ATM Recombinant Monoclonal Antibody

說明書

中文名稱：

ATM重組抗體
貨號：

CSB-RA166706A0HU
規(guī)格：

￥1320
圖片：
IHC image of CSB-RA166706A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA166706A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Overlay histogram showing Hela cells stained with CSB-RA166706A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1*10⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*10⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA166706A0HU ATM重組單克隆抗體是針對ATM蛋白研發(fā)的高特異性檢測工具。ATM（共濟(jì)失調(diào)毛細(xì)血管擴張癥突變蛋白）是PI3K相關(guān)激酶家族成員，在DNA雙鏈斷裂修復(fù)中發(fā)揮核心作用，通過激活p53、CHK2等下游靶點調(diào)控細(xì)胞周期檢查點并維持基因組穩(wěn)定性。本產(chǎn)品經(jīng)多種技術(shù)平臺驗證，在免疫組化（IHC）1:50-1:200稀釋范圍內(nèi)可清晰顯示ATM在細(xì)胞核內(nèi)的定位特征，流式細(xì)胞術(shù)（FC）在1:20-1:200濃度梯度下能精確檢測細(xì)胞群體中ATM的表達(dá)水平，配套的ELISA檢測體系則具備優(yōu)異的線性范圍和檢測靈敏度。該抗體適用于探索DNA損傷應(yīng)答機制、腫瘤發(fā)生發(fā)展相關(guān)研究及放療敏感性評估等基礎(chǔ)科研領(lǐng)域，可支持石蠟包埋組織切片中蛋白表達(dá)模式分析、藥物處理前后細(xì)胞ATM激活狀態(tài)的動態(tài)監(jiān)測以及體外樣本的定量檢測需求。采用重組表達(dá)技術(shù)制備，具有批次穩(wěn)定性和高抗原結(jié)合特異性，為研究ATM在細(xì)胞周期調(diào)控和基因組穩(wěn)定性維持中的功能提供可靠工具。
Uniprot No.：

Q13315
基因名：

ATM
別名：

Serine-protein kinase ATM (EC 2.7.11.1) (Ataxia telangiectasia mutated) (A-T mutated), ATM
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from human ATM
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

