TFG Recombinant Monoclonal Antibody
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中文名稱：TFG Recombinant Monoclonal Antibody
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貨號(hào)：CSB-RA110287A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate(30μg), U-87MG whole cell lysate(30μg), HT-29 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), Hela whole cell lysate(30μg), Jurkat whole cell lysate(30μg), PC-3 whole cell lysate(30μg), Mouse brain tissue lysate(30μg), Rat brain tissue lysate(30μg)
All lanes: TFG antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 43 kDa
Observed band size: 56 kDa
Exposure time：2min -
IHC image of CSB-RA110287A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Immunofluorescence staining of A431 cell with CSB-RA110287A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing HepG2 cells stained with CSB-RA110287A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產(chǎn)品詳情
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Uniprot No.：
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基因名：TFG
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別名：Protein TFG (TRK-fused gene protein), TFG
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反應(yīng)種屬：Human, Mouse, Rat
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免疫原：A synthesized peptide from human TFG protein
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號(hào)：12E2
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Plays a role in the normal dynamic function of the endoplasmic reticulum (ER) and its associated microtubules. Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus.
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基因功能參考文獻(xiàn)：
- Differences in the severity of the disorder as well as new clinical findings. These include presence of clonus, undeveloped speech, and sleep disturbances. these findings extend the phenotypic spectrum associated with the TFG mutations in Hereditary spastic paraplegia. PMID: 28124177
- TFG organizes transitional ER (tER) and ER exit sites (ERESs) into larger structures. PMID: 27184855
- Results identified two TFG variants associated with hereditary spastic paraplegias (HSP) (c.316C>T and c.317G> A) confirming the causal nature of bi-allelic TFG mutations for HSP, and suggest that that mitochondrial impairment represents a pathomechanistic link to other neurodegenerative conditions. PMID: 27492651
- We established a genetic diagnosis in six families with autosomal recessive HSP (SPG11 in three families and TFG/SPG57, SACS and ALS2 in one family each). A heterozygous mutation in a gene involved in an autosomal dominant HSP (ATL1/SPG3A) was also identified in one additional family. Six out of seven identified variants were novel. PMID: 27601211
- This study finding p.Gly269Val in a newly identified Iranian pedigree affected with hereditary motor and sensory neuropathy with proximal predominance. PMID: 27653917
- HMSN-P caused by p.Pro285Leu mutation in TFG is not confined to patients with Far East ancestry. PMID: 25725944
- TFG functions at the endoplasmic reticulum (ER)/ER-Golgi intermediate compartments (ERGIC) interface to locally concentrate COPII-coated transport carriers and link exit sites on the ER to ERGIC membranes. PMID: 25586378
- TRIM68 targets TFG, a novel regulator of IFN production, and in doing so turns off and limits type I IFN production in response to anti-viral detection systems PMID: 24999993
- TFG plays an important role in the protein secretory pathways that are essential for proper functioning of the human peripheral nervous system. PMID: 25098539
- Study demonstrates that TFG1 physiologically functions to inhibit the protein degradation system, resulting in an increase in ER resident proteins and ER stress; the P285L mutant substantially enhances these consequences PMID: 24613659
- TFG plays a pivotal role in negative regulation of RNA-sensing, RIG-I-like receptor (RLR) family signaling pathways. PMID: 23810392
- Whole-exome sequencing reveals that HMSN-P is caused by a mutation in the TRK-fused gene on chromosome 3q13.2 PMID: 23553329
- Inhibition of TFG function causes hereditary axon degeneration by impairing endoplasmic reticulum structure. PMID: 23479643
- results suggest that the oncogenic effect of the t(3;9) translocation may be due to the TFG-TEC chimeric protein and that fusion of the TFG (NTD) to the TEC protein produces a gain-of-function chimeric product PMID: 22581839
- The TRK-fused gene is mutated in hereditary motor and sensory neuropathy with proximal dominant involvement. PMID: 22883144
- Mutations in TFG may have important clinical relevance for current therapeutic strategies to treat metastatic melanoma. PMID: 22250051
- A polymorphic gene fusion consisting of TRK-fused gene and G-protein-coupled receptor 128 is identified in healthy individuals and in patients with lymphoma and soft tissue neoplasms. PMID: 19797732
- TFG was fused to NOR1 is a patient with extraskeletal myxoid chondrosarcoma. PMID: 15188455
- TFG is a novel protein able to modulate SHP-1 activity. PMID: 15557341
- TFG enhances the effect of TNF-alpha, TANK, TNF receptor-associated factor (TRAF)2, and TRAF6 in inducing NF-kappaB activity; it is suggested that TFG is a novel member of the NF-kappaB pathway PMID: 16547966
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相關(guān)疾病：Neuropathy, hereditary motor and sensory, Okinawa type (HMSNO); Spastic paraplegia 57, autosomal recessive (SPG57)
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亞細(xì)胞定位：Endoplasmic reticulum.
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組織特異性：Ubiquitous.
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