AURKB Recombinant Monoclonal Antibody

說明書

中文名稱：

AURKB重組抗體
貨號：

CSB-RA230110A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate
All lanes: AURKB antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40, 36, 17, 35 kDa
Observed band size: 40 kDa

IHC image of CSB-RA230110A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA230110A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunoprecipitating AURKB in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA230110A0HU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA230110A0HU(2μg)+ Hela whole cell lysate(500μg)
Lane 3: Hela whole cell lysate (10μg)
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA230110A0HU AURKB重組單克隆抗體是針對極光激酶B（Aurora Kinase B）開發的高特異性科研試劑。該抗體采用重組技術制備，具有嚴格的批次間一致性，適用于多種實驗場景。經嚴格驗證，其在Western Blot中能清晰識別天然及過表達的AURKB蛋白，推薦使用稀釋度為1:500-1:5000；免疫組化實驗顯示優異的組織定位特異性，建議稀釋比例1:50-1:200；同時支持免疫沉淀（IP）分析，推薦使用濃度1:200-1:1000。AURKB作為有絲分裂核心調控因子，在染色體分離和胞質分裂中發揮關鍵作用，其異常表達與腫瘤發生發展密切相關。本產品特別適用于細胞周期研究、腫瘤相關信號通路探索以及藥物靶點篩選等科研領域，為體外培養細胞的蛋白互作研究、腫瘤組織樣本分析及分子機制解析提供可靠工具。實驗數據表明該抗體在不同物種來源的樣本中均表現出良好的交叉反應性，其低背景特性可顯著提升實驗結果的可信度。
Uniprot No.：

Q96GD4
基因名：

AURKB
別名：

Aurora kinase B (EC 2.7.11.1) (Aurora 1) (Aurora- and IPL1-like midbody-associated protein 1) (AIM-1) (Aurora/IPL1-related kinase 2) (ARK-2) (Aurora-related kinase 2) (STK-1) (Serine/threonine-protein kinase 12) (Serine/threonine-protein kinase 5) (Serine/threonine-protein kinase aurora-B), AURKB, AIK2 AIM1 AIRK2 ARK2 STK1 STK12 STK5
反應種屬：

Human
免疫原：

A synthesized peptide derived from human Aurora B
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

