RAN Recombinant Monoclonal Antibody

說明書

中文名稱：

RAN重組抗體
貨號：

CSB-RA191819A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA191819A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.78% DAB.

Immunofluorescence staining of Hela with CSB-RA191819A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 527-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing Hela cells stained with CSB-RA191819A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA191819A0HU RAN重組單克隆抗體是針對RAS相關核蛋白（RAN）研發的高特異性檢測工具。RAN作為RAS超家族成員，是調控核質運輸的關鍵小GTP酶，參與維持核膜完整性、有絲分裂紡錘體組裝及mRNA出核等核心生物學過程。本抗體經多種技術平臺驗證，在ELISA、免疫組化（IHC）、免疫熒光（IF）和流式細胞術（FC）中均表現出優異性能，推薦使用濃度分別為IHC 1:50-1:200、IF 1:50-1:200、FC 1:50-1:200，可精準識別內源性RAN蛋白。實驗數據顯示其具有低交叉反應性和高批次間穩定性，適用于檢測多種哺乳動物樣本中RAN的亞細胞定位及表達水平變化。該產品主要服務于細胞周期調控、核轉運機制、腫瘤微環境等基礎研究領域，特別在探究癌癥細胞異常增殖、耐藥性形成及相關信號通路研究中具有重要應用價值，為科研人員提供可靠的分子檢測工具。
Uniprot No.：

P62826
基因名：

RAN
別名：

GTP-binding nuclear protein Ran (Androgen receptor-associated protein 24) (GTPase Ran) (Ras-like protein TC4) (Ras-related nuclear protein), RAN, ARA24
反應種屬：

Human
免疫原：

A synthesized peptide derived from Human RAN
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

