SAA1 Recombinant Monoclonal Antibody

說明書

中文名稱：

SAA1重組抗體
貨號：

CSB-RA123000A0HU
規格：

￥1320
圖片：
Overlay Peak curve showing HepG2 cells stained with CSB-RA123000A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA123000A0HU SAA1重組單克隆抗體是針對人血清淀粉樣蛋白A1（SAA1）研發的高特異性檢測工具。SAA1是一種急性期反應蛋白，主要由肝臟合成并在炎癥、感染或組織損傷時顯著上調，其參與調控先天免疫應答、膽固醇代謝及細胞信號轉導等過程，與動脈粥樣硬化、類風濕性關節炎及腫瘤微環境調控等病理生理機制密切相關。本抗體經ELISA和流式細胞術（FC）嚴格驗證，在FC應用中推薦使用1:50至1:200稀釋比例，可實現對內源性SAA1蛋白的高靈敏度檢測，其特異性經交叉反應實驗確認，適用于細胞表面或胞內靶標的高分辨率分析。該產品可為炎癥相關疾病模型構建、免疫細胞激活狀態評估、代謝調控通路研究提供可靠工具，尤其適用于體外培養細胞、原代免疫細胞或血清樣本中SAA1表達水平的動態監測及功能機制解析，滿足免疫學、細胞生物學及分子病理學領域的科研需求。
Uniprot No.：

P0DJI8
基因名：

SAA1
別名：

Serum amyloid A-1 protein (SAA) [Cleaved into: Amyloid protein A (Amyloid fibril protein AA), Serum amyloid protein A(2-104), Serum amyloid protein A(3-104), Serum amyloid protein A(2-103), Serum amyloid protein A(2-102), Serum amyloid protein A(4-101)], SAA1
反應種屬：

Human
免疫原：

A synthesized peptide derived from Human SAA1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