4E5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IP	1:200-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPTIN1, VIM/vimentin, HASPIN, and histone H3. A positive feedback loop involving HASPIN and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between HASPIN and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGO1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes.
基因功能參考文獻：
1. We also suggested Aurora-B as a promising therapeutic target in non-small cell lung cancer treatment. PMID: 29707994
2. The epigenetic targets AURKB, AURKC and DNMT3B, and the global DNA methylation profile are regulated during HIV-1 replication in CD4+ T cells, and this regulation can be influenced by the activation state of the cell at the time of infection. PMID: 30077875
3. findings suggest that USP35 regulates the stability and function of Aurora B by blocking APC(CDH1)-induced proteasomal degradation, thereby controlling mitotic progression PMID: 29449677
4. PKCvarepsilon directly modulates the Aurora B-dependent abscission checkpoint by phosphorylating Aurora B at S227. This phosphorylation invokes a switch in Aurora B specificity, with increased phosphorylation of a subset of target substrates, including the CPC subunit Borealin. PMID: 28004745
5. The results propose a model in which Aurora B-mediated H2AX-phosphorylated serine 121 probably provide a platform for Aurora B autoactivation circuitry at centromeres and thus play a pivotal role in proper chromosome segregation. PMID: 27389782
6. The data suggest that AKA is the vertebrate ancestral gene, and that AKB and AKC resulted from gene duplication in placental mammals. PMID: 29283376
7. Study reveals the mechanism controlling abscission through integration of Aurora B kinase and B56-bound PP2A phosphatase activities on the kinesin motor protein MKlp2. MKlp2 is an essential protein for promoting abscission, which may regulate tethering and stabilizing of the PM to the microtubule cytoskeleton at the intercellular bridge through its previously uncharacterized lipid association motif. PMID: 27939310
8. we identified deguelin as an effective Aurora B inhibitor, which deserves further studies in other animal models and esophageal squamous cell carcinoma treatment PMID: 29129699
9. Our data show that S49076 exerts its cytotoxic activity at low doses on MET-dependent cells through MET inhibition, whereas it inhibits growth of MET-independent cells at higher but clinically relevant doses by targeting Aurora B PMID: 28619752
10. Results show the overexpression Aurkb decreased glycolytic activities and suggest that AURKB is involved in asthenozoospermia. PMID: 29653228
11. Identification and characterization of AURKB and AURKC variants associated with maternal aneuploidy has been reported. PMID: 28369513
12. SIX3 is a novel negative transcriptional regulator and acts as a tumor suppressor that directly represses the transcription of AURKA and AURKB in astrocytoma. PMID: 28595628
13. Our findings suggested that AURKA (rs911160) and AURKB (rs2289590) polymorphisms could affect GC risk. Further validation studies in larger and multi-ethnical populations are needed to elucidate their functional impact on the development of GC PMID: 28843004
14. Data show that aurora-B regulates end-on conversion in human cells and indicate a late role for SPAG5 protein (Astrin)-SKAP complex in the end-on conversion process. PMID: 28751710
15. The results of experiment indicated that specific knockdown of Aurora kinase B led to prostate carcinoma cells apoptosis and inhibited tumor growth. PMID: 28100163
16. decrease in Aurora B results in diminished binding of the chromokinesin Kif4A to chromosome arms. PMID: 28821562
17. Aurora B kinase interacts with and phosphorylates Sgo1. Aurora B-mediated phosphorylation of Sgo1 regulates the distribution of Sgo1 between centromeres and chromosome arms. PMID: 25451264
18. AURKC rs758099 TT and (CC + CT) genotypes were positively associated with increased intestinal type gastric cancer (GC)risk, but not with an increased diffuse type GC risk. Based on these results, we can conclude that AURKA rs1047972 and AURKC rs758099 polymorphisms could affect the risk of GC development. PMID: 27270838
19. Aurora-C interactions with members of the Chromosome Passenger Complex (CPC), Survivin and Inner Centromere Protein (INCENP) in reference to known Aurora-B interactions to understand the functional significance of Aurora-C overexpression in human cancer cells, is reported. PMID: 27332895
20. Aurora kinase inhibitor danusertib negatively regulated AURKB/p70S6K/RPL15 axis with the involvement of PI3K/Akt/mTOR, AMPK, and p38 MAPK signaling pathways, leading to the induction of apoptosis and autophagy in human leukemia cells. PMID: 27612557
21. The kinase activity of Aurora B on serine 31 of histone H3.3 was biochemically confirmed with nucleosomal substrates in vitro. PMID: 28137420
22. in addition to its role in checkpoint signaling, MAD2 ensures chromosome stability through the regulation of AURORA B. PMID: 27341405
23. Ska promotes Aurora B activity to limit its own microtubule and kinetochore association. PMID: 27697923
24. partial co-localization of AKT3 with AURKB was observed during anaphase. Overall, this study suggests that AKT3 could repress the antiproliferative effects of AURKi, with a novel activity particularly suppressing the aneuploidy induction. PMID: 28028179
