BIRC5 Recombinant Monoclonal Antibody

中文名稱：

BIRC5重組抗體
貨號：

CSB-RA209144A0HU
規(guī)格：

￥1320
圖片：
IHC image of CSB-RA209144A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
引用文獻(xiàn)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA209144A0HU BIRC5重組單克隆抗體是針對凋亡抑制蛋白Survivin開發(fā)的高特異性科研工具。該抗體靶向的BIRC5基因編碼蛋白在細(xì)胞周期調(diào)控和凋亡抑制中發(fā)揮重要作用，其異常高表達(dá)與多種腫瘤的增殖、轉(zhuǎn)移及治療抵抗密切相關(guān)。本產(chǎn)品采用重組表達(dá)技術(shù)制備，經(jīng)ELISA和免疫組化（IHC）嚴(yán)格驗(yàn)證，在IHC應(yīng)用中展現(xiàn)優(yōu)異的組織特異性識別能力，推薦使用稀釋比例為1:50-1:200時(shí)能清晰顯示目標(biāo)蛋白在細(xì)胞質(zhì)/核內(nèi)的定位分布，為腫瘤生物學(xué)研究提供可靠檢測支持。適用于石蠟包埋組織切片中BIRC5表達(dá)水平分析、癌癥發(fā)生發(fā)展機(jī)制研究，以及體外細(xì)胞模型中凋亡調(diào)控通路的探索，為探究腫瘤標(biāo)志物功能及藥物靶點(diǎn)篩選等基礎(chǔ)研究領(lǐng)域提供精準(zhǔn)的實(shí)驗(yàn)數(shù)據(jù)。
Uniprot No.：

O15392
基因名：

BIRC5
別名：

Baculoviral IAP repeat-containing protein 5 (Apoptosis inhibitor 4) (Apoptosis inhibitor survivin), BIRC5, API4 IAP4
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from human Survivin
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

6G11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文獻(xiàn)

LTBP4 Inhibits the Proliferation and Metastasis in Melanoma by Activating Hippo-YAP Signaling L na Wang,Research Square,2020

