ATP1A1 Recombinant Monoclonal Antibody

說明書

中文名稱：

ATP1A1重組抗體
貨號：

CSB-RA796310A0HU
規(guī)格：

￥1320
圖片：
IHC image of CSB-RA796310A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA796310A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

ATP1A1重組單克隆抗體（CSB-RA796310A0HU）是針對鈉鉀ATP酶α1亞基（ATP1A1）的高特異性科研工具，該靶蛋白作為細胞膜離子轉(zhuǎn)運的核心組分，通過水解ATP驅(qū)動鈉鉀離子跨膜運輸，在維持細胞滲透壓平衡、神經(jīng)沖動傳導及心肌收縮等基礎生理過程中發(fā)揮關鍵作用。本抗體采用重組技術制備，具有優(yōu)異的批間一致性，經(jīng)過嚴格驗證，適用于ELISA及免疫組化（IHC）實驗，其中IHC推薦使用1:50-1:200稀釋比例，可在石蠟包埋組織中清晰呈現(xiàn)ATP1A1的亞細胞定位及表達水平。驗證數(shù)據(jù)表明其與人類ATP1A1蛋白特異性結合，且未檢測到與其他鈉鉀ATP酶亞型（如ATP1A2/A3）的交叉反應。該產(chǎn)品適用于探究ATP1A1在細胞離子穩(wěn)態(tài)調(diào)控、能量代謝相關通路中的功能機制，或用于心血管疾病、神經(jīng)退行性疾病等科研模型中靶蛋白表達異常的檢測分析，為細胞生物學、病理生理學及藥物靶點篩選等基礎研究提供可靠工具。
Uniprot No.：

P05023
基因名：

ATP1A1
別名：

Sodium/potassium-transporting ATPase subunit alpha-1 (Na(+)/K(+) ATPase alpha-1 subunit) (EC 3.6.3.9) (Sodium pump subunit alpha-1), ATP1A1
反應種屬：

Human
免疫原：

A synthesized peptide derived from human Sodium Potassium ATPase
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

