10⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*10⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

DDX5重組單克隆抗體（產(chǎn)品編號：CSB-RA231179A0HU）是一款針對人源DDX5蛋白的高特異性檢測工具。DDX5作為DEAD-box RNA解旋酶家族成員，在RNA代謝、轉(zhuǎn)錄調(diào)控及細胞周期進程中發(fā)揮關(guān)鍵作用，其異常表達與腫瘤發(fā)生、轉(zhuǎn)移等病理過程密切相關(guān)。本抗體采用重組技術(shù)制備，經(jīng)多種實驗嚴格驗證，可應(yīng)用于ELISA、蛋白質(zhì)印跡（WB）、免疫組化（IHC）、免疫熒光（IF）及流式細胞術(shù)（FC）等檢測。在WB實驗中，抗體表現(xiàn)出高靈敏度，推薦使用1:500-1:5000稀釋比例；IHC和IF實驗推薦1:50-1:200稀釋，可清晰顯示組織或細胞內(nèi)的蛋白定位；FC分析建議1:20-1:200稀釋，適用于細胞表面或胞內(nèi)靶標(biāo)檢測。該抗體適用于探究DDX5在腫瘤微環(huán)境中的作用機制、RNA-蛋白互作研究、細胞增殖與分化模型構(gòu)建等科研領(lǐng)域，尤其在癌癥生物學(xué)、RNA代謝調(diào)控及表觀遺傳學(xué)研究中具有重要應(yīng)用價值。產(chǎn)品嚴格限于科學(xué)研究使用，實驗設(shè)計需結(jié)合具體研究需求優(yōu)化條件。
Uniprot No.：

P17844
基因名：

DDX5
別名：

Probable ATP-dependent RNA helicase DDX5 (EC 3.6.4.13) (DEAD box protein 5) (RNA helicase p68), DDX5, G17P1 HELR HLR1
反應(yīng)種屬：

Human, Mouse
免疫原：

A synthesized peptide derived from human DDX5
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

