WNK1 Recombinant Monoclonal Antibody

說(shuō)明書

中文名稱：

WNK1重組抗體
貨號(hào)：

CSB-RA182028A0HU
規(guī)格：

￥1320
圖片：
IHC image of CSB-RA182028A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.60% DAB.

IHC image of CSB-RA182028A0HU diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.60% DAB.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

WNK1重組單克隆抗體（CSB-RA182028A0HU）是針對(duì)絲氨酸/蘇氨酸蛋白激酶WNK1開發(fā)的高特異性科研工具。WNK1作為細(xì)胞離子穩(wěn)態(tài)調(diào)節(jié)的關(guān)鍵因子，通過(guò)調(diào)控鈉鉀氯協(xié)同轉(zhuǎn)運(yùn)蛋白（NKCC）和上皮鈉通道（ENaC）活性參與血壓調(diào)節(jié)及腎臟電解質(zhì)平衡，其功能異常與遺傳性高血壓綜合征、慢性腎病等病理過(guò)程密切相關(guān)。本產(chǎn)品采用重組表達(dá)技術(shù)制備，經(jīng)過(guò)ELISA和免疫組化（IHC）雙重驗(yàn)證，在IHC應(yīng)用中可呈現(xiàn)清晰的組織特異性定位信號(hào)，推薦使用稀釋比例為1:50-1:200，適用于石蠟包埋組織切片的蛋白表達(dá)分析。該抗體具有高度批次穩(wěn)定性與抗原結(jié)合一致性，可滿足腎臟生理學(xué)、離子通道調(diào)控機(jī)制及高血壓相關(guān)疾病模型的研究需求，為探索WNK1在細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)和器官功能調(diào)控中的作用提供可靠檢測(cè)工具，特別適用于心血管及泌尿系統(tǒng)疾病基礎(chǔ)研究中的蛋白表達(dá)水平檢測(cè)與組織分布研究。
Uniprot No.：

Q9H4A3
基因名：

WNK1
別名：

Serine/threonine-protein kinase WNK1 (EC 2.7.11.1) (Erythrocyte 65 kDa protein) (p65) (Kinase deficient protein) (Protein kinase lysine-deficient 1) (Protein kinase with no lysine 1) (hWNK1), WNK1, HSN2 KDP KIAA0344 PRKWNK1
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from Human WNK1
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號(hào)：

