RDX Recombinant Monoclonal Antibody

說明書

中文名稱：

RDX Recombinant Monoclonal Antibody
貨號：

CSB-RA098472A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: HEK293 whole cell lysate(30μg),HT-29 whole cell lysate(30μg),Hela whole cell lysate(30μg),k562 whole cell lysate(30μg),U-251MG whole cell lysate(30μg),NIH/3T3 whole cell lysate(30μg)
All lanes: Radixin antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 69 kDa
Observed band size: 69,80 kDa
Exposure time：2min

Overlay Peak curve showing HepG2 cells stained with CSB-RA098472A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

P35241
基因名：

RDX
別名：

CB567 antibody; CG12537 antibody; DFNB24 antibody; ESP10 antibody; Hh-induced MATH and BTB domain-containing protein antibody; HIB antibody; Moesin-B antibody; Protein roadkill antibody; RADI_HUMAN antibody; Radixin antibody; RDX antibody
反應種屬：

Human, Mouse
免疫原：

A synthesized peptide derived from human RDX
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

15A6
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
基因功能參考文獻：
1. CPI-17 drives Ras activity and tumorigenesis in melanomas in a two-fold way; inactivation of the tumor suppressor merlin and activation of the growth promoting ERM family. PMID: 27793041
2. The results reveal a supportive role of ERMs in cortical activities during cytokinesis, and also provide insight into the selective mechanism that preferentially associates cytokinesis-relevant proteins with the division site. PMID: 28889652
3. Radixin knockdown suppresses the metastasis of SGC-7901 cells in vitro by up-regulation of E-cadherin. The NF-kappaB/snail pathway contributes to the regulation of E-cadherin in response to depletion of radixin. PMID: 27855404
4. Phospho-Ezrin/Radixin/Moesin (ERM) inhibit cell adhesion, and therefore, dephosphorylation of ERM proteins is essential for cell adhesion.Phospho-ERM induce formation and/or maintenance of spherical cell shape. PMID: 26555866
5. These studies identify Akt2 as a critical kinase that regulates radixin phosphorylation and leads to Mrp-2 translocation and function. PMID: 26500117
6. Intracellular sphingosine kinase 2-derived sphingosine-1-phosphate mediates epidermal growth factor-induced ezrin-radixin-moesin phosphorylation and cancer cell invasion. PMID: 26209696
7. High Radixin expression is associated with glioblastoma. PMID: 25520109
8. Ezrin, radixin and moesin are unlikely targets for autoantibodies in demyelinating neuropathies. PMID: 25286001
9. These data suggest an association between RDX polymorphisms and the clinical features of RA patients, particularly the ESR PMID: 24854655
10. Radixin was identified as a target gene of miR-196a/-196b. Elevated miR-196a/-196b expression in GC cells led to reduced radixin protein levels and vice versa. PMID: 24933454
11. This study finding have implications concerning the importance of concomitant radixin upregulation in tumor progression and poor prognosis of patients with gliomas. PMID: 24380686
12. important role of ezrin-moesin-radixin proteins during HCV infection at the postentry level and highlight possible novel targets for HCV treatment. PMID: 23703860
13. ERM proteins play important differential roles in the thrombin-induced modulation of EC permeability, with moesin promoting barrier dysfunction and radixin opposing it. PMID: 23729486
14. Data suggest that P-glycoprotein associate with the F-actin cytoskeleton through ezrin/radixin/moesin (ERM) in CCR9/CCL25 induced multidrug resistance of acute T-lymphocytic leukemia (T-ALL) cells. PMID: 23326330
15. Control of adipogenesis by ezrin, radixin and moesin-dependent biomechanics remodeling. PMID: 23116763
16. Data show that silencing of radixin (RDX) phenocopied the effects of miR-409 overexpression, whereas restoration of RDX in miR-409-overexpressed gastric cancer (GC) cells reversed the suppressive effects of miR-409. PMID: 22179828
17. Ezrin/radixin/moesin are required for the purinergic P2X7 receptor (P2X7R)-dependent processing of the amyloid precursor protein. PMID: 22891241
18. results suggest that radixin might play a critical role in pancreatic cancer progression, possibly through involvement of down-regulation of TSP-1 and E-cadherin expression. PMID: 22631643
19. role of CCM3 and ezrin/radixin/moesin family of proteins in cell's response to oxidative stress PMID: 22291017
20. Ano1, ezrin, and moesin/radixin colocalize apically in salivary gland epithelial cells, and overexpression of moesin and Ano1 in HEK cells alters the subcellular localization of both proteins PMID: 22685202
21. Radixin selectively modulates the expression and function of MRP2 in human gastric adenocarcinoma. PMID: 22469515
22. MiR-31 targeted radixin predominantly via inhibition of protein translation instead of degradation of mRNA. PMID: 22089331
23. results indicate that ezrin influences the expression of P-gp at the translational level, whereas radixin is involved in membrane localization of P-gp in HepG2 cells PMID: 21837648
24. The ERM (ezrin, radixin, moesin) proteins are novel scaffolds at the level of SOS activity control, which is relevant for both normal Ras function and dysfunction known to occur in several human cancers. PMID: 22132106
