ATF4 Recombinant Monoclonal Antibody

說明書

中文名稱：

ATF4重組抗體
貨號(hào)：

CSB-RA002272A0HU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
All lanes: ATF4 antibody at 1.6μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 KDa
Observed band size: 50 KDa

IHC image of CSB-RA002272A0HU diluted at 1:160 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-RA002272A0HU at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunoprecipitating ATF4 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA002272A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA002272A0HU (3μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)

Overlay histogram showing Hela cells stained with CSB-RA002272A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

ATF4重組單克隆抗體（CSB-RA002272A0HU）是一款針對(duì)人源ATF4蛋白的高特異性科研工具，適用于多種實(shí)驗(yàn)場(chǎng)景。ATF4（激活轉(zhuǎn)錄因子4）屬于堿性亮氨酸拉鏈轉(zhuǎn)錄因子家族，廣泛參與細(xì)胞應(yīng)激反應(yīng)、氨基酸代謝調(diào)控及內(nèi)質(zhì)網(wǎng)穩(wěn)態(tài)維持等關(guān)鍵生物學(xué)過程，尤其在氧化應(yīng)激和腫瘤微環(huán)境研究中具有重要價(jià)值。本抗體通過重組單克隆技術(shù)制備，經(jīng)ELISA、免疫印跡（WB）、免疫組化（IHC）、免疫熒光（IF）、流式細(xì)胞術(shù)（FC）和免疫沉淀（IP）等多平臺(tái)驗(yàn)證，展現(xiàn)出優(yōu)異的靈敏度和重復(fù)性。推薦實(shí)驗(yàn)稀釋比例為：WB 1:500-1:5000可清晰檢測(cè)目標(biāo)蛋白條帶，IHC 1:50-1:200適用于石蠟包埋組織切片定位，IF 1:20-1:200可實(shí)現(xiàn)亞細(xì)胞水平可視化，IP 1:200-1:1000則能高效富集內(nèi)源性ATF4復(fù)合物。該產(chǎn)品特別適用于研究細(xì)胞應(yīng)激信號(hào)通路、代謝重編程機(jī)制以及相關(guān)疾病模型的分子機(jī)制解析，為神經(jīng)退行性疾病、腫瘤微環(huán)境調(diào)控等基礎(chǔ)研究領(lǐng)域提供可靠工具。
Uniprot No.：

P18848
基因名：

ATF4
別名：

Activating transcription factor 4 antibody; ATF 4 antibody; ATF4 antibody; ATF4 protein antibody; ATF4_HUMAN antibody; cAMP-dependent transcription factor ATF-4 antibody; cAMP-responsive element-binding protein 2 antibody; CREB 2 antibody; CREB-2 antibody; CREB2 antibody; Cyclic AMP dependent transcription factor ATF 4 antibody; Cyclic AMP response element binding protein 2 antibody; Cyclic AMP-dependent transcription factor ATF-4 antibody; Cyclic AMP-responsive element-binding protein 2 antibody; DNA binding protein TAXREB67 antibody; DNA-binding protein TAXREB67 antibody; Tax Responsive Enhancer Element B67 antibody; Tax-responsive enhancer element-binding protein 67 antibody; TaxREB67 antibody; TXREB antibody
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from human ATF4
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號(hào)：

