PRDX2 Recombinant Monoclonal Antibody

說明書

中文名稱：

PRDX2重組抗體
貨號：

CSB-RA339235MA1HU
規格：

￥1320
圖片：
IHC image of CSB-RA339235MA1HU diluted at 1:130 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing Hela cells stained with CSB-RA339235MA1HU (red line) at 1:200. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,020 events was performed.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

產品描述：

CSB-RA339235MA1HU PRDX2重組單克隆抗體是經ELISA、免疫組化（IHC）和流式細胞術（FC）嚴格驗證的高特異性科研工具。該抗體靶向PRDX2蛋白，該蛋白屬于過氧化物氧化還原酶家族，通過清除活性氧參與細胞抗氧化防御，并在細胞凋亡、增殖及信號轉導調控中發揮重要作用，其表達異常與腫瘤進展及神經退行性疾病密切相關。實驗數據顯示，該抗體在IHC應用中能清晰呈現組織樣本中PRDX2的亞細胞定位，在FC分析中可特異性識別細胞表面及胞內靶蛋白，推薦使用稀釋度為1:20-1:200，且不同批次間具有高度穩定性。適用于探究PRDX2在氧化應激調控、腫瘤微環境動態變化及神經退行性病理機制中的分子功能，亦可作為研究相關疾病分子標記物的有效工具，為細胞生物學、病理機制及藥物靶點篩選等基礎研究提供可靠支持。本產品采用重組抗體技術生產，確保高特異性和可重復性，嚴格限于科學研究使用。
Uniprot No.：

P32119
基因名：

PRDX2
別名：

Epididymis secretory sperm binding protein Li 2a antibody; HEL S 2a antibody; MGC4104 antibody; Natural killer cell enhancing factor B antibody; Natural killer cell-enhancing factor B antibody; Natural Killer Enhancing Factor B antibody; NKEF B antibody; NKEF-B antibody; NKEFB antibody; Peroxiredoxin 2 antibody; Peroxiredoxin-2 antibody; PRDX 2 antibody; PRDX2 antibody; PRDX2_HUMAN antibody; PrP antibody; PRX2 antibody; PRXII antibody; PTX1 antibody; TDPX1 antibody; Thiol Specific Antioxidant 1 antibody; Thiol specific antioxidant protein antibody; Thiol-specific antioxidant protein antibody; Thioredoxin Dependent Peroxide Reductase 1 antibody; Thioredoxin peroxidase 1 antibody; Thioredoxin-dependent peroxide reductase 1 antibody; Torin antibody; TPX1 antibody; TSA antibody
反應種屬：

Human
免疫原：

Recombinant Human PRDX2 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Mouse IgG
純化方式：