2C3
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200
FC	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.
基因功能參考文獻：
1. RIP-seq analysis in HEK293T cells identifies a complete repertoire of DDX5/p68 interacting transcripts including LOC284454 lncRNA. PMID: 29227193
2. Results showed that DDX5 was significantly up-regulated in gastric cancer tissues and revealed a novel role of DDX5 in gastric cancer cell proliferation via the mTOR pathway. PMID: 28216662
3. DDX5 is shown to be involved in RNA metabolism and viral infection, especially for RNA virus which seems to hijack host DDX5 to facilitate its own replication. However, DDX5 exhibits a role in antiviral responses during HBV and MYXV infection. Its opposite roles between DNA and RNA infection likely reflect the different modes of the biosynthesis of RNA and DNA viruses. [review] PMID: 29642538
4. A significant overlap between hnRNPA1 and DDX5 splicing targets and they share many closely linked binding sites. PMID: 30042133
5. The role of DDX5 in regulating esophageal cancer cell proliferation and tumorigenesis.DDX5 is highly expressed in esophageal cancer. PMID: 28244855
6. Downregulation of p68 RNA Helicase (DDX5) Activates a Survival Pathway Involving mTOR and MDM2 Signals.( PMID: 28557706
7. Results show refined biochemical and biological comparison of yeast Dbp2 and human DDX5 enzymes. Human DDX5 possesses a 10-fold higher unwinding activity than Dbp2, partially due to the presence of a mammalian/avian specific C-terminal extension. Also, ectopic expression of DDX5 rescues the cold sensitivity, cryptic initiation defects, and impaired glucose import in dbp2Delta cells, suggesting functional conservation. PMID: 28411202
8. p53 gain-of-function mutations accelerate endometrial carcinoma progression and metastasis by interfering with Drosha and p68 binding and pri-miR-26a-1 processing, resulting in reduced miR-26a expression and EZH2 overexpression. PMID: 26587974
9. Cervical cancer cell DDX5 gene is transcriptionally upregulated by calcitriol through a VDRE located in its proximal promoter. PMID: 26314252
10. Systematic Determination of Human Cyclin Dependent Kinase (CDK)-9 Interactome Identifies Novel Functions in RNA Splicing Mediated by the DEAD Box DDX5 and DDX17 RNA Helicases PMID: 26209609
11. LMTK3 escapes tumour suppressor miRNAs via sequestration of DDX5. PMID: 26739063
12. The data provide a model in which p68 and p53 interplay regulates PLK1 expression, and which describes the behavior of these molecules, and the outcome of their interaction, in human breast cancer. PMID: 24626184
13. Results show that a new mechanism of oncogenesis is attributed to p68 by upregulation of AKT and consequent nuclear exclusion and degradation of tumor suppressor FOXO3a. PMID: 25745998
14. DDX5 played an important role in the proliferation and tumorigenesis of non-small-cell lung cancer cells by activating the beta-catenin signaling pathway. PMID: 26212035
15. study shows that correction of p68 may reduce toxicity of the mutant RNAs in DM1 and in DM2 PMID: 26080402
16. Data indicate that cyclooxygenase 2 (COX-2) correlates inversely to microRNA 183 (miR-183) and directly to DEAD-box helicase p68 (DDX5). PMID: 25963660
17. DDX5 protein is essential for normal cell proliferation; (2) the transition from G1 to S/G2 phase is accompanied by an increase of DDX5 protein concentration in the cells. PMID: 26035968
18. AML is dependent on DDX5 and that inhibiting DDX5 expression slows AML cell proliferation. PMID: 24910429
19. Downregulation of DDX5 and DDX17 protein expression during myogenesis and epithelial-to-mesenchymal transdifferentiation contributes to the switching of splicing programs during these processes. PMID: 24910439
20. Data indicate that armadillo repeat protein ARVCF interacts with the splicing factors the splicing factor SRSF1 (SF2/ASF), the RNA helicase p68 (DDX5), and the heterogeneous nuclear ribonucleoprotein hnRNP H2. PMID: 24644279
21. In conclusion, authors identified DDX5 as a positive regulator for Japanese encephalitis virus replication via its binding to viral 3' UTR. PMID: 24035833
22. DDX5 facilitates HIV-1 replication as a cellular co-factor of Rev. PMID: 23741449
23. DDX5 might be critical for NOTCH1-mediated T-ALL pathogenesis and thus is a potential new target for modulating the Notch signaling in leukemia PMID: 23108395
24. p68, in the presence of Ca-calmodulin, can function as a microtubule motor. PMID: 23322042
25. Results show a novel role for DDX5 in cancer cell proliferation and suggest DDX5 as a therapeutic target in breast cancer treatment. PMID: 22750847
26. High p68 RNA helicase expression is associated with glioma. PMID: 22810421
27. Data indicate that transcriptional coregulator ddx5/ddx17 RNA helicases can simultaneously regulate the transcriptional activity and alternative splicing of NFAT5 transcription factor. PMID: 22266867
28. RNA helicases Ddx17 and Ddx5 contribute to tumor-cell invasiveness by regulating alternative splicing of several DNA- and chromatin-binding factors, including the macroH2A1 histone. PMID: 23022728
29. The DEAD box RNA helicase p68, also referred to as DDX5, directly interacts with VDR. PMID: 22476084
30. there is a direct interaction between DDX5 and NS5B and DDX5 has two independent NS5B-binding sites PMID: 22640416
31. High DDX5 is associated with basal breast cancer cells. PMID: 22086602
32. Using an RNA affinity pulldown-coupled mass spectrometry approach the study identified DDX5/RNA helicase p68 as an activator of TAU exon 10 splicing. PMID: 21343338
33. a striking inverse association of p68 and delta133p53 expression in primary breast cancers was identified. PMID: 20818423
34. DEAD-box RNA helicase p68 (DDX5) and its associated noncoding RNA, steroid receptor RNA activator (SRA), form a complex with CTCF that is essential for insulator function PMID: 20966046
35. Pleiotropic effects of p300-mediated acetylation on p68 and p72 RNA helicase. PMID: 20663877
36. crystallization and preliminary diffraction analysis of N-terminal region of DDX5 is reported.X-ray diffraction data were processed to a resolution of 2.7 A. PMID: 20124720
37. DDX5 is a repressor of fibrogenic genes in HSCs through interaction with transcriptional complexes. PMID: 20022962
38. Essential for pre-mrna splicing in vitro; may function in destabilizing the U1-5'ss interaction. Depletion of p68 RNA helicase arrested spliceosome assembly at the prespliceosome stage PMID: 12101238
39. synergism with transcriptional coactivators CBP and p300 PMID: 12527917
40. role in c-H-ras alternative splicing regulation PMID: 12665590
41. p68 is an important transcriptional regulator, functioning as a co-activator and/or co-repressor depending on the context of the promoter & the transcriptional complex. AA 1-478 of p68 can repress transcription as well as the full-length protein. PMID: 15298701
42. there is a tightly controlled expression and nucleolar localization of p68 in keratinocytes in vitro and during skin repair in vivo that functionally contributes to keratinocyte proliferation and gene expression PMID: 15304501
43. mechanism by which p68 may act as a tumour cosuppressor in governing p53 transcriptional activity PMID: 15660129
44. data suggest that function(s) of p68 RNA helicase may be subjected to the regulation of multiple cell signal pathways PMID: 15927448
45. In addition, it could be demonstrated that increasing the Tlk1 activity in HT1080 cells by forced Tlk1 overexpression leads to an increased phosphorylation of endogenous p68. PMID: 15950181
46. Patient with chronic hepatitis C carrying DDX5 haplotypes are at an increased risk of developing advanced liver fibrosis. PMID: 16697732
47. SUMO modification of the DEAD box protein p68 modulates its transcriptional activity and promotes its interaction with HDAC1 PMID: 17369852
48. A mutant that carries mutations at the phosphorylation sites (Y593/595F) dramatically sensitizes TRAIL-resistant cells to TRAIL-induced apoptosis, suggesting a potential therapeutic strategy to overcome TRAIL resistance. PMID: 17384675
49. The percentage correlation between Q-RT-PCR and microarray were 70% and 48% by using DDX5 and GAPDH as internal controls, respectively. PMID: 17540040
50. p68/p72 may contribute to colon cancer formation by directly up-regulating proto-oncogenes and indirectly by down-regulating the growth suppressor p21(WAF1/CIP1). PMID: 17699760
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亞細胞定位：

Nucleus. Nucleus, nucleolus. Cytoplasm.
蛋白家族：

DEAD box helicase family, DDX5/DBP2 subfamily
數(shù)據(jù)庫鏈接：

HGNC: 2746

OMIM: 180630

KEGG: hsa:1655

STRING: 9606.ENSP00000225792

UniGene: Hs.279806

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