3G11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FBXW7, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, UFL1, RAD9, UBQLN4 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks.
基因功能參考文獻(xiàn)：
1. In mitosis ATM forms a complex with the poly(ADP)ribose polymerase Tankyrase 1, the spindle pole protein NuMA1, and breast cancer protein BRCA1, another crucial DDR player. The complex is required for efficient poly(ADP)ribosylation of NuMA1. PMID: 24553124
2. ATM was a direct target of miR-181a. PMID: 24531888
3. A review discusses ATM function and the consequences of its loss during chronic lymphocytic leukemia pathogenesis. PMID: 23906020
4. Data show that serine-922 of TAX1BP2 protein is the phosphorylation site of ataxia telangiectasia mutated (ATM) kinase. PMID: 24240686
5. Data indicate that ataxia telangiectasia mutated (ATM) protein phosphorylates BRG1 protein at Ser-721. PMID: 24413084
6. These results indicate that ATM regulates a sub-set of NF-kappaB dependent genes after a genotoxic stress by direct phosphorylation of p65. PMID: 22715377
7. Data suggest that expression of cytomegalovirus UL76 up-regulates human interleukin-8 (IL8) expression/secretion in response to DNA damage; both UL76 and human ATM have roles in mechanism of IL8 induction during cytomegalovirus infection. PMID: 24068928
8. Huntington's disease cells presented a delayed nucleo-shuttling of phosphorylated forms of the ATM kinase. PMID: 24277524
9. ATM silencing induced partial reduction in levels of Skp2, a component of SCF(Skp2) ubiquitin ligase that controls Cdt1 degradation. PMID: 24280901
10. Heterozygosity for the ATM rs373759 polymorphism may be a potential risk factor for papillary thyroid cancer. PMID: 23925578
11. cAMP signaling inhibits radiation-induced ATM activation by PKA-dependent activation of PP2A, and this signaling mechanism augments radiation-induced apoptosis by reducing ATM-dependent activation of NF-kappaB in lung cancer cells. PMID: 24568192
12. Study found that in human oncogene-transformed and cancer cells, ATM suppressed ARF protein levels and activity in a transcription-independent manner. PMID: 23851489
13. Tumoral loss of ATM protein was detected more often in patients with a family history of pancreatic cancer than in those without PMID: 24486587
14. An important role of ATM-mediated Mad1 Serine 214 phosphorylation in mitosis. PMID: 24728176
15. OCT variants ( OCT1, OCT2 and ATM) were significantly associated with elevated baseline and glucose-induced C-peptide levels in polycystic ovary syndrome PMID: 24533710
16. The findings implicate an important role of variants in the ATM- CHEK2- BRCA1 axis in modification of the genetic predisposition to papillary thyroid carcinoma and its clinical manifestations. PMID: 24599715
17. Cuc B also triggers ATM-activated p53-14-3-3-sigma pathways. PMID: 24505404
18. Data indicate that ATM-depletion can sensitize breast cancer cells to PARP inhibition, suggesting a potential in the treatment of breast cancers low in ATM protein expression/activity, such as those arising in mutant ATM heterozygous carriers. PMID: 24252502
19. ATM levels were significantly down-regulated in oxaliplatin-resistant colorectal cancer cells. PMID: 24145123
20. ATM-mediated Snail Serine 100 phosphorylation in response to ionizing irradiation plays an important part in the regulation of radiosensitivity. PMID: 23891091
21. Our study indicates that the expression of Pim kinases is physiologically related to DNA-PKcs and ATM in ECs. PMID: 22282239
22. At a low reactive oxygen species condition during genotoxic insult, the ATM/sumoylated-IKKgamma interaction induced NFkappaB activation that resisted JNK-mediated apoptosis. PMID: 24457965
23. genetic association studies in a population of men in Germany: Data suggest that an SNP near ATM (ataxia telangiectasia mutated protein; rs11212617) is associated with coronary artery disease (but not blood glucose level) in the subjects studied. PMID: 24281401
24. ATM and ATR each contribute to DNA damage response (DDR) activation caused by BKPyV infection. PMID: 22952448
25. High ATM expression is associated with breast cancer. PMID: 23857602
26. Reduced protein expression of ATM is associated with breast carcinoma. PMID: 23117476
27. ATM protein expression is an independent prognostic marker in sporadic breast cancer. PMID: 24285016
28. ATM mutation and ATM protein loss had characteristics of old age, distal location of tumor, large tumor size, and histologic intestinal type. PMID: 24324828
29. ATM/ATR pathway plays an important role in tumor recognition. PMID: 24726882
30. data confirm previous work showing that Lig3 is required to maintain mtDNA integrity and function, and highlight a new function of ATM in regulating DNA Lig3 stability and consequently mtDNA repair PMID: 24342190
31. ATM mutations either alone or in combination with 11q deletion lead to demonstrable ATM dysfunction in patients with chronic lymphocytic leukemia. PMID: 23585524
32. The findings of this study suggested that biallelic ATM-inactivating mutations may present as isolated, generalized dystonia. PMID: 23640770
33. This study therefore sheds light on the mechanisms underlying AZA resistance, and will enable better understanding of AZA resistance in patients undergoing AZA treatment. PMID: 24680865
34. NKX3.1 and ATM have a functional interaction leading to ATM activation and then NKX3.1 degradation in a tightly regulated DNA damage response specific to prostate epithelial cells. PMID: 23890999
35. Activation of H2AX and ATM in varicella-zoster virus infected cells is associated with expression of VZV ORF61 and ORF63. PMID: 24606682
36. IP7, formed by IP6K2, binds CK2 to enhance its phosphorylation of the Tti1/Tel2 complex, thereby stabilizing DNA-PKcs and ATM. This process stimulates p53 phosphorylation at serine 15 to activate the cell death program. PMID: 24657168
37. ATM and MDC1 maintain genomic stability not only by controlling the DNA damage response, but also by regulating spindle assembly checkpoint activation, providing an important link between these two essential biological processes. PMID: 24509855
38. we elucidated the prognostic implications of the expressions of ATM, Chk2, and p53, in gastric carcinoma PMID: 23969480
39. The regulation of ATM by HDAC enzymes therefore suggests a vital role for HDAC1 and HDAC2 in the DNA damage response. PMID: 23939379
40. Apoptotic progression is markedly attenuated by ATM gene knockdown through downregulation of caspase-8 and caspase-9. PMID: 24530529
41. ATM and MAPKAP kinase 2 mediate radiation sensitivity in pancreatic cancer cells via phosphorylation of TRIM29. PMID: 24469230
42. data provide strong evidences that Aurora-A and BRCA1/2 inversely control the sensitivity of cancer cells to radio- and chemotherapy through the ATM/Chk2-mediated DNA repair networks PMID: 24480460
43. glioma stem cells were more resistant to radiation compared to glioma cells due to high expression of phosphorylated cell cycle checkpoint proteins, and inhibition of ATM could significantly reduce the radioresistance of glioma stem cells and glioma cells PMID: 23846672
44. ATM-deficient mice show resistance to hepatocyte cell death. PMID: 23435430
45. mimosine treatment, ATM blocks S phase entry in response to ROS, which prevents replication fork stalling-induced DNA damage. PMID: 24421316
46. In Rho 0-Hep G2 cells, which lack mitochondrial DNA and functional mitochondria, ATM failed to respond to hydrogen peroxide, indicating that mitochondria are required for the oxidative activation of ATM. PMID: 24406161
47. 12 pathogenic Atm mutations (1 missense, 4 nonsense, 5 frameshift, 1 splicing, and 1 large genomic deletion) were found in 8 Chinese patients from 5 families. All were novel. No homozygous mutation and founder-effect mutation were found. PMID: 23807571
48. Heterozygous carriers of c.8851-1G>T (associated with absence of ATM kinase activity) had a stronger radiosensitive phenotype with this assay than heterozygous carriers of p.Asp2708Asn (associated with residual kinase activity). PMID: 23632773
49. Significant association with PTC was found for rs1801516 (D1853N) in ATM and rs1867277 in the promoter region of FOXE1 (OR = 1.55, 95% CI 1.03, 2.34). PMID: 24105688
50. Kaposi's sarcoma-associated herpesvirus induces the ATM and H2AX DNA damage response early during de novo infection of primary endothelial cells, which play roles in latency establishment. PMID: 24352470
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相關(guān)疾病：

Ataxia telangiectasia (AT)
亞細(xì)胞定位：

Nucleus. Cytoplasmic vesicle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
蛋白家族：

PI3/PI4-kinase family, ATM subfamily
組織特異性：

Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
數(shù)據(jù)庫鏈接：

HGNC: 795

OMIM: 208900

KEGG: hsa:472

STRING: 9606.ENSP00000278616

UniGene: Hs.367437

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