POSTN Recombinant Monoclonal Antibody

說明書

中文名稱：

POSTN重組抗體
貨號：

CSB-RA119753A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA119753A0HU diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.31% DAB.

Overlay Peak curve showing 293T cells stained with CSB-RA119753A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA119753A0HU POSTN重組單克隆抗體是針對細胞外基質蛋白Periostin開發的高特異性科研工具。該抗體采用重組技術制備，具有優異的批間一致性，能夠精準識別人類來源的POSTN蛋白。經嚴格驗證，該產品在酶聯免疫吸附實驗（ELISA）中展現出高親和力，在免疫組織化學（IHC）分析中可清晰顯示蛋白在腫瘤基質或纖維化組織的空間分布，同時在流式細胞術（FC）應用中能有效檢測細胞表面表達。推薦使用濃度范圍為IHC 1:50-1:200、FC 1:50-1:200時均可獲得理想信號強度。Periostin作為細胞遷移與組織重塑的關鍵調控因子，其抗體適用于探索腫瘤微環境、器官纖維化機制及骨發育相關研究，尤其在乳腺癌、非小細胞肺癌等實體瘤的分子機制研究中具有重要價值，也可用于心血管疾病模型中細胞外基質動態變化的檢測。本產品為IgG1同型的小鼠源抗體，適用于體外細胞實驗及石蠟包埋組織樣本的蛋白定位分析。
Uniprot No.：

Q15063
基因名：

POSTN
別名：

Periostin (PN) (Osteoblast-specific factor 2) (OSF-2), POSTN, OSF2
反應種屬：

Human
免疫原：

A synthesized peptide derived from Human POSTN
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