Affinity-chromatography
克隆號：

4E8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200
FC	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
基因功能參考文獻：
1. Changes in PRDX2 redox/oligomeric states correlated with Obstructive Sleep Apnea severity/metabolic status. Six month of positive airway pressure (PAP) treatment decreased this overoxidation and generated multimeric overoxidized forms associated with chaperone/transduction signaling activity of PRDX2. PMID: 27864139
2. our data suggest that overexpression of peroxiredoxin-2, annexin A2, and heat shock protein beta-1 was correlated with tumor invasion, metastasis, and poor prognosis, and therefore, these proteins may serve as potential diagnostic and therapeutic biomarkers. PMID: 29332450
3. PRX-2 gene can inhibit the phenotypic change of Epidermal Stem Cells differentiating into Sweat Gland Cells and improve the ability to maintain their own specific antigens. PMID: 30070795
4. Prx II exhibited more effective molecular chaperone activity than Prx I when UCH-L1 was the client. Prx II interacted with UCH-L1 through its C-terminal region to protect UCH-L1 from thermal or oxidative inactivation PMID: 29339092
5. The c-Myc/miR-200b/PRDX2 loop regulates colorectal cancer (CRC) progression and its disruption enhances tumor metastasis and chemotherapeutic resistance in CRC. PMID: 29258530
6. Lys191 residue in this exposed C-terminal region of oxidized Prx2 is polyubiquitinated and the ubiquitinated Prx2 is readily degraded in proteasome and autophagy. PMID: 27703196
7. Our findings indicate that Prdx2 might have an important role in the regulation of trophoblast proliferation and apoptosis during early pregnancy, and that its expression is mediated by c-Myc. Thus, these two proteins may be involved in the pathogenesis of RM and may represent potential therapeutic targets PMID: 28661480
8. Data indicate that Prx2 is denitrosylated by Srxn1-mediated, and the two proteins physically bind via disulfide bond formation, providing a structural basis for the enzymatic reaction that requires ATP hydrolysis. PMID: 27821734
9. Overexpression of peroxiredoxin 2 and VEGFR2 in pterygium might be involved in the pathogenesis or recurrence of pterygium. The increase of VEGFR2 might be related to the increase of peroxiredoxin 2 in response to excessive reactive oxygen species from ultraviolet exposure. PMID: 28489720
10. Knockdown of peroxiredoxin 2 (Prdx2) reduced the self-renewal and sphere formation in colon cancer stem cells (CSCs). PMID: 27894099
11. PRDX2 and p-AKT protein expression were analyzed by immunohistochemistry technology. PMID: 28432271
12. Association and oligomerization of Prx II could take part in recovery and protection of the CK BB enzyme activity from inactivation during heat-induced stress. PMID: 29227081
13. Prx II plays a key role in the cancer stem cell self-renewal of hepatocellular carcinoma cells through redox regulation. PMID: 26866938
14. Peroxiredoxin-2 (PRDX2) and hemoglobin-subunits proteins, which are closely involved in the response to oxidative stress. PMID: 27869326
15. High PRDX2 expression is associated with colorectal cancer progression. PMID: 28125800
16. Knockdown of forkhead Box M1 (FoxM1) reduced Prx II levels in H-ras(G12V)-hepatocellular carcinoma (HCC) cells, indicating FoxM1 as a direct transcription factor of Prx II in HCC. PMID: 26500057
17. Prx1 and Prx2 are likely targets of urate hydroperoxide in cells. Oxidation of Prxs by urate hydroperoxide might affect cell function and be partially responsible for the pro-oxidant and pro-inflammatory effects of uric acid. PMID: 28348082
18. Data indicate that peroxiredoxin 2 (PRDX2) was upregulated in white matter multiple sclerosis (MS) lesions mainly in astrocytes, and its expression level was positively correlated with the degree of inflammation and oxidative stress, and suggest that PRDX2 expression contributes to the resistance of astrocytes against oxidative damage. PMID: 28375164
19. The levels of expression of carbonic anhydrase 2, catalase, and PRDX2 in the nipple discharge were significantly increased in breast ductal carcinoma patients as compared to controls. PMID: 26970563
20. Activation of PRX1 and -2 indicate cold atmospheric plasma affects redox homeostasis in osteosarcoma cells PMID: 28314261
21. ig-h3, Peroxiredoxin-2, and NRF2 have roles in cervical carcinogenesis PMID: 28261610
22. Data show that peroxiredoxins PRDX1 and PRDX2 are upregulated in tumor B cells as compared with normal counterparts. PMID: 26636537
