SOS1 Recombinant Monoclonal Antibody

說明書

中文名稱：

SOS1重組抗體
貨號：

CSB-RA561936A0HU
規(guī)格：

￥1320
圖片：
Overlay Peak curve showing Hela cells stained with CSB-RA561936A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA561936A0HU SOS1重組單克隆抗體是經(jīng)過嚴(yán)格驗(yàn)證的高特異性科研工具，靶向Son of Sevenless同源物1（SOS1）蛋白。SOS1作為RAS信號通路的關(guān)鍵調(diào)控因子，通過激活RAS-GTP酶參與細(xì)胞增殖、分化及腫瘤發(fā)生等生物學(xué)過程。本產(chǎn)品經(jīng)ELISA實(shí)驗(yàn)驗(yàn)證具有優(yōu)異的抗原結(jié)合能力，同時(shí)在流式細(xì)胞分析（FC）中展現(xiàn)出良好的細(xì)胞水平檢測性能，推薦使用稀釋比例為1:50-1:200，可有效識別天然構(gòu)象的SOS1蛋白，實(shí)驗(yàn)結(jié)果顯示其具備高信號靈敏度和低交叉反應(yīng)性。該抗體適用于探究RAS/MAPK信號轉(zhuǎn)導(dǎo)機(jī)制、腫瘤細(xì)胞信號異常調(diào)控及藥物靶點(diǎn)篩選等研究領(lǐng)域，可配合體外細(xì)胞模型進(jìn)行功能驗(yàn)證、蛋白互作分析及通路活性檢測。作為科研級試劑，本產(chǎn)品為探索SOS1在疾病相關(guān)分子網(wǎng)絡(luò)中的作用提供了可靠工具，尤其適用于基礎(chǔ)研究與藥物開發(fā)中的靶點(diǎn)表征實(shí)驗(yàn)。
Uniprot No.：

Q07889
基因名：

SOS1
別名：

Son of sevenless homolog 1 (SOS-1), SOS1
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from Human SOS1
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

