ALDOA Recombinant Monoclonal Antibody

說明書

中文名稱：

ALDOA重組抗體
貨號：

CSB-RA571479A0HU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, Mouse Liver whole cell lysate, Rat Liver whole cell lysate
All lanes: Aldolase antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40, 46 kDa
Observed band size: 40 kDa

IHC image of CSB-RA571479A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of Hela Cells with CSB-RA571479A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

ALDOA重組單克隆抗體（產(chǎn)品編號CSB-RA571479A0HU）是針對果糖二磷酸醛縮酶A（Aldolase A）研發(fā)的高特異性科研用抗體。ALDOLA是糖酵解通路的關(guān)鍵酶，參與細(xì)胞能量代謝調(diào)控，其表達(dá)異常與腫瘤增殖、代謝性疾病及肌肉相關(guān)病理過程密切相關(guān)。本抗體采用重組表達(dá)技術(shù)制備，經(jīng)多種實驗驗證顯示優(yōu)異性能：在Western Blot中能清晰檢測目標(biāo)蛋白條帶，推薦使用稀釋比為1:500-1:5000；免疫組化（IHC）實驗在1:50-1:200范圍內(nèi)可準(zhǔn)確顯示組織樣本中ALDOLA的定位分布；免疫熒光（IF）在1:20-1:200稀釋條件下呈現(xiàn)高信噪比的亞細(xì)胞定位信號，同時適用于ELISA檢測。適用于研究糖代謝重編程機(jī)制、腫瘤微環(huán)境能量供給、肌肉相關(guān)疾病模型等科研領(lǐng)域，為體外培養(yǎng)細(xì)胞、動物模型組織切片及蛋白樣本的分析提供可靠工具。該抗體具有批次穩(wěn)定性高、交叉反應(yīng)性低的特點，適用于多平臺實驗的標(biāo)準(zhǔn)化需求。
Uniprot No.：

P04075
基因名：

ALDOA
別名：

Fructose-bisphosphate aldolase A (EC 4.1.2.13) (Lung cancer antigen NY-LU-1) (Muscle-type aldolase), ALDOA, ALDA
反應(yīng)種屬：

Human, Mouse, Rat
免疫原：

A synthesized peptide derived from human Aldolase
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

7D8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
基因功能參考文獻(xiàn)：
1. Results showed that ALDOA was upregulated in renal cell carcinoma (RCC) samples and cell lines and significantly associated with metastasis and survival. Overall, data revealed that ALDOA functions as a tumor promoter, plays a prominent role in proliferation, migration, and invasion of RCC cells with high expression, and may promote EMT and activate the Wnt/betacatenin signaling pathway. PMID: 29693182
2. The results indicate that ALDOA and PGK1 may indicate resistance to cisplatin in osteosarcoma PMID: 29199648
3. Knockdown of ALDOA in QBC939 and RBE cells attenuated the cell proliferation and induced a higher apoptosis rate. PMID: 30121648
4. Silencing aldolase A suppressed colon cancer cell proliferation and invasion and inhibited the epithelial-mesenchymal transition phenotype. Aldolase A protein expression in colon cancer was related to tumor location, tumor clinical stage and survival. PMID: 29453983
5. mir-122 and its targets G6PC3, ALDOA and CS play roles in the hypoxia responses that regulate glucose and energy metabolism and can serve as hypoxia biomarkers. PMID: 27793029
6. Aldolase A promotes lung cancer metastasis via PHD-mediated stabilization of HIF-1alpha and the subsequent activation of MMP9. PMID: 28610954
7. Findings show that ALDOA expression is up-regulated in colorectal cancer (CRC) and is a hypoxia-inducible prognostic factor that is closely related to CRC malignancy. PMID: 28000858
8. Overexpression of ALDOA is associated with colorectal cancer. PMID: 27468721
9. In vitro and in vivo results demonstrated that ALDOA was associated with proliferation and metastasis of pancreatic cancer cells. PMID: 26854714
10. Study shows that PI3K directly coordinates glycolysis with cytoskeletal dynamics in an AKT-independent manner. Growth factors or insulin stimulate the PI3K-dependent activation of Rac, leading to disruption of the actin cytoskeleton, release of filamentous actin-bound aldolase A, and an increase in aldolase activity. PMID: 26824656
11. Our results expand the clinical spectrum of aldolase A deficiency to isolated temperature-dependent rhabdomyolysis, and suggest that thermolability may be tissue specific. We also propose a treatment for this severe disease PMID: 25392908
12. Study provides evidence supporting a critical functional role of ALDOA in osteosarcoma progression, metastasis and perhaps chemoresistance. PMID: 25215901
13. ALDOC, Aldolase A (ALDOA) and Aldolase B (ALDOB) activate Wnt signaling. PMID: 24993527
14. ALDOA is highly expressed in lung squamous cell carcinoma (LSCC) and its expression level is correlated with LSCC metastasis, grades, differentiation status and poor prognosis. PMID: 24465716
15. The expression of ALDOA and/or SULT1A3 is significantly higher. PMID: 22949271
16. The results presented here point to ALDA as a factor involved in the regulation of cells proliferation. PMID: 23886627
17. The new prognostic biomarkers GRP78, Fructose-bisphosphate Aldolase A (ALDOA), Carbonic Anhydrase I (CA1) and Peptidyl-prolyl cis-trans isomerase A or Cyclophilin A (PPIA)) provided good survival prediction for TNM stage I-IV patients. PMID: 22996014
18. ALDOA is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
19. Data that aldolase forms a complex with ARNO/Arf6 and the V-ATPase and that it may contribute to remodeling of the actin cytoskeleton. PMID: 21307348
20. Posttranslational nitration of aldolase A may be an important pathway that regulates mast cell phenotype and function. PMID: 20511553
21. ALDOA is involved in keratinocyte migration following the induction of lamellipodia formation, and ALDOA-related migration is enhanced by EGF PMID: 20362419
22. melanoma antigen expressed in G361, a representative melanoma cell line/ reacted with autoantibodies in patient sera PMID: 20181627
23. Gly346 is crucial for the correct conformation and function of aldolase A, because it governs the entry/release of the substrates into/from the enzyme cleft, and/or allows important C-terminal residues to approach the active site. PMID: 14766013
24. The existence of highly AMP-sensitive muscle-like FBPase, activity of which is regulated by metabolite-dependent interaction with aldolase enables the precise regulation of muscle energy expenditures. PMID: 18214967
25. Results identify VDAC2 and aldolase A as membrane proteins of K562 cells with increased expression under iron deprivation. PMID: 18278581
26. Aldolase A may play a role in the radio-response of human cells, probably in nuclei, in addition to its glycolytic role in the cytosol. PMID: 18328256
27. ZNF224 recruits the arginine methyltransferase PRMT5 on the transcriptional repressor complex of the aldolase A gene PMID: 19741270
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相關(guān)疾病：

Glycogen storage disease 12 (GSD12)
亞細(xì)胞定位：

Cytoplasm, myofibril, sarcomere, I band. Cytoplasm, myofibril, sarcomere, M line.
蛋白家族：

Class I fructose-bisphosphate aldolase family
數(shù)據(jù)庫鏈接：

HGNC: 414

OMIM: 103850

KEGG: hsa:226

STRING: 9606.ENSP00000336927

UniGene: Hs.513490

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