CD86 Recombinant Monoclonal Antibody

中文名稱：

CD86重組抗體
貨號：

CSB-RA004965A0HU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in Hela whole cell lysate
All lanes CD86 antibody at 0.55μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 70 KDa
Observed band size: 70 KDa
其他：

產(chǎn)品詳情
產(chǎn)品評價
引用文獻(xiàn)
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

CD86 Recombinant Monoclonal Antibody（Code: CSB-RA004965A0HU）是針對免疫共刺激分子CD86開發(fā)的高特異性抗體。CD86作為B7家族重要成員，廣泛表達(dá)于抗原呈遞細(xì)胞表面，通過與T細(xì)胞表面CD28/CTLA-4受體相互作用，在免疫激活與耐受平衡中發(fā)揮核心調(diào)控功能。本抗體經(jīng)ELISA和Western Blot（WB）嚴(yán)格驗證，在WB實驗中推薦使用1:500-1:2000稀釋度，可清晰識別內(nèi)源性CD86蛋白條帶，驗證數(shù)據(jù)證實其優(yōu)異的靶標(biāo)結(jié)合能力和批次穩(wěn)定性，尤其適用于低豐度樣本檢測。該產(chǎn)品為研究免疫調(diào)控機制提供了可靠工具，可應(yīng)用于抗原呈遞細(xì)胞功能分析、T細(xì)胞活化研究、自身免疫性疾病模型及腫瘤免疫微環(huán)境等基礎(chǔ)科研領(lǐng)域，為探索免疫檢查點調(diào)控網(wǎng)絡(luò)及相關(guān)病理機制提供精準(zhǔn)的檢測支持。
Uniprot No.：

P42081
基因名：

CD86
別名：

CD86; CD28LG2; T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD antigen CD86
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

1G8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文獻(xiàn)

In situ injectable hydrogel-loaded drugs induce anti-tumor immune responses in melanoma immunochemotherapy J Li,Materials Today Bio,2023

