STAT4 Recombinant Monoclonal Antibody

說明書

中文名稱：

STAT4重組抗體
貨號：

CSB-RA547973A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA547973A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA547973A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of HepG2 cell with CSB-RA547973A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 515-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

STAT4重組單克隆抗體（CSB-RA547973A0HU）是針對信號轉導和轉錄激活因子4（STAT4）開發的高特異性科研工具。STAT4作為JAK-STAT信號通路的關鍵成員，參與調控IL-12和I型干擾素介導的免疫應答，在Th1細胞分化、炎癥反應及自身免疫性疾病機制研究中具有重要價值。本產品采用重組表達技術制備，具備優異的抗原結合能力和批次穩定性，經嚴格驗證可應用于ELISA、免疫組化（IHC）及免疫熒光（IF）實驗，推薦稀釋比例為IHC 1:50-1:200、IF 1:50-1:200。實驗數據顯示，該抗體在多種樣本類型中均能實現特異性識別，可在組織切片中清晰定位STAT4蛋白表達，并在細胞成像實驗中呈現高信噪比的熒光信號。適用于免疫細胞信號轉導機制研究、炎癥性疾病模型中的STAT4表達譜分析，以及自身免疫相關通路的分子互作探索，為免疫學、分子病理學及藥物靶點篩選等基礎研究領域提供可靠工具。
Uniprot No.：

Q14765
基因名：

STAT4
別名：

Signal transducer and activator of transcription 4, STAT4
反應種屬：

Human
免疫原：

A synthesized peptide derived from human STAT4
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

