TK1 Recombinant Monoclonal Antibody

中文名稱(chēng)：

TK1重組抗體
貨號(hào)：

CSB-RA931459A0HU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in: 293T whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate
All lanes: Thymidine Kinase 1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa

Immunofluorescence staining of Hela with CSB-RA931459A0HU at 1:40, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 491-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing Hela cells stained with CSB-RA931459A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

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產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA931459A0HU TK1重組單克隆抗體是針對(duì)人源胸苷激酶1（Thymidine Kinase 1, TK1）研發(fā)的高特異性檢測(cè)工具。TK1是DNA合成關(guān)鍵酶，通過(guò)催化胸苷磷酸化參與細(xì)胞增殖調(diào)控，其表達(dá)水平與細(xì)胞周期進(jìn)程密切相關(guān)，在腫瘤發(fā)生、細(xì)胞代謝等領(lǐng)域具有重要研究?jī)r(jià)值。本抗體經(jīng)多種平臺(tái)驗(yàn)證，適用于Western Blot（1:500-1:2000）、免疫熒光（1:50-1:200）、流式細(xì)胞術(shù)（1:50-1:200）及ELISA檢測(cè)，實(shí)驗(yàn)數(shù)據(jù)顯示其能特異性識(shí)別天然構(gòu)象和變性狀態(tài)的TK1蛋白，在WB中呈現(xiàn)單一清晰條帶，免疫熒光實(shí)驗(yàn)可精準(zhǔn)定位細(xì)胞質(zhì)內(nèi)靶蛋白分布，流式檢測(cè)則有效解析細(xì)胞群體中TK1的表達(dá)差異。該重組單克隆抗體采用哺乳動(dòng)物表達(dá)系統(tǒng)制備，具有高批次穩(wěn)定性和低交叉反應(yīng)性，適用于細(xì)胞周期調(diào)控機(jī)制研究、腫瘤細(xì)胞增殖能力評(píng)估、抗腫瘤藥物體外篩選等科研場(chǎng)景，為探索TK1在疾病模型中的功能提供可靠工具。本產(chǎn)品嚴(yán)格限定于體外研究使用。
Uniprot No.：

P04183
基因名：

TK1
別名：

Thymidine kinase, cytosolic (EC 2.7.1.21), TK1
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from Human TK1
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類(lèi)型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號(hào)：

