CD31 Recombinant Monoclonal Antibody

說明書

中文名稱：

CD31重組抗體
貨號：

CSB-RA017767A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in THP-1 whole cell lysate
All lanes CD31 antibody at 0.95μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 125 KDa
Observed band size: 125 KDa

IHC image of CSB-RA017767A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Overlay histogram showing Jurkat cells stained with CSB-RA017767A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CD31重組單克隆抗體（CSB-RA017767A0HU）是一種高特異性試劑，靶向細胞表面粘附分子CD31（也稱PECAM-1），該分子廣泛表達于血管內皮細胞、血小板及部分免疫細胞，在血管生成、白細胞遷移及炎癥反應中發揮關鍵調控作用。本抗體經多種技術平臺嚴格驗證：在Western Blot中可識別天然蛋白并呈現清晰單一信號條帶，推薦使用1:500-1:5000稀釋濃度；免疫組化實驗顯示其在石蠟包埋組織切片中對血管內皮標志物具有精準定位能力，建議工作濃度為1:50-1:500；流式細胞分析進一步證實其可有效標記活細胞表面CD31分子。該試劑適用于血管生物學研究，包括體外血管形成實驗、腫瘤血管異常性分析、心血管疾病模型中的內皮細胞動態追蹤，以及免疫微環境中內皮-免疫細胞相互作用研究等領域，為探索血管相關病理機制及藥物靶點篩選提供可靠工具。本產品僅限于科學研究使用。
Uniprot No.：