23. Data show the protein partners of human Prx1 and Prx2 and identified three sequence motifs, or combination thereof in Prxs partners, namely: CXXC, PXXP, and LXXLL. that can be important for protein localization, function and biological pathways. [review] PMID: 26548861
24. Oxidative stress promotes PRX2 and PRX3 hyperoxidation and attenuates pro-survival signaling in aging chondrocytes. PMID: 26797130
25. Prx2 glutathionylation is a favorable reaction that can occur in cells under oxidative stress and may have a role in redox signaling. GSH/Grx1 provide an alternative mechanism to thioredoxin and thioredoxin reductase for Prx2 recycling. PMID: 26601956
26. the species with one disulfide and one hyperoxidized active site was decameric for Prx2 and dimeric for Prx3. Reduction and re-oxidation of the hyperoxidized dimer of Prx3 produced hyperoxidized monomers PMID: 26614766
27. Functional roles of catalase, PRDX2 and GPX1 during oxidative stress in human erythrocytes. PMID: 25786472
28. Further conclusion showed that Prdx2 regulates VM formation by targeting VEGFR2 activation, which now represents as a therapeutic target for RC. PMID: 25471788
29. Prx II has an important role in cancer cell survival via the modulation of signaling molecules involved in apoptosis and the phosphorylation of JNK by the downregulation of reactive oxygen species levels in A549/GR cells. PMID: 26021759
30. Reported increased expression of ALDH3A1, PDIA3, and PRDX2 in pterygia using a proteomic approach. These proteins are presumed to have a protective role against oxidative stress-induced apoptosis. PMID: 25221425
31. Prx-2 activity is compromised during red blood cell storage. PMID: 25264713
32. two placental proteins, Prx3 and Prx4, may act as new placental immune targets. PMID: 25323516
33. the main role of Prx2 in human erythrocytes is not to eliminate peroxide substrates. PMID: 24952139
34. Our work is the first to show that nuclear levels of PRDX2 display circadian oscillation participating in the regulation of human keratinocytes redox balance. PMID: 24814289
35. Taken together, cloned porcine kidney is more susceptible in JNK-induced apoptosis caused by PrxII phosphorylation, in oxidative stress condition PMID: 24909612
36. the reduced expression of iNOS or peroxiredoxin 2 may play an important role in the carcinogenesis of gastric cancer PMID: 24750185
37. Results suggest that PRDX2 may perform an important function in the pathogeneis of RCMD. PMID: 24862795
38. findings suggest a model in which the release of PRDX2 and TRX from macrophages can modify the redox status of cell surface receptors and enable induction of inflammatory responses PMID: 25097261
39. In this work we characterize Prx2 tyrosine nitration, a post-translational modification on a noncatalytic residue that increases its peroxidase activity and its resistance to overoxidation. PMID: 24719319
40. Prdx2 has an essential role in regulating oxidation-induced apoptosis in colorectal cancer cells. PMID: 24234423
41. Impaired antioxidant activity of Prx2 could contribute to the hemolysis. PMID: 24636884
42. These findings suggest that the transduced PEP-1-PRX2 has neuroprotective functions against oxidative stress-induced cell death in vitro and in vivo PMID: 24631653
43. It documents that the antioxidant protein peroxiredoxin-2 (PRDX2), the third most abundant cytoplasmic protein in RBCs, interacts with the cytoplasmic domain of B3. PMID: 23123411
44. Proteomic data suggest a limited protein set is involved in SET- (template activating factor-I-) mediated cytotoxicity of TCE (trichloroethylene) in hepatocytes; this set includes CFL1 (cofilin 1), PRDX2 (peroxiredoxin 2), and S100-A11 (calgizzarin). PMID: 24631019
45. cellular distribution of Peroxiredoxin I and II in human eyes PMID: 24152995
46. Up-regulation of peroxiredoxin-2 is associated with gemcitabine resistance in pancreatic cancer. PMID: 24222118
47. Data show peroxiredoxin 2 (Prx2) oxidation was detected at erythrocyte:neutrophil ratios found in blood and reversed over time as the oxidative burst subsided. PMID: 23603832
48. Data indicate that engineered Prx2 and Prx3 variants C-terminal residues modulate the extent of hyperoxidation. PMID: 24003226
49. our findings reveal that Prx2 is a key regulator of invasion and metastasis in melanoma PMID: 23749642
50. Hyperoxidized peroxiredoxin 2 interacts with the protein disulfide- isomerase ERp46. PMID: 23713588
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亞細胞定位：

Cytoplasm.
蛋白家族：

Peroxiredoxin family, AhpC/Prx1 subfamily
數據庫鏈接：

HGNC: 9353

OMIM: 600538

KEGG: hsa:7001

STRING: 9606.ENSP00000301522

UniGene: Hs.432121

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