2B2
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, FC

推薦稀釋比：

Application	Recommended Dilution
FC	1:50-1:200

Protocols：

ELISA Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Promotes the exchange of Ras-bound GDP by GTP. Probably by promoting Ras activation, regulates phosphorylation of MAP kinase MAPK3 in response to EGF. Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity.
基因功能參考文獻(xiàn)：
1. a distinct role of the C-terminal proline-rich (PR) domain of the full-length son of sevenless homolog 1 (SOS1) protein. PMID: 28452363
2. SOS1 was statistically significantly associated with gestational diabetes mellitus risk after adjusting for multiple comparisons PMID: 29233017
3. Findings identified p.Ser548Arg missense mutation in Son of Sevenless Homolog 1 (SOS1) in the boy, confirmed in his mother. PMID: 28456002
4. We report on a novel pathogenic mutation in the SOS1 gene and a large clinical spectrum in a Noonan syndrome family with ten genetically confirmed affected individuals. PMID: 28884940
5. Data indicate dynamic of H-Ras functional cycle as controlled by son of sevenless homolog 1 (Sos). PMID: 27412770
6. In summary, patients from two families with history of non-syndromic autosomal-dominant HGF from Malopolska and Mazovia provinces in Poland had not been affected by HGF type 1, caused by a single-cytosine insertion in exon 21 of the SOS-1 gene. PMID: 28425619
7. Mutation scanning of the entire coding sequence of SOS1 gene identified seven intronic variants and one new exonic substitution (c.138G > A). The seven common intronic variants were not considered to be of pathogenic importance. The exonic substitution c.138G > A was found to be absent in 100 ethnically matched normal control chromosomes, not expected to have functional significance based on prediction bioinformatics tool PMID: 28433619
8. Grb2-independent interactions are sufficient to retain human SOS on the membrane for many minutes, during which a single SOS molecule could processively activate thousands of Ras molecules. PMID: 27501536
9. a computational methodology that overlays any variant database onto the somatic mutations in all cancer exomes identified activating SOS1 mutations associated with Noonan syndrome as significantly altered in melanoma PMID: 27304678
10. Data show that a heterozygous son of sevenless homolog 1 (SOS1) gene frameshift mutation (c.3266dup or c.3248dup) was identified in each patient. PMID: 26708403
11. Ras.GDP weakly binds to the catalytic but not to the allosteric site of Sos. PMID: 26565026
12. The present study provides a first evidence of allelic imbalance of SOS1 and pinpoints this condition as a possible mechanism underlying a different penetrance of some SOS1-mutated alleles in unrelated carriers PMID: 25712082
13. In non-apoptotic cells, nuclear EGFR induced SOS1 expression by directly binding to the SOS1 promoter. PMID: 25980493
14. rs963731 is associated with corticobasal degeneration. PMID: 26077951
15. findings suggest that targeting the Src/Abl/Sos1/Rac pathway may represent a double-edged sword to control both cancer-invasive capacities and cancer-related inflammation. PMID: 26447228
16. These data demonstrated the negative regulation between miR-146a and SOS1 and between miR-370 and GADD45beta and that these regulations are influenced by enterovirus 71 to induce apoptosis. PMID: 25469565
17. Combined rational design and a high throughput screening platform for identifying chemical inhibitors of a Ras-activating enzyme. PMID: 25825487
18. SOS1 and Ras regulate epithelial tight junction formation in the human airway through EMP1. PMID: 25394671
19. HGF-related mutation g.126,142-126,143insC in exon 21 was not found in any of the 6 affected individuals from three families. PMID: 25062969
20. Stabilized alpha helices of son of sevenless 1 directly inhibit wild-type and mutant forms of KRAS. PMID: 25624485
21. CIIA functions as a negative modulator of the SOS1-Ras signaling events initiated by peptide growth factors including EGF. PMID: 24522193
22. Increased expression of SOS1 increases NFkappaB activation in several types of cancer cells, and ablation of SOS1 inhibits EGF-induced NFkappaB activation in these cells, indicating that SOS1 is a component of the pathway connecting EGFR to NFkappaB activation. PMID: 25071181
23. sustained Erk signaling and T-cell activation depend on both Sos1 and RasGRP1. PMID: 24497027
24. MiR-124 inhibits the growth of glioblastoma through the downregulation of SOS1. PMID: 23817964
25. Although Sos1 and Gab1 recognize two non-overlapping sites within the Grb2 adaptor, allostery promotes the formation of two distinct pools of Grb2-Sos1 and Grb2-Gab1 binary signaling complexes in lieu of a composite Sos1-Grb2-Gab1 ternary complex. PMID: 23334917
26. The SOS1 T158A mutation altered the phosphorylation of gene products involved in both RAS/MAPK and PI3K/AKT pathways. PMID: 23528009
27. The PR domain displays a highly dynamic conformational basin in agreement with the knowledge that the intrinsically unstructured proteins rapidly interconvert between an ensemble of conformations. PMID: 23528987
28. study established that the presence of SOS1, but not its enzymatic activity, is critical for p38 activation PMID: 23589333
29. Oncogenic K-Ras promotes the activation of wild-type H- and N-Ras and this activation is mediated by oncogenic K-Ras-dependent allosteric stimulation of Sos. PMID: 23132018
30. T-cell antigen receptor (TCR)-mediated Erk activation requires RasGRP1, but not Grb2/Sos. PMID: 22344067
31. Modeling and simulation of aggregation of membrane protein LAT with molecular variability in the number of binding sites for cytosolic Grb2-SOS1-Grb2 PMID: 22396725
32. We present a stochastic mathematical model describing intra-molecular regulation of hSos1 activity. PMID: 21851854
33. multiple binding sites within Sos1 provide a physical route for Grb2 to hop in a flip-flop manner from one site to the next through facilitated diffusion, and such rapid exchange forms the basis of cooperativity driving bivalent binding of Grb2 to Sos1 PMID: 22360309
34. SOS1 over-expression may play a role in the regulation of the RAS/mitogen-activated protein kinase pathway in the skin, in the hair follicle proliferation and cell cycle, suggesting new perspectives in understanding the pathogenesis of hirsutism. PMID: 22217993
35. CIIA functions as a molecular switch for the GEF activity of SOS1, directing this activity toward Rac1. PMID: 22042618
36. The researchers found evidence that there were significant differences between the D2S441 locus in the Maghreb population and other populations. PMID: 21674833
37. this report expanded the available information about the molecular diversity of SOS1 mutations underlying Noonan syndrome, and have provided a more comprehensive assessment of the clinical features associated with those molecular lesions. PMID: 21387466
38. Comparison of RasGEF expression at different developmental stages showed that relative to Sos2 and RasGRP1, Sos1 is most abundant in DN thymocytes, but least abundant in DP thymocytes. PMID: 21746917
39. two unrelated patients with Noonan syndrome carrying the same heterozygous SOS1 missense mutation (c.1867T > A/p.F623I) PMID: 20673819
40. A case mimicking CS with SOS1 T158A substitution, which has not been reported previously in CS, revealed the complex relationship between the genotype and phenotype of overlapping syndromes of the RAS/RAF/MEK/ERK pathway. PMID: 20030748
41. This study investigated the regulation of the previously uncharacterized SOS1 gene promoter by the aryl hydrocarbon receptor and its ligands in HepG2 cells. PMID: 20950586
42. mutation analysis performed on RAF1, SOS1, and GRB2, in 24 Noonan syndrome patients previously found to be negative for PTPN11 and KRAS mutations; SOS1 may have a role of modifier gene that might contribute the variable expressivity of the disease PMID: 20683980
43. Noonan syndrome is due to a SOS1 missense mutation and rhabdomyosarcoma. PMID: 20607846
44. Multiple decisive phosphorylation sites for the negative feedback regulation of SOS1 via ERK. PMID: 20724475
45. Noonan-like/multiple giant cell lesion syndrome with mutations in the SOS1 gene PMID: 20305546
46. Based on our results, it is possible that a subtle dysfunction (expression) of the SOS1 gene is involved in the development of the most common male reproductive tract disorder - unilateral or bilateral cryptorchidism PMID: 20389169
47. a new crystal structure of SOS at 3.2 A resolution was presented that contains the histone domain and the DH-PH unit in addition to the catalytic segment (SOSHDPC, residues 1-1049). PMID: 20133692
48. Sos-histone domain plays a critical role in governing the catalytic output of Sos through the coupling of membrane recruitment to the release of autoinhibition. PMID: 20133694
49. First report describing different tumor types in Noonan syndrome with germline SOS1 mutations. PMID: 19953625
50. The binding of Grb2 adaptor to its downstream partners Sos1 and Gab1 docker is under tight allosteric regulation. PMID: 20005866
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相關(guān)疾病：

Fibromatosis, gingival, 1 (GINGF1); Noonan syndrome 4 (NS4)
組織特異性：

Expressed in gingival tissues.
數(shù)據(jù)庫鏈接：

HGNC: 11187

OMIM: 135300

KEGG: hsa:6654

STRING: 9606.ENSP00000384675

UniGene: Hs.709893

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