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Essential for the maintenance of mitochondrial integrity and function. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
基因功能參考文獻(xiàn)：
1. NF-kappaB activation may infer resistance to apoptosis through the expression of anti-apoptotic genes such as Survivin, which showed progressive increase in expression throughout the oesophageal metaplasia-dysplasia-adenocarcinoma sequence. PMID: 29473241
2. Study demonstrate that ZIC1 plays a tumor suppressive role in breast cancer, by targeting surviving, significantly downregulating its expression. PMID: 29956756
3. Studying survivin expression in leukocytes of 144 female rheumatoid arthritis patients this study observed that smoking patients had higher survivin transcription and a remarkable spreading of survivin isoforms. PMID: 27915033
4. Our findings identify survivin as a target of HO-1 and a mediator of adipocyte-induced survival in the metastatic niche. PMID: 29311669
5. High BIRC5 expression is associated with gefitinib resistance in lung cancer. PMID: 30106446
6. miR-203 expression also inhibited primary tumor growth in ovaries and metastatic tumors in multiple peritoneal organs including liver and spleen. miR-203 inhibits ovarian tumor metastasis by targeting BIRC5/survivin and attenuating the TGFbeta pathway. PMID: 30241553
7. Survivin may be implicated in the bcl-2 and p53 pathways and therefore in the biology of PDAC. Its potential use as a survival predictor and therapeutic target represent a promising field. PMID: 30249893
8. Study in hepatocellular carcinoma cell line elicited a new mechanism in which IGF-1 induced epithelial-mesenchymal transition through regulation of survivin and a downstream pathway. PMID: 29989646
9. In the present study, liposomeplasmid DNA encoding mutant survivinT34A could inhibit tumor growth of cervical cancer. This inhibition may be associated with an increase in the apoptosis rate of tumor cells and a reduction in angiogenesis. PMID: 29767242
10. simvastatin significantly inhibited the proliferation and invasion of SACC83 cells, induced apoptosis, and reduced the expression of survivin, which suggests that simvastatin may be a novel target for salivary gland adenoid cystic carcinoma therapy. PMID: 29956779
11. Survivin is significantly up-regulated in hepatocellular carcinoma (HCC)tissues and associated with tumor growth and lymph node metastasis. Clinical detection of survivin level combined with MRI examination might be beneficial for clinical diagnosis and treatment of HCC PMID: 30010107
12. Our study identified the STAT3 rs1053004 C/C as a high-risk genotype in MA with lower survivin and VEGF transcription levels in the peripheral blood. PMID: 30226700
13. Overexpression of miR-485-5p suppresses breast cancer progression and enhances chemosensitivity. Further study demonstrated that miR-485-5p directly targeted the 3'-untranslated region of survivin and overexpression of survivin overcomes the miR-485-5p induced effects on breast cancer. PMID: 29678577
14. Survivin expression in gastric cancer cells is regulated by DEC1. PMID: 29204860
15. High serum survivin levels with GG genotype are associated with Brain Tumors. PMID: 30275230
16. LNC473 could recruit deubiquitinase USP9X to inhibit the ubiquitination level of survivin and then increase survivin expression. PMID: 29605299
17. Oct4 plays a vital role in the malignant progression of HCC cells through the survivin/STAT3 signaling pathway. PMID: 29901157
18. Our study results may suggest that high serum survivin levels can show 4 times increased risk of cancer in a subject with a high suspicion of cancer. Furthermore, survivin level was not influenced with demographic characteristics of breast, gastric, colorectal, prostate, ovarian cancer, and glioblastome multiforme. PMID: 29893319
19. These findings collectively suggest that the triple combination of survivin knockdown with ABT-263 and trametinib treatment, may be a potential strategy for the treatment of KRAS-mutant lung adenocarcinoma. Furthermore, our findings indicate that the welldifferentiated type of KRAS-mutant lung tumors depends, at least in part, on TTF1 for growth. PMID: 29658609
20. Targeting the Cripto-1/TAK-1/NF-kappaB/Survivin pathway may be an effective approach to combat apoptosis resistance in cancer. PMID: 29807222
21. suggests SIRT1 may serve as a predictor of poor prognosis in esophageal squamous cell carcinoma, and its mediated tumor-promoting role might be associated with the overexpression of EGFR protein in esophageal squamous cell carcinoma PMID: 29625788
22. CSN5 directly bound survivin and decreased its ubiquitination to enhance the protein stability of survivin. PMID: 29596838
23. The survivin gene 3' UTR polymorphisms (rs17878624) show that GG genotype provides substantial protection from non-small-cell lung carcinoma. PMID: 29631694
24. Concomitant high expression of survivin and VEGF-C is closely associated with LNM status of PTC patients, which suggests their cooperation in the metastatic process. PMID: 29578160
25. In leukoplakia, the expression of survivin associated with that of ki-67 reinforces the assumption that all these lesions are potentially malignant. PMID: 28346726
26. Study showed for the first time that the suppression of rheumatiod arthritis fibroblast-like synoviocyte was mediated by phosphatase and tensin homolog involving survivin silencing. PMID: 28337018
27. ERCC1 expression may also inhibit esophageal squamous cell carcinoma cell apoptosis via regulating survivin expression, and ERCC1 and survivin overexpression are independent predictors of prognosis for ESCC patients who receive chemotherapy and/or radiotherapy PMID: 30075571
28. let-7b targets PLK1 to inhibit hepatocellular carcinoma cell growth and induce their apoptosis by attenuating the PLK1-mediated Survivin phosphorylation PMID: 29913237
29. Overexpression of Survivin in glioma cells induces chromosomal instability PMID: 29282022
30. High BIRC5 expression is associated with ovarian cancer. PMID: 29795564
31. In III-rd trimester of pregnancy parameters of Timp-1 and Survivin - anti-apoptotic substances concentration were similar in maternal and cord blood in both artery and vein. We found no increased activity of selected antiapoptotic factors. PMID: 28509321
32. Results form study in non-small-cell lung cancer cells showed that SphK2 plays a critical role in doxorubicin-induced resistance by regulating key anti-apoptotic gene, survivin. PMID: 28950390
33. Survivin overexpression plays a key role in the chemoresistance of ovarian CSCs. PMID: 30061219
34. Survivin may play an important role in the occurrence and development of laryngeal carcinoma, and its high expression is related to the poor prognosis of patients with laryngeal cancer. (Meta-analysis) PMID: 29270761
35. treatment of DLD1 cells with tamoxifen , betaestradiol, or a combination of these two drugs, inhibited cell viability and migration, promoted cell apoptosis, and reduced the mRNA and protein expression levels of survivin in a dose and timedependent manner. These results provide novel experimental basis for hormonal adjuvant therapy for the treatment of colorectal cancers PMID: 28849238
36. Studies indicated that high survivin expression in renal cell carcinoma (RCC) was associated with poor overall survival [Review]. PMID: 27411378
37. Nuclear accumulation of survivin is associated with proliferative phenotype and was shown to be a worse prognostic marker in breast ductal carcinoma. PMID: 29517199
38. Knockdown of BIRC5, a member of the inhibitor of apoptosis protein family, using either lentiviral vector based CRISPR/Cas9 nickase gene editing or inhibition of survivin using the small-molecule inhibitor YM155, results in the suppression of epithelial to mesenchymal transition in retinal pigment epithelial cells. PMID: 29522718
39. By downregulation of Sp1 and survivin at the late phase of treatment. PMID: 28713892
40. The expressions of survivin and STAT2 are up-regulated in skin lesions of PV patients, and their mRNA expressions are positively correlated. PMID: 29089085
41. Findings suggest that lysosome-associated transmembrane protein 4B (LAPTM4B), vascular endothelial growth factor (VEGF), and nuclear survivin expression are significantly correlated in breast cancer, which may be predictive of prognosis as well as effective therapeutic targets for anticancer therapies. PMID: 28476037
42. HIF-2alpha dictates the resistance of human pancreatic cancer cells to TRAIL under normoxic and hypoxic conditions and transcriptionally regulates survivin expression. PMID: 28476028
43. survivin may have a role in recurrence in rectal cancer patients treated with surgery and postoperative concurrent chemo-radiation therapy PMID: 27391438
44. Results identified BIRC5 to be significantly upregulated in the lung squamous cell carcinoma tissues of smoking patients and may play an important role in diagnosis and prognosis. PMID: 28949095
45. After cancer cell fusion, some fused cells avoid the apoptotic crisis partly owing to survivin, and continue to proliferate, a process that contributes to human cancer progression. PMID: 28193315
46. High survivin expression is associated with lung cancer and colorectal cancer. PMID: 27602754
47. Data suggest that Ki-67 index and survivin may be useful biomarkers for rectal cancer with preoperative chemoradiotherapy. PMID: 29491110
48. inhibition of apoptosis targeting survivin might represent an effective strategy for both obesity and cancer therapy. PMID: 28518147
49. FAT10 promotes tumor proliferation by directly stabilizing Survivin protein in breast cancer cells. PMID: 27806337
50. nuclear survivin is a prognostic marker for the progression of oral squamous cell carcinomas. PMID: 28384094
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亞細(xì)胞定位：

Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody.
蛋白家族：

IAP family
組織特異性：

Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines. Ex
數(shù)據(jù)庫鏈接：

HGNC: 593

OMIM: 603352

KEGG: hsa:332

UniGene: Hs.744872

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