26G2
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Carries out a dual signal transduction and activation of transcription. Involved in IL12 signaling.
基因功能參考文獻：
1. the present study postulates that the genetic variation of the transcription factor GATA3, not STAT4, is associated with the risk of type 2 diabetes in the Bangladeshi population. PMID: 30044774
2. Lack of association of rs7574865 and rs7601754 SNPs in STAT4 gene with susceptibility to JSLE in Iranian population, despite their association with the risk of adult SLE in the same population, implicates on difference of genetic background of JSLE and SLE. PMID: 29276866
3. No significant associations between STAT4 variant and serum neopterin or disease activity parameters were identified. The study confirmed the association of STAT4 rs7574865 polymorphism with rheumatoid arthritis and was the first to indicate an association with rheumatoid factor and anti-cyclic citrullinated peptide antibodies positivity. PMID: 28424905
4. 5 STAT4 SNPs were tested in 233 patients with established NMOSD and 492 healthy controls. Allelic, additive, dominant, and recessive models identified associations with NMOSD. Minor alleles of 4 STAT4 SNPs exhibited significant association with increased risk of NMOSD: rs7574865 T; rs10181656 G; rs10168266 T; and rs13426947 A in all models. rs7601754 G displayed a protective effect against NMOSD. PMID: 28852993
5. data demonstrate that STAT4 plays a role in enabling the normal and timely division of cells undergoing mitosis PMID: 28516569
6. Genome-wide association study identified a locus in STAT4, rs13390936, associated with nontyphoidal Salmonella bacteremia. PMID: 29523850
7. Neither alleles nor genotypes of rs7601754 SNP of STAT4 gene demonstrated associations with juvenile rheumatoid arthritis (JRA). We recognize that gene variants of STAT4 did not affect JRA susceptibility in an Iranian population. PMID: 28524764
8. STAT4 is a novel transcriptional regulator of p66Shc in normal and chronic lymphocytic leukemia B cells PMID: 27494881
9. IL13 AA of rs20541 and STAT4 TT of rs925847 are potential genomic biomarkers for predicting lower pulmonary function. The administration of high-dose ICSs to asthmatic patients with genetic variants of IL13 AA may inhibit the advancement of airway remodelling. The genetic variants of STAT4 TT did not respond to high-dose ICSs. PMID: 26765219
10. STAT4 mRNA expression was significantly correlated with IFNG mRNA expression. PMID: 29187449
11. findings demonstrate that variants in STAT4 play a critical role in HBV infection and clearance in the Chinese Han population. PMID: 27444301
12. STAT4 rs7574865G/T polymorphism is associated with rheumatoid arthritis and systemic lupus erythematosus in Mexican women PMID: 27178308
13. STAT4_rs7574865 TT was associated with the presence of actively inflamed joints and extra-articular damage in patients with Juvenile Idiopathic Arthritis. PMID: 28145159
14. Results found that activated STAT4 was overexpressed in epithelial cells of ovarian cancer and provide evidence that it promotes ovarian cancer metastasis via tumor-derived Wnt7a-induced activation of cancer-associated fibroblasts. PMID: 28114283
15. STAT4 rs7574865 gene polymorphism is associated with the susceptibility of primary biliary cirrhosis in the Han population of Jiangsu province. PMID: 28395724
16. The findings results confirm the important role of STAT4 gene in the predisposition to Systemic sclerosis and its phenotypes, such as the presence of Interstitial Lung disease , cardiac injury , and seropositivity for anti-topoisomerase I antibodies in the Russian population. PMID: 28631694
17. results show specific and dominant contribution of STAT4 in the hematopoietic compartment to metabolic health and inflammation in diet-induced obesity. PMID: 28400678
18. reported that SNPs in STAT4, PTPN2, PSORS1C1, and TRAF3IP2 are associated with response to TNF-i treatment in RA patients; however, these findings should be validated in a larger population PMID: 28107378
19. This is the first demonstration of STAT4 acting as a transcriptional repressor in response to IFN-alpha/beta signaling and highlights the unique activity of this cytokine to acutely block the expression of an inflammatory cytokine in human T cells. PMID: 26990433
20. A higher risk to develop RA was observed for rs7574865 in the STAT-4 gene, while the rs1800872 in the IL-10 gene showed a protective effect. PMID: 27342690
21. STAT4 rs7574865 polymorphism has a role in rheumatoid arthritis and disease activity, but not in anti-CCP antibody levels in a Mexican population PMID: 27234231
22. this study shows that STAT4 single nucleotide polymorphism affects clinical outcomes of pediatric acute leukemia patients after hematopoietic stem cell transplant PMID: 27960128
23. this study shows that STAT4 gene polymorphisms are associated with ankylosing spondylitis in Southwest China PMID: 27394003
24. Our results for the first time showed a significant association between STAT4 rs7582694 alleles and genotypes and susceptibility to endometriosis in a population. PMID: 27235632
25. we can conclude that STAT4-rs7574865 polymorphism is clearly associated with the risk of Rheumatoid Arthritis in the Western Algerian population PMID: 25351936
26. The results demonstrated that STAT4 rs7574865 and IRF5 rs2004640G/T substitution are associated with a susceptibility to systemic sclerosis. PMID: 26712637
27. Our findings indicate that although STAT4 and IFIH1 SNPs are not associated with T1D in a Brazilian population, they might play a role in susceptibility to T1D on a larger worldwide scale PMID: 26782418
28. SNP rs7574865 in STAT4 might contribute to progression to Hepatocellular Carcinoma. PMID: 26745093
29. Five SNPs (rs7574865 in STAT4, rs9267673 near C2, rs2647073 and rs3997872 near HLA-DRB1 and rs9275319 near HLA-DQ), were found to be significantly associated with the risk of HBV-related LC. PMID: 26538132
30. There is a significant association between STAT4 rs7574865 polymorphism and IBD susceptibility in overall population. PMID: 26066297
31. STAT4 protein genetic variation is a prognostic factor predicting the treatment with interferon-alpha therapy in chronic hepatitis B. PMID: 26704347
32. the presence of the rs7574865 T allele enhances STAT4 mRNA transcription and protein expression PMID: 26569609
33. STAT4 rs7574865 polymorphism may be associated with significantly reduced risk of HBV-induced HCC in Asian PMID: 25178516
34. gene polymorphisms along with Ptpn22 polymorphism confers susceptibility to rheumatoid arthritis in all major ethnic groups. [meta-analysis] PMID: 25963842
35. Significant association with alleles of two STAT4 markers and nominal association of Autoimmune Addison's disease with alleles at GATA3, is reported. PMID: 24614117
36. our replication study showed that the rs7574865 in STAT4 and rs9275319 in HLA-DQ were not associated with CHB-related HCC in a Korean population. PMID: 25913043
37. CCR1, KLRC4, IL12A-AS1, STAT4, and ERAP1 are bona fide susceptibility genes for Behcet's disease. PMID: 26097239
38. STAT4 rs7574865 G/T and PTPN22 rs2488457 G/C polymorphisms as susceptibility factors for JIA PMID: 25781893
39. STAT4 minor allele may be associated with the spontaneous clearance of HBV, whereas the major allele may be associated with the progress of the HBV-related liver disease. PMID: 25829184
40. STAT4 may inhibit HCC development by modulating HCC cell proliferation. PMID: 25852285
41. Review/Meta-analysis: STAT4 rs7574865 polymorphism confers susceptibility to rheumatoid arthritis in major ethnic groups. PMID: 24751105
42. Evidence of a higher risk to develop pericarditis with STAT4 genotypes, and an association between HCP5 rs3099844 and anti-Ro/SSA antibodies in Italian systemic lupus erythematosus patients. PMID: 25369137
43. STAT4 rs7574865 seems to be Tibetan specific in hepatitis B virus natural clearance. PMID: 25041342
44. STAT4 rs7574865 seemed not to correlate with hepatitis B virus infection susceptibility or natural clearance; its role in hepatocellular carcinoma development seemed ambiguous PMID: 25365208
45. Loss of STAT4 expression and associated switch to Th2 phenotype during Mycosis Fungoides progression may be driven via aberrant histone acetylation and/or upregulation of oncogenic miR-155 microRNA. PMID: 25486484
46. Increased expression of STAT4 is positively correlated with the depth of invasion in colorectal cancer patients. PMID: 25864744
47. This work reported the association of 14q32.11 (EFCAB11) with Hepatocellular carcinoma in Chinese Han population and revealed the genetic interaction between STAT4 (2q32.2-q32.3) and EFCAB11 (14q32.11) in Hepatocellular carcinoma. PMID: 25665738
48. STAT4 rs7574865/rs10181656 polymorphisms increase the risk of autoimmune thyroid diseases in a Chinese population. PMID: 25019342
49. Rheumatoid arthritis cases showed a significantly higher frequency of the STAT4 T allele carriage (GT+TT genotypes) compared to controls. PMID: 24979672
50. STAT4 plays a crucial role in the function of innate and adaptive immune cells; dysregulated expression and aberrant activation of STAT4 is observed in many human autoimmune conditions. PMID: 24844303
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相關疾病：

Systemic lupus erythematosus 11 (SLEB11); Rheumatoid arthritis (RA)
亞細胞定位：

Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation.
蛋白家族：

Transcription factor STAT family
數據庫鏈接：

HGNC: 11365

OMIM: 180300

KEGG: hsa:6775

STRING: 9606.ENSP00000351255

UniGene: Hs.735572

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