26G7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.; Dominant-negative regulator of the longer isoform 1. Does not have kinase activity, does not directly inhibit WNK4 and has no direct effect on sodium and chloride ion transport. Downregulates sodium-chloride cotransporter activity indirectly by inhibiting isoform 1, it associates with isoform 1 and attenuates its kinase activity. In kidney, may play an important role regulating sodium and potassium balance.
基因功能參考文獻(xiàn)：
1. SNP rs1468326 of WNK1, rs6749447 of STK39, and WNK1 haplotype AGACAGGAATCGT were associated with hypertension in Tibetan individuals PMID: 28945285
2. A new WNK1/HSN2 founder mutation has been found in Japanese patients with hereditary sensory and autonomic neuropathy. PMID: 28422281
3. Small interfering RNA knockdown of MAPK7 demonstrated that MAPK7 regulates a subset of WNK1-regulated genes and controls the migration and cell proliferation PMID: 29025069
4. The AA genotype of WNK1 rs1468326 is related with an increased risk for preeclampsia. PMID: 29777907
5. Mutated WNK1 is a driver of chronic lymphocytic leukemia. PMID: 28679620
6. Using small-interfering RNA-mediated WNK1 knockdown, we show autophagosome formation and autophagic flux are accelerated. In cells with reduced WNK1, basal and starvation-induced autophagy is increased. We also show that depletion of WNK1 stimulates focal class III phosphatidylinositol 3-kinase complex (PI3KC3) activity, which is required to induce autophagy. PMID: 27911840
7. WNK1 and the associated phosphorylation of the PCF11 CID act to promote transcript release from chromatin-associated Pol II, which in turn facilitates mRNA export to the cytoplasm PMID: 29196535
8. Akt3 constitutively suppresses macropinocytosis in macrophages through a novel WNK1/SGK1/Cdc42 pathway. PMID: 28389565
9. NKCC1 and KCCs are coordinately regulated by L-WNK1 isoforms. PMID: 27170636
10. Single nucleotide polymorphisms STK39 and WNK were associated with hypertension and BP in our multicenter Belgian case-control study PMID: 27082544
11. Identify a new chimeric transcript generated by the fusion of WNK1 and B4GALNT3 genes, correlated with B4GALNT3 overexpression in papillary thyroid carcinoma. PMID: 25803323
12. a novel gene, WNK1, for susceptibility to pelvic organ prolapse PMID: 25739019
13. findings indicate that the proline-rich exons are modular cassettes that convert WNK1 into a NEDD4-2 substrate, thereby linking aldosterone and other NEDD4-2-suppressing antinatriuretic hormones to NCC phosphorylation status. PMID: 26241057
14. enhances BK channel function by reducing ERK1/2 signaling-mediated lysosomal degradation of the channel PMID: 25145935
15. conclude that although multiple candidate genes are involved in development of hypertension, the genetic polymorphism in WNK1 is not a major contributor to the observed variability in blood pressure and familial clustering risk of hypertension PMID: 25321950
16. study identifies a separation of functions for the WNK1-activated protein kinases OSR1 and SPAK in mediating proliferation, invasion, and gene expression in endothelial cells PMID: 25362046
17. Report generation of WNK1 knockout cell lines and effects on WNK signaling. PMID: 25477473
18. data suggest that WNK1 functions as a chloride sensor through direct binding of a regulatory chloride ion to the active site, which inhibits autophosphorylation PMID: 24803536
19. our data show a novel role for the WNK1/OSR1/NKCC1 pathway in glioma migration PMID: 24555568
20. Findings suggest that inactivating mutations in WNK1 may cause hypokalemic salt-losing tubulopathies (SLT). PMID: 22934535
21. Wnk kinases are positive regulators of canonical Wnt/beta-catenin signaling. PMID: 23797875
22. The WNK1 gene might be mechanistically involved in the variation in BP response to dietary sodium and potassium intake among individuals, and might contribute to the variation of this complex phenotype. PMID: 23059770
23. Data show that intracellular association between WNK1 and oxidative stress-responsive 1 (OSR1) is required for stimulation of OSR1 and Na(+), K(+), Cl(-)-Cotransporter NKCC1 and NKCC2 activities by osmotic stress. PMID: 22989884
24. The results evidenced a striking tissue-specific distribution of the different isoforms and the unexpected presence of exon HSN2 in many tissues other than the nervous system. PMID: 22701532
25. review focuses on the mechanisms by which deletions of the first intron of WNK1 found in pseudohypoaldosteronism type 2 patients trigger the disease PMID: 22080857
26. WNK1 stimulates PLC-beta signaling in cells by promoting the synthesis of PIP2 via stimulation of phosphatidylinositol 4-kinase IIIalpha. PMID: 22119528
27. hypertension associated polymorphisms in WNK1 and WNK4 may not be predictors for antihypertensive response to diuretics. PMID: 21704025
28. we report a novel mutation in the WNK1/HSN2 gene in HSAN2 disease PMID: 21625937
29. WNK1 AluYb8 insertion might affect human blood pressure via altering the profile of alternatively spliced transcripts PMID: 21520334
30. These results suggested that WNK1 polymorphisms were involved in the predisposition of essential hypertension in Hani and Yi populations and its effects showed a clear population specificity. PMID: 21530900
31. Both IRBIT (inositol 1,4,5-trisphosphate receptor-binding protein) and WNK [with no lysine (K)] kinase have been implicated as additional HCO(3)(-) secretory controllers. PMID: 21242704
32. PI3K-activating hormones inhibit ROMK by enhancing its endocytosis via a mechanism that involves phosphorylation of WNK1 by Akt1 and SGK1. PMID: 21355052
33. Data show that a large percentage of WNK1 knockdown cells fail to complete cell division, displaying defects in mitotic spindles and also in abscission and cell survival. PMID: 21220314
34. IRBIT opposes the effects of WNKs and SPAK by recruiting PP1 to the complex to dephosphorylate CFTR and NBCe1-B, restoring their cell surface expression, in addition to stimulating their activities PMID: 21317537
35. WNK1 promotes cell surface expression of glucose transporter GLUT1 by regulating a Tre-2/USP6-BUB2-Cdc16 domain family member 4 (TBC1D4)-Rab8A complex PMID: 20937822
36. Three different truncating mutations of HSN2 is associated with hereditary sensory and autonomic neuropathy PMID: 15060842
37. Screening of hsn2 gene in an HSAN type II Lebanese family showed a 1bp deletion mutation found in a homozygous state in all affected individuals. PMID: 15455397
38. Two founder mutations are responsible for the apparently higher prevalence of HSAN2 in French Canadians. Genotype-phenotype correlation does not suggest any significant clinical variability. PMID: 15911806
39. HSN2 mutations are associated with an early childhood onset of a predominantly sensory neuropathy. PMID: 16534117
40. HSN2 is a nervous system-specific exon of the WNK1 gene (WNK1/HSN2 isoform). PMID: 18521183
41. identification of autoinhibitory domain PMID: 12374799
42. Protein kinase B mediates the phosphorylation of WNK1 at Thr-60. PMID: 14611643
43. control of kidney WNK1 gene expression of kinase-active or -deficient isoforms is mediated predominantly through the use of multiple transcription initiation sites PMID: 14645531
44. WNK1 selectively binds to and phosphorylates synaptotagmin 2 (Syt2) within its calcium binding C2 domains. Endogenous WNK1 and Syt2 coimmunoprecipitate and colocalize on a subset of secretory granules in INS-1 cells. PMID: 15350218
45. stimulation of KS-WNK1 expression might be an important element of aldosterone-induced Na(+) retention and hypertension PMID: 15583131
46. WNK kinase may be able to influence ion homeostasis through its effects on synaptotagmin function (review) PMID: 15686619
47. WNK1 is activated by hypertonic stress. WNK1 phosphorylated both WNK4 and WNK2. In addition, the WNK1 autoinhibitory domain inhibited the catalytic activity of these WNKs. PMID: 15883153
48. Polymorphisms in genes regulating renal sodium transport, in particular WNK1, predict interindividual differences in antihypertensive responses to hydrochlorothiazide. PMID: 16172412
49. WNK1 and SPAK/OSR1 mediate the hypotonic stress signaling pathway to cation-chloride-coupled cotransporters PMID: 16263722
50. role of WNK1 in blood pressure regulation; interactions of functional variants of WNK1 with dietary intake or with response to antihypertensive drugs, and their impact on cardiovascular morbidity and mortality PMID: 16301342
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相關(guān)疾?。?/div>
Pseudohypoaldosteronism 2C (PHA2C); Neuropathy, hereditary sensory and autonomic, 2A (HSAN2A)
亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

Protein kinase superfamily, Ser/Thr protein kinase family, WNK subfamily
組織特異性：

Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 14540

OMIM: 201300

KEGG: hsa:65125

STRING: 9606.ENSP00000313059

UniGene: Hs.744906

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