P16284
基因名：

PECAM1
別名：

PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31
反應種屬：

Human
免疫原：

A synthesized peptide
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

7B7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis.; Does not protect against apoptosis.
基因功能參考文獻：
1. High CD31 expression is associated with Central Giant Cell Granuloma. PMID: 30139237
2. TNF-alpha and IL-10 treatment can affect the expression of ICAM-1 and CD31 in human coronary artery endothelial cells. PMID: 29949812
3. High CD31 expression is associated with early-stage, but not in late-stage, laryngeal squamous cell carcinoma. PMID: 29523110
4. Adenocarcinomas showed significantly higher staining scores of both VEGF and alphaSMA than squamous cell carcinomas did. In 42 cases of high CD31 score, five-year survival rate (87%) of patients with lung cancer showing mature tumor vessels was significantly better than that (69%) of patients with immature tumor vessels PMID: 29970531
5. Differences in trafficking of CD31(+) cytotoxic T lymphocytes during acute influenza infection could modulate tolerance and contribute to a dampened adaptive immune response in neonates PMID: 28355204
6. Cell adhesion assays on wildtype and mutant PECAM-1 further characterized the structural determinants in cell junction and communication. PMID: 27958302
7. In primary hip OA, angiogenesis may be induced by a combined mechanism: hypoxia-related VEGF-dependent vasculogenesis and endothelial differentiation of the activated pluripotent cells, which are released from the hyperplastic synovial cells layer. An endothelial mesenchymal transition is assumed to be involved in the fibrotic process. PMID: 27704157
8. upregulation of sVEGFR-1 with concomitant decline of PECAM-1 and sVEGFR-2 levels in preeclampsia compared to normotensive pregnancies, Irrespective of the HIV status PMID: 28609170
9. Increased expression of PECAM-1, ICAM-3, and VCAM-1 in colonic biopsies from patients with inflammatory bowel disease (IBD) in clinical remission is associated with subsequent flares; this suggests that increases in the expression of these proteins may be early events that lead to flares in patients with IBD. PMID: 27552332
10. PECAM-1 gene polymorphisms are associated with Kawasaki disease with and without coronary artery lesions in Chinese children. PMID: 28512385
11. Sirt1 expression is associated with CD31 expression in endothelial progenitor cells from patients with chronic obstructive pulmonary disease. PMID: 27784320
12. we found a significant cumulative contribution of the genetic heterogeneity of glycoproteins Ia and IIIa and platelet-endothelial cell adhesion molecule-1 and P-Selectin genes in the risk of recurrent IVF-ET failures. The coexistence of these SNPs was associated with increased IVF-ET failure risk and the more polymorphic alleles or genotypes were present the higher the risk of IVF-ET failure, especially for younger women PMID: 28388872
13. Dimer conformation of soluble PECAM-1 PMID: 27270333
14. RrgA, binds both polymeric immunoglobulin receptor (pIgR) and PECAM-1, whereas the choline binding protein PspC binds, but to a lower extent, only pIgR PMID: 28515075
15. this study shows a significant role for CD31 during T cell development PMID: 28159903
16. Cells in high glucose for 7 days showed a significant decrease in mRNA expression of CD31 and VE-cadherin, and a significant increase in that of alpha-SMA and collagen I. PMID: 28347704
17. Patients who had optic neuritis that progressed to multiple sclerosis had a decrease in serum PECAM-1 levels. PMID: 27806869
18. Platelet endothelial cell adhesion molecule (PECAM-1) is expressed in endothelial cells (ECs), platelets, and leukocytes, regulating the interaction between those cells. PMID: 27079772
19. Data indicate no association of maternal or fetal ITGA2 C807T SNP, ITGB3 T1565C SNP, PECAM1 CTG - GTG and SELP A/C polymorphisms with fetal growth restriction (FGR). PMID: 28358707
20. these data suggest that a sialic acid-containing glycan emanating from Asn-25 reinforces dynamic endothelial cell-cell interactions by stabilizing the PECAM-1 homophilic binding interface. PMID: 27793989
21. Decreased FoxP3 expression in CD31(+) Tr cells. PMID: 27997991
22. These results suggested that PECAM-1 could mediate platelet adhesion to endothelial cells under shear stress. Platelets binding to endothelial cells interfered with endothelial cell mechanotransduction through PECAM-1, affecting endothelial cell inflammatory responses towards pathological shear flow. PMID: 28013181
23. Immunohistochemical expression of CD31 and vascular endothelial growth factor (VEGF) were assessed in parallel. PMID: 27270504
24. CD31 is expressed in mycosis fungoides (MF) skin biopsies, which provides new evidence for the role of angiogenesis in the progression of MF PMID: 27630298
25. The Leu125Val polymorphism of PECAM-1 and the level of soluble PECAM-1 are not associated with diabetic nephropathy in Caucasians with type 2 diabetes mellitus. PMID: 28116228
26. We provide the first report that pro-angiogenic genes PECAM1, PTGS1, FGD5, and MCAM may play a vital role in pathological dermal angiogenesis disorders of psoriasis. PMID: 26748901
27. The PECAM-1 functions as an adhesive stress-response protein to both maintain endothelial cell junctional integrity and speed restoration of the vascular permeability barrier following inflammatory or thrombotic challenge. PMID: 27055047
28. These studies indicate a role for PECAM-1 in enhancing the inhibitory functions of TGF-beta in T cells PMID: 26956486
29. PECAM1 plays an important role in the formation of tight junction complex. PMID: 26607202
30. The most significant associations were detected for PECAM1*V/V + DDAH1*C (OR = 4.17 CI 1.56-11.15 Pperm = 0.005) PMID: 26662939
31. soluble CD38 (sCD38) in seminal plasma increases the capacitation of sperm via specific interactions between sCD38 and the CD31 on the sperm. PMID: 26407101
32. expression levels of CD31/ PECAM1 are deregulated in human glioblastoma multiforme tissue specimens; correlation among CD31/PECAM1 and HIF-1alpha and N-cadherin and ADAM-10, two other markers of aggressiveness in the same tumors PMID: 26376118
33. Radiation-induced stress conditions induce a transient accumulation of granulocytes within the liver by down-regulation/absence of PECAM-1. PMID: 26177067
34. Suggest that CD31 expression correlates with prognosis in gastrointestinal stromal tumors. PMID: 26078569
35. PECAM-1 125C/G polymorphism is associated with deep vein thrombosis. PMID: 25846278
36. Heterogeneity was found in the endothelial cells: their shape, the expression of adhesion molecules(ICAM-1, VCAM-1, and PECAM ), and the adhesion of lymphocytes and monocytes to them changed during the progression of the atherosclerotic process. PMID: 26841644
37. PECAM1+ melanoma cells form vascular channels PMID: 25335460
38. Low shear stress can induce inflammatory response via PECAM-1/PARP-1/HMGB1 pathway. PMID: 25793984
39. Nck promoted oxidative stress-induced activation of NF-kappaB by coupling the tyrosine phosphorylation of PECAM-1 (platelet endothelial cell adhesion molecule-1) to the activation of p21-activated kinase PMID: 25714462
40. PECAM-1 has a role in mediating the profibrotic and prometastasic environment caused by ethanol in endothelial cells PMID: 24734240
41. Report correlation between mast cell tryptase and CD31 expression in odontogenic tumors. PMID: 26247531
42. there are associations between various PECAM-1 polymorphisms in rheumatoid arthritis and systemic lupus erythematosus patients, and PECAM-1 polymorphisms in SLE are protective against atherosclerotic complications PMID: 25201689
43. preeclampsia does not significantly affect vascular growth or the expression of endothelial junction proteins in human placentas PMID: 25362142
44. C-CD31 have impaired angiogenic potential and the number of circulating CD31(+) cells were correlated with cardiovascular disease risk PMID: 25267411
45. demonstrate a functional link between HO-1 gene expression and PECAM-1 in endothelial cells, which might play a critical role in the regulation of inflammation PMID: 24500083
46. The positive correlation is established between content of polymorphic nuclear monocytes and level of expression of molecules of LFA-1, ICAM-1, LFA-3, and PECAM-1. PMID: 25884075
47. High CD31 expression associated significantly with better survival and VEGFR3 had no association with survival. Both higher tumor grade and stage were associated with a decreased survival time PMID: 25667475
48. Suggest that cell confluence and the type of flow are critical independent factors in the induction of TF and PECAM-1 phosphorylation in endothelial cells exposed to disturbed pulsatile flow and chemical stimuli. PMID: 24342062
49. The finding that the adhesive properties of PECAM-1 are regulatable suggests novel approaches for controlling endothelial cell migration and barrier function in a variety of vascular permeability disorders. PMID: 24936065
50. for the cytological diagnosis of angiosarcomas, ERG and CD31 are more sensitive vascular markers than CD34 PMID: 25352641
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亞細胞定位：

Cell membrane; Single-pass type I membrane protein.; [Isoform Long]: Cell membrane; Single-pass type I membrane protein. Membrane raft. Cell junction.; [Isoform Delta15]: Cell junction. Note=Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
組織特異性：

Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level). Expressed on neutrophils (at protein level). Isoform Long predom
數據庫鏈接：

HGNC: 8823

OMIM: 173445

KEGG: hsa:5175

UniGene: Hs.376675

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