4C3
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
基因功能參考文獻：
1. mutations in ATP1A1, which encodes the alpha1 subunit of the Na(+),K(+)-ATPase, are identified as a cause of autosomal-dominant CMT2. PMID: 29499166
2. Low ATP1A1 expression is associated with renal cell carcinoma. PMID: 30015972
3. These data suggest the role of beta-amyloid as a novel physiological regulator of Na,K-ATPase. PMID: 27296892
4. induction of a novel pathway (alpha1-AMPK-ULK1) induces autophagy as a host-directed strategy for HCMV inhibition.IMPORTANCE PMID: 29321306
5. An antibody against the extracellular DR region (897-911) of Na(+)-K(+)-ATPase subunit alpha 1 disrupted the Na+-K+-ATPase/ROS amplifier and protected cultured cardiomyocytes from ROS-induced injury. PMID: 28181111
6. Disruption of Ankyrin B and Caveolin-1 Interaction Sites Alters Na(+),K(+)-ATPase Membrane Diffusion PMID: 28988699
7. Mutations in ATP1A1 cause the excessive autonomous aldosterone secretion of Aldosterone-producing Adenomas. PMID: 28584016
8. Cardiotonic steroids activate NF-kappaB leading to proinflammatory cytokine production in primary macrophages through a signaling complex, including CD36, TLR4, and Na/K-ATPase. PMID: 28619997
9. This study reports molecular dynamic simulations of the human NaK-ATPase alpha1 beta 1 isoform embedded into 1,2-oleoylphosphatidylcholine bilayer. PMID: 27966362
10. Data show that the expression of aquaporin (AQP) 1, AQP3, AQP5, epithelial Na+ channel (ENaC) and sodium potassium ATPase (Na-K-ATPase) are altered in patients with acute respiratory failure (ARF) due to diffuse alveolar damage (DAD), and the cause of DAD does not seem to influence the level of impairment of these channels. PMID: 27835672
11. Ouabain stimulates NKA in renal proximal tubule cells through an angiotensin/AT1R-dependent mechanism and that this pathway contributes to cardiac glycoside associated hypertension. PMID: 27496272
12. Reduction in ATP1A1 expression levels is related to major depressive disorder anxiety score. PMID: 27487491
13. Our current findings demonstrate that Arctigenin is an antagonist of MR and effectively decreases the Na/K-ATPase 1 gene expression. Our work provides a hint for the drug discovery against cardiovascular disease PMID: 26446917
14. Data suggest that targeting Na(+)/K(+) ATPase alpha-1 subunit (ATP1A1) is a approach to the treatment of hepatocellular carcinoma (HCC). PMID: 26334094
15. Mutations in ATP1A1 gene is associated with aldosterone-producing adenomas. PMID: 26285814
16. Gal-3 interacts with ATP1A1 and induces the phosphorylation of MDR1, mediating multidrug resistance. PMID: 26158764
17. protein kinase A and C activation can increase Na,K-ATPase activity in human muscle but not via P2Y receptor stimulation. PMID: 24614174
18. Mutations of the ATP1A1 depolarize adrenocortical cells, disturb the K+ sensitivity, and lower intracellular pH but do not induce an overt increase of intracellular Ca2+. PMID: 26418325
19. Different mutations (KCNJ5, ATP1A1, ATP2B3, and CACNA1D) are found in different aldosterone-producing nodules from the same adrenal, suggesting that somatic mutations are independent events triggered by mechanisms that remain to be identified. PMID: 26351028
20. Data shows that a functional 12T-insertion polymorphism in the ATP1A1 promoter confers decreased susceptibility to hypertension in a male Sardinian population supporting ATP1A1 as a hypertension susceptibility gene in this population. PMID: 25615575
21. Data show that the intracellular M4M5 loop of human alpha1 subunit of Na+,K+-ATPase (ATP1a1) interacts directly with rat rab GTP-binding protein Rab27a. PMID: 25051489
22. Ebola VP24 protein plays a critical role in the evasion of the host immune response by interacting with human ATP1a1 protein. PMID: 25158218
23. Studies indicate specific lipid-binding sites in Na,K-ATPase E1 and E2 crystal structures. PMID: 25791351
24. Report tumor cell sensitivity to cardiac glycosides depends on pattern of expression of alpha1-, alpha2-, or alpha3-isoforms of Na-K-ATPase. PMID: 25994790
25. ATP1A1-mediated Src signaling inhibits coronavirus entry into host cells. PMID: 25653449
26. The cytoplasmic domain of ATP1A1 directly interacts with FGF2 and is required for FGF2 secretion. PMID: 25533462
27. While JNK exerts an inhibitory effect on the ATPase, NF-kappaB increases its activity and abrogates the stimulatory effect of the sphingolipid on JNK leading thus to an additional increase in the ATPase activity. PMID: 24819540
28. Somatic mutations found in KCNJ5, ATP1A1, and ATP2B3 appear to be the driving forces for a higher aldosterone production and proliferations of glomerulosa cells. PMID: 24179102
29. H-K-ATPase alpha-subunit mRNA 3' UTR to binding of miR-1289 identifies a novel regulatory mechanism of gastric acid secretion and offers new insights into mechanisms underlying transient H. pylori-induced hypochlorhydria. PMID: 24503769
30. JAK2 is a novel energy-sensing kinase that curtails energy consumption by downregulating Na(+)/K(+)-ATPase expression and activity. PMID: 24304834
31. Data indicate that overexpression of ouabain-insensitive rat Na(+)/K(+)-ATPase alpha1 failed to inhibit internalization of human Na(+)/K(+)-ATPase alpha1 expressed in the same cells. PMID: 24275648
32. ATP1A1 mutations are present in aldosterone-producing adenomas that result in an increase in CYP11B2 gene expression and may account for the dysregulated aldosterone production in a subset of patients with sporadic primary aldosteronism. PMID: 24082052
33. Somatic mutations in either ATP1A1 and CACNA1D were found in a subset of adrenal aldosterone-producing adenomas with a zona glomerulosa-like phenotype. PMID: 23913004
34. Evidence for a new mechanism by which hypercapnia via soluble adenylyl cyclase, cAMP, PKA Type Ialpha, and alpha-adducin regulates Na,K-ATPase endocytosis in alveolar epithelial cells. PMID: 23349050
35. Somatic mutations in ATP1A1 gene leads to aldosterone-producing adenomas and secondary hypertension. PMID: 23416519
36. ZNRF1 and ZNRF2 are new players in regulation of the ubiquitous Na(+)/K(+)ATPase that is tuned to changing demands in many physiological contexts. PMID: 22797923
37. NRF-1 regulates Atp1a1 and Atp1b1 and are important in mediating the energy generation and neuronal activity. PMID: 23048038
38. Ouabain could up-regulate Na+, K(+)-ATPase alpha1 subunit expression and reduce beta1-subunit expression which mediated signal transduction and decreased cell-cell adhesions and induced ECV304 cell death. PMID: 21141520
39. Data show that Na(+),K(+)-ATPase activity was >50% lower and membrane-associated tubulin content was >200% higher in erythrocyte membranes from diabetic patients. PMID: 22565168
40. A significant increase in the expression of ATP1A1, which encodes the alpha1-subunit of the Na(+)/K(+)-ATPase, was identified in HNF1B patients consistent with its role in Mg(2+) homeostasis. PMID: 22269832
41. the sodium pump alpha1 sub-unit has a role in progression of metastatic melanoma PMID: 19243476
42. FXYD1 raises the affinity of the human alpha1beta1 isoform of Na,K-ATPase for Na ions PMID: 21449573
43. There was overexpression of the alpha1 or alpha3 NaK subunits in more than half of the medulloblastomas. PMID: 21498719
44. The increased maternal AChE and Na(+), K(+)-ATPase activities may be due to the low total antioxidant status determined post vaginal delivery, whereas their decreased activities in prematures to their immaturity. PMID: 20964587
45. Studies indicate that change of Na,K-ATPase activity can influence neurotransmitter release. PMID: 21043236
46. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20628086
47. Silencing ATP1A1 inhibits the proliferation of HepG2 cells by decreasing the expression of MAPK1 and induces cell cycle arrest. PMID: 20450619
48. Results suggest that the association of NHE-1 with Na-K-ATPase is critical for ouabain-mediated regulation of Na-K-ATPase and that these effects may play a role in cardioglycoside-stimulated hypertension. PMID: 20427472
49. These results suggest that Akt plays a major role in Na(+)/K(+)-ATPase intracellular translocation and thus in alveolar fluid reabsorption. PMID: 20332111
50. Data conclude that human proximal tubular cells respond to a hyperosmotic challenge with an increase in FXYD2 and Na,K-ATPase protein expression, though to a smaller absolute extent in patient cells. PMID: 19865785
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亞細胞定位：

Basolateral cell membrane; Multi-pass membrane protein. Cell membrane, sarcolemma; Multi-pass membrane protein. Cell projection, axon. Melanosome.
蛋白家族：

Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIC subfamily
數(shù)據(jù)庫鏈接：

HGNC: 799

OMIM: 182310

KEGG: hsa:476

STRING: 9606.ENSP00000295598

UniGene: Hs.371889

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