TMEM192 Recombinant Monoclonal Antibody
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中文名稱(chēng)：TMEM192重組抗體
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貨號(hào)：CSB-RA249639A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: HepG2 whole cell lysate, PC-3 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate
All lanes: TMEM192 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa -
IHC image of CSB-RA249639A0HU diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CSB-RA249639A0HU TMEM192重組單克隆抗體是針對(duì)跨膜蛋白192（TMEM192）研發(fā)的高特異性科研工具，適用于蛋白質(zhì)定位及功能機(jī)制研究。TMEM192作為溶酶體相關(guān)跨膜蛋白，在細(xì)胞器穩(wěn)態(tài)維持、物質(zhì)運(yùn)輸及代謝調(diào)控中發(fā)揮重要作用。該抗體經(jīng)多種技術(shù)平臺(tái)驗(yàn)證，在Western Blot（WB）中可識(shí)別天然狀態(tài)靶蛋白，呈現(xiàn)單一清晰條帶；免疫組化（IHC）實(shí)驗(yàn)顯示其在多種組織樣本中能精準(zhǔn)定位TMEM192的亞細(xì)胞分布，染色結(jié)果呈現(xiàn)優(yōu)異的分辨率和特異性。推薦使用稀釋度為WB 1:500-1:2000，IHC 1:50-1:200，實(shí)驗(yàn)數(shù)據(jù)展現(xiàn)優(yōu)異的批次間穩(wěn)定性和重復(fù)性。本產(chǎn)品適用于探索溶酶體生物學(xué)功能、細(xì)胞器互作網(wǎng)絡(luò)分析、以及疾病模型中跨膜蛋白異常表達(dá)研究等領(lǐng)域，為細(xì)胞生物學(xué)、分子病理學(xué)及代謝調(diào)控機(jī)制等基礎(chǔ)研究提供可靠檢測(cè)工具，嚴(yán)格限于體外實(shí)驗(yàn)及科學(xué)研究使用。
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Uniprot No.：
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基因名：TMEM192
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別名：Transmembrane protein 192, TMEM192
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反應(yīng)種屬：Human
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免疫原：A synthesized peptide derived from human TMEM192
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類(lèi)型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號(hào)：21G8
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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靶點(diǎn)詳情
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基因功能參考文獻(xiàn)：
- Authors found that transmembrane protein 192 (TMEM192) interacted with TIG1. Authors also found that both TIG1A and TIG1B isoforms interacted and co-localized with TMEM192 in HtTA cervical cancer cells. The expression of TIG1 induced the expression of autophagy-related proteins. PMID: 27989102
- TMEM192 deficiency can induce autophagy in tumor cells, and can further activate apoptosis by the mitochondrial pathway through autophagy. PMID: 22736246
- TMEM192 was found to be strongly expressed in human kidney, liver, lung and pancreas tissue. The widespread tissue distribution could suggest an important role of TMEM192 for lysosomal function. PMID: 20370317
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亞細(xì)胞定位：Lysosome membrane; Multi-pass membrane protein. Late endosome.
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蛋白家族：TMEM192 family
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組織特異性：Strongly expressed in kidney, liver, lung and pancreas.
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數(shù)據(jù)庫(kù)鏈接：
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