TAGLN Recombinant Monoclonal Antibody

說明書

中文名稱：

TAGLN Recombinant Monoclonal Antibody
貨號：

CSB-RA023105MA1HU
規格：

￥1320
圖片：
Overlay Peak curve showing Hela cells stained with CSB-RA023105MA1HU (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was mouse IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA023105MA1HU TAGLN重組單克隆抗體是針對Transgelin（TAGLN）靶點開發的高特異性科研試劑。TAGLN是一種肌動蛋白結合蛋白，廣泛參與細胞骨架動態調節、平滑肌分化及細胞遷移等過程，其表達異常與血管重塑、纖維化疾病及腫瘤微環境調控密切相關。本抗體經ELISA與流式細胞術（FC）嚴格驗證，可特異性識別天然及重組TAGLN蛋白，推薦FC實驗使用稀釋比例為1:50-1:200，實驗結果呈現高信噪比與穩定性。該產品適用于平滑肌細胞功能研究、腫瘤細胞遷移與侵襲機制分析、以及病理狀態下細胞骨架重構等領域的體外實驗，為探索TAGLN在組織修復、疾病模型中的分子作用提供可靠工具。本產品僅供科學研究使用，不涉及臨床及相關應用場景。
Uniprot No.：

Q01995
基因名：

TAGLN
別名：

TAGLN antibody; SM22 antibody; WS3-10 antibody; Transgelin antibody; 22 kDa actin-binding protein antibody; Protein WS3-10 antibody; Smooth muscle protein 22-alpha antibody; SM22-alpha antibody
反應種屬：

Human
免疫原：

Recombinant Human TAGLN protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