20G5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, FC

推薦稀釋比：

Application	Recommended Dilution
FC	1:50-1:200

Protocols：

ELISA Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Major acute phase protein.
基因功能參考文獻：
1. SAA1 can be produced in human fetal membranes, which can be greatly induced in the presence of proinflammatory cytokines and glucocorticoids thereby producing effects associated with parturition PMID: 28386088
2. High SAA1 expression in the stromal component is associated with pancreatic tumors. PMID: 29351990
3. Data suggest that anti-inflammatory capacity of HDL (high-density lipoprotein) from patients with type 2 diabetes and diabetic nephropathy is impaired; this effect might be attributable to SAA enrichment in HDL in these patients; up-regulation of SAA in HDL appears to be associated with increased risk of diabetic angiopathy or vasculitis in such patients. PMID: 28760652
4. The frequencies of the CC genotype and the C allele of SAA rs12218 were higher in participants with ischemic stroke than in the control group. The frequencies of the AG genotype and the G allele of rs2468844 were higher in participants with ischemic stroke than in the control group. Multiple logistic regression analysis revealed the significance of the rs12218 in males and in large-artery atherosclerosis group. PMID: 28870546
5. we show that astrocytoma patients have increased levels of serum SAA and SAA1 is expressed and secreted in glioblastoma, and its co-expression with tumor-related genes supports its involvement in glioblastoma angiogenesis and progression. PMID: 28283801
6. These results suggested that the C-terminal truncation of human SAA accelerates amyloid fibril formation. PMID: 29288051
7. SAA1 can be a potential mediator for UV-induced MMP-1 expression in human skin. PMID: 26900010
8. Data suggest that serum amyloid A (SAA) immunoreactivity in tumor-associated macrophage (TAM) is associated with worse recurrence-free survival, and is perhaps a therapeutic target for breast cancer. PMID: 27058895
9. Findings indicate that in Lyme disease patients, circulating CRP and SAA (Serum Amyloid A) levels are highest when the concentration of spirochetes is greatest in skin and/or blood and that levels decline after the dissemination of the organism to extracutaneous sites in subsequent stages of infection. PMID: 27585799
10. The Serum amyloid A (SAA)/formyl peptide receptor-like 1 (FPRL1) contributed to pathogenesis of psoriasis by promoting keratinocyte proliferation and inflammation, thus providing a potential therapeutic target for disease therapy. PMID: 27910163
11. High SAA expression is associated with lung cancer. PMID: 27809798
12. SAA, PROZ, and C4BPB may serve as new potential biomarkers for TB PMID: 28278182
13. Novel truncated form of serum amyloid A is elevated in the plasma of Kawasaki disease (KD) when compared with Febrile control subjects. Future studies will evaluate its relevance as a diagnostic biomarker and its potential role in the pathophysiology of KD. PMID: 27271757
14. SR-A1 suppresses lung cancer metastasis by downregulating SAA1 production in tumor-associated macrophages (TAM). PMID: 28202524
15. The SAA1.1 allele was found in four familial Mediterranean fever patients, including two with AA amyloidosis. PMID: 27150194
16. genetic variants at the CRP and SAA1 loci independently affect both CRP and SAA levels, and their respective circulating levels act as suppressors. PMID: 27313400
17. Serum amyloid A could represent a novel marker of primary unexplained recurrent early pregnancy loss because affected women exhibited elevated levels of this protein. PMID: 28099717
18. The polymorphism of SAA1 is not associated with susceptibility and severity of Familial Mediterranean Fever in Egyptian children PMID: 27300189
19. The findings identify SR-BII as a functional SAA receptor that mediates SAA uptake and contributes to its proinflammatory signaling via the MAPK-mediated signaling pathways. PMID: 28423002
20. Correlate MEFV genotype and the SAA1 polymorphisms with the clinical manifestations of familial Mediterranean fever and the occurrence of amyloidosis in a large cohort of Armenian patients. PMID: 27791951
21. Study found increased SAA concentration in patients with sarcoidosis. PMID: 26919159
22. the detection of residues 76 and 77 of SAA (AA76) may alter the ability to diagnose AA amyloidosis. PMID: 27098620
23. The results demonstrated that SAA upregulated Visfatin expression in cultured RAW264.7 macrophages and in the primary monocytes. PMID: 27006946
24. Structure, function and SAA1 gene polymorphisms PMID: 26945629
25. Serum Amyloid A induces inflammation, proliferation and cell death in activated hepatic stellate cells. PMID: 26937641
26. Amyloid A overexpressed in renal cell carcinoma patients and can serve as a prognostic marker. PMID: 26750935
27. incorporation of SAA into HDL preparations reduced antiinflammatory properties but not to the same extent as HDL from AgNO3-injected mice PMID: 26642365
28. human SAA is possibly only expressed in a subset of septic patients; SAA induces HMGB1 release via TLR4 and RAGE receptors; SAA supplementation worsens the outcome of lethal endotoxemia PMID: 26052716
29. The SAA1 rs12218 polymorphism was significantly more prevalent in ankylosing spondylitis patients with amyloidosis. PMID: 26300108
30. Autocrine/paracrine and recombinant human SAA1 via TLR4 stimulate a proinflammatory phenotype that is both part of the early phase of osteogenic differentiation and the development of senescence. PMID: 26135899
31. Serum amyloid A1alpha induces paracrine IL-8/CXCL8 via TLR2 and directly synergizes with this chemokine via CXCR2 and formyl peptide receptor 2 to recruit neutrophils. PMID: 26297794
32. These functional properties set SAA apart from well-characterized inflammatory factors, such as LPS and TNF-alpha, suggesting that it may play a homeostatic role during the course of inflammation. PMID: 26130702
33. The detailed analysis of the docking results points to the fact that the best serum amyloid A-Cystatin C (SAA-hCC) binding is achieved by the peptides inclined toward interacting via Lys90 and Arg 96 (with ffhCC Ser98 and Tyr102, respectively). PMID: 25736604
34. Our results showed PCT as a valuable marker of bacterial infections in febrile patients. PCT was superior to CRP, IL-6 or SAA in the early identification of bacterial infection PMID: 25963492
35. Mutations in genetic biomarker SAA1 gene related to amyloidosis processes may play a crucial role in chronic renal failure patients who require long-term hemodialysis. PMID: 25394530
36. The relative abundance of the N-terminal arginine truncation of SAA1.1 is significantly decreased in diabetes and negatively correlates with measures of glycemic and lipid control. PMID: 25607823
37. potential marker for detecting the activity of srcoidosis PMID: 25623898
38. Genetic polymorphisms in SAA1 are associated with coronary artery disease in the Han and Uygur populations in Western China. PMID: 25656165
39. High SAA serum level is associated with hepatocellular carcinoma. PMID: 25605163
40. HDL3 subpopulations in ST segment elevation myocardial infarction were enriched 10 times in SAA patients. PMID: 26037829
41. SAA has a role in activity of rheumatoid arthritis and risk of cardiovascular and renal involvement PMID: 25525305
42. SR-B1 and p38 MAPK are involved in signaling pathway of serum amyloid A-induced angiogenesis in rheumatoid arthritis. PMID: 25932604
43. Although SAA concentrations were elevated in the sera, there was no significant difference in these concentrations between familial Mediterranean fever patients and rheumatoid arthritis patients. PMID: 25240611
44. Zn deficiency enhances the acute phase response and particularly the JAK-STAT3 pathway, resulting in increased serum amyloid A production. PMID: 24732911
45. Serum amyloid A is a retinol binding protein that transports retinol during bacterial infection. PMID: 25073702
46. Data suggest that serum amyloid A (SAA) is a candidate for diabetic kidney disease therapeutic and biomarker discovery. PMID: 25531567
47. The CSF biomarker amyloid 1-42 is important and useful as part of the diagnostic procedure for detecting AD and other dementia in elderly patients displaying psychotic symptoms. PMID: 23597931
48. homozygous SAA1.5/1.5 genotype appears to be a recessive susceptibility gene, which has lost the antiangiogenic function, whereas SAA1.1 and SAA1.3 are the dominant alleles of the tumor suppressor phenotype. PMID: 24608426
49. keratinocyte-derived SAA triggers a key inflammatory mediator, IL-1beta, via NLRP3 inflammasome activation, providing new potential targets for the treatment of this chronic skin disease. PMID: 25231464
50. SAA1 gene expression in COPD patients is responsible for the secretion of this molecule in different cell types. PMID: 24884805
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相關疾病：

Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA1 protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
亞細胞定位：

Secreted.
蛋白家族：

SAA family
組織特異性：

Expressed by the liver; secreted in plasma (at protein level).
數據庫鏈接：

HGNC: 10513

OMIM: 104750

KEGG: hsa:6288

STRING: 9606.ENSP00000348918

UniGene: Hs.632144

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