14F2
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Induces cell attachment and spreading and plays a role in cell adhesion. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX.
基因功能參考文獻：
1. Periostin levels were significantly higher in patients with ameloblastoma than in controls. Moreover, Periostin levels in patients with ameloblastoma were significantly associated with the number of disease. Furthermore, the results suggested that Periostin expression significantly promoted the proliferation and migration, in addition to cell cycle progression of AM-1 cells. PMID: 30196986
2. prominent in the superficial dermis in both pemphigus vulgaris and bullous pemphigoid lesions PMID: 27501402
3. Findings indicate that Postn deficiency impairs liver regeneration in mice after partial hepatectomy and Postn might be a novel promoter for liver regeneration. PMID: 28965986
4. Increased serum periostin concentrations are associated with the presence of diabetic retinopathy in patients with type 2 diabetes mellitus. PMID: 29349642
5. Periostin expression is not significantly linked to metastatic disease in pancreatic ductal adenocarcinoma. PMID: 29355490
6. high-level mechanical stretch induces apoptotic cell death, and that periostin plays a protective role against mechanical stretch-induced apoptosis in osteoblastic cells. PMID: 29306496
7. Both periostin and SCCA may play a role in the pathogenesis of acute bronchitis due to respiratory syncytial virus PMID: 29122495
8. serum levels significantly differed between eosinophilic and non-eosinophilic chronic sinusitis with nasal polyps PMID: 29081260
9. Periostin contributes to skin fibrosis by enhancing TGF-beta signaling via Smad 7 inhibition, which may lead to extracellular matrix deposition and periostin generation. PMID: 29433908
10. the occurrence of periostin in the healthy adult human tooth without co-localization with proteins involved in tooth mineralization, the expression of which it regulates PMID: 29289709
11. Periostin is down-regulated in ankylosing spongylitis patients with highly active disease and may contribute to disease pathogenesis through an interaction with Wnt signaling. PMID: 28941122
12. In the Title. PMID: 27423493
13. Our study demonstrates differences in Periostin (PN) expression in histological subtypes of Papillary Thyroid Carcinoma. Our results plead in favor of a dominant protumorigenic role of stromal PN, while the action of epithelial PN is less noticeable PMID: 29435461
14. POSTN may function as a protective factor for osteoblasts during this process by inhibiting the eIF2alphaATF4 pathway. PMID: 29207036
15. Circulating levels of DLL1 and POSN were higher in patients with severe dilated cardiomyopathy and correlated with the degree of diastolic dysfunction. PMID: 28474304
16. High concentrations of serum periostin in acute ICH patients are associated with increasing severity and a poor functional prognosis PMID: 28882488
17. These results demonstrate that POSTN promotes the osteogenic differentiation of mesenchymal stem cells (MSCs) and that CTLA4 enhances the ectopic osteogenesis of MSCs-CTLA4-based tissue-engineered bone. PMID: 28687929
18. measurement of a periostin fragment resulting from in vivo cathepsin K digestion may help to identify subjects at high risk of fracture. PMID: 28766739
19. Study shows that POSTN is present at high levels in ovarian cancer (OC) ascetic fluids, correlates with CD163+ tumor-associated macrophages and, inversely associates with relapse-free survival for OC patients. Overall, data indicate that POSTN is an important factor for macrophage recruitment in the tumor microenvironment and is involved in the interactions between macrophages and ovarian cancer cells. PMID: 29527764
20. confirm associations between periostin and markers of type 2 inflammation, as well as lung function, and identify novel constitutional factors of importance to the use of periostin as a phenotype-specific biomarker in asthma. PMID: 28398635
21. found that POSTN is highly expressed in the bone marrow of human B-ALL patients and mouse models of B-ALL, that deficiency of POSTN significantly decreases leukemia burden in mice and that POSTN directly promotes the proliferation of B-ALL cells PMID: 28529314
22. Results found that elevated circulating periostin level was associated with an increased risk of nonalcoholic fatty liver disease and insulin resistance among overweight and obese individuals. PMID: 27885258
23. Data showed increased POSTN expression in invasive ductal carcinoma (IDC) cells relative to ductal breast carcinoma in situ (DCIS) cells, which may indicate the role of POSTN expression in the IDC cancer cells in the process of tumorigenesis. POSTN high expression is correlated with increasing tumor grade. POSTN expression in the cytoplasm of IDC cancer cells may play an important role in cancer transformation mechanism. PMID: 28902360
24. Data suggest that circulating periostin levels are lower in NAFLD (nonalcoholic fatty liver disease) patients with more severe hepatic fibrosis and, in combination with indices of adiposity (BMI or WC), predict F2-F3 stage. (BMI, body mass index; WC, waist circumference) [LETTER] PMID: 27738886
25. Serum periostin level may play a role in the severity and chronicity of atopic dermatitis in children. PMID: 28631851
26. MicroRNA-599 inhibits proliferation and invasion by down-regulating periostin expression in vitro and in vivo. PMID: 28597372
27. periostin may have a role in obstructive airways disease and its serum distribution may be used as a biomarker PMID: 26917610
28. High expression of periostin and mTOR were independent risk factors of esophageal squamous cell carcinoma patients. PMID: 28665915
29. this study shows that bronchial smooth muscle cells produced periostin after IL-13 stimulation, via the JAK/STAT6, ERK1/2, and PI3K/Akt pathways PMID: 29342461
30. FLG and POSTN expression may be downregulated and upregulated, respectively, in the esophageal mucosa of patients with active eosinophilic esophagitis, and these changes may be restored with treatment in a significant percentage of cases. PMID: 28644349
31. Periostin levels above the median value (25ng/mL) were not related to the time of next exacerbation, time of next COPD hospitalization, (p=0.858) or time to death. The role of serum periostin levels as a predictive biomarker of future risk in hospitalized patients with COPD is of limited value. PMID: 28511944
32. Study shows that overexpressed POSTN was related to epithelial mesenchymal transition (EMT) and tumor progression in head and neck cancer (HNC) clinical samples. These results provide evidence for functional roles of POSTN in the growth, proliferation, metastasis and epithelial mesenchymal transition (EMT) in head and neck cancer. PMID: 29284199
33. We identified periostin as a protein that is enriched in exosomes secreted by metastatic cells and validated its presence in a pilot cohort of breast cancer patient samples with localized disease or lymph node (LN) metastasis. PMID: 27589561
34. Hypoxia promotes glioma-associated macrophage infiltration via periostin and subsequent M2 polarization by upregulating TGF-beta and M-CSFR. PMID: 27602954
35. The relative expression of Periostin is significantly altered in asymptomatic women with subsequent miscarriage compared to women with ongoing pregnancy. Therefore systemic Periostin levels might represent a potential promising biomarker for the assessment of pregnancy outcome. PMID: 29096644
36. Data conclude that periostin may have a role in IGF-1 driven cortical modeling and consolidation in young adults, but it may not be an important mediator in older adults. PMID: 28323143
37. The proteome profiling of uterine fibroids identifies increased extracellular matrix protein expression, in particular periostin, as a hallmark of uterine fibroids regardless of MED12 mutation status. PMID: 29244110
38. High POSTN expression is associated with muscle-invasive bladder cancer. PMID: 26981774
39. POSTN promotes tumor angiogenesis via Erk/VEGF signaling in pancreatic cancer PMID: 27223086
40. urine periostin/creatinine correlated with renal fibrosis and renal outcome in patients with IgA nephropathy PMID: 27802442
41. Periostin increases in response to airway epithelial injury and has been implicated in immunomodulation, mucous production, and extracellular matrix remodeling. PMID: 27905846
42. High POSTN expression is associated with glioblastoma cell invasion. PMID: 26930720
43. By serial deletion mapping of the first FAS I domain, we identified a peptide sequence (amino acids 142-151) of periostin for stimulation of chemotactic migration, adhesion, proliferation and endothelial tube formation of human ECFCs in vitro. Chemotactic migration of ECFCs induced by the periostin peptide was blocked by pre-incubation with an anti-beta5 integrin neutralizing antibody PMID: 29095886
44. high epithelial periostin expression was associated with reduced disease-free survival and overall survival in univariate and multivariate analysis. Periostin may play an important role in the progression of breast tumor, and epithelial periostin expression may serve as a new parameter for prediction of prognosis in patients with invasive breast carcinoma . PMID: 29161296
45. knockdown of POSTN inhibited expression of phosphorylated c-Jun N-terminal kinase PMID: 28833827
46. These results support an important role of periostin in the biology of myeloma and reveal periostin as a possible target for the development of antimyeloma drugs. PMID: 27716740
47. The results indicated that periostin is a pivotal molecule for retinal fibrovascular membrane formation and a promising therapeutic target for the proliferative vitreoretinal diseases. (Review) PMID: 28913545
48. The role of periostin in the pathogenesis of skin diseases has been summarized. (Review) PMID: 28916993
49. Periostin is highly expressed in the lungs of asthmatic patients, contributes to mucus secretion, airway fibrosis and remodeling and is recognized as a biomarker of Th2 high inflammation. (Review) PMID: 28918442
50. Periostin-gene and protein structure has been described. (Review) PMID: 28884327
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亞細胞定位：

Golgi apparatus. Secreted. Secreted, extracellular space, extracellular matrix.
組織特異性：

Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of
數據庫鏈接：

HGNC: 16953

OMIM: 608777

KEGG: hsa:10631

STRING: 9606.ENSP00000369071

UniGene: Hs.136348

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