6F6
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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基因功能參考文獻(xiàn)：
1. STK1p is a potential proliferating biomarker for early discover of persons in the risk to develop or already have pre-malignancies or diseases associated with the risk process of malignancy. PMID: 29689706
2. Data suggest that serum thymidine kinase 1 (S-TK) activity may be a useful parameter for monitoring neoadjuvant therapy (nTx) efficacy. PMID: 29189449
3. Our study provides the first evidence that serum TK1 activity as early as 2 weeks following CDK4/6 inhibitors is highly correlated with tumor cell proliferation response in patients with early-stage HR+ breast cancer. PMID: 29162134
4. TK1 might be involved in the development and progression of PDAC by regulating cell proliferation. PMID: 29266545
5. The detection of TK1 combined with that cytokeratin-19 fragment (CYFRA21-1), CEA or NSE increased the diagnostic value of TK1 for lung squamous cell carcinoma, adenocarcinoma and small cell lung cancer, respectively. PMID: 29247745
6. The results have implications for the mechanism by which Fhit regulates TK1 mRNA, and more broadly, for its modulation of multiple functions as tumor suppressor/genome caretaker. PMID: 28093273
7. Results suggest that positive expression of CK19 mRNA and TK1 protein is closely correlated with poor prognosis in advanced gastrointestinal cancer. PMID: 27625087
8. though TK1 expression was an independent prognostic factor for relapse, but not for survival, TK1 is a more informative expression than Ki-67 for LI, relapse and overall survival rates. Thus, when TK1 is combined with MDACC grading, pTNM staging and lymph node metastasis, IHC determination of TK1 expression may improve the overall prediction of prognosis in patients with ovarian cancer. PMID: 28651488
9. Data show that the median thymidine kinase (TK1) levels found in sera from breast cancer patients with T1 to T4 stage disease were 0.31, 0.46, 0.47, and 0.55 ng/ml, and these levels significantly differed from healthy controls. PMID: 27079872
10. STK1 is a reliable biomarker for discovering people with malignant tumors in cancer screening. PMID: 27002755
11. Serum Thymidine Kinase 1 Activity Following Nephrectomy for Renal Cell Carcinoma and Radiofrequency Ablation of Metastases to Lung and Liver PMID: 27069161
12. TK1 expression is significantly different in invasive urothelial carcinoma and benign urothelium, which underlines its potential as a diagnostic marker PMID: 26231311
13. Protein expression of BIRC5, TK1, and TOP2A in malignant peripheral nerve sheath tumours--A prognostic test after surgical resection. PMID: 25769404
14. High Thymidine Kinase expression is associated with adenocarcinoma in Non-small Cell Lung Cancer. PMID: 25921119
15. These results demonstrate that there are differences in the specific activities and the subunit compositions of STK1 in hematological malignancies compared with breast and prostate cancer. PMID: 25881026
16. A regression analysis showed that only TK1 levels were significant (relative risk (RR)=1.03 for each unit, confidence interval (CI)=1-1.05; p=0.015) for diagnosing a true transformation PMID: 25964590
17. We suggest that a chip including DPYD, TYMS, TYMP, TK1, and TK2 genes is a potential tool to predict response in LARC following fluoropyrimidine-based CCRT. PMID: 24455740
18. Nuclear TK1 expression in early grade cervical intraepithelial neoplasia predicts risk for progression to malignancy PMID: 23693054
19. 3'-deoxy-3'-[18F]-fluorothymidine (18F-FLT) kinetics correlates with thymidine kinase-1 expression and cell proliferation in newly diagnosed gliomas PMID: 23229746
20. The magnitude of maximum fluorodeoxyglucose uptake in primary tumors and the serum TK1 level in patients with metastatic NSCLC were found to be independent prognostic predictors of overall survival. PMID: 23116493
21. Increased serum level of thymidine kinase 1 correlates with metastatic site in patients with malignant melanoma. PMID: 23179401
22. results suggest that the serum TK1 protein differs from cellular or recombinant forms, is more active in high molecular weight complexes, and is sensitive to reducing agents PMID: 22741536
23. Data suggest that serum thymidine kinase 1 (TK1) levels may help to refine risk assessment in the modern immunotherapy era. PMID: 22263569
24. The crystal structure of the T163S-mutated HuTK1 reveals a less ordered conformation of the ligand thymidine triphosphate compared with the wild-type structure PMID: 22385435
25. TK1 may be involved in poor survival in patients with pT1 of lung adenocarcinoma PMID: 22143937
26. frequencies of polymorphic mutations in HIV-1 (subtype B) were compared between patients detected with the 69 insertion (n = 17), Q151M (n = 29), >/=2 thymidine analogue mutations (TAM) 1 (n = 400) or >/=2 TAM 2 (n = 249). PMID: 22027876
27. data demonstrate that the Flt3L/TK gene therapeutic approach can induce systemic immunological memory capable of recognizing a brain tumor neoantigen in a model of recurrent GBM PMID: 21505426
28. (18)F-FLT uptake and retention within cells may be complicated by a variety of still undetermined factors in addition to TK1 enzymatic activity PMID: 21764789
29. Elevated serum thymidine kinase 1 is associated with pre/early cancerous progression. PMID: 21545220
30. High levels of HER2 and Ki-67 or TK1 expression correlate with the increase of tumor grades and tumor recurrence in meningiomas. PMID: 20450760
31. Serological thymidine kinase 1 is a useful marker for prognosis in patients with esophageal, cardial and lung carcinomas. PMID: 20479645
32. Serum TK1 correlates to clinical stages and clinical reactions and monitors the effect of tumor therapies, not only in controlled clinical trials, but also in routine clinical settings. PMID: 20354751
33. direct involvement of the G-quadruplex motif in transcription of TK1 PMID: 20849417
34. nucleoside recognition mechanisms for TK1 and TK2 are very different. nonpolar nucleosides are likely to be active in the nucleotide salvage pathway in human cells. PMID: 20560637
35. Results suggest that higher thymidine levels in the TK- cells caused by defect thymidine salvage to dTTP protects against UV irradiation. PMID: 20544518
36. The expression of TK1 in tumor tissues correlated to pathological stages and clinical grades of carcinomas (ca) of esophagus, lung and in premalignancy of breast ductal ca. STK1p could monitor the out-come of tumor therapy. PMID: 20544519
37. more dTTP synthesis via TK1 take place after genotoxic insults in tumor cells, improving DNA repair during G(2) arrest. PMID: 20554529
38. cell cycle regulation of TK1 in normal tubule cells differ from that in other type of normal and malignant renal cells. PMID: 19957115
39. Thymidine kinase-1 and thymidylate synthase expression was markedly different between cancer types, suggesting that response to TAS-102 may differ. PMID: 20372850
40. thymidine kinase has a role in progression of lung cancer PMID: 20592392
41. TK1 in serum may possess a reference value in the evaluation of treatment and prognosis of non-Hodgkin's lymphoma following chemotherapy. PMID: 20140744
42. The TK1 model presented supports both K and k positive cooperativity. Three-parameter mass action models can and should replace the 3-parameter Hill model. PMID: 20003201
43. TK1 gene expression together with TS, TP and DPD gene expression may play important roles in influencing the malignant behavior of epithelial ovarian cancer. PMID: 11992400
44. Mutation analysis in the coding sequence of thymidine kinase 1 in breast and colorectal cancer PMID: 12699056
45. Long-term treatment of H9 human lymphoid cells in the presence of dideoxycytidine down-regulated TK1 gene expression and reduced the expression and activity of TK in resistant cells. PMID: 14659972
46. the enzymatic function at the G2/M phase of TK1 depends on its quaternary structure PMID: 14697231
47. activation of the APC/C-Cdh1 complex during mitotic exit controls timing of TK1 destruction PMID: 14701726
48. Activity of thymidine kinase, thymidine phosphorylase and thymidilate synthase in human cancer xenografts to investigate the contribution of these enzymes to the sensitivity of TAS-102. PMID: 14719072
49. The importance of valine 106 for the structure and function of TK1. PMID: 15153115
50. activation of TK1 may be critical to modulate the radiation-induced cell death and cell cycle progression in irradiated K562 cells. PMID: 15353126
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亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

Thymidine kinase family
數(shù)據(jù)庫(kù)鏈接：

HGNC: 11830

OMIM: 188300

KEGG: hsa:7083

STRING: 9606.ENSP00000301634

UniGene: Hs.515122

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