MCM3 Recombinant Monoclonal Antibody
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中文名稱(chēng)：MCM3 Recombinant Monoclonal Antibody
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貨號(hào)：CSB-RA261449A0HU
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規(guī)格：￥1320
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圖片：
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IHC image of CSB-RA261449A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
IHC image of CSB-RA261449A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Overlay Peak curve showing jurkat cells stained with CSB-RA261449A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產(chǎn)品詳情
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Uniprot No.：
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基因名：
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別名：DNA replication licensing factor MCM3 (EC 3.6.4.12) (DNA polymerase alpha holoenzyme-associated protein P1) (P1-MCM3) (RLF subunit beta) (p102), MCM3
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反應(yīng)種屬：Human
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免疫原：A synthesized peptide from human MCM3 protein
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類(lèi)型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號(hào)：10A2
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, IHC, FC
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推薦稀釋比：
Application Recommended Dilution IHC 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
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基因功能參考文獻(xiàn)：
- this study shows that MCM3 is a novel proliferation marker in oral squamous cell carcinoma PMID: 27258565
- High MCM3 expression is associated with bone metastasis and advanced human prostate cancer. PMID: 28424404
- reveal the Minichromosome Maintenance Complex to be a critical and directly regulated node within the miR-183 signaling network in MYCN-amplified neuroblastoma cells. Randomly selected MCMs 3 and 5 were experimentally confirmed as direct targets of miR-183. PMID: 27239679
- The results of our preliminary study emphasize the need for future research on MCM-3 as a sensitive proliferation marker, providing an alternative to Ki-67, in cases of various major salivary gland epithelial tumors in children and adolescents. PMID: 28547962
- These data establish new functions for KEAP1 within the nucleus and identify MCM3 as a novel substrate of the KEAP1-CUL3-RBX1 E3 ligase. PMID: 27621311
- The aim of this study is to analyze the immunoexpression of Ki67, p53, MCM3 and PCNA markers in epithelial remnants of dental follicles of impacted teeth and to identify a possible correlation between the immunoexpression of these markers in dentigerous cysts and keratocystic odontogenic tumors. PMID: 27516012
- High MCM-3 expression correlated with Cutaneous T-cell Lymphomas. PMID: 26504025
- normal DNA replication and replication checkpoint activation is regulated through the novel phosphorylation of MCM3 by Chk1 PMID: 25809478
- The relatively lower MCM3 protein expression in FVPTC. PMID: 23821456
- Expression of KB cell MCM3 was not affected by doxorubicin. PMID: 24344040
- present study aimed to investigate the relationship between expression of minichromosome maintenance proteins (MCM-3, MCM-7), metallothioneins (MT-I/II, MT-III), and Ki-67 in 103 ovarian cancer cases, mostly of the serous histological type PMID: 24324072
- High MCM3 expression is associated with mucoepidermoid carcinomas and adenoid cystic carcinomas in salivary gland tumors. PMID: 23886132
- Relatively lower MCM3 protein expression in follicular variant of papillary thyroid carcinoma comparing to classical type could be due to a different tumorigenic pathway favored in this type of tissue. PMID: 23821456
- These findings provide validation of the utility of MCM3 expression as an independent biomarker for prognostication of patients with primary melanoma PMID: 22805320
- excess of MCM3 up-regulates the phosphorylation of CHK1 Ser-345 and CDK2 Thr-14. PMID: 21965652
- MCM3 gene expression is decreased in follicular variant of papillary thyroid carcinoma, contrary to expectation. PMID: 21509594
- Using U373MG cells we found that HCMV IE86 protein was bound to Mcm3, but did not inhibit the cellular DNA synthesis. PMID: 20545442
- MCM3 may be a more reliable proliferation marker than Ki-67 in accessing the growth of tumor and evaluating tumor aggressiveness of papillary thyroid carcinoma. PMID: 19818763
- The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with this protein PMID: 12226073
- Elevated MCM3 protein expression is associated with neoplasms PMID: 15623617
- MCM3 is overexpressed in human astrocytic tumors and elicits a cancer-restricted humoral immune response in some patients with brain neoplasms PMID: 15671553
- ORC1 and Mcm3 proteins are required for DNA replication in protein extracts from human cells PMID: 16537544
- analysis of carboxyl-terminal MCM3 phosphorylation sites and their motifs PMID: 17244605
- These data reveal that CDK-dependent MCM3 phosphorylation contributes to the regulated formation of the MCM2-7 complex. PMID: 18524952
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亞細(xì)胞定位：Nucleus. Chromosome.
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蛋白家族：MCM family
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