25. Ezrin, radixin, and moesin are phosphorylated in response to 2-methoxyestradiol and modulate endothelial hyperpermeability. PMID: 21659656
26. the ezrin, radixin and moesin proteins differentially modulate sphingosine 1 phosphate-induced alterations in lung endoethlial cell cytoskeleton and permeability PMID: 21864676
27. This study provides the novel evidence that increased phosphorylation of Ezrin/radixin/moesin proteins may contribute to proliferation of rheumatoid fibroblast-like synoviocytes. PMID: 21278069
28. Dysregulation of the ezrin/radixin/moesin-RAGE complex might be an important step in rearrangement of the actin cytoskeleton during proinflammatory cytokine-induced epithelial-mesenchymal transition of human alveolar epithelial cells. PMID: 21278261
29. ezrin, radixin and moesin play similar roles in the tumor cell metastatic potential and their roles of upregulating the expression of E-cadherin may be important in tumor progression PMID: 21352885
30. Polar distribution of radixin suggest a role in promoting formation and plasticity of membrane surface projections and also suggest that radixin is an organizer and regulator of Mrp-2 and cell polarity in hepatocytes. PMID: 21160029
31. the identification of radixin as a scaffolding unit for both cAMP effectors, Epac and PKA PMID: 21047789
32. Results demonstrate a new regulatory mechanism of ezrin-radixin-moesin phosphorylation by sphingolipids with opposing actions of ceramide and sphingosine 1-phosphate. PMID: 20679347
33. Data suggest that ezrin-radixin-moesin proteins are involved in the spatial regulation of Epac1 and cooperate with cAMP- and Rap-mediated signaling to regulate adhesion to the extracellular matrix. PMID: 20855527
34. amino-terminal domains of the ezrin, radixin, and moesin (ERM) proteins bind advanced glycation end products and have a role in diabetes PMID: 12734202
35. Disrupted localization of radixin and MRP2 supports the concept that radixin contributes to the canalicular localization of MRP2. PMID: 14568249
36. ezrin/radixin/moesin proteins are recruited by NHE1 Na+/H+ exchanger and have roles in regulating Akt-dependent cell survival PMID: 15096511
37. All three ERM family members can localise to the nucleus; a specific nuclear localisation sequence, which is conserved and functional in all ERM family members, is identified, implying specific regulated nuclear import. PMID: 15149851
38. results suggest that ezrin-radixin-moesin proteins are required for microvillar positioning of L-selectin and that this is important both for leukocyte tethering and L-selectin shedding PMID: 15178693
39. Ezrin, radixin and moesin are ADP-ribosylated by Pseudomonas aeruginosa ExoS PMID: 15252013
40. Conformational regulation of radixin protein function occurs by association of the FERM and C-terminal domains, whereby the membrane- and actin-binding activities are mutually suppressed [review] PMID: 15313460
41. Expression of ExoS in HeLa cells led to a loss of phosphorylation of Ezrin/radixin/moesin proteins that was dependent upon the expression of ADP-ribosyltransferase activity. PMID: 16889625
42. 2 mutant alleles of RDX in 2 consanguineous families associated with neurosensory hearing loss; sequence analysis of RDX from original DFNB24 family revealed a c.463C>T transition substitution predicted to truncate radixin in the FERM domain PMID: 17226784
43. increased CD44, ezrin, radixin, and moesin phosphorylation represents a key molecular abnormality that guides T cell adhesion and migration in SLE patients. PMID: 17237445
44. RDX was differentially expressed in sclerotic hippocampi compared to non-sclerotic ones. PMID: 17515952
45. Report disturbed colocalization of multidrug resistance protein 2 and radixin in human cholestatic liver diseases. PMID: 17725603
46. Radixin and ezrin play similar roles in the apical membrane localization of ABCC2 (MRP2) and their expression level and subcellular distribution are important factors in the regulation of ABCC2 (MRP2) at the post-transcriptional level. PMID: 17825285
47. the ezrin, radixin, and moesin proteins function as positive regulators of infection by X4-tropic HIV-1 PMID: 18295815
48. Unlike ezrin and moesin, radixin (a member of the ezrin-radixin-moesin protein family)is not expressed in Jurkat cells, nor is it involved in apoptotic signaling following Fas receptor triggering in these cells. PMID: 18941185
49. No aspects of T cell receptor signaling uniquely require transgenic ezrin or moesin; instead, T cell activation appears to depend on net ezrin, radixin, and moesin (ERM)protein expression, while ezrin and moesin function together. PMID: 19124745
50. In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin PMID: 19129194
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相關疾病：

Deafness, autosomal recessive, 24 (DFNB24)
亞細胞定位：

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cell projection, microvillus. Note=Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.
數據庫鏈接：

HGNC: 9944

OMIM: 179410

KEGG: hsa:5962

STRING: 9606.ENSP00000342830

UniGene: Hs.263671

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