OCLN Recombinant Monoclonal Antibody

說明書

中文名稱：

OCLN重組抗體
貨號：

CSB-RA293053A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA293053A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing HepG2 cells surface stained with CSB-RA293053A0HU (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

OCLN Recombinant Monoclonal Antibody（CSB-RA293053A0HU）是一款針對緊密連接蛋白Occludin（OCLN）的高特異性抗體，適用于ELISA、免疫組化（IHC）和流式細胞術（FC）檢測。OCLN是維持上皮和內皮細胞緊密連接的關鍵跨膜蛋白，通過調控細胞旁通透性和細胞極性參與屏障功能，其表達異常與感染、炎癥及腫瘤微環境改變密切相關。本抗體經重組表達技術制備，具有高批次一致性，在IHC實驗中可清晰定位OCLN蛋白于細胞膜連接處，推薦使用1:50-1:200稀釋比例；FC檢測中能有效識別細胞表面及胞內OCLN抗原，建議采用相同稀釋范圍。該產品適用于研究上皮/內皮屏障功能調控機制，例如病原體入侵對緊密連接的破壞、炎癥微環境中細胞連接完整性的動態變化，以及腫瘤轉移過程中細胞極性相關信號通路分析。科研人員可將其應用于體外細胞模型、組織切片或原代細胞樣本的OCLN蛋白定量及亞細胞定位研究，為探索細胞間連接相關疾病機制提供可靠工具。
Uniprot No.：

Q16625
基因名：

OCLN
別名：

Occludin, OCLN
反應種屬：

Human
免疫原：

A synthesized peptide derived from human OCLN
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