產(chǎn)品評價

靶點詳情

功能：

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation.; Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.; (Microbial infection) Acts as a receptor for adenovirus subgroup B.
基因功能參考文獻(xiàn)：
1. Hepatitis C virus has a genetically determined lymphotropism through co-receptor B7.2. PMID: 28067225
2. this study shows that recipients' CD86 gene polymorphisms influence the overall survival after allogeneic hematopoietic stem cell transplantation and, together with CTLA-4 polymorphisms, might be considered a risk factor for acute graft versus host disease PMID: 29577049
3. results strongly suggest that CD40 and CD86 play a role in the pathophysiology of oral inflammatory diseases such as OLP PMID: 28904313
4. Our results reveal a role for B7-2 as obligatory receptor for superantigens. B7-2 homodimer interface mimotopes prevent superantigen lethality by blocking the superantigen-host costimulatory receptor interaction. PMID: 27708164
5. Our study reports a novel association of SNPs within CD86 and CTLA4 genes with pemphigus. The CD86 rs1129055 A allele appears to confer susceptibility to Pemphigus vulgaris but not to pemphigus foliaceus. PMID: 28274366
6. Our data demonstrate that CML patients with high CD86(+)pDC counts have a higher risk of relapse after TKI discontinuation. PMID: 28074067
7. IL-6, DEC205, and CD86 can be predictive biomarkers for the respiratory and immune effects of ambient PM2.5. PMID: 28056587
8. the upregulation of CD86 but not CD80 and PD-L1 on CD68+ cells in the liver of HBV-infected patients, observed in our study, suggest that the profile of CD68+ cells does not support the induction of proper Th1 responses that are needed to clear HBV infection. This might provide an explanation for the absence of potent HBV-specific T cells during chronic HBV infection. PMID: 27348308
9. CD86 variants association with susceptibility to multiple sclerosis in Iranian population. PMID: 28079472
10. B-cells from patients tolerant to the graft maintained higher IL-10 production after CD40 ligation, which correlates with lower CD86 expression compared to patients with chronic rejection. PMID: 26795594
11. TLR2, TLR4 and CD86 gene polymorphisms are associated with Recurrent aphthous stomatitis. PMID: 25482673
12. The SNP CD40 -1C>T was associated with the IgG response against PvDBP, whereas IgG antibody titers against PvMSP-119 were influenced by the polymorphism CD86 +1057G>A. PMID: 26901523
13. PD-L1 expression and the PD-L1/CD86 ratio in CD14(++)CD16(+) monocytes were higher during chronic hepatitis C virus infection. PMID: 24531620
14. Data show that induction of CD86 antigen expression on monocytes by human beta Defensin-3 (hBD-3) is suppressed by P2X7 purinoceptor (P2X7R) antagonist. PMID: 26416278
15. analysis of the expression of TLR-9, CD86, and CD95 in circulating B cells of patients with chronic viral hepatitis B or C before and after antiviral therapy PMID: 25892855
16. Polymorphisms in CD86 gene have diverse effects on the pathogenesis of pneumonia-induced sepsis. PMID: 25129060
17. CD86 +1057G/A polymorphism may be not associated with the genetic susceptibility to chronic immune thrombocytopenia in a Chinese population. PMID: 24897540
18. CD86 polymorphisms are associated with susceptibility to pneumonia-induced sepsis and may affect gene expression in monocytes. PMID: 25912130
19. CD86 polymorphisms (rs1129055) may have protective effects on cancer risk in Asians and that CD86 polymorphisms (rs17281995) is likely to contribute to risk of cancer, particularly colorectal cancer in Caucasians. PMID: 25369324
20. Results support a CTLA4-Ig/CD86 interaction on gammaIFN and IL-17 activated endothelial cells that modulates the expression of VEGFR-2 and ICAM1. PMID: 25896473
21. B7-2 costimulation and intracellular indoleamine 2,3-dioxygenase expression is reduced in umbilical cord blood as compared to adult peripheral blood. PMID: 24930629
22. Meningococcal capsular polysaccharide-loaded vaccine nanoparticles induce expression of CD86. PMID: 24981893
23. the higher levels of sCTLA-4 and CD86 in B-ALL patients might be candidate parameters for poor prognosis and may serve to refine treatment stratification with intensification of therapy in those patients prone to relapse. PMID: 24283754
24. no statistically significant difference between brucellosis patients and controls in the allele and genotype distributions of CTLA4, +49A/G (P = 0.859) and CD86, +2379G/C (P = 0.476) was found. PMID: 24298899
25. Cirrhotic patients with type 2 diabetes have increased expression of monocytic CD86 in comparison with cirrhotic non-diabetic, diabetic and healthy controls. This increases significantly with increase of the stage of the Child-Pugh score. PMID: 24378263
26. Provide evidence for an involvement of CD40+ and CD86+ B cells in the incidence of stroke and suggest that both pathogenic and protective B cell subsets exist. PMID: 24202305
27. Our results indicate that the methylation pattern in the CD86 promoter and CpG island is closely related to the expression of this co-stimulatory molecule in keratinocytes. PMID: 23867827
28. We failed to find any significant association of the 2 CD86 SNPs with RA. PMID: 23661460
29. Myeloid leukemia cells with a B7-2(+) subpopulation provoke Th-cell responses and become immuno-suppressive through the modulation of B7 ligands. PMID: 23175469
30. The frequency of the CD86 gene +1057A allele was significantly higher in pancreatic cancer cases than in controls. PMID: 22821131
31. CD86 and IL-12p70 are key players for T helper 1 polarization and natural killer cell activation by Toll-like receptor-induced dendritic cells. PMID: 22962607
32. Interaction of CD28 with B7 costimulatory antigen promotes proliferation and survival of activated gammadelta T cells following Plasmodium infection. PMID: 22732586
33. these results reveal the critical importance of the cytoskeleton-dependent CD86 polarization to the IS and more specifically the K4 motif for effective co-signaling. PMID: 22659416
34. CD86 represents an important tool for subdividing HSCs in several circumstances, identifying those unlikely to generate a full spectrum of hematopoietic cells. PMID: 22371880
35. Phe119 and Ser120 in the MIR2 ITM region and Asp244 in the B7-2 JM region contribute to the recognition of B7-2 by MIR2. PMID: 22379101
36. The +1057G/A polymorphism of the CD86 gene is associated with increased susceptibility to Ewing's sarcoma. PMID: 21870962
37. Data suggest that expression of CD86, CD80, and CD40 on dendritic cells in normal endometrium is higher than on tumor infiltrating dendritic cells in endometrioid adenocarcinoma; this may reflect roles in antigen presentation/tumor escape. PMID: 22142817
38. primary liver disease could influence the pre-transplantation levels of sCD86 and sCD95L. High pre-transplantation serum levels of sCD86 could favor the development of episodes of acute rejection. PMID: 22182632
39. IL-2 upregulates CD86 expression on human CD4(+) and CD8(+) T cells via a receptor-dependent mechanism that involves the NFAT and mammalian target of rapamycin pathways. PMID: 22246628
40. Yeast-derived beta-glucan lacks cytotoxic effects towards B-lymphoma cells but up-regulation of CD86 suggests maturation of the cells via dectin-1 by the carbohydrate PMID: 22199280
41. the +1057G/A polymorphism of the CD86 gene is associated with increased susceptibility to osteosarcoma PMID: 21563968
42. Parasite-induced B7-2 is dependent on Jun N-terminal protein kinase (JNK) but not extracellular signal-regulated kinase or p38 signaling; its expression on human peripheral blood monocytes is dependent on JNK signaling. PMID: 21911468
43. AA genotype and A allele of CD86 +1057G>A polymorphism may confer a protection against acute kidney allograft rejection in Tunisian patients. PMID: 21525579
44. Allergen exposure needs to cause weak or moderate cytotoxicity for DD86 and CD54 expression. PMID: 21628959
45. After surgery the rate of monocytes expressing B7-2 decreased in all the patients PMID: 21540807
46. genetic polymorphism is associated with risk or protection for chronic obstructive pulmonary disease in Chinese population PMID: 20732370
47. In the absence of irradiated M. tuberculosis, dendritic cells consist in a major DC-SIGN(high)/CD86(low) and minor DC-SIGN(low)/CD86(high) subpopulations, whereas in the presence of bacteria, there is an enrichment of DC-SIGN(low)/CD86(high) population. PMID: 20212510
48. In active ulcerative colitis CD86 and ICOS were over-expressed in the intestinal epithelial cells and lamina propria mononuclear cells. PMID: 20388394
49. Increased amount of CD86 or ICOS positive lamina propria mononuclear cells and enterocytes suggests that co-stimulatory molecules may play a role in the pathogenesis of Crohn disease. PMID: 20019769
50. Expansion of donor-derived lymphocytic choriomeningitis virus (LCMV)-specific CD4+ and CD8+ T cells is significantly impaired in B7.1/B7.2-deficient T cell receptor (TCR)transgenic recipients, compared with wild-type. PMID: 20601595
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亞細(xì)胞定位：

Cell membrane; Single-pass type I membrane protein.
組織特異性：

Expressed by activated B-lymphocytes and monocytes.
數(shù)據(jù)庫鏈接：

HGNC: 1705

OMIM: 601020

KEGG: hsa:942

STRING: 9606.ENSP00000332049

UniGene: Hs.171182

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