25. Our study mechanistically explains a p53-independent mode of action of a chemical Aurora B inhibitor and suggests a potential triggering of antitumoral immune responses, following polyploidization of tumor cells, which might constrain recovery of aneuploid tumor cells. PMID: 27515963
26. Our data demonstrate that the cytokinetic proteins epithelial cell transforming 2 and Aurora kinase B (AurkB) are localized to stress granules in human astrocytoma cells PMID: 27106762
27. a resorcinol derivative, 5-methyl-4-(2-thiazolylazo) resorcinol (PTK66), a dual inhibitor of Aurora A and Aurora B kinases. PTK66 is a surface binding non-ATP analogue inhibitor that shows a mixed pattern of inhibition against both of Aurora A and B kinases PMID: 26808391
28. overexpression of PAK1, NEK6, AURKA, and AURKB genes in patients with Colorectal adenomatous polyp and colorectal cancer in the Turkish population. PMID: 26423403
29. Aurora-B and HDAC can cooperatively regulate AKT, mTOR and Notch pathways PMID: 26638998
30. Proper midbody architecture requires cross-regulation between two cell division kinases, Citron kinase (CIT-K) and Aurora B, the kinase component of the chromosomal passenger complex. PMID: 27009191
31. Aurora B may prefer Cdk1-phosphorylated Sororin as a substrate. PMID: 26177583
32. Aurora kinases are important KRAS targets in lung cancer. PMID: 26842935
33. Aurora A and B inhibition had a significant antitumor effect against TNBC, and this antitumor effect was maximized by the combination of selumetinib with Aurora A and B inhibition. PMID: 26443806
34. Clk1, Clk2 and Clk4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint. PMID: 27126587
35. Combined treatment with aurora kinase inhibitor SNS-314and quercetin-hyaluronic acid hydrogel results in synergistic cytotoxic effects in papillary and medullary thyroid carcinoma cells. PMID: 26660542
36. NudC is co-localized with Aurora B at the midbody and co-immunoprecipitated with Aurora B in mitosis. results suggest that that dynamic phosphorylation of NudC by Aurora B regulates cytokinesis. PMID: 27074040
37. Aurora B and CDK1 temporally regulate the binding affinity of EB2 for microtubules, thereby ensuring kinetochore microtubule dynamics, proper mitotic progression and genome stability. PMID: 27030108
38. the Aurora B-PLK1 signaling at the kinetochore orchestrates MCAK activity, which is essential for timely correction of aberrant kinetochore attachment to ensure accurate chromosome segregation during mitosis. PMID: 26206521
39. PC4 associates with Aurora A and Aurora B and undergoes phosphorylation, following which PC4 activates both Aurora A and B to sustain optimal kinase activity to maintain the phosphorylation gradient for the proper functioning of the mitotic machinery. PMID: 26777301
40. HP1 binding to the chromosomal passenger complex becomes particularly important when Aurora B phosphorylates kinetochore targets to eliminate erroneous microtubule attachments PMID: 26954544
41. UBASH3B is necessary for the timing and fidelity of chromosome segregation in human cells PMID: 26766443
42. These results demonstrate that Aurora B inhibits both direct interaction with the microtubule and oligomerization of the Dam1 complex to drive error correction during mitosis. PMID: 26560693
43. Aurora B plays role in maintaining genomic integrity by promoting the formation of a passageway in the nuclear envelope through which late-segregating acentric chromosomes enter the telophase daughter nucleus. PMID: 25877868
44. Data indicate the antitumor and radiosensitizing activity of daurinol in lung cancer cells through the inhibition of aurora kinase A/B (AURKA and AURKB). PMID: 25882311
45. Study reports that the monomeric RING domains from the human E3 ligases Arkadia and Ark2C bind directly to free ubiquitin with an affinity comparable to that of other dedicated ubiquitin-binding domains. PMID: 26656854
46. Enhanced let-7i expression suppresses cell migration and invasion of osteosarcoma (OS) cells in vitro. Let-7i inhibits OS cell malignant phenotype partly by targeting Aurora-B. PMID: 25997616
47. Disrupting AurKB before mitotic entry reduces chromosomal passenger complex (CPC) localization to the centromere. AurKB plays a minimal role in maintaining CPC localization once cells are in mitosis. PMID: 25854549
48. Results suggest the potential for therapeutic application of Aurora kinase B inhibitors in the treatment of MYC overexpressing medulloblastoma. PMID: 25739120
49. Aurora kinases ((Aurora kinase A and B )mediate PKC-MAPK signal to NF-kappaB/AP-1 with increasing MMP-9 expression and invasion of MCF-7 cells. PMID: 26044736
50. midostaurin treatment significantly reduced the Aurora kinase-mediated phosphorylation reactions in the cell line. Although the effect was weaker than that of VX-680, midostaurin attenuated the phosphorylation of Aurora kinases A and B PMID: 26141684
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相關疾病：

Disruptive regulation of expression is a possible mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
亞細胞定位：

Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Midbody.
蛋白家族：

Protein kinase superfamily, Ser/Thr protein kinase family, Aurora subfamily
組織特異性：

High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
數據庫鏈接：

HGNC: 11390

OMIM: 604970

KEGG: hsa:9212

STRING: 9606.ENSP00000463999

UniGene: Hs.442658

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