8H8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.; (Microbial infection) Acts as a co-receptor for hepatitis C virus (HCV) in hepatocytes.
基因功能參考文獻：
1. our findings for the first time identify the role of occludin as a tumor promoter and a prometastatic factor in lung cancer, demonstrating that occludin is a potential prognostic biomarker and therapeutic target in lung cancer PMID: 29750300
2. Occludin protein may contribute to the development of cervical cancer. However, it was not correlated with the clinical features. PMID: 29516973
3. Low occludin expression is associated with Renal cell carcinoma. PMID: 28534944
4. decreased interaction between ZO-1 and occludin might contribute to the epiphora occurred in the transplanted submandibular glands PMID: 28332063
5. integration of claudin-2, occludin and ZO-1 is necessary for maintaining the function of the proximal tubular epithelium. PMID: 29252987
6. Molecular studies of the OCLN gene (NM_001205254) identified six distinct candidate mutations in polymicrogyria. PMID: 28179633
7. Glutamine increased claudin-1 expression in the colonic mucosa of patients with irritable bowel syndrome. In contrast, occludin expression was not significantly modified by glutamine. PMID: 25972430
8. Endothelial cellsTLR4 strongly regulates retinal vessel permeability by reducing expression of occludin and zonula occludens 1. PMID: 29136627
9. Suggest endothelial progenitor cell occluding regulates tube formation, sprouting, and proliferation. PMID: 28681912
10. Viral infectivity was significantly reduced by miR-200c but enhanced by miR-122. PMID: 28642978
11. The mechanism of transition from nondifferentiated to differentiated states in HepaRG cells was studed by proteomics. Two key factors (MMP-14 and OCLN) were validated by qRT-PCR and Western blot. PMID: 27790907
12. Intracellular zinc has an essential role in the maintenance of the intestinal epithelial tight junction barrier through regulation of occludin proteolysis and claudin-3 transcription. PMID: 27151944
13. Data suggest that long noncoding RNA PlncRNA1 and microRNA miR-34c bound together to regulate the expressions of MAZ, ZO-1 and occludin. PMID: 28153728
14. Study provides evidence that occludin contributes to the regulation of size-reductive proliferation and epithelial cell maturation in a phosphorylation-dependent manner. PMID: 27185880
15. Report shows that silencing MARCH3 protects the endothelial barrier and upregulates OCLN in MARCH3-depleted cells. MARCH3 silencing results in the strengthening of cell-cell contacts and inactivates FoxO1. PMID: 27616439
16. STAT3 activation downregulates the ZO-1 and occludin levels and increases the endothelial permeability through the induction of VEGF production in retinal endothelial cells. PMID: 27580405
17. Downregulation of OCLN is associated with clear cell renal cell carcinoma. PMID: 28184927
18. These data suggest that the Rho/ROCK signaling pathway is involved in HIV-1 Tat-mediated changes in occludin, RAGE, and LRP1 in human cerebral microvascular endothelial cells cells. PMID: 27563375
19. OCLN is essential for HCV infection of human hepatic cells. PMID: 26887345
20. OCLN and ZO1 levels appear to be early prognostic markers in patients suffering from sepsis. PMID: 26863122
21. autotypic tight junctions molecular composition, like claudin-1 and occludin expression could influence the demyelinating process by altering the permeability of the blood-nerve barrier. PMID: 26662145
22. Occludin expression has a clear relationship with bone metastasis in human cancer. PMID: 26977027
23. This is report of a family of Indian origin with two affected sibs and segregation of a homozygous novel OCLN mutation in the exon 3(NG_028291.1(OCLN_v001):c.252delC). PMID: 26689621
24. Data show that estrogen mediates control of hepatitis C virus through the G-protein-coupled estrogen receptor 30 (GPR30) pathway leading to cleavage of occludin by Matrix Metalloproteinase-9 (MMP-9). PMID: 26731262
25. In hepatitis C virus -infected human livers, occludin and ADRP mRNA expression levels correlated with each other. Hepatitis C virus increases occludin expression via the upregulation of ADRP. PMID: 26731658
26. Data show that claudin-6 (CLDN6) R209Q and occludin (OCLN) P24A mutations do not affect HCV pseudoparticles (HCVpp) entry. PMID: 26561856
27. resistance to HCV in HIV+ patients may be related to genetic variation in SCARB1 or OCLN PMID: 26571379
28. Angiopoietin-1 Regulates Brain Endothelial Permeability through PTPN-2 Mediated Tyrosine Dephosphorylation of Occludin PMID: 26090670
29. TIMP1 downregulates MMP-2 activity and inhibits the degradation of occluding in Caco-2 intestinal cells PMID: 25665057
30. Study uncovers a novel antiviral effect of miR-122 on human liver cells and shows that over-expression of miR-122 can decrease HCV entry into hepatocytes through down-regulation of OCLN. PMID: 25302477
31. Lipid raft-associated processes, such as PP2A and MMP-2 activation, participate in PCB153-induced disruption of occludin function in brain endothelial barrier. PMID: 26080028
32. miR-18a and RUNX1 could reversely regulate the permeability of blood-tumor barrier as well as the expressions and distributions of ZO-1, occludin and claudin-5. PMID: 25452107
33. Zonula occludens-1, occludin and E-cadherin expression and organization in salivary glands PMID: 25248927
34. LL-37 selectively increased the expression of several claudins and occludin, and enhanced their membrane distribution. PMID: 24862212
35. In conclusion, our present study indicated that miR-34c regulated the permeability of BTB via MAZ-mediated expression changes of ZO-1, occludin, and claudin-5. PMID: 25201524
36. findings suggest that cerebral vascular cells express functional BACE1. Moreover, elevated vascular BACE1 may contribute to deficiency of occludin in caa. PMID: 24739782
37. luciferase assays and chromatin immunoprecipitation assays showed that KLF4 up-regulated the promoter activities and interacted with "CACCC" DNA sequence presented in the promoters of ZO-1, occludin, and claudin-5. PMID: 24318462
38. We conclude that OCEL-mediated occludin interactions are essential for limiting paracellular macromolecular flux. PMID: 23924897
39. Data suggest components of diet supplements (here, glutamine/arginine) can improve permeability and tight junction protein expression (OCLN/zona occludens 1) in enterocytes exposed to deleterious effects of antineoplastic agents (here, methotrexate). PMID: 23428392
40. Eosinophilic esophagitis (EoE), an inflammatory atopic disease of the esophagus, causes massive eosinophil infiltration, basal cell hyperplasia, and sub-epithelial fibrosis. Western analysis of pinch biopsies from EoE and normal pediatric patients indicated reduced expression of intercellular junction proteins, E-cadherin and claudin-1 and increased expression of occludin and vimentin. PMID: 23792687
41. actin cytoskeletal dynamics is detrimental to METH-induced BBB dysfunction by increasing internalization of occludin. PMID: 24081143
42. identification of a novel deletion/rearrangement of OCLN that results in band-like brain calcification, limited cognitive abilities, failure to thrive and renal dysfunction in early childhood PMID: 23793442
43. In HepG2 cells, both hepatitis C virus cell entry and tight junction formation were impaired by OCLN silencing and restored by expression of antibody regulatable OCLN mutant. PMID: 23555257
44. Loss of occludin is involved in adhesion, apoptosis, differentiation, Ca2+-homeostasis and neoplastic transformation in UV-exposed human keratinocytes and in skin neoplasms. PMID: 23390516
45. Sinonasal epithelium in allergic fungal rhinosinusitis displays increased epithelial permeability and an altered expression of occludin. PMID: 22927233
46. HuR promotes occludin translation by blocking occludin mRNA translocation to P-bodies via the displacement of CUGBP1. PMID: 23155001
47. It is concluded that attenuated expression of the TJ proteins occludin and ZO-1 in human gastric epithelial cells could be involved in clopidogrel-induced gastric mucosal injury through activation of the p38 MAPK pathway PMID: 23220562
48. Elevated occludin expression is associated with active ulcerative colitis. PMID: 22134947
49. TTF-1 transcriptionally regulates occludin PMID: 22761434
50. We define the interface between the ZO-1 PDZ3-SH3-U5-GuK (PSG) and occludin coiled-coil (CC) domains. PMID: 22711802
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相關疾病：

Pseudo-TORCH syndrome 1 (PTORCH1)
亞細胞定位：

Cell membrane; Multi-pass membrane protein. Cell junction, tight junction.
蛋白家族：

ELL/occludin family
組織特異性：

Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
數據庫鏈接：

HGNC: 8104

OMIM: 251290

KEGG: hsa:100506658

STRING: 9606.ENSP00000347379

UniGene: Hs.592605

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