mIgG2a
純化方式：

Affinity-chromatography
克隆號：

13G7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, FC

推薦稀釋比：

Application	Recommended Dilution
FC	1:50-1:200

Protocols：

ELISA Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Actin cross-linking/gelling protein. Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
基因功能參考文獻：
1. High expression level of TAGLN is associated with prostate cancer. PMID: 26934553
2. this study identified potential biochemical players involved in distant recurrence and indicates that R-Ras and Transgelin are potential post-surgical prognostic biomarkers for Stage III colorectal cancer PMID: 27270312
3. During transition from the pluripotent stage towards the neural developmental stage, TAGLN is differentially expressed in bipolar patient derived cells compared to control derived cells. PMID: 28117838
4. Data (including data from studies using cells cultured from transgenic/knockout mice) suggest that expression and degradation of transgelin in myofibroblasts and keratinocytes are regulated by mechanical tension in cytoskeleton produced by myosin II motor in response to stiffness of culture matrix/extracellular matrix. PMID: 28898058
5. transgelin (TAGLN), a transforming growth factor beta (TGFbeta)-inducible gene, was identified as an upregulated gene during in vitro osteoblastic and adipocytic differentiation of human bone marrow-derived stromal (skeletal) stem cells PMID: 27490926
6. Smooth muscle 22 regulates the proliferation of aortic smooth muscle cells participates in the development of thoracic aortic dissection. PMID: 27320219
7. regulates vasculogenic mimicry in breast cancer cells by enhancing interleukin-8 uptake PMID: 28058861
8. Serum concentrations of CK-18 fragments and transgelin-2 correlate with the severity of NAFLD, but not with obesity. PMID: 27740519
9. Increases or decreases in transgelin levels have reciprocal effects on tumor cell behavior, with higher expression promoting metastasis PMID: 26847345
10. activated AKT and JNK signaling pathways promote the overexpression of transgelin PMID: 26694173
11. Cofilin-1 and transgelin may play roles in the carcinogenesis and development of esophageal squamous cell carcinoma PMID: 26344167
12. Both tissue and salivary transgelin mRNA were closely correlated with various important clinicopathological parameters and were independent prognosis factors for oral squamous cell carcinoma. PMID: 26242444
13. SM22alpha is a phosphorylation-regulated suppressor of IKK-IkappaBalpha-NF-kappaB signaling cascades. PMID: 25937534
14. EZH2 regulates the chromatin structure at the TAGLN promoter through tri-methylation of H3K27, acting as an epigenetic integrator of IL-1beta and TGFbeta2 signaling. PMID: 25917318
15. Transgelin may be an excellent diagnostic marker of Triple negative tumors and could be useful in stratification of patients PMID: 25841305
16. Results indicate that miR-144 may regulate osteosarcoma cell proliferation and invasion by downregulating its target gene, transgelin protein (TAGLN), suggesting that miR-144 may be a potential therapeutic target for the treatment of osteosarcoma. PMID: 25318625
17. TAGLN was highly expressed in NF1-deficient malignant peripheral nerve sheath tumors compared to NF1-deficient plexiform neurofibromas. Upregulation was caused by increased transcriptional expression. The TAGLN gene was hypomethylated in the MPNST cells. PMID: 25109740
18. our data suggest that TAGLN may be a viable therapeutic target and a potential biomarker for predicting the prognosis of patients with lung adenocarcinoma. PMID: 24938684
19. The expression and biological role of transgelin seem to differ among various types of tumor cells and stroma, and possibly change during tumor progression. PMID: 24476357
20. They also antagonized the TGF-beta1 induced up-regulation of CTGF and transgelin. PMID: 24828686
21. Downregulated proteins in gallbladder cancer included serine protease HTRA1 and transgelin, which have been reported to be downregulated in several other cancers. PMID: 24657443
22. HIF-2alpha upregulates transgelin indirectly and accumulated TGF-beta1 is a mediator in the upregulation of transgelin by HIF-2alpha under hypoxia. PMID: 24464808
23. In leiomyosarcomas versus all other sarcomas transgelin emerged as the best diagnostic marker PMID: 23174934
24. Expression of SM22 is inhibited in human CRC, and SM22 may act as a positive regulator of the processes of autophagy. PMID: 23538046
25. Our results indicate that transgelin plays a promoting role in tumor progression, and appears to be a novel prognostic marker for advanced pancreatic cancer. PMID: 23331552
26. the expressions of TAGLN were significantly reduced in colorectal carcinoma tissues and cells, and overexpression of TAGLN could decrease the proliferation and invasion and increase the apoptosis of LoVo cells PMID: 23138394
27. COX7A2, TAGLN2 and S100-A10 as novel prognostic markers in Barrett's adenocarcinoma. PMID: 22365974
28. Depletion of SM22 could contribute to tumourigenic properties of cells. Reduction in SM22 would tend to promote cell survival when cells are under hypoxic stress, and may also contribute to increases in actin dynamics that favour metastatic potential. PMID: 22257561
29. Strong transgelin expression is observed in renal tissue of patients with glomerulonephritis; its distribution is comparable to that of alpha-smooth muscle actin (alpha-SMA), a marker of myofibroblast activation in the kidney. PMID: 21677441
30. SM22alpha overexpression enhances tumor cell growth and activates the IGF1R/PI3K/Akt pathway via direct interaction with IGF1Rbeta. PMID: 22245152
31. The expression of Transgelin in the uterine body smooth muscle tissue of pregnant women during labor was higher than in non-labor. PMID: 21051832
32. We found an increased expression of the TAGLN gene in endometriotic lesions compared with the eutopic endometrium of the same patients by real-time polymerase chain reaction. PMID: 21763649
33. Down-regulation of SM22alpha in breast cancer is correlated with lymph node metastasis. PMID: 21092460
34. Results indicate that the metastatic potential of CSCs arises from highly expressed Transgelin. PMID: 20707403
35. these findings provide the first demonstration that SM22alpha modulates cellular senescence caused by damaging agents via regulation of the p16(INK4a)/pRB pathway in HepG2 cells and that these effects of SM22alpha are partially mediated by MT-1G. PMID: 20705054
36. Expression of the actin-associated protein transgelin (SM22) is decreased in prostate cancer PMID: 20012321
37. Loss of SM22 is a molecular signature of colon cancer and is closely associated with progression, differentiation, and metastasis of colon cancer. PMID: 20336793
38. results are indicative of p53-mediated mitochondria-associated apoptotic effects of transgelin on LNCaP cells in addition to its known suppressive effects on the AR pathway PMID: 20098441
39. in pulmonary adenocarcinoma, overexpression of TAGLN was strictly localized to the tumor-induced reactive myofibroblastic stromal tissue compartment, whereas overexpression of TAGLN2 was exclusively localized to the neoplastic glandular compartment PMID: 19848416
40. loss of transgelin gene expression may be an important early event in tumor progression and a diagnostic marker for breast and colon cancer development. PMID: 11773051
41. Transgelin functions as a suppressor to inhibit prostate cancer cell growth PMID: 17082327
42. crystallization and X-ray diffraction of transgelin. PMID: 17305610
43. SM22 expression in SMCs was dramatically higher than in GC cells, which indicates that SM22 is unlikely to be a proper biomarker for GC. PMID: 17629319
44. tagln is a novel target of TGF-beta/Smad3-dependent gene expression in alveolar epithelial type II cells PMID: 18245174
45. 2.3 A resolution crystal structure of full length human transgelin, whose main structural feature is confirmed to be a CH domain. PMID: 18291675
46. acts as a tumour suppressor; expression is lost in prostate, breast and colon cancers PMID: 18378184
47. Identificaton of transgelin a novel biomarker for gastric adenocarcinoma. PMID: 18446369
48. Selective overexpression of airway smooth muscle genes in asthmatic airways leads to increased Vmax, thus contributing to the airway hyperresponsiveness observed in asthma. PMID: 19011151
49. Transgelin was expressed in pulmonary artery smooth myocytes under hypoxia compared with normoxia via and HIF-1alpha-independent pathway. Reducting transgelin expression by RNA interference impaired migration especially under hypoxia. PMID: 19188659
50. Loss of transgelin involves gene promoter hypermethylation and is closely associated with poor overall survival in colorectal cancer patients. PMID: 19329940
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亞細胞定位：

Cytoplasm.
蛋白家族：

Calponin family
數據庫鏈接：

HGNC: 11553

OMIM: 600818

KEGG: hsa:6876

STRING: 9606.ENSP00000278968

UniGene: Hs.410977

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