15A8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation. Required for normal progress through mitosis. The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
基因功能參考文獻：
1. DICER rs3742330 AG+GG genotype was associated with more advanced T stage compared to AA genotype ( P=0.009). More patients with XPO5 rs2257082 CC genotype had poorly differentiated tumors compared with CT+TT genotype carriers..), carriers of RAN rs14035 CC genotype had higher three-year OS rate than carriers of CT+TT genotype (adjusted HR 3.174; 95% CI 1.010, 9.973; P=0.048). PMID: 29683064
2. Taken together, the results provide strong in vitro evidence of the involvement of Ran in the progression of breast cancer and suggest that it could have high potential as a therapeutic target and/or marker of disease. PMID: 29750309
3. Molecular structure of the exportin Xpo4 in complex with RanGTP and the hypusine-containing translation factor eIF5A has been reported. PMID: 27306458
4. The mobility of Emerin depends on RanGTP. In cells overexpressing YFP-Emerin, the mobility of YFP-Emerin was higher in Samp1 knock out cells and lower in cells overexpressing Samp1, suggesting that Samp1 significantly attenuates the mobility of Emerin in the nuclear envelope.The affinity between Samp1 and Emerin is decreased in the presence of Ran, suggesting that Ran attenuates the interaction between Samp1 and Emerin. PMID: 29510091
5. KPNB1 and Ran protein jointly mediated the nuclear import of NDV M protein, showing that KPNB1 protein interacted with NDV M protein to form binary complex and then entered into the nucleus with the assistance of Ran protein. PMID: 29746765
6. Ran GTPase promotes cancer progression via Met recepto-rmediated downstream signaling PMID: 27716616
7. Data show that the distribution of components of the ras-related nuclear protein (Ran) pathway that influence microtubule behaviors is determined by their interactions with microtubules, resulting in microtubule nucleators being localized by the microtubules whose formation they stimulate. PMID: 27439876
8. The RAS-related nuclear protein ((P) ran), breast cancer metastasis suppressor 1 ((P) brms1) and minichromosome maintenance complex component 5 ((P) mcm5) promoters have the specificity and strength needed for cancer-specific expression-targeted gene therapy. PMID: 27140445
9. Authors found that decreasing Ran-GTP levels or tethering active Ran to the equatorial membrane affects anillin's localization and causes cytokinesis phenotypes. PMID: 28931593
10. lysine-acetylation regulates nearly all aspects of Ran-function such as RCC1 catalyzed nucleotide exchange, intrinsic nucleotide hydrolysis, its interaction with NTF2 and the formation of import- and export-complexes. PMID: 26507377
11. These results showed that BLM enters the nucleus via the importin beta1, RanGDP and NTF2 dependent pathway, demonstrating for the first time the nuclear trafficking mechanism of a DNA helicase. PMID: 29017749
12. RAN Translation Regulated by Muscleblind Proteins in Myotonic Dystrophy Type 2 PMID: 28910618
13. we demonstrated that DICER (rs3742330) and RAN (rs14035) were associated with the survival of HCC patients PMID: 27611467
14. RAN translation from antisense CCG repeats generates novel proteins that accumulate in ubiquitinated inclusions in Fragile X-associated tremor/ataxia syndrome patients. PMID: 27761921
15. interaction of Samp1 and Ran in the inner nuclear membrane PMID: 27541860
16. RAN role in cell cycle, DNA repair and DNA damage-induced cell senescence PMID: 26864624
17. Binding of Ran to NTF2 is required for NTF2 to inhibit nuclear expansion and import of large cargo molecules. PMID: 26823604
18. RAN is pivotal in linking spatiotemporal control of centrosome regulators to the cell cycle machinery. PMID: 26725228
19. FGF2 nuclear translocation is regulated by Karyopherin-beta2 and Ran GTPase in human glioblastoma cells PMID: 26056081
20. Enterovirus 71-induced downregulation of miR-197 expression increased the expression of RAN, which supported the nuclear transport of the essential viral proteins 3D/3CD and host protein hnRNP K for viral replication. PMID: 26581983
21. Inter-cellular transport of ran GTPase PMID: 25894517
22. Variations in RAN rs14035of Korean patients are significantly associated with their risk of colorectal cancer. PMID: 26147304
23. Data show that RNA-binding protein LIN28B coordinates the expression of the oncogene RAN protein and aurora A kinase (AURKA) in neuroblastoma. PMID: 26481147
24. Elevated metaphase RanGTP levels use Ubr5 to couple overall chromosome congression to SAC silencing. PMID: 26438829
25. Data show that importin-beta (impbeta) alters the nuclear pore's permeability in a Ran-dependent manner, suggesting that impbeta is a functional component of the nuclear pore complex (NPC). PMID: 25748139
26. RAN nucleo-cytoplasmic transport and mitotic spindle assembly partners XPO7 and TPX2 have roles in serous epithelial ovarian cancer PMID: 24625450
27. comparison of the GTPase reaction of the slower hydrolyzing GTPase Ran with Ras PMID: 26272610
28. Ran silencing did not affect the A375 invasive capability, while it was enhanced in 526 cells, suggesting that Ran knockdown, by AurkA down-regulation, resulted in a Ran-independent enhanced melanoma cell invasion. PMID: 25444926
29. stress can disrupt the Ran gradients through RCC1-dependent and RCC1-independent mechanisms PMID: 25452301
30. based on the new structures suggests LM phosphorylation status may mediate Ran's selection of exportin(s) and cargo(s), perverting these native trafficking elements into the lethal antihost Nup phosphorylation pathways. PMID: 25331866
31. PI3Kbeta regulates the nuclear envelope through upstream regulation of RCC1 and Ran PMID: 25348717
32. Towards understanding its molecular mechanism, a 2.9 A resolution crystal structure of human TRN-SR2 complexed with the small GTPase Ran has been determined PMID: 24915079
33. Association between recurrent pregnancy loss development and the polymorphism of the miRNA machinery gene RAN and combined genotype of DROSHA/DICER. PMID: 24769857
34. reducing the nuclear concentration of Ran is sufficient to induce reactive oxygen species irrespective of progerin. PMID: 24523287
35. Our findings emphasize the important role of Ran in differentiation, disease stage, and metastasis in human colorectal cancer PMID: 22956174
36. our data suggest a model for mitotic spindle positioning in which RanGTP and CLASP1 cooperate to align the spindle along the long axis of the dividing cell. PMID: 23783028
37. Thioredoxin-like protein 2 is overexpressed in colon cancer and promotes cancer cell metastasis by interaction with ran. PMID: 23311631
38. MiR-203 may act as novel tumor suppressor in esophageal cancer through down-regulating the expression of Ran and miR-21. PMID: 24001611
39. Polymorphism in RAN gene is associated with HBV-related hepatocellular carcinoma PMID: 23868705
40. a model wherein one monomer of TRN-SR2 is bound to one monomer of RanGTP. PMID: 23878195
41. Overexpressed Ran in pancreatic cancer tissues was found highly correlated with the histological grade. Downregulation of Ran led to significant suppression of cell proliferation, cell cycle arrest at the G1/S phase and induction of apoptosis. PMID: 24076388
42. Data show that five genes CKAP5, KPNB1, RAN, TPX2 and KIF11 were shown to be essential for tumor cell survival in both head and neck squamous cell carcinoma (HNSCC)and non-small cell lung cancer (NSCLC), but most particularly in HNSCC. PMID: 23444224
43. Therefore, the Encephalomyocarditis virus leader protein:Ran GTPase binding, mediated by the linker-hinge, is a required step in the leader protein-induced nuclear transport inhibition. PMID: 23711384
44. RCC1 facilitates a tight binding between the encephalomyocarditis virus leader and cellular Ran GTPase. PMID: 23536659
45. Reduced RAN expression and disrupted transport between cytoplasm and nucleus are the key events in Alzheimer's disease pathophysiology. PMID: 23308199
46. Reduction in Ran levels causes cytoplasmic decrease and nuclear accumulation of importin alpha leading to cellular senescence in normal cells. PMID: 23160023
47. Results suggest that Ran is required for and is a potential therapeutic target of Myc-driven cancer progression in both breast and lung cancers. PMID: 23468463
48. RanBPM loss of expression results in constitutive activation of the ERK pathway and promotes cellular events leading to cellular transformation and tumorigenesis. PMID: 23118896
49. The increased expression of Ran in lung squamous cell carcinoma might be correlated with carcinogenesis. PMID: 21033436
50. a novel connection between the hyper-activation of the small GTPase Ran and the matricellular protein SMOC-2 that has important consequences for oncogenic transformation. PMID: 22679017
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亞細胞定位：

Nucleus. Nucleus envelope. Cytoplasm, cytosol. Cytoplasm. Melanosome.
蛋白家族：

Small GTPase superfamily, Ran family
組織特異性：

Expressed in a variety of tissues.
數據庫鏈接：

HGNC: 9846

OMIM: 601179

KEGG: hsa:5901

STRING: 9606.ENSP00000376176

UniGene: Hs.10842

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