9E1
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF, FC, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200
IP	1:200-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
Immunoprecipitation (IP) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Transcription factor that binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3') and displays two biological functions, as regulator of metabolic and redox processes under normal cellular conditions, and as master transcription factor during integrated stress response (ISR). Binds to asymmetric CRE's as a heterodimer and to palindromic CRE's as a homodimer. Core effector of the ISR, which is required for adaptation to various stress such as endoplasmic reticulum (ER) stress, amino acid starvation, mitochondrial stress or oxidative stress. During ISR, ATF4 translation is induced via an alternative ribosome translation re-initiation mechanism in response to EIF2S1/eIF-2-alpha phosphorylation, and stress-induced ATF4 acts as a master transcription factor of stress-responsive genes in order to promote cell recovery. Promotes the transcription of genes linked to amino acid sufficiency and resistance to oxidative stress to protect cells against metabolic consequences of ER oxidation. Activates the transcription of NLRP1, possibly in concert with other factors in response to ER stress. Activates the transcription of asparagine synthetase (ASNS) in response to amino acid deprivation or ER stress. However, when associated with DDIT3/CHOP, the transcriptional activation of the ASNS gene is inhibited in response to amino acid deprivation. Together with DDIT3/CHOP, mediates programmed cell death by promoting the expression of genes involved in cellular amino acid metabolic processes, mRNA translation and the terminal unfolded protein response (terminal UPR), a cellular response that elicits programmed cell death when ER stress is prolonged and unresolved. Together with DDIT3/CHOP, activates the transcription of the IRS-regulator TRIB3 and promotes ER stress-induced neuronal cell death by regulating the expression of BBC3/PUMA in response to ER stress. May cooperate with the UPR transcriptional regulator QRICH1 to regulate ER protein homeostasis which is critical for cell viability in response to ER stress. In the absence of stress, ATF4 translation is at low levels and it is required for normal metabolic processes such as embryonic lens formation, fetal liver hematopoiesis, bone development and synaptic plasticity. Acts as a regulator of osteoblast differentiation in response to phosphorylation by RPS6KA3/RSK2: phosphorylation in osteoblasts enhances transactivation activity and promotes expression of osteoblast-specific genes and post-transcriptionally regulates the synthesis of Type I collagen, the main constituent of the bone matrix. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. Mainly acts as a transcriptional activator in cellular stress adaptation, but it can also act as a transcriptional repressor: acts as a regulator of synaptic plasticity by repressing transcription, thereby inhibiting induction and maintenance of long-term memory. Regulates synaptic functions via interaction with DISC1 in neurons, which inhibits ATF4 transcription factor activity by disrupting ATF4 dimerization and DNA-binding.; (Microbial infection) Binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
基因功能參考文獻(xiàn)：
1. phosphorylated PERK and ATF4 would be upregulated in Orexin neurons in Sudden Infant Death Syndrome (SIDS) compared to non-SIDS. PMID: 27796753
2. Our data suggests a novel interaction between Nrf2 and ATF4 under oxidative and endoplasmic reticulum stress, thus drives specific enzymatic and non-enzymatic reactions of antioxidant mechanisms maintaining redox homeostasis. PMID: 29421327
3. PSAT1, which is overexpressed in ER-negative breast cancers, is activated by ATF4 and promotes cell cycle progression via regulation of the GSK3beta/beta-catenin/cyclin D1 pathway. PMID: 29216929
4. POSTN may function as a protective factor for osteoblasts during this process by inhibiting the eIF2alphaATF4 pathway. PMID: 29207036
5. p62 directly targets nuclear transcription factors to control metabolic reprogramming in the microenvironment and repress tumorigenesis, and identifies ATF4 as a synthetic vulnerability in p62-deficient tumor stroma. PMID: 28988820
6. results suggest a conditional regulation of KRT16 gene by ATF4 that may be inhibited in normal cells, but engaged during cancer progression. Potential roles of KRT16, FAM129A and HKDC1 genes upregulation in adaptive stress responses and pathologies are discussed PMID: 29420561
7. Results provide evidence that the availability of glucose controls ATF4-mediated MITF suppression to drive melanoma cell proliferation. PMID: 28380427
8. Decreased ATF4 expression as a mechanism of acquired resistance to long-term amino acid limitation in cancer cells PMID: 28460466
9. These results suggest that p21 induction plays a vital role in the cellular response to ER stress and indicate that p21 is a prosurvival effector of ATF4. PMID: 28975618
10. GRP78 inhibition enhances ATF4-induced cell death by the deubiquitination and stabilization of CHOP in human osteosarcoma cells. PMID: 28947141
11. Expression of either dominant-negative or constitutively active mutants of Nrf2, ATF4, or c-Jun confirmed that distinct transcription units are regulated by these transcription factors. PMID: 27278863
12. ATF4 contributes to tumor growth of endometrial cancer (EC) by promoting CCL2 and subsequent recruitment of macrophage, and ATF4/CCL2 axis might be a potential therapeutic target for EC. PMID: 28843961
13. ATF4 expression fosters the malignancy of primary brain tumors and increases proliferation and tumor angiogenesis; experiments revealed that ATF4-dependent tumor promoting effects are mediated by transcriptional targeting the glutamate antiporter xCT PMID: 28553953
14. The PERK-eIF2alpha-ATF4-CHOP signaling pathway has a critical role in tumor progression during endoplasmic reticulum stress. (Review) PMID: 27211800
15. ATF4 pathway is activated in vivo upon mitochondrial stress. PMID: 28566324
16. a shortage of tryptophan caused by expression of indoleamine 2,3-dioxygenase (IDO) and tryptophan 2,3-dioxygenase (TDO) resulted in ATF4-dependent upregulation of several amino acid transporters. PMID: 27651314
17. SLC30A10 has a protective role in 1-methyl-4-phenylpyridinium-induced toxicity via PERK-ATF4 pathway. PMID: 28688763
18. There was decreased or loss of ATF4 in 52% of medullary thyroid cancer (MTC) tumors (n = 39) compared with normal thyroid follicle cells. A negative correlation was observed between RET and ATF4 protein levels in MTC tumors. PMID: 27935748
19. hypoxia-induced ATF4 expression may promote progression of proliferating infantile hemangioma through macrophage colony-stimulating factor induced M2-polarized macrophages infiltration. PMID: 28438094
20. overexpression of eIF5 and 5MP induces translation of ATF4. PMID: 27325740
21. ATF4 may exert various physiological roles in lipid metabolism depending on the nutrient composition. In addition, these results suggested that ATF4 has a role in regulating lipogenesis and in the development of NAFLD; thus ATF4 may be considered a therapeutic target for NAFLD. PMID: 27357269
22. PERK-eIF2alpha-ATF4 signaling pathway mediated by endoplasmic reticulum stress involved in osteoblast differentiation of periodontal ligament cells under cyclic mechanical force. PMID: 27079961
23. The localization of ATF4 in the granular component of nucleoli together with its association with nascent RNA transcripts in cells undergoing proteotoxic cell stress could suggest a new function for ATF4 in cell stress management. PMID: 27567537
24. The results showed that the FGF21 promoter contains three response elements for ATF4, suggesting that FGF21 is a sensitive target of ATF4. PMID: 27010621
25. ADM-2 is a stress-inducible gene controlled by ATF-4. PMID: 27328454
26. The results suggest that C12orf39, CSTA, and CALCB are novel ATF4 target genes, and that C12orf39 promoter activity is activated by ATF4 through amino acid response element. PMID: 26967115
27. High ATF4 expression is associated with osteosarcoma progression. PMID: 26797758
28. miR-214 directly targeted ATF4, a crucial transcriptional factor involved in anti-stress responses, down regulation of miR-214 releases the repression of ATF4 translation and leads to increased ATF4 protein content. PMID: 26791102
29. The activation of ATF4 in response to ONC201 required the kinases HRI and PKR, which phosphorylate and activate the translation initiation factor eIF2alpha. PMID: 26884600
30. TBL2 participates in ATF4 translation through its association with the mRNA. PMID: 26239904
31. Inhibition or overexpression of ATF4 confirms the role of ATF4 in SESN2 gene up-regulation induced by mitochondrial dysfunction. PMID: 26771712
32. ATF4 and ATF6beta act synergistically in the negative regulation of placental growth factor mRNA expression PMID: 26648175
33. Authors observed that a slow rate of ATF4-translation and late re-initiation of general translation coincided with cells which were resistant to ER stress-induced cell death. PMID: 25633195
34. a reduction of cell death was associated with decreased levels of ATF4 in a rhabdomyosarcoma cell line PMID: 26172539
35. Combined administration inhibited the cells most potently and time-dependently, decreased the expression of HO-1, and significantly increased the expression of ATF4, CHOP, and Ire-1 proteins expression levels PMID: 26125799
36. Global profiling in human mesenchymal stem cells and a novel cell-free assay reveals that ATF4 requires C/EBPbeta for genomic binding at a motif distinct from that bound by the C/EBPbeta homodimer. PMID: 26111340
37. This study outlines the mechanism of NIR laser phototoxicity and the utility of monitoring surface temperature and ATF4 expression as potential biomarkers to develop safe and effective clinical applications. PMID: 26030745
38. Up-regulation of ATF4 is associated with Pancreatic Neuroendocrine Tumors. PMID: 26504039
39. The ATF4/p75NTR/IL-8 signal pathway may have an important role in EndoMT induced by SFO. PMID: 24905361
40. ATF4 is a potential biomarker for esophageal squamous cell carcinoma (ESCC) prognosis and its dysregulation may play a key role in the regulation of invasion and metastasis in ESCC. PMID: 25078779
41. Upon loss of attachment in tumor cells, ATF4 activated a program of cytoprotective autophagy and antioxidant responses, including induced expression of heme oxygenase 1 (HO-1). Increased levels of HO-1 ameliorated oxidative stress and cell death. PMID: 26011642
42. Treatment with a skin sensitizer rapidly induces the phosphorylation of eIF2a and a concomitant increase of ATF4 protein levels in dendritic cells. PMID: 25236743
43. The results demonstrate that the endoplasmic reticulum stress-regulated ATF4/p16 pathway is involved in the premature senescence of renal tubular epithelial cells during diabetic nephropathy progression. PMID: 25567807
44. RET as a novel dual kinase with nuclear localization and provide mechanisms by which RET represses the proapoptotic genes PMID: 25795775
45. A sustained deficiency of mitochondrial respiratory complex III induces an apoptotic cell death through the p53-mediated inhibition of pro-survival activities of the ATF4. PMID: 25375376
46. ATF4 signaling pathway is essential for mediating the effect of ER stress on beta-klotho expression. PMID: 25727012
47. B-cell lymphoma/leukemia 10 promotes oral cancer progression through STAT1/ATF4/S100P signaling pathway. PMID: 24681956
48. ATF4-mediated repression of apelin contributes substantially to the pro-apoptotic effects of p38. PMID: 25052841
49. Bone diseases of diabetes mellitus type 2 showing definite changes of ATP4 gene expression. PMID: 24715035
50. the PERK/ATF4/LAMP3-arm of the UPR is an additional pathway mediating hypoxia-induced breast cancer cell migration PMID: 23294542
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亞細(xì)胞定位：

Nucleus. Nucleus speckle. Cytoplasm. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
蛋白家族：

BZIP family
數(shù)據(jù)庫鏈接：

HGNC: 786

OMIM: 604064

KEGG: hsa:468

STRING: 9606.ENSP00000336